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Lentivirus-mediated knockdown of phosphodiesterase 4D improved depression-like behaviors in mice

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中国药理学与毒理学杂志 2016年第10期30卷 1020-1021页

作者： ZHANG Cong CHENG Yu-fang NIU Bo xu jiang-ping Guangdong Provincial Key Laboratory of New Drug Screening School of Pharmaceutical SciencesSouthern Medical University

OBJECTIVE To examine whether long-form phosphodiesterase-4(PDE4)knockdown by lentiviral RNA construct containing a speci fi c micro RNA/mi RNAmir hairpin structure reversed depression-like symptoms caused by chronic unpredictable mild stress(CUMS)in *** In this research,the study was performed on adult male C57 mice,weighing(25±5)g,kept in a controlled *** animal model was used recapitulate a multiple of behavioral characteristics and biochemical states of depression in *** forced swimming test(FST)and the tail suspension test(TST)were used to detectthe state of *** blotting analysis was used to assess protein levels of c AMP response element binding protein(CREB,unphosphorylated and phosphorylated[p CREB])to explore the neurochemical *** CUMS decreased c AMP levels(P<0.01)and produced depression-like symptoms in FST(P<0.01)and TST(P<0.01).Microinfusions of lentiviruses reversed CUMS-induced c AMP decline(P<0.05)and depression-like ***,CUMS caused a significant reduction in protein kinase A and CREB phosphorylation,and brain-derived neurotrophic factor transcription,both of which were partially attenuated by lentivirus-mediated knockdown of ***,the phosphorylation of extracellular signal-regulated kinase 1/2 was reduced in CUMS-exposed mice,which was reversed by 4Dmi RNA *** together,this study demonstrated that PDE4Dmi RNA improved the CUMS-induced depressionlike symptoms that might be related to the increase in hippocampal c AMP and p CREB *** Hence,PDE4D inhibitors can serve as potential antidepressants,and their antidepressant activity is partially mediated by the activation of c AMP signaling pathway in the *** other words,long-form PDE4D knockdown may offer a promising treatment for major depression disorder.

关键词： antidepressant CREB long-form PDE4D major depression disorder

Constructing cell-membrane-mimic grain boundaries for high-performance n-type Ag_(2)Se using high-dielectric-constant TiO_(2)

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Journal of Materials Science & Technology 2024年第12期179卷 138-144页

作者： Yi-Yan Liao Qiang Sun xu-ping jiang Hao Wu Bang-Zhou Tian Ze-Gao Wang Kun Zheng Lei Yang School of Materials Science&Engineering Sichuan UniversityChengdu 610064China State Key Laboratory of Oral Diseases National Clinical Research Center for Oral DiseasesWest China Hospital of StomatologySichuan UniversityChengdu 610041China Sichuan Provincial Engineering Research Center of Oral Biomaterials Sichuan UniversityChengdu 610041China Department of Stomatology the First Medical CentreChinese PLA General HospitalBeijing 100853China Beijing Key Lab of Microstructure and Properties of Solids Faculty of Materials and ManufacturingBeijing University of TechnologyBeijing 100124China

The coupling of charge carrier and phonon transport limits the application of Ag_(2)Se as a low-toxic near-room-temperature thermoelectric *** that reduce the thermal conductivity via enhanc-ing the phonon scattering usually lead to reduced carrier mobility due to high grain boundary potential *** this study,we developed a cell-membrane-mimic grain boundary engineering strategy for de-coupling the charge carrier and phonon scattering through decorating high-dielectric-constant rutile TiO_(2) at Ag_(2)Se grain boundaries to enable the charge carrier/phonon selective *** nano-sized TiO_(2) with high dielectric permittivity can secure the charge carrier transport by shielding the interfacial Coulomb potential to lower the energy barrier of grain boundaries,rendering an enhanced power ***,benefited from the enhanced phonon scattering by TiO_(2) nanoparticles,a significantly de-creased lattice thermal conductivity of~0.20 W m^(-1) K^(-1) and a high zT of~0.97 at 390 K are obtained in the Ag_(2)Se-based *** work demonstrates that such cell-membrane-mimic grain bound-ary engineering strategy may shed light on developing high-performance thermoelectric materials.

关键词： Thermoelectric Ag2 Se Grain boundary decoration High-dielectric-constant TiO_(2)

Low lattice thermal conductivity and enhanced thermoelectric performance of SnTe via chemical electroless plating of Ag

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Rare Metals 2022年第1期41卷 86-95页

作者： Bang-Zhou Tian xu-ping jiang Jie Chen Han Gao Ze-Gao Wang Jun Tang Da-Li Zhou Lei Yang Zhi-Gang Chen School of Materials Science&Engineering Sichuan UniversityChengdu 610064China Key Laboratory of Material Physics of Ministry of Education School of Physics and MicroelectronicsZhengzhou UniversityZhengzhou 450052China Key Laboratory of Radiation Physics and Technology Ministry of EducationInstitute of Nuclear Science and TechnologySichuan UniversityChengdu 610064China Centre for Future Materials University of Southern QueenslandSpringfield CentralQLD 4300Australia

Interface engineering has been regarded as an effective strategy to manipulate the thermoelectric performance of ***,we use a facile chemical electroless plating and a spark plasma sintering process to fabricate Ag-plated SnTe *** sintering,a small amount of plated Ag can be doped into SnTe to suppress the Sn vacancies and the others form Ag precipitates with a size distribution from nanoscale to microscale,which introduces Ag/SnTe interfaces to enhance the Seebeck coefficient via energy filtering ***,these structures result in strong scattering to reach a low lattice thermal conductivity of-0.62 W·m^(–1)·K^(–1).Consequently,a maximum figure of merit(zT)of-0.67 at 823 K is achieved in 2 wt%Ag-plated SnTe,which is-60%higher than that of pristine ***,the microhardness indentation test results show that the mean microhardness of 2 wt%Ag-plated SnTe is HV 64.26,which is much higher than that of pristine SnTe,indicating that Ag electroless plating can improve the mechanical properties of *** work has provided a facile and eco-friendly method to realize the interface engineering for manipulating the thermoelectric and mechanical properties of SnTe.

关键词： Thermoelectric SnTe Electroless plating Energy filtering effect Interface engineering

Multivariate model for predicting semen ：ryopreservation outcomes in a human sperm ban

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Asian Journal of Andrology 2017年第4期19卷 404-408页

作者： xu-ping jiang Wei-Min Zhou Shang-Qian Wang Wei Wang Jing-Yuan Tang Zhen xu Zhao-Xia Zhang Chao Qin Zeng-Jun Wang Wei Zhang Department of Urology Yixing People's Hospital Yixing 214200 China State Key Laboratory of Reproductive Medicine Department of Urology The First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China Human Sperm Bank Department of Urology The First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China

Semen cryopreservation is widely used in assisted reproductive technologies, but it reduces sperm quality dramatically. The aim of this study was to develop a model using basal semen quality to predict the outcome of postthaw semen parameters and improve the efficiency of cryopreservation in a human sperm bank. Basal semen parameters of 180 samples were evaluated in the first stage, and a multiple logistic regression analysis involving a backward elimination selection procedure was applied to select independent predictors. After a comprehensive analysis of all results, we developed a new model to assess the freezability of sperm. Progressive motility （PR）, straight-line velocity （VSL） and average path velocity （VAP） were included in our model. A greater area under the receiver operating characteristic curve was obtained in our model when compared with other indicators. In the second stage of our study, samples that satisfied the new model were selected to undergo freeze-thawing. Compared with the first stage, the rate of good freezability was increased significantly （94% vs 67%, P = 0.003）. By determining basal semen quality, we have developed a new model to improve the efficiency of cryopreservation in a human sperm bank.

关键词： cryopreservation freezability model sperm bank

Youjing granules ameliorate spermatogenesis in rats through regulating the prolifereation of spermatogonial stem cells

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Chinese Journal of Natural Medicines 2022年第8期20卷 580-588页

作者： jiang xu-ping SUN Yao-Xiang QIAO Bin ZHU Wen-jiao CHU Yu-Jiao SUN Miao-Miao ANG Ruije MA Tie-Liang ANG Zhi-An Department of Traditional Chinese Medicine the Affiliated Yixing Clinical School of Medical School of Yangzhou UniversityYixing 214200China Central Laboratory the Affiliated Yixing Hospital of Jiangsu UniversityYixing 214200China Department of Rehabilitation the People's Hospital of Daxinganling RegionDaxinganling 165000China School of Medicine School of Integrated Chinese and Western MedicineNanjing University of Chinese MedicineNanjing 210023China

Male infertility has evolved from a common reproductive system disease to a major social *** granule(YG)is a Chinese medicinal material used as a therapy method for tonifying the kidneys and removing dampness due to its pathogenic *** has been shown to regulate sperm quality in clinical trials,but the underlying mechanism is not fully *** present study was aimed to explore the protective effects and mechanism of action of YG on male reproductive system damage caused by methyl methane sulfonate(MMS).We first established an infertility model of rats through oral administration of MMS and then treated with *** determine the effect of YG,spermatogenesis,microvascular density,and secretory function of Leydig cells and Sertoli cells in rats were *** stem cells(SSCs)were co-cultured with mouse embryo fibroblast(MEF)cells as an in vitro cell model before exposure to serum containing ***,the proliferation and apoptosis of SSCs were *** indicated that YG increased the expression of self-renewal and proliferation-related molecules such as glial cell line derived neurotrophic factor(GDNF)and fibroblast growth factor-2(FGF2),and improved the quality of sperm and the proliferation of *** conclusion,YG may protect spermatogenetic function of rats through regulating the proliferation and self-renewal of SSCs.

关键词： Male infertility Youjing granules Spermatogenesis Spermatogonial stem cells

Study of Mechanisms and Therapeutics of Saponins from Anemarrhean Asphodeloides Bge on Alzheimei＇s disease

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中国药理通讯 2005年第4期22卷 17-18页

作者： Ouyang Shi xu jiang - ping Department Of Pharmacology Southern Medical University Guangzhou 510515

Phosphodiesterases:novel targets for treatment of alcoholism

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中国药理学与毒理学杂志 2017年第5期31卷 454-455页

作者： LIU Xin WEN Rui-ting GONG Mei-fang xu Ying Nicolas GRAHAME xu jiang-ping LIANG Jian-hui Marco CONTI 张汉霆 Institute of Pharmacology Taishan Medical University Departments of Behavioral Medicine & Psychiatry and Physiology & Pharmacology Blanchette Rockefeller Neurosciences InstituteWest Virginia University Health Sciences Center Department of Molecular and Cellular Pharmacology School of Pharmaceutical SciencesPeking University Department of Pharmacology School of Pharmaceutical SciencesSouthern Medical University Department of Pharmaceutical Sciences State University of New York at Buffalo Department of Psychology Indiana University-Purdue University Indianapolis Center for Reproductive Sciences Department of ObstetricsGynecology and Reproductive SciencesUniversity of California 泰山医学院药理学研究所泰山医学院药学院美国西弗吉尼亚大学医学院中国旅美科技协会西弗吉尼亚分会美国西弗吉尼亚华人协会

OBJECTIVE Alcoholism is one of the most damaging psychiatric disorders and causes serious social and health problems in the world. However,there are no ideal treatments for this disease in ***(PDEs) are a superfamily of enzymes consisting of 11 PDE families that hydrolyze cyclicAMP(cA MP) and/or cyclicGMP(cGMP). Among them,PDE4 is critical in the control of intracellular cAMP levels and has been shown to play an important role in the regulation of ethanol ***,the functional role of PDE4 in mediating alcoholism remains unclear. METHODS Ethanol drinking and preference were examined using the two-bottle choice and/or drinking-in-dark(DID) test in high alcohol preferring(HAP) animals,including C57,HAP,and PDE4-subtype knockout mice,and Fawn-Hooded(FH/Wjd) rats,treated with or without the PDE4 inhibitor rolipram or roflumilast. Ethanol withdrawal-induced anxiety-and depressive-like behaviors were examined using the elevated plusmaze,holeboard,forced-swim,and tail-suspension tests in C57 mice or FH rats in the presence of PDE4 inhibition. Levels of cAMP,CREB were determined in brain regions. RESULTS Treatment with rolipram or roflumilast decreased ethanol intake and preference in two-bottle choice and DID tests in C57 and HAP mice as well as FH rats. Mice deficient in PDE4 B,but not PDE4 D,displayed similar effects to general PDE4 inhibition. In addition,rolipram reversed ethanol withdrawal-induced anxietyand depressive-like behaviors 1 d and 14 d,respectively,following withdrawal from ethanol drinking in the two-bottle choice in C57 mice or FH rats. Locomotor activity was not changed in either mice or rats treated with the PDE4 inhibitors. Levels of cAMP,p CREB in the brain were increased by *** The results provide solid evidence for the important role of PDE4 in ethanol consumptionand ethanol withdrawal-induced symptoms. Inhibitors of PDE4,in particular the PDE4 B isoform,can be a novel class of treatment for alcohol

关键词： phosphodiesterase alcohol drinking alcoholism anxiety depression rodents

Inhibition of phosphodiesterase 4 by FCPR16 protects SH-SY5Y cells against MPP^+ -induced decline of mitochondrial membrane potential and oxidative stress

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中国药理学与毒理学杂志 2018年第9期32卷 699-700页

作者： ZHONG Jia-hong XIE Jin-feng XIAO Jiao LI Dan ZHOU Zhong-zhen WANG Hai-tao xu jiang-ping Department of Neuropharmacology and Drug Discovery Southern Medical University Guangzhou 510515 China Guangdong Provincial Key Laboratory of New Drug Screening School of Pharmaceutical Sciences Southern Medical University Guangzhou 510515 China

Parkinson disease(PD) is a chronic neurodegenerative disorder caused by progressive dopaminergic neuronal death in the substantia nigra pars compacta within the *** still is no cure,effective treatments for PD,available therapies are only capable of offering temporary and symptomatic relief to the *** are certain patents that claim phosphodiesterase(PDE) inhibitors as possible anti-PD drugs,PDE4 is a promising target for the treatment of PD and the underlying mechanism has not yet been well ***4 is an enzyme that specifically hydrolyzes intracellular cyclic adenosine monophosphate(cAMP)throughout the body,including the *** of the available PDE4 inhibitors exert unpleasant and serious side effects,such as emesis and nausea,which hinder its clinical ***,more efforts are needed before PDE4 inhibitors with high therapeutic indices are available for treatment of ***16 is a novel PDE4 inhibitor with little emetic potential,which exhibits excellent enzyme inhibition activity(IC50=90 nmol·L^(-1)).METHODS SH-SY5 Y cell was induced with 1-methyl-4-phenylpyridinium(MPP+)to mimic PD cell injury in vitro,and CCK-8 assay was used to investigate the viability effects of different concentration of FCPR16(3.1-50 μmol·L^(-1)) on MPP+-injured SH-SY5 Y *** of apoptosis was performed by flow *** level of ntracellular reactive oxygen species was detected with the fluorescent probe DCFH-DA,and the mitochondrial membrane potential of cells in different experimental groups was detected with the JC-1 fluorescent *** staining and Lysotracker Red staining were used to detect the intracellular antophagy *** expression of apoptosis related proteins,autophagy and other related signal molecules were demonstrated by Western *** cellular signaling pathway inhibitors were used to invesitigate the specific cellular mechanisms of FCPR16 protecting MPP+-induced cell *** FCPR16(12.5-

关键词： phosphodiesterase 4 FCPR16 oxidative stress mitochondrial membrane potential Parkinson disease

FCPR16 induces AMPK-dependent autophagy and provides neuroprotection in SH-SY5Y cells and neurons exposed to MPP^+-induced oxidative damage

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中国药理学与毒理学杂志 2019年第6期33卷 460-461页

作者： ZHONG Jia-hong XIE Jin-feng XIAO Jiao ZHOU Zhong-zhen WANG Hai-tao xu jiang-ping Department of Neuropharmacology and Drug Discovery School of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou510515China Guangdong Provincial Key Laboratory of New Drug Screening School of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou510515China

OBJECTIVE The cause of Parkinson disease (PD) is generally not clear, but it is considered to be related to excessive oxidative damage. Therefore, the identification of therapeutic targets and compounds with antioxidant damage is a reasonable strategy to slow down the progress of PD. FCPR16 is a novel phosphodiesterase4 inhibitor with little emetic potential. Our previous studies showed that FCPR16 was an effective compound for blocking 1-methyl-4-phenylpyridine(MPP+)-induced oxidative damage in SH-SY5Y cells and neurons. However, the detailed mechanisms underlying its protective effect have not been investigated. The level of oxidative stress in neurons is closely related to the balance of mitochondria mass, while autophagy strongly regulates mitochondrial activity in neurons. Our previous study indicated that inhibition of PDE4 or PDE4 knockdown enhanced the activation of autophagy in microglial cells. While whether PDE4 inhibition mediates autophagy in neurons is largely unknown. As described above, autophagy plays a pivotal role in maintaining redox and mitochondrial homeostasis. We were interested in exploring the impact of PDE4 inhibition on autophagy in neurons. METHODS SH-SY5Y cells and neurons was induced with MPP+to mimic PD cell injury in vitro, and MTT assay was used to investigate the viability effects of FCPR16 (50μmol·L-1) with or without different autophagy inhibitors on MPP+-injured SH-SY5Y cells. Detection of apoptosis was performed by PI staining fluorescence. Lysosomes are essential in autophagy, so LYT Red Stain was used to detect lysosomes in SY5Y cells and neurons. Cells were exposed to Cell ROX Deep Red Reagent to detect intracellular reactive oxygen species (ROS). Mitochondrial membrane potential (Δψm)measurement was executed by 5, 5′, 6, 6′-tetrachloro-1, 1′, 3, 3′-tetraethylbenzimidazolyl-carbocyanineiodide (JC-1).To better detect intracellular autophagy, we used the CYTO-ID Autophagy detection kit to detect the autophagic vacuoles and mon

关键词： phosphodiesterase 4 FCPR16 oxidative stress mitochondrial membrane potential Parkinson disease