A High Spatiotemporal Iontronic Single-Cell Viscometer
作者机构：State Key Laboratory of Analytical Chemistry for Life ScienceSchool of Chemistry and Chemical EngineeringNanjing UniversityNanjing210023China
出 版 物：《Research》 (研究（英文）)
年 卷 期：2022年第2022卷第4期
页 面：239-247页
核心收录：
学科分类：080704[工学-流体机械及工程] 080103[工学-流体力学] 08[工学] 0807[工学-动力工程及工程热物理] 0801[工学-力学（可授工学、理学学位）]
基 金：We thank the National Natural Science Foundation of China(Grant Nos.21974059,22034003,and 22174063) Excellent Research Program of Nanjing University(ZYJH004)
摘 要：Ideal single-cell viscometer has remained unachieved,leaving a gap in current palette of single-cell *** of single-cell viscosity could contribute to our knowledge of fundamental biological processes,e.g.,mass diffusion,biochemical interaction,and cellular responses to many diseases and *** advances have been made to this end,existing methods generally suffer from limitations,e.g.,low spatiotemporal ***,we describe a high spatiotemporal iontronic single-cell viscometer that operates upon a patch clamp integrated with double-barreled nanopores separated by a septum of ca.32 *** system enables reversible electroosmotic manipulation of the adjacent small fluid bridging two nanopores,the viscous alternation of which could be sensitively monitored by the ionic *** practical cellular studies,significantly,our findings reveal not only the less deviated medium viscosities than those of lysosomes and mitochondria but also the highest viscosities in the near-nuclear region than those of mitochondrion-dense and lysosome-dense *** work has provided an accessible single-cell viscometer and enriched the armory of single-cell nanotools.
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