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Design hardware calculator in PWM peripherals of nios Ⅱ with verilog HDL

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重庆邮电大学学报（自然科学版） 2009年第2期21卷 296-299页

作者： xu yang xiang Min College of Automation Chongqing University of Posts and Telecommunication Chongqing 400065 P. R. China

The Plus Width Module (PWM) in microprocessors is commonly used in motor control field. If the ordinary PWM peripherals output PWM waveform, the software must calculate the period and duty cycle number based on the microprocessor’s system clock cycle first, and then write them into the registers. As all known, the microprocessor without hardware calculator consume very long time in multiplication and division. In order to reduce the CPU’s running time, a calculation hardware unit was designed to calculate the waveform parameters. The software only need to write the period (Hz units) to the period register and the duty cycle (% units)to its register. This hardware unit was designed with Verilog HDL and running in the Nios Ⅱ system successfully.

关键词： Verilog PWM波形 HDL语言硬件设计计算机外设微处理器计算单位

Analysis of Genetic Diversity in Cultivated and Wild Tomato Varieties in Chinese Market by RAPD and SSR

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Agricultural Sciences in China 2010年第10期9卷 1430-1437页

作者： MENG Fan-juan xu xiang-yang HUANG Feng-lan LI Jing-fu College of Life Science University of Northeast Forestry Harbin 150040 P.R.China College of Horticulture Northeast Agricultural University Harbin 150030 P.R.China College of Life Science Inner Mongolia University for Nationalities Tongliao 028000 P.R. China

RAPD and SSR were applied to assess genetic diversity in 61 tomato varieties from different species （Solanum lycopersicum L., hirsutum. Humb L., pimpinellifolium Miller L., chilense Dun. L., chmielenskii L., peruvianum Miller L., parvuflorum Miller L.）. 2 062 and 869 clear fragments were amplified by RAPD and SSR, respectively. On the other hand, more polymorphic products were found by SSR as compared to RAPD, i.e., 100 and 43.84%, respectively. In addition, a higher value of the average similarity coefficient and lower PIC value were reflected in RAPD （0.79, 0.407） compared to SSR （0.56, 0.687）. It can be inferred that SSR was a higher effective marker than RAPD to assess genetic diversity in tomato accessions. Similarly, the genetic base of tomato varieties in Chinese market was narrow. It is suggested that wild tomato varieties should be used to enrich the genetic base of the cultivated tomato varieties.

关键词： tomato genetic diversity RAPD SSR

Genome-wide identification and expression analysis of GDSL esterase/lipase genes in tomato

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Journal of Integrative Agriculture 2022年第2期21卷 389-406页

作者： SUN Yao-guang HE Yu-qing WANG He-xuan JIANG Jing-bin yang Huan-huan xu xiang-yang School of Horticulture and Landscape Architecture Northeast Agricultural UniversityHarbin 150030P.R.China

The GDSL esterase/lipase family contains many functional genes that perform important biological functions in growth and development, morphogenesis, seed oil synthesis, and defense responses in plants. The expression of GDSL esterase/lipase genes can respond to biotic and abiotic stresses. Although GDSL esterase/lipase family genes have been identified and studied in other plants, they have not been identified and their functions remain unclear in tomato. This study is the first to identify 80 GDSL esterase/lipase family genes in tomato, which were named SlGELP1–80. These genes were mapped to their positions on the chromosomes and their physical and chemical properties, gene structure, phylogenetic relationships, collinear relationships, and cis-acting elements were analyzed. The spatiotemporal expression characteristics of the Sl GELP genes in tomato were diverse. In addition, RNA-seq analysis indicated that the expression patterns of the SlGELP genes in tomato differed before and after inoculation with Stemphylium lycopersici. qRT-PCR was used to analyze the expression of five Sl GELP genes after treatments with S. lycopersici, salicylic acid and jasmonic acid. Finally, this study was the first to identify and analyze GDSL esterase/lipase family genes in tomato via bioinformatics approaches, and these findings provide new insights for improving the study of plant disease resistance.

关键词： GDSL esterase/lipase gene family identification plant disease resistance gray leaf spot Solanum lycopersicum

Technical Study on Ultra-Low Sulfur Steel Production in Ansteel

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Journal of Iron and Steel Research International 2011年第S1期18卷 567-571页

作者： xu xiang-yang (No.1 Steelmaking Plant of Angang Steel Co Ltd.,Anshan 114031,China) 1 Steelmaking Plant Angang Steel Co Ltd Anshan 114031 Peoples R China

Based on the fact of long period deep desulfurization treatment in LF,the relationships among top slag constituent in LF,molten steel constituent,stirring ability of blowing argon,molten steel temperature and desulphurization rate were *** the experiments,the parameters about treatment technology of top slag in LF,the [Als] content in molten steel,slag charge match,molten steel temperature and the argon flow for stirring have been *** desulphurization treatment period in LF can be shortened by 5～8 *** target sulfur content in molten steel can be controlled below 30 ppm within one LF treatment period which is only 36 *** LF treatment period of ultra-low sulfur steel can primarily match with the continuous casting period,multi-heat continuous casting can be ensured.

关键词： ultra-low sulfur steel LF deep desulphurization treatment

Subacute toxicity of exogenous manganese on rat hippocampal neurons Examination by MRI and optical microscopy

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Neural Regeneration Research 2008年第5期3卷 550-553页

作者： Xiaoli xu Yun xiang Min yang School of Medicine ＆ Life Science Jianghan University Wuhan 430056 Hubei Province China

BACKGROUND： Manganism may cause learning and memory impairment by influencing the normal function of the hippocampus, however, this effect requires further examination. OBJECTIVE： To investigate the effects of manganism on the rat hippocampus using immunohistochemistry and MRI examination. DESIGN, TIME AND SETTING： A randomized controlled study, performed in the School of Medicine and Life Science, Jianghan University and the State Key Laboratory of Atomic ＆ Molecular Physics and Spectroscopy, Chinese Academy of Science, from July to September 2005. MATERIALS： Fourteen healthy SD rats aged two months were selected for this study. MnCl2 4H2O （BHD, UK）（batch number： 9791325）; glial fibrillary acidic protein （GFAP） staining kit （Beijing Zhongshan Biotechnology）; Biospec 4.7T/30 animal MRI formatter （Bruker, Germany）. METHODS： Fourteen rats were randomly divided into a control group （n =7） and a manganism group （n = 7）. Rats in the manganism group received intraperitoneal injection of MnCl2 · 4H2O （50 mg/kg）, once a day, for four successive days. Rats in the control group were injected according to the manganism regimen, but using saline instead of manganese solution. MAIN OUTCOME MEASURES： Twenty-four hours after the last injection, rats were examined using MRI. Immunohistochemically stained GFAP and hematoxylin-eosin stained hippocampal sections were observed under optical microscopy. RESULTS： Fourteen rats were included in the final analysis. After manganese treatment, T1 weighted image and inversion recovery MR1 demonstrated that the signal intensity was significantly enhanced in hippocampus, compared to controls. Neuronal necrosis was not observed in the hippocampus after HE staining. As compared to the control group, GFAP expression was markedly enhanced in the hippocampus of the manganism group. CONCLUSION： Within the rat brain, manganese preferentially localizes to the hippocampus and can induce astroctye activation.

关键词： manganism hippocampus rats MRI glial fibrillary acidic protein

Silencing the SLB3 transcription factor gene decreases drought stress tolerance in tomato

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Journal of Integrative Agriculture 2020年第11期19卷 2699-2708页

作者： WANG Zi-yu BAO Yu-fang PEI Tong WU Tai-ru DU xu HE Meng-xi WANG Yue LIU Qi-feng yang Huan-huan JIANG Jing-bin ZHANG He LI Jing-fu ZHAO Ting-ting xu xiang-yang College of Horticulture and Landscape Architecture Northeast Agricultural UniversityHarbin 150030P.R.China Key Laboratory of Biology and Genetic Improvement of Horticultural Crops(Northeast Region) Ministry of Agriculture and Rural AffairsHarbin 150030P.R.China

BRI1-EMS-SUPPRESSOR 1(BES1)transcription factor is closely associated with the brassinosteroid(BR)signaling pathway and plays an important role in plant growth and ***3 is a member of BES1 transcription factor family and its expression was previously shown to increase significantly in tomato seedlings under drought *** the present study,we used virus-induced gene silencing(VIGS)technology to downregulate SLB3 expression to reveal the function of the SLB3 gene under drought stress *** downregulated expression of SLB3 weakened the drought tolerance of the plants appeared earlier wilting and higher accumulation of H2 O2 and O2^–·,decreased superoxide dismutase(SOD)activity,and increased proline(PRO)and malondialdehyde(MDA)contents and peroxidase(POD)*** real-time PCR(qRT-PCR)analysis of BR-related genes revealed that the expression of SlCPD,SlDWARF and BIN2-related genes was significantly upregulated in SLB3-silenced seedlings under drought stress,but that the expression of TCH4-related genes was *** results showed that silencing the SLB3 gene reduced the drought resistance of tomato plants and had an impact on the BR signaling transduction which may be probably responsible for the variation in drought resistance of the tomato plants.

关键词： SLB3 BES1 transcription factor VIGS drought gene expression BR signaling pathway

Cross-Cultural Pragmatic Failure in Business English Interpretation

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大众商务（下半月） 2009年第12期 198-199页

作者： YU xu - xiang yang Ke （School of Foreign Languages for Finance and Economics Southwestern University of Finance and Economics Chengdu611130 China）

Low-Cost and Large-Scale Clean Steel Production Technology

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Journal of Iron and Steel Research International 2011年第S2期18卷 52-58页

作者： MA Cheng,ZANG Shao-shuang,MA Yong,ZHANG Xiao-jun,xu Yan-hao,xu xiang-yang,NING Dong(Steelmking Plant of Angang Steel Co Ltd,Anshan 114021,Liaoning,China) Steelmking Plant Angang Steel Co Ltd Anshan 114021 Liaoning Peoples R China

As the market demand for clean steel increased,a number of new technologies for clean steel production have been developed in some domestic and foreign steelmaking *** on the existing equipments and technical conditions in the No.1 steelmaking plant of Ansteel,a series of clean steel technologies have been integrated including single bay duplex-dephosphorization in steelmaking control technology,high efficiency desulfurization technology,the control technology for nitrogen content during the whole process,the control technology for hydrogen content in VD,and the systematic control technology for oxygen content.A series of process for all kinds of ultra-low phosphorus,ultra-low sulfur and relative low level of inclusions have been designed in clean steel *** process include hot metal desulfurization and slag-off,single bay duplex-dephosphorization in BOF,molten steel slag-off,LF processing,VD processing,and continuous casting with protection,then a stable,low-cost and large-scale production process has been *** this integrative technology,the sum of 5 major elements including phosphorus,sulfur,oxygen,nitrogen,hydrogen is less than 80×10-6 in high alloy steel scale production,and the industrial applications for clean steel technology have been achieved.

关键词： clean steel single bay duplex dephosphorization scalization

Downregulation of SL-ZH13 transcription factor gene expression decreases drought tolerance of tomato

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Journal of Integrative Agriculture 2019年第7期18卷 1579-1586页

作者： ZHAO Ting-ting WANG Zi-yu BAO Yu-fang ZHANG Xiao-chun yang Huan-huan ZHANG Dong-ye JIANG Jing-bin ZHANG He LI Jing-fu CHEN Qing-shan xu xiang-yang Laboratory of Genetic Breeding in Tomato College of Horticulture and Landscape ArchitectureNortheast Agricultural UniversityHarbin 150030P.R. China College of Agronomy Northeast Agricultural UniversityHarbin 150030P.R. China

Zinc finger-homeodomain proteins(ZF-HDs) are transcription factors that regulate plant growth,development,and abiotic stress *** SL-ZH13 gene was found to be significantly upregulated under drought stress treatment in tomato(Solanum lycopersicum) leaves in our previous *** this study,to further understand the role that the SL-ZH13 gene plays in the response of tomato plants to drought stress,the virus-induced gene silencing(VIGS) method was applied to downregulate SL-ZH13 expression in tomato plants,and these plants were treated with drought stress to analyze the changes in drought *** SL-ZH13 silencing efficiency was confirmed by quantitative real-time PCR(qRT-PCR) *** SL-ZH13-silenced plants,the stems wilted faster,leaf shrinkage was more severe than in control plants under the same drought stress treatment conditions,and the mean stem bending angle of SL-ZH13-silenced plants was smaller than that of control *** analyses showed that the activity of superoxide dismutase(SOD) and peroxidase(POD) and the content of proline(Pro) in SL-ZH13-silenced plants were lower than those in control plants after 1.5 and 3 h of drought stress *** malondialdehyde(MDA) content in SL-ZH13-silenced plants was higher than that in control plants after 1.5 and 3 h of drought stress treatment,and H2O2 and O2^-· accumulated much more in the leaves of SL-ZH13-silenced plants than in the leaves of control *** results suggested that silencing the SL-ZH13 gene affected the response of tomato plants to drought stress and decreased the drought tolerance of tomato plants.

关键词： SL-ZH13 gene VIGS drought stress tomato transcription factor