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Gold-nanoparticle-based multiplex immuno-strip biosensor for simultaneous determination of 83 antibiotics

Nano Research 2023年第1期16卷 1259-1268页

作者： Xianlu Lei Xinxin xu Liqiang Liu liguang xu Li Wang Hua Kuang Chuanlai xu State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China

Antibiotic residues,generated by the irrational use of drugs and environmental pollution,have always been a great challenge to aquaculture ***,a quick,convenient,and performance-excellent way to detect antibiotic residues in aquaculture fish is urgently *** this study,a multiplex immunochromatographic strip biosensor based on gold nanoparticles was developed for the simultaneous detection of five classes of antibiotic residues(24β-lactam antibiotics,26 sulfonamides,five tetracyclines,24 quinolones,and four amphenicols)in aquaculture fish within 10 *** detection ranges of five representative antibiotics,penicillin G,sulfamethazine,tetracycline,enrofloxacin,and chloramphenicol,were 2.33–38.4,0.688–17.1,1.4–48.1,1.45–32.9,and 0.537–9.06μg/kg,*** accuracy and stability of these measurements were demonstrated by analyzing spiked fish samples,with recovery rates of 87.5%–115.2%and a coefficient of variation<9.5%.The cross-reaction rates,based on the five representative antibiotics,were 3.77%–202%forβ-lactams,3.95%–137%for sulfonamides,9.19%–100%for tetracyclines,4.9%–145%for quinolones,and 3.2%–100%for *** excellent testing performance of the biosensor strip to most of antibiotic residues in aquaculture fish ensures they meet the maximum residue limits required by countries or ***,this multiplex immunochromatographic strip biosensor is potentially applicable to the rapidly on-site determination of antibiotic residues in aquaculture fish.

关键词： antibiotic aquaculture biosensor immunochromatographic strip

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Gold-based lateral-flow strip for the detection of penconazole in watermelon and cucumber samples

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Food Quality and Safety 2022年第2期6卷 257-264页

作者： Yang Liu Xinxin xu Liqiang Liu liguang xu Hua Kuang Chuanlai xu State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxiChina International Joint Research Laboratory for Biointerface and Biodetection School of Food Science and TechnologyJiangnan UniversityWuxiChina

As a typical triazole fungicide,penconazole(PEN)is widely used in agriculture but has been proven to be *** this study,we designed a new hapten to prepare a highly sensitive and specific anti-PEN monoclonal antibody(mAb)and established a gold nanoparticle-based lateral-flow immunoassay(LFIA)for the detection of PEN residues in watermelon and *** 50%inhibitory concentration(IC_(50))of the mAb was 0.42 ng/mL and the LFIA strip had a visual limit of detection(vLOD)of 2.5 ng/g and a cut-off value of 10 ng/g in watermelon and *** calculated limit of detection(LOD)of the LFIA strip was 0.36 ng/g for watermelon and 0.29 ng/g for *** LFIA strip also gave a re-covery rate of 92.5%-109.0%for watermelon samples and 92.5%-106.7%for cucumber *** results using the LFIA strip are highly consistent with those seen using liquid chromatography-tandem mass *** our developed LFIA strip represents a potentially reliable tool for the rapid on-site screening for PEN in watermelons and cucumbers.

关键词： Penconazole monoclonal antibody lateral flow immunoassay watermelon cucumber

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Multiple detection of 15 triazine herbicides by gold nanoparticle based-paper sensor

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Nano Research 2022年第6期15卷 5483-5491页

作者： Lingling Guo Xinxin xu Jing Zhao Shudong Hu liguang xu Hua Kuang Chuanlai xu State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China Department of Radiology Affiliated HospitalJiangnan UniversityWuxi 214122China

Triazine herbicides have been widely used in agriculture,but their residues can harm the environment and human *** help monitor these,we have developed an effective immunochromatographic strip test that can simultaneously detect 15 different triazines in grain samples(including ametryn,cyprazine,atraton,prometon,prometryn,atrazine,propazine,terbuthylazine,simetryn,trietazine,secbumeton,simazine,desmetryn,terbumeton and simetone).Based on our optimization procedure,the visual limit of detection(vLOD)for these triazines was found to be 2–10 ng/mL in assay buffer,and 0.02–0.1 mg/kg in grain *** different grain matrices including corn,brown rice,wheat,and sorghum were studied and the test results showed no significant differences between the 15 triazines analyzed using this *** test is simple,convenient,rapid,and low-cost,and could be an effective tool for primary screening of triazine residues in grain samples.

关键词： triazine herbicides immunochromatographic strip gold nanoparticles grain

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Circularly Polarized Light-Enabled Chiral Nanomaterials:From Fabrication to Application

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Nano-Micro Letters 2023年第3期15卷 171-189页

作者： Changlong Hao Gaoyang Wang Chen Chen Jun xu Chuanlai xu Hua Kuang liguang xu International Joint Research Laboratory for Biointerface and Biodetection State Key Lab of Food Science and TechnologySchool of Food Science and TechnologyJiangnan UniversityWuxiJiangsu 214122People’s Republic of China Department of Neurology China National Clinical Research Center for Neurological DiseasesBeijing Tiantan HospitalCapital Medical UniversityNo.119 South 4Th Ring West RoadBeijing 100070People’s Republic of China

For decades,chiral nanomaterials have been extensively studied because of their extraordinary *** nanostructures have attracted a lot of interest because of their potential applications including biosensing,asymmetric catalysis,optical devices,and negative index *** polarized light(CPL)is the most attractive source for chirality owing to its high availability,and now it has been used as a chiral source for the preparation of chiral *** this review,the recent progress in the field of CPL-enabled chiral nanomaterials is ***,the recent advancements in the fabrication of chiral materials using circularly polarized light are described,focusing on the unique ***,an overview of the potential applications of chiral nanomaterials driven by CPL is provided,with a particular emphasis on biosensing,catalysis,and ***,a perspective on the challenges in the field of CPL-enabled chiral nanomaterials is given.

关键词： Circularly polarized light Chiral Nanomaterials Fabrication Application

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A quadruplex immunochromatographic assay for the ultrasensitive detection of 11 anesthetics

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Nano Research 2023年第8期16卷 11269-11277页

作者： Xianlu Lei Xinxin xu Li Wang Wei Zhou Liqiang Liu liguang xu Hua Kuang Chuanlai xu State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China Jiangsu Product Quality Testing and Inspection Institute Nanjing 210000China

Anesthetic residues in fish represent a potential risk to human ***,it is important to develop a sensitive and broad-specific method for the detection of *** this study,we developed a colloidal gold-based quadruplex immunochromatographic(Qua-ICS)assay using four highly sensitive monoclonal antibody immunotherapy(mAbs)that simultaneously detected 11 anesthetic residues in fish within 10 *** colorimetric and cut-off values(COVs)for procaines,eugenols,bupivacaines,and tricaine(TMS)were 0.37–1.1 and 3.3–10,11–222 and 100–2000,0.37 and 3.3,and 111 and 10,000µg/kg,*** analysis was achieved with a portable strip-reader,and the detection ranges were 0.15–2.6,6.3–677,0.13–2.8,and 83–1245µg/kg for procaines,eugenols,bupivacaines,and tricaine,*** developed method was reliable and accurate according to the recovery test results and analyses of real ***,the strip can be used as an alternative method for the rapid detection of anesthetic residues in fish.

关键词： bupivacaine colloidal gold eugenol procaine tricaine(TMS)

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Synthesis of haptens and gold-based immunochromatographic paper sensor for vitamin B_(6) in energy drinks and dietary supplements

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Nano Research 2022年第3期15卷 2479-2488页

作者： Lu Zeng Xinxin xu Shanshan Song liguang xu Liqiang Liu Jing Xiao Chuanlai xu Hua Kuang State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China International Joint Research Laboratory for Biointerface and Biodetection and School of Food Science and TechnologyJiangnan UniversityWuxi 214122China NHC Key Laboratory of Food Safety Risk Assessment China National Center for Food Safety Risk AssessmentBeijing 100022China

We designed and synthesized novel haptens to produce monoclonal antibodies(mAb)against vitamin B_(6)(VB_(6)).A group-specific mAb(2F9)that recognized pyridoxine(PN),pyridoxamine(PM),and pyridoxal(PL)was prepared using a homologous strategy with 50%maximal inhibitory concentration(IC_(50))values of 106.60,250.57,and 400.11 ng/mL,*** on this,a gold nanoparticles(AuNPs)-based immunochromatographic strip(ICS)test was established for the detection of VB_(6) in energy drinks and B-vitamin complex *** developed ICS test results could be semi-quantitatively evaluated by the naked eye within 10 min,and displayed the visual limit of detection(vLOD)values of 250,500,and 1,000 ng/mL for PN,PM,and PL,*** quantitative analysis,the results obtained by strip reader,with calculated LOD values for PN,PM,and PL were 14.10,55.58,and 56.25 ng/mL,*** energy drinks and B-vitamin complex tablet samples were detected by the strips and the results were confirmed with high-performance liquid ***,the developed AuNPs-based immunochromatographic sensor was suitable and promising for the group-specific recognition and rapid detection of VB6 in fortified foods and dietary supplements.

关键词： vitamin B_(6) monoclonal antibody immunochromatographic strip test

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Gold nanoparticle-based paper sensor for ultrasensitive and multiple detection of 32 （fluoro）quinolones by one monoclonal antibody

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Nano Research 2017年第1期10卷 108-120页

作者： Juan Peng Liqiang Liu liguang xu Shanshan Song Hua Kuang Gang Cui Chuanlai xu State Key Laboratory of Food Science and Technology Jiangnan University Wuxi 214122 China School of Food Science and Technology Jiangnan University Wuxi 214122 China

For rapid and simultaneous detection of （fluoro）quinolones, a broadly specific monoclonal antibody （mAb） that recognizes 32 （fluoro）quinolone antibiotics was prepared using a mixture of a norfloxacin derivative and a sarfloxacin derivative as the hapten. An immunochromatographic strip based on gold nanoparticles （AuNPs） was then assembled with goat anti-mouse antibody and antigen （sarfloxacin coupled to ovalbumin）, used to form the C line and T line, respectively. This antigen competes with the （fluoro）quinolones in a sample incubated with mAbs labeled with AuNPs. The strip can detect 32 （fluoro）quinolones including oxolinic acid, nalidixic acid, miloxacin, pipemidic acid, piromidic acid, rosoxacin, cinoxacin, norfloxacin, pefloxacin, lomfloxacin, enofloxacin, fleroxacin, ciprofloxacin, enrofloxacin, dafloxacin, orbifloxacin, sparfloxacin, gemifloxacin, besifloxacin, balofloxacin, gatifloxacin, moxifloxacin, nadifloxacin, ofloxacin, marbofloxacin, flumequine, pazufloxacin, prulifloxacin, sarafloxacin, difloxacin, trovafloxacin, and tosufloxacin in milk within 10 min with the naked eye. The cut-off values of the strip range from 1 to 100 ng/mL and the limits of detection are 0.1- 10 ng/mL. The strip does not cross-react with antibiotics including tetracycline, sulfamethazine, ampicillin, erythromycin, aflatoxin B1, or gentamicin. In short, this immunochromatographic strip is a very useful tool for the primary screening of （fluoro）quinolones in milk.

关键词： (fluoro)quinolones gold nanoparticle monoclonal antibody paper sensor

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Gold immunochromatographic sensor for the rapid detection of twenty-six sulfonamides in foods

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Nano Research 2017年第8期10卷 2833-2844页

作者： Yanni Chen Liqiang Liu liguang xu Shanshan Song Hua Kuang Gang Cui Chuanlai xu State Key Laboratory of Food Science and Technology Jiangnan University Wuxi 214122 China School of Food Science and Technology Jiangnan University Wuxi 214122 China

A gold immunochromatographic sensor （GICS） was developed for the rapid detection of 26 sulfonamides in honey samples. The sensor was based on a group-specific monoclonal antibody （mAb） that can recognize all 26 sulfonamides. Three haptens （hapten I with a thiazole ring, hapten 2 with a benzene ring, and hapten 3 with a straight carbon chain） were used for antigen preparation. With hybridoma technology, a group-specific mAb was screened with a 50% maximal inhibitory concentration （IC50） against sulfathizole （STZ） and the other 25 analogues ranging from 0.08 to 90.18 ng/mL. Mono-dispersed gold nanoparticles were conjugated with the mAb to develop the lateral immunochromatographic strip. A labeled antibody concentration of 0.1 pg/mL and a coating antigen concentration of 0.2 μg/mL in the test line were chosen for strip preparation. Under optimized conditions, the visual limits of detection （vLOD） for the concentrations of STZ, sulfamethoxazole, sulfamethizole, sulfadiazine, sulfamerazine, sulfadimethoxine, sulfamonomethoxine, sulfameter, sulfamethoxypyridazine, and sulfachloropyridazine were 5, 0.25, 0.25, 10, 5, 10, 25, 2.5, 5, 0.25, and 10 μg/kg, respectively. Scanner analysis in honey samples revealed good performance for detection of the 26 sulfonamides. Commercial honey samples were tested with the sensor and positive results were confirmed with high-performance liquid chromatography. The proposed strip sensor provides a convenient method for the rapid and reliable determination of sulfonamides pollutants in honey samples.

关键词： sulfonamides gold immunochromatographic sensor monoclonal antibody honey samples

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Gold nanoparticle-based lateral flow immunoassay for the rapid and on-site detection of wheat allergen in milk

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Food Bioscience 2023年第1期51卷 1212-1219页

作者： Jialin Hu Xinxin xu liguang xu Hua Kuang Chuanlai xu Lingling Guo State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxiJiangsu214122China International Joint Research Laboratory for Biointerface and Biodetection School of Food Science and TechnologyJiangnan UniversityWuxiJiangsu214122China

Wheat gliadin is one of the most important causes of gluten *** study aimed to develop a sandwich enzyme-linked immunosorbent assay(ELISA)and gold nanoparticle-based lateral flow(LFIA)strips to detect wheat allergen in *** cell screening,ten monoclonal antibodies(mAbs)against gliadin and after optimization were obtained,a pair of mAbs suitable for its detection(capture antibody:mAb 7;detection antibody:HRP-labeled mAb 6)were *** on two of these antibodies,a sandwich ELISA with limit of detection(LOD)of 60 ng/mL in negative milk,and more importantly,negligible cross-reactivity to other allergens was *** average recoveries for gliadin in negative milk were 99.16%-100.07%using the sandwich *** also developed LFIA strips for the rapid detection of gliadin with visual limit of detection(vLOD)of 25 ng/mL and calculated LOD value of 6.56 ng/mL in negative *** test results of positive samples obtained from the LFIA strips were highly consistent with those of the sandwich ***,the developed LFIA strip is an effective and reliable tool for the rapid and on-site detection of wheat allergen in milk.

关键词： Wheat allergen Monoclonal antibody Sandwich ELISA LFIA Food allergen Milk

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Metabolic profile of chiral cobalt oxide nanoparticles in vitro and in vivo

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Nano Research 2021年第7期14卷 2451-2455页

作者： Si Li Liwei xu Meiru Lu Maozhong Sun liguang xu Changlong Hao Xiaoling Wu Chuanlai xu Hua Kuang International Joint Research Laboratory for Biointerface and Biodetection Jiangnan UniversityWuxi 214122China State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China

In this study,we investigated the in vivo behaviors of chiral cobalt oxide nanoparticles(Co_(3)O_(4) NPs)stabilized with D-or L-cysteine(denoted D-or L-NPs,respectively),as representative chiral metal oxide *** Co_(3)O_(4) NPs exerted no observable cytotoxicity within the tested dose *** D-NPs and L-NPs were internalized by dendritic cells through caveola-dependent endocytosis,and L-NPs entered the cells faster than ***,a metabolic analysis indicated that chiral L-NPs had the same effect on the level of bile acids in the liver as D-NPs,which is attributed to the almost equal amount of farnesoid X receptor(FXR)induced by the chiral *** study suggests that low chiroptical activity nanomaterials would not affect the metabolism and kinetics under physiological conditions even if there is some difference in their transport.

关键词： chiral metal oxide endocytosis bile acid metabolism

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