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Science China Chemistry 2015年第5期58卷 810-818页

作者： Qiumei Feng Hongyuan Chen jingjuan xu State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University

A novel disposable paper-based bipolar electrode （BPE） array is fabricated for multiplexed electrochemiluminescence （ECL） detection of pathogenic DNAs. This proposed BPE array device consists of 15 units, each consisting of six sensing cells and two reporting cells patterned using hydrophobic wax. A hairpin structure DNA assembled on the cathodes of BPEs hybridizes with Pt nanoparticles （NPs） labeled probe DNA in the presence of complementary target DNA. The introduction of Pt NPs catalyzes the reduction of dissolved 02 at cathodes and induces an enhanced ECL signal from Ru（bpy）32＋/tripropylamine （TPrA） at the anodes of BPEs. The dissolved 02 lost in reduction reaction could be promptly replenished due to the relatively large contact area of the paper-based cells with air, which ensures the stability of ECL signal. This obtained paper-based BPE array sensor showed excellent performances for the multiplexed analysis of the syphilis （Treponema pallidum） gene, the immunodeficiency virus gene （HIV） and hepatitis B virus gene （HBV）.

关键词： paper-based device bipolar electrode array pathogenic DNAs multiple detection electrochemiluminescence

Progress in the studies of photoelectrochemical sensors

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Science China Chemistry 2009年第11期52卷 1789-1800页

作者： WANG GuangLi xu jingjuan CHEN HongYuan Key Lab of Analytical Chemistry for Life Science Nanjing UniversityNanjing 210093China

Photoelectrochemical sensor is a new kind of developing analytical device based on the photoelectrochemical properties of *** of its remarkable sensitivity,inherent miniaturization,portability and easy integration,photoelectrochemical analysis is becoming a promising analytical *** review focuses on the basic principles,classification,characteristics,and research progress of photoelectrochemical sensors with 94 *** prospect of the development of photoelectrochemical sensors is also evaluated and discussed.

关键词： photoelectrochemistry sensor review

Mass transport in nanofluidic devices

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Science China Chemistry 2012年第4期55卷 453-468页

作者： WANG Chen xu jingjuan CHEN HongYuan XIA XingHua State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210093China Department of Physical Chemistry China Pharmaceutical UniversityNanjing 210009China

Nanofluidics is a recent appearing research field, introduced in 1995 as an analogue of the field of microfluidics, and has been becoming popular in the past few years. The proximity of the channel dimension, the Debye length, and the size of biomolecules such as DNA and proteins gives the unique features of nanofluidic devices. Of various unique properties of the nanofluidics, mass transport in nanochannel plays determining roles in fundamental reaches and practical applications of nanofluidic device. Thus, much work including numerical and experimental researches has been performed to investigate the mass transport behaviors in nanofluidic devices. This review summarizes the fabrication technologies for nanofluidic devices, the mass transport behaviors in nanochannel, and their applications in bioanalysis. The main focus will be laid on the effects of nanochannel size and surface charge on mass transport including electrokinetic transport of charged analytes, diffusion of electric neutral molecules, ionic current rectification, concentration polarization, nonlinear electrokinetic flow at the micro-nanofluidic interfaces.

关键词： nanofluidics mass transport fabrication ionic current rectification microfluidics concentration polarization nonlinear electrokinetic flow bioanalysis

不同雾化时机对无创通气治疗的慢性阻塞性肺疾病急性加重患者的效果比较

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中国实用护理杂志 2024年第1期40卷 20-26页

作者：徐静娟杨燕刘艳姚莉万文霞凌楠常州市第一人民医院门诊办公室常州213000 常州市第一人民医院呼吸与危重症医学科常州213000

目的对比无创间歇期雾化与无创同时雾化对慢性阻塞性肺疾病急性加重患者雾化过程中经皮二氧化碳分压(PtCO_(2))动态变化及治疗效果的影响。方法采用随机平行对照试验方法,便利抽样选取2021年10月至2022年9月常州市第一人民医院收治慢性... 详细信息

目的对比无创间歇期雾化与无创同时雾化对慢性阻塞性肺疾病急性加重患者雾化过程中经皮二氧化碳分压(PtCO_(2))动态变化及治疗效果的影响。方法采用随机平行对照试验方法,便利抽样选取2021年10月至2022年9月常州市第一人民医院收治慢性阻塞性肺疾病急性加重患者70例,根据随机数字表法分为对照组和试验组各35例,对照组给予无创间歇期雾化吸入,试验组给予无创同时雾化吸入,观察2组雾化0、5、10、15 min(雾化结束点)的PtCO_(2)数值,记录每日的动脉血气分析指标(主要包括PaCO_(2)、PaO_(2)、pH),记录治疗前、治疗第4天、治疗第7天临床肺部感染评分和慢性阻塞性肺疾病患者自我评估评分。结果最终纳入对照组和试验组各33例患者。对照组男25例,女8例,年龄(75.33±8.24)岁;试验组男25例,女8例,年龄(72.39±8.56)岁。对照组患者雾化0、5、10、15 min PtCO_(2)数值分别为(63.83±12.47)、(64.40±12.57)、(65.42±13.77)、(66.62±14.59)mmHg(1 mmHg=0.133 kPa),各时间点PtCO_(2)之间总体上差异有统计学意义(F=8.05,P0.05);2组患者PaCO_(2)和pH值均随治疗时间好转,但对照组最早在治疗第4天开始与治疗前比较差异有统计学意义(P<0.05),而试验组在治疗第2天开始与治疗前比较差异有统计学意义(P<0.05)。结论2种雾化方式均取得良好的治疗效果,但无创同时雾化在雾化过程中更能维持PtCO_(2)的平稳,安全性更高,且能够更早改善患者动脉血气指标PaCO_(2)和pH值,是无创通气与雾化治疗联合运用尤其是患者伴有高碳酸血症时更宜选择的雾化方式。

关键词：肺疾病,慢性阻塞性无创机械通气雾化吸入经皮二氧化碳分压

A High Spatiotemporal Iontronic Single-Cell Viscometer

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Research 2022年第4期2022卷 239-247页

作者： Tianyang Zhang Siyuan Yu Bing Wang Yitong xu Xiaomei Shi Weiwei Zhao Dechen Jiang Hongyuan Chen jingjuan xu State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical EngineeringNanjing UniversityNanjing210023China

Ideal single-cell viscometer has remained unachieved,leaving a gap in current palette of single-cell *** of single-cell viscosity could contribute to our knowledge of fundamental biological processes,e.g.,mass diffusion,biochemical interaction,and cellular responses to many diseases and *** advances have been made to this end,existing methods generally suffer from limitations,e.g.,low spatiotemporal ***,we describe a high spatiotemporal iontronic single-cell viscometer that operates upon a patch clamp integrated with double-barreled nanopores separated by a septum of ca.32 *** system enables reversible electroosmotic manipulation of the adjacent small fluid bridging two nanopores,the viscous alternation of which could be sensitively monitored by the ionic *** practical cellular studies,significantly,our findings reveal not only the less deviated medium viscosities than those of lysosomes and mitochondria but also the highest viscosities in the near-nuclear region than those of mitochondrion-dense and lysosome-dense *** work has provided an accessible single-cell viscometer and enriched the armory of single-cell nanotools.

关键词： pores viscosity bridging

基于科学引文数据库的"严肃游戏"在医学教育领域中的研究热点与趋势分析

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中华医学教育杂志 2024年第9期44卷 677-682页

作者：苏小平徐静娟梁永春吴智水青海省人民医院重症医学科西宁810000 苏州大学附属第三医院医疗行风监督处常州213000 无锡太湖学院健康与护理学院无锡214000 苏州大学附属第三医院脊柱外科常州213000

目的分析"严肃游戏"在医学教育领域的研究热点与趋势。方法检索2004年1月1日至2023年9月20日科学引文数据库核心合集,运用CiteSpace软件对该领域文献的分布特征、研究热点和趋势进行分析。结果共筛选出符合检索条件的文献1 026篇,"严肃... 详细信息

目的分析"严肃游戏"在医学教育领域的研究热点与趋势。方法检索2004年1月1日至2023年9月20日科学引文数据库核心合集,运用CiteSpace软件对该领域文献的分布特征、研究热点和趋势进行分析。结果共筛选出符合检索条件的文献1 026篇,"严肃游戏"在医学教育领域研究的发文量总体呈上升趋势;排在前5位的关键词为医学教育(161次)、"严肃游戏"(136次)、虚拟现实(86次)、医学生(76次)、表现(75次);关键词聚类前5位是医学教育、人机交互、激励机制、游戏化、虚拟现实;2020年1月1日至2023年9月20日的突现关键词为游戏策略、游戏设计、技术、管理、游戏化、游戏理论。结论 "严肃游戏"在医学教育领域的研究日益受到重视,人机交互、游戏化、虚拟现实为研究热点;游戏理论和游戏策略、游戏设计为该领域的研究趋势,研究热点和研究趋势表现为多样化特征,未来还有待进一步探索。

关键词：教育,医学 "严肃游戏" 应用现状可视化热点趋势

Magnetic particles and cadmium sulfide nanoparticles tagging for signal-amplifying detection of nucleic acids

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Science China Chemistry 2011年第8期54卷 1304-1310页

作者： DONG XiaoYa, ZHAO WeiWei, xu jingjuan & CHEN HongYuan State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210093, China 1. State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Nanjing 210093 China

A versatile DNA recognition system using cadmium sulfide nanoparticles (CdS NPs) as labels was proposed for ultrasensitive detection of specific sequence DNA based on target recycling. This strategy utilized the magnetic particles (MNPs) for the immobilization of linker DNA and CdS NPs and the subsequent target DNA hybridization. Using the unique characteristic of nicking endonuclease for cutting one specific strand of double strand DNA (ds DNA), the linker DNA could be transected and the target DNA could be liberated for re-hybridization and hence the amount of released CdS NPs was enhanced. Due to the advantage of the MNPs and signal amplification from the target recycling, the analyte DNA could be detected by the square-wave stripping voltammetry (SWV) in a wide linear range from 0.4 fM to 100 fM with the detection limit down to 0.08 fM. The proposed DNA detection strategy possesses high sensitivity, satisfactory reproducibility and excellent stability, which might have potential in other DNA biological assays.

关键词： CdS nanoparticles square wave stripping voltammetry nicking endonuclease electrochemical DNA assay

Lingguizhugan Decoction, a Chinese herbal formula, improves insulin resistance in overweight/obese subjects with non-alcoholic fatty liver disease: a translational approach

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Frontiers of Medicine 2022年第5期16卷 745-759页

作者： Liang Dai jingjuan xu Baocheng Liu Yanqi Dang Ruirui Wang Lijie Zhuang Dong Li Lulu Jiao Jianying Wang Lei Zhang Linda LDZhong Wenjun Zhou Guang Ji Institute of Digestive Diseases Longhua HospitalShanghai University of Traditional Chinese MedicineShanghai 200032China Clinical Research Academy Peking University Shenzhen HospitalShenzhen 518032China Department of Integrated Traditional and Western Medicine Jinling HospitalMedical School of Nanjing UniversityNanjing 210002China Shanghai Innovation Centre of TCM Health Service Shanghai University of Traditional Chinese MedicineShanghai 201203China Sanlin Health Centre of Pudong New District Shanghai 200120China Zhangjiang Health Centre of Pudong New District Shanghai 201203China Beicai Health Centre of Pudong New District Shanghai 201204China Hong Kong Chinese Medicine Study Centre Hong Kong Baptist UniversityHong Kong 999077China

Lingguizhugan Decoction(LGZG)has been investigated in basic studies,with satisfactory effects on insulin resistance in non-alcoholic fatty liver disease(NAFLD).This translational approach aimed to explore the effect and underlying mechanism of LGZG in clinical setting.A randomized,double-blinded,placebo-controlled trial was performed.A total of 243 eligible participants with NAFLD were equally allocated to receive LGZG(two groups:standard dose and low dose)or placebo for 12 weeks on the basis of lifestyle *** primary efficacy variable was homeostasis model assessment of insulin resistance(HOMA-IR).Analyses were performed in two populations in accordance with body mass index(BMI;overweight/obese,BMI 24 kg/m^(2);lean,BMI<24 kg/m^(2)).For overweight/obese participants,low-dose LGZG significantly decreased their HOMA-IR level compared with placebo(0.19(1.47)versus 0.08(1.99),P=0.038).For lean subjects,neither dose of LGZG showed a superior effect compared with *** DNA immunoprecipitation sequencing and real-time qPCR found that the DNA N6-methyladenine modification levels of protein phosphatase 1 regulatory subunit 3A(PPP1R3A)and autophagy related 3(ATG3)significantly increased after LGZG intervention in overweight/obese ***-dose LGZG effectively improved insulin resistance in overweight/obese subjects with *** underlying mechanism may be related to the regulation of DNA N6-methyladenine modification of PPP1R3A and *** subjects may not be a targeted population for LGZG.

关键词： insulin resistance non-alcoholic fatty liver disease Chinese herbal medicine randomized controlled trial DNA N6-methyladenine modification

Mechanism of Guangdong Shenqu in regulating intestinal flora in mice with food stagnation and internal heat based on 16S rDNA sequencing Author links open overlay panel

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Journal of Traditional Chinese Medical Sciences 2024年第2期11卷 232-244页

作者： Yani Jiang Zhenhong Zhang Han Chen Yanmin Wang Yanrui xu Zijie Chen Yan xu jingjuan Wang School of Chinese Materia Medica Beijing University of Chinese MedicineBeijing102488China School of Chinese Traditional Medicine Beijing University of Chinese MedicineBeijing102488China Department of Chemistry Cleveland State UniversityCleveland44115USA

Objective To investigate the effect of Guangdong Shenqu(GSQ)on intestinal flora structure in mice with food stagnation through 16S rDNA *** Mice were randomly assigned to control,model,GSQ low-dose(GSQL),GSQ medium-dose(GSQM),GSQ high-dose(GSQH),and lacidophilin tablets(LAB)groups,with each group containing 10 mice.A food stagnation and internal heat mouse model was established through intragastric administration of a mixture of beeswax and olive oil(1:15).The control group was administered normal saline,and the model group was administered beeswax and olive oil to maintain a *** GSQL(2 g/kg),GSQM(4 g/kg),GSQH(8 g/kg),and LAB groups(0.625 g/kg)were administered corresponding drugs for 5 *** administration,16S rDNA sequencing was performed to assess gut microbiota in mouse fecal *** The model group exhibited significant intestinal flora *** GSQ administration,the abundance and diversity index of the intestinal flora increased significantly,the number of bacterial species was regulated,andαandβdiversity were *** administration increased the abundance of probiotics,including Clostridia,Lachnospirales,and Lactobacillus,whereas the abundance of conditional pathogenic bacteria,such as Allobaculum,Erysipelotrichaceae,and Bacteroides *** prediction analysis indicated that the pathogenesis of food stagnation and GSQ intervention were primarily associated with carbohydrate,lipid,and amino acid metabolism,among other metabolic *** The digestive mechanism of GSQ may be attributed to its role in restoring diversity and abundance within the intestinal flora,thereby improving the composition and structure of the intestinal flora in mice and subsequently influencing the regulation of metabolic pathways.

关键词： Guangdong Shenqu Food stagnation Gut microbiota High-throughput gene sequencing Species annotation Metabolic pathway

裙带菜(Undaria pinnatifida)配子体培养条件的优化

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海洋湖沼通报 2017年第5期 102-108页

作者：徐群刘岩徐静娟中国海洋大学藻类学与藻类养殖研究室山东青岛266003

以日本三陆地区的裙带菜(Undaria pinnatifida)配子体为实验材料,采用双因素完全交叉分组实验,研究了温度(18℃、21℃、24℃、27℃)、光照强度(50μE·m^(-1)·s^(-1)、80μE·m^(-1)·s^(-1)、110μE·m^(-1)·s^(-1)、140μE·m^(-1)·s^(-1))、培养方式(静置培养、磁力搅拌培养、充气悬浮培养)、接种密度(0.1g/L、0.2g/L、0.4g/L、0.6g/L、0.8g/L、1.0g/L)对裙带菜配子体生长的影响。结果显示,光强和温度等环境因素以及两者的交互作用对配子体生长有较大影响。21℃、50μE·m^(-1)·s^(-1)的条件组合是实验中配子体培养的最佳条件。在培养的第5d到第10d之间,裙带菜配子体克隆的细胞增长率最大,可在第10d对配子体进行继代培养,使其保持较高的增长率。培养方式和初始接种密度以及两者的交互作用对配子体生长有较大影响,初始接种密度为0.1~0.2g/L的充气培养条件是配子体培养的最佳条件组合。本实验得出,日本三陆裙带菜雌配子体克隆扩培的最适培养条件为:温度21℃、光强50μE·m^(-1)·s^(-1)、接种密度0.1~0.2g/L,充气悬浮培养。

关键词：裙带菜配子体培养条件