检索结果-南通市图书馆

Mutations in FgPrp6 suppressive to the Fgprp4 mutant in Fusarium graminearum

Journal of Integrative Agriculture 2022年第5期21卷 1375-1388页

作者： LI Chao-hui FAN Zhi-li HUANG Xin-yi WANG Qin-hu JIANG Cong xu jin-rong jin Qiao-jun NWAFU-PU Joint Research Center/State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F UniversityYang ling 712100P.R.China Institute of Plant Protection/Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology Jiangsu Academy of Agricultural SciencesNanjing 210014P.R.China Department of Botany and Plant Pathology Purdue UniversityWest LafayetteIN47907USA

The pre-mRNA processing factor Prp6 is an essential component of the U4/U6.U5 tri-small nuclear ribonucleoprotein(snRNP).In a previous study,mutations were identified in the PRP6 ortholog in four suppressors of Fgprp4 that was deleted of the only kinase FgPrp4 among the spliceosome components in the plant pathogenic fungus Fusarium *** this study,we identified additional suppressor mutations in FgPrp6 and determined the suppressive effects of selected *** total,12 mutations of FgPRP6 were identified in 20 suppressors of Fgprp4 by sequencing *** three mutation sites are in the linker region of FgPrp6,seven are in the first two HAT ***-seq analysis showed that suppressor mutations on different sites caused different splicing efficiency *** suppressive effects of E308K and R230H were *** to human and fission yeast,the FgPrp6 was phosphorylated by the FgPrp4 ***,the conserved Prp4-phosphorylation sites T261,T219&T221,and predicted phosphorylation sites T199&T200 on FgPrp6 were dispensable for the function of FgPrp6 in hyphal growth and sexual reproduction but important in plant *** are required for the infectious growth of *** in wheat ***-seq analysis of the wheat lemma infected with Fgprp6/FgPRP6^(Δ199-221)-GFP or Fgprp6/FgPRP6^(Δ250-262)-GFP showed that 28 and 35% introns had splicing defects,respectively,which may be responsible for their defects in plant infection.

关键词： RNA splicing FgPrp6 suppressor plant infection phosphorylation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

FgGap1 localizes to the cytoplasm and septal pore and plays a role in vegetative growth,sexual reproduction,and pathogenicity in Fusarium graminearum

FgGap1 localizes to the cytoplasm and septal pore and plays ...

引用

中国植物病理学会2015年学术年会

作者： CHEN Dai-peng LIU Hui-quan xu jin-rong Purdue-NWAFU Joint Research Center and State Key Laboratory of Crop Stress Biology for Arid Areas College of Plant ProtectionNorthwest A&F University

Fusarium head blight（FHB） caused by *** is a devastating disease of wheat,barley,and other grain cereals *** not only causes severe yield losses but also contaminates harmful mycotoxins in infested ***1,a gene identified by mimp1/impala transposon-tagging system,encoded a growth associate protein in *** homologous gene in budding yeast is not ***,the insertion mutant of FgGap1 is pleiotropic defective in *** further characterization,we generated its deletion mutant by split marker approach and assayed its biological phenotypes in this *** deletion mutant ΔFggap1 formed a small size and few aerial hyphae colony,produced limit conidia and could only germinated shorter germtube,wavy *** mutant produced small size and less perithecium contained aborted ascogenous *** infection assays with flowering wheat heads,the virulence reduced at least 80% and the production of DON reduced 50%compared with wild *** complementation assays,the reintroduction of the FgGap1-eGFP rescued the defects in the mutant and GFP signals located to cytoplasm and septal *** previous study,the subcellular localization of FgKIN1 was also in septal pore and Fgkinl regulated the localization of *** predicted that FgGAP1 protein may regulate the localization of microtube in septal pore.

关键词： Conidia germination Septal pore Perithecium

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

改性纳米SiOx复合涂膜在莲藕保鲜中应用

引用

食品工业 2015年第3期36卷 150-154页

作者：许金蓉广西科技大学生物与化学工程学院柳州545006

以壳聚糖、海藻酸钠、改性纳米SiOx为涂膜材料,按照L9(34)正交试验制备不同配比的改性纳米SiOx复合涂膜。结果表明,复合保鲜膜的最佳配比为1.50%壳聚糖、0.60%海藻酸钠、0.02%改性纳米SiOx时可有效保持莲藕水分,抑制莲藕褐变,提高贮藏... 详细信息

以壳聚糖、海藻酸钠、改性纳米SiOx为涂膜材料,按照L9(34)正交试验制备不同配比的改性纳米SiOx复合涂膜。结果表明,复合保鲜膜的最佳配比为1.50%壳聚糖、0.60%海藻酸钠、0.02%改性纳米SiOx时可有效保持莲藕水分,抑制莲藕褐变,提高贮藏期间产品品质,延长货架期。

关键词：莲藕壳聚糖海藻酸钠改性纳米SiOx 保鲜

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

引用

Chinese Medical Journal 2008年第12期121卷 1080-1084页

作者： TIAN Heng-li CHEN Shi-wen xu Tao HU jin rong Bo-ying WANG Gan GAO Wen-wei CHEN Hao Department of Neurosurgery Shanghai Sixth People's Hospital Shanghai Jiao Tong University Shanghai 200233 China

Background Acute subdural haematoma （ASDH） is a common traumatic brain injury with a relatively high mortality rate. However, few studies have examined the factors predicting the outcome of isolated traumatic ASDH. This clinical study examined the hospital mortality and analyzed the risk factors for mortality in patients treated surgically for isolated traumatic ASDH.Methods We collected 308 consecutive patients who underwent neurosurgery for isolated traumatic ASDH between January 1999 and December 2007 and used multivariate Logistic regression analysis to evaluate the influence of 11 clinical variables on hospital mortality.Results The overall hospital mortality was 21.75% （67/308）. Age （OR=1.807）, preoperative Glasgow Coma Score （OR=0.316）, brain herniation （OR=2.181） and the time from trauma to decompression （OR=1.815） were independent predictors of death, while no independent association was observed between hospital mortality and haematoma volume, midline shift, acute brain swelling or brain herniation duration, although these variables were correlated with hospital mortality in univariate analyses.Conclusions This study identified the risk factors for hospital mortality in patients who underwent surgical treatment for isolated traumatic ASDH. An increased risk of death occurs in patients who are over 50 years of age and have lower preoperative Glasgow Coma Scores, the presence of brain herniation and a long interval between trauma and decompression. The findings should help clinicians determine management criteria and improve survival.

关键词： acute subdural haematoma hospital mortality risk factors

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The Prp4 kinase regulates splicing efficiency and the interaction of tri-snRNP components in Fusarium graminearum

The Prp4 kinase regulates splicing efficiency and the intera...

引用

中国植物病理学会2015年学术年会

作者： GAO xu-li jin Qiao-jun xu Qi-jun LI Chao-hui LIU Hui-quan WANG Chen-fang xu jin-rong State Key Laboratory of Crop Stress Biology for Arid Areas Northwest A& F University

Pre-mRNA splicing is an important gene expression regulation mechanism in eukaryotic *** PRPA gene encode the only protein kinase involved in pre-mRNA *** the fission yeast and mammals,it is an essential gene but the budding yeast lacks a distince Prp4 ortholog which has limited number of small *** a previous study of systematic characterization of protein kinases in the wheat scab fungus Fusarium graminearum,we found that the FgprpA mutant was viable although it was significantly reduced in growth ***-seq analysis showed that deletion of FgPRPA did not block intron splicing but affected the splicing efficiency for 90% of the introns,including genes important for DNA ***,the Fgprp4 mutant was unstable and we have isolated a total of 49 spontaneous fast-growing *** in the PRP6,PRP31,BRR2,and PRP8 orthologs were identified in nine suppressor *** mutations in Prp6 and Prp31 orthologs have not been previously reported in their *** FgPrp31,the non-sense mutation at R464 resulted in the truncation of the C-terminal 130 aa region containing all the Prp4-phosphorylation *** further showed that this C-terminal tail of FgPrp31 displayed self-inhibitory interaction with its N-terminal region in yeast two-hybrid and co-IP ***,our results showed that FgPrp4 is critical for splicing efficiency but not essential for intron *** mutations identified in this study must occur in the interface of Prp6,Prp8,Prp31,and Brr2 in the tri-snRNP and affect B-complex *** of Prp31,and possibly Prp6,by Prp4 may release its self-inhibitory binding and enables its interaction with Prp8 and Brr2.

关键词： Prp4 kinase pre-mRNA splicing splicing efficiency tri-snRNP

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Recent Advances in Artificial Sensory Neurons:Biological Fundamentals,Devices,Applications,and Challenges

引用

Nano-Micro Letters 2025年第3期17卷 168-216页

作者： Shuai Zhong Lirou Su Mingkun xu Desmond Loke Bin Yu Yishu Zhang rong Zhao Guangdong Institute of Intelligence Science and Technology HengqinZhuhai 519031People’s Republic of China College of Integrated Circuits Zhejiang UniversityHangzhou 3112000People’s Republic of China ZJU-Hangzhou Global Scientific and Technological Innovation Center Hangzhou 310027People’s Republic of China Department of Science Mathematics and TechnologySingapore University of Technology and DesignSingapore 487372Singapore Department of Precision Instruments Tsinghua UniversityBeijing 100084People’s Republic of China Center for Brain‑Inspired Computing Research Tsinghua UniversityBeijing 100084People’s Republic of China IDG/McGovern Institute for Brain Research Tsinghua UniversityBeijing 100084People’s Republic of China

Spike-based neural networks,which use spikes or action potentialsto represent information,have gained a lot of attention because of their high energyefficiency and low power *** fully leverage its advantages,convertingthe external analog signals to spikes is an essential *** including analog-to-digital converters or ring oscillators,and sensorssuffer from high power and area *** efforts are devoted to constructingartificial sensory neurons based on emerging devices inspired by the biologicalsensory *** can simultaneously perform sensing and spike conversion,overcoming the deficiencies of traditional sensory *** review summarizesand benchmarks the recent progress of artificial sensory *** starts with thepresentation of various mechanisms of biological signal transduction,followed bythe systematic introduction of the emerging devices employed for artificial ***,the implementations with different perceptual capabilitiesare briefly outlined and the key metrics and potential applications are also ***,we highlight the challenges and perspectives for the future development of artificial sensory neurons.

关键词： Artificial intelligence Emerging devices Artificial sensory neurons Spiking neural networks Neuromorphic sensing

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Two genes,SP307 and SP970,are required for sexual development in the ascomycete fungus Fusarium graminearum

Two genes,SP307 and SP970,are required for sexual developmen...

引用

中国植物病理学会2015年学术年会

作者： SUN Pan-pan WU Zhong-shou WANG Chen-fang xu jin-rong State Key Laboratory of Crop Stress Biology for Arid Areas Northwestern A&F University

The lamentous ascomycete Fusarium graminearum is one of the causal agents of Fusarium head blight（FHB） of wheat and *** many other plant pathogenic fungi,*** uses ascospores as the primary inoculum to infect wheat or barley *** development involves complex cellular processes and is under polygenic *** this study,two genes,SP307 and SP970,were found to be not essential for growth and pathogenesis but important for sexual reproduction in ***307 deletion mutants produced normal-shaped perithecia,and a ascus can only form 0-2 *** sexual induction,the SP307 deletion mutant rarely formed cirrhi that had limited extension in comparison with those of the wild-type,and SP307 deletion mutants can discharge a small amount of ***970 deletion mutants produced normal-shaped perithecia and ascospores,yet ascospores were observed to precociously germinate inside the *** days after sexual induction,mature perithecia forcibly discharge ascospores,and cirrhi of ascospores remain at the tip of *** contrast,SP970 deletion mutants did not discharge ascospores and did not form any observable ascospore *** results indicate that SP307 and SP970,are required for sexual reproduction in the ascomycete fungus Fusarium graminearum.

关键词： Fusarium graminearum sexual development ascospore

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

MoFRQ plays a critical role in conidium development,appressorium formation,and pathogenicity in Magnaporthe oryzae

MoFRQ plays a critical role in conidium development,appresso...

引用

中国植物病理学会2015年学术年会

作者： ZHANG Qiang GAO xu-li ZHANG Shi-jie JIANG Cong WANG Chen-fang xu jin-rong Purdue-NWAFU Joint Research Center State Key Laboratory of Crop Stress Biology for Arid AreasCollege of Plant ProtectionNorthwestern A&F University

Circadian rhythm organizes inner physiology with respect to the external world,providing life with the ability to anticipate and thereby better prepare for major fluctuations in its *** products of the FREQUENCY（FRQ） gene are central components of the Neurospora crassa *** determine its function in Magnaporthe oryzae,the causal agent of rice blast,we functionally characterized the MoFRQ gene that is orthologous to FRQ of *** Mofrq mutant had no obvious defect in vegetative *** colony morphology of ΔMofrq mutant was similar with the wildtype at constant darkness,but was significantly reduced in aerial hyphae at constant *** also was radically reduced in conidiation,conidium germination and appressorium formation on hydrophobic *** of MoFRQ also impacted plant infection and *** mobilization of glycogen and lipid droplets during appressorium formation were retarded in the AMofrq *** transcription level of MoFRQ was up-regulation under light conditions compared with that of in the *** with light increased the fluorescence intensity in the nucleus and cytoplasm in hypha of Mofrq-R（MoFRQ-GFP）.cAMP-dependent PKA activities were reduced in the Mofrq ***,Site-directed mutagenesis confirmed that the cysteine site of 663-aa but not 492-aa was essential for the function of *** results indicate that the MoFRQ gene is important to conidiation,conidial germination,appressorium formation,and pathogenicity in ***.

关键词： circadian rhythm rice blast morphogenesis

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

基于模式比较的遗传算法

引用

计算机应用 2008年第2期28卷 275-278页

作者：徐金荣李允西南交通大学信息科学与技术学院成都610031

在分析了模式阶、模式定义长度和种群多样性三者之间关系的基础上,提出一种新的基于模式比较的遗传算法。该算法的核心在于,使用配对算子来提高子代种群的多样性。配对算子不像简单遗传算法那样随机选择配对,而是根据模式阶和模式长度... 详细信息

在分析了模式阶、模式定义长度和种群多样性三者之间关系的基础上,提出一种新的基于模式比较的遗传算法。该算法的核心在于,使用配对算子来提高子代种群的多样性。配对算子不像简单遗传算法那样随机选择配对,而是根据模式阶和模式长度对染色体进行配对。实验结果表明,该算法有效地避免了简单遗传算法中因种群多样性较差而造成的早熟现象,显著地提高了收敛速度和搜索全局最优解的能力。

关键词：模式遗传算法配对算子种群多样性

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于PSO和SVM的发酵过程建模与优化控制

引用

微计算机信息 2008年第19期24卷 31-32,39页

作者：徐进荣潘丰江南大学通信与控制工程学院 214122

针对微生物发酵过程的建模与优化控制问题,利用支持向量机理论进行发酵过程的建模,并提出采用粒子群优化算法对支持向量机建模过程中的重要参数进行优化调整。仿真结果表明参数优化调整后得到的模型能取得更好的预测效果。同时基于此模... 详细信息

针对微生物发酵过程的建模与优化控制问题,利用支持向量机理论进行发酵过程的建模,并提出采用粒子群优化算法对支持向量机建模过程中的重要参数进行优化调整。仿真结果表明参数优化调整后得到的模型能取得更好的预测效果。同时基于此模型,在补料分批发酵控制过程中采用粒子群优化算法对补料速率进行优化控制,仿真结果表明,该方法能够提高最终的产物合成量。

关键词：支持向量机粒子群参数选择发酵过程补料

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：