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Relationship between survivin expression and recurrence,and prognosis in hepatocellular carcinoma

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World Journal of Gastroenterology 2007年第46期13卷 6264-6268页

作者： Chao-Ping Ye Cheng-Zhi Qiu zhong-xin Huang Qi-Chen Su Wei Zhuang Rui-Lan Wu xin-Feng Li Department of Hepatobiliary Surgery Second Affiliated Hospitalof Fujian Medical University Quanzhou 362000 Fujian ProvinceChina Departmentof Pathology Second Affiliated Hospital of Fujian MedicalUniversity Quanzhou 362000 Fujian Province China

AIM： To study the expression of the inhibitor of apoptosis protein survivin in hepatocellular carcinoma （HCC）, and its correlation with clinicopathological factors, cell proliferation, recurrence and prognosis after hepatectomy. METHODS： Immunohistochemical staining of survivin and Ki-67 was performed by the standard streptavidin- peroxidase technique on paraffin sections of 55 cases of HCC. RESULTS： The positive rate of survivin in HCC was 52.7% （29/55）. Significant correlation was found between survivin expression with portal vein thrombi and intrahepatic matastasistic nodes （P 〈 0.05）. The recurrent rate in survivin-positive HCC was significantly higher than that in survivin-negative HCC after hepatectomy, the 1- and 3-year survival rate in patients with survivin-positive tumors was significantly lower than that in patients with survivin-negative tumors （58.62 and 10.34% vs 76.92 and 30.77%, P 〈 0.05, log-rank test）. The proliferation index （Ki-67） in survivin-positive HCC （33.83% ± 18.90%） was significantly higher than that in survivin-negative HCC （19.60% ± 19.35%）（P 〈 0.05）. CONCLUSION： Survivin may play an important role in progression of HCC by promoting cell proliferation, and may be positively correlated with high risk of disease recurrence and poor prognosis in HCC. Its expression may serve as a prognostic factor for patients with HCC after hepatectomy.

关键词： Hepatocelluar carcinoma Survivin Proliferation index Prognosis Immunohistochemistry

Paramagnetism and improved upconversion luminescence properties of NaYF4：Yb,Er/NaGdF4 nanocomposites synthesized by a boiling water seed-mediated route

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Frontiers of Materials Science 2016年第1期10卷 38-44页

作者： Chao-Qing YANG Ao-Ju LI Wei GUO Peng-Hua TIAN Xiao-Long YU zhong-xin LIU Yang CAO zhong-Liang SUN Key Laboratory of Ministry of Education for Advanced Materials in Tropical Island Resources Hainan University Haikou 570228 China

In a route boiling water served as reaction medium, a stoichiometric amount of rare-earth compound and fluoride are put into this system to form a-NaYF4：Yb, Er nuclei. Then prepared sample is heated at elevated temperature to improve the fluorescence intensity, and next a NaGdF4 shell grows on the surface of NaYF4 nuclei. NaYF4：Yb,ErlNaGdF4 core-shell structured upconversion nanoparticles （CSUCNPs） have been successfully synthesized by above route. The use of boiling water decreases the cubic-to-hexagonal phase transition temperature of NaYF4：Yb,Er to 350℃ and increases its upconversion （UC） luminescence intensity. A heterogeneous NaGdF4 epitaxially growing on the surface of Ln^3＋-doped NaYF4 not only improves UC luminescence, but also creates a paramagnetic shell, which can be used as contrast agents in magnetic resonance imaging （MRI）o The solution of CSUCNPs shows bright green UC fluorescence under the excitation at 980 nm in a power density only about 50 ***-2. A broad spectrum with a dominant resonance at g of about 2 is observed by the electron paramagnetic resonance （EPR） spectrum of CSUCNPs. Above properties suggest that the obtained CSUCNPs could be potential candidates for dual-mode optical/magnetic bioapplications.

关键词： boiling water upconversion nanoparticle fluorescence imaging paramagnetism

Effects of Logging on the Genetic Diversity of Quercus tiaoloshanica Chun et Ko in a Tropical Montane Forest of Hainan Island, Southern China

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Journal of Integrative Plant Biology 2005年第10期47卷 1184-1192页

作者： Jian-Wei ZHENG Shu-Qing AN Lin CHEN xin LENG zhong-Sheng WANG Hua-Jun XIANG Laboratory of Forest Ecology and Global Changes School of Life Science Nanjing University Nanjing 210093 China

Quercus tiaoloshanica Chun et Ko, which has a narrow range of distribution, is one of the important endemic species of the tropical montane rain forest on Hainan Island, southern China. Long-term logging and habitat destruction have resulted in population decline and distribution retreat of Q. tiaoloshanica. To determine the impact of logging on the genetic diversity of Q. tiaoloshanica, the authors investigated the genetic structures using amplified fragment length polymorphism markers in four regenerated stands after logging and in one unlogged stand. Compared with the unlogged stand, the effective number of alleles per locus dropped by 1.0% in selective logging stands and by 2.0% in clear logging stands, corresponding to reductions of 3.8% and 5.2%, respectively, in mean Nei＇s gene diversity and 2.9% and 3.5%, respectively, in mean Shannon diversity index. No substantial genetic erosion was detected in any of the regenerated stands owing to the high tree density and high heterogeneity of the Q. tiaoloshanica stands investigated. Meanwhile, there was no natural regeneration of the species observed in a Dacrydium pierrei Hickel plantation 700 m away from the regenerated stands, suggesting the limited ability of seed dispersal of Q. tiaoloshanica. Clear logging should be undertaken cautiously because the total number of plant species dropped by 15.2% in the clear-logged stands compared with the unlogged stand. To conserve the genetic diversity of this species, as well as the plant biodiversity of tropical forests, the habitats of Q. tiaoloshanica should be protected against exploitation in terms of agricultural or other forms of land use, and some mature trees should be preserved as seed sources to maintain an adequate regeneration base for this species in the management of logging.

关键词： amplified fragment length polymorphism genetic erosion logging Quercus tiaoloshanica tropical forest.

Delamination process in cross-ply UHMWPE laminates under ballistic penetration

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Defence Technology（防务技术） 2021年第1期17卷 278-286页

作者： Gen-lin Mo Qian-wen Ma Yong-xi Jin Wen-min Yan zhong-xin Li Zhi-lin Wu Institute of Advanced Manufacturing Jiangsu UniversityZhenjiang212013China Key Laboratory of Transient Shock Beijing102202China Zhenjiang Hospital of Traditional Chinese Medicine 212013China School of Mechanical Engineering Nanjing University of Science and TechnologyNanjing210094China

Cross-ply unidirectional laminates made of ultra-high molecular weight polyethylene fibers are widely used as components of bullet-proof *** simulate the delamination process of the material under penetration,we constructed a numerical mechanical model,which was validated by tests using 7.6239 mm rifle bullets penetrating laminates of different *** results show that the delamination region is essentially *** simulated delamination region is in good agreement with the experimental *** is found that the delamination region increases with the compression modulus along the fiber *** delamination region increases when the shear strength between the fabric layers decreases;However,it is little influenced by the normal *** delamination region of the front face of the laminate is little influenced by the failure strain of the material and the initial velocity of the *** delamination region of the back face increases with the failure strain and decreases with the initial velocity.

关键词： proof process modulus

OPTIMIZED DESIGN OF NATURAL ECOLOGICAL WASTEWATER TREATMENT SYSTEM BASED ON WATER ENVIRONMENT MODEL OF DYNAMIC MESH TECHNIQUE

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Journal of Hydrodynamics 2010年第1期22卷 1-8页

作者： XU Zu-xin WEI zhong YIN Hai-long HUANG Li-hui State Key Laboratory of Pollution Control and Resource Reuse Tongji University Shanghai 200092 China College of Environmental Science and Engineering Shandong University Jinan 250100 China

A Natural Ecological Wastewater Treatment System （NEWTS） is usually built on natural terrain with necessary topography modification to improve water flowing route and pattern, and then the topography modified NEWTS should also have a reasonable water storage volume and hydraulic retention time so as to achieve the anticipated water purification effect. In this study, the dynamic mesh technique based on the finite element method and element storativity coefficients was presented to develop a two-dimensional hydrodynamic and water quality model, which was used to optimize the design of NEWTS under the dynamic land-water boundary due to various water storage volume. The models were employed in the optimized design of NEWTS from a large abandoned coal mine, which purifies the polluted water flowing into a large water storage lake, as part of the East Route South-to-North Water Transfer Project in China. Specifically, the natural topography modification scheme was presented, and further, a reasonable water storage volume and hydraulic residence time was obtained, based on the reasonable estimation of roughness coefficient and pollutant removal rate of the NEWTS with phragmites communis.

关键词： ecological wastewater treatment dynamic mesh hydrodynamic model water quality model abandoned coal mine

Highly coupled off-resonance lattice design in diffraction-limited light sources

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Nuclear Science and Techniques 2024年第9期35卷 92-102页

作者： Yi-Hao Gong Shun-Qiang Tian xin-zhong Liu Shou-Zhi Xuan Li-Yuan Tan Ling-Long Mao Shanghai Advanced Research Institute Chinese Academy of Sciences Shanghai 201204 ShanghaiChina Shanghai Institute of Applied Physics Chinese Academy of Sciences Shanghai 201800 ShanghaiChina University of Chinese Academy of Sciences 100049 BeijingChina

The round-beam operation presents many benefits for scientific experiments regarding synchrotron radiation and the weak-ening influences of intra-beam scattering in diffraction-limited synchrotron light sources.A round-beam generation method based on the global setting of skew quadrupoles and the application of a non-dominated sorting genetic algorithm was pro-posed in this *** schemes,including large-emittance coupling introduced via betatron coupling and vertical disper-sion,were explored in a candidate lattice for an upgrade-proposal of the Shanghai synchrotron radiation *** variations with lattice imperfections and their influence on the beam dynamics of beam optic distortions were *** results demonstrated that a precise coupling control ranging from 10 to 100%was achieved under low optical distortion,whereas full-coupling generation and its robustness were achieved by our proposed method by adjusting the skew quadrupole components located in the dispersion-free *** Touschek lifetime increased by a factor of 2–2.5.

关键词： Diffraction-limited storage ring(DLSR) Shanghai synchrotron radiation facility upgrade(SSRF-U) Round beam Off-resonance coupling

Sclerotinia sclerotiorum virulence is affected by mycelial age via reduction in oxalate biosynthesis

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Journal of Integrative Agriculture 2016年第5期15卷 1034-1045页

作者： WANG Ji-peng XU You-ping ZANG Xian-peng LI Shuang-sheng CAI xin-zhong Institute of Biotechnology College of Agriculture and Biotechnology Zhejiang University Center of Analysis and Measurement Zhejiang University

Sclerotinia sclerotiorum is one of the most devastating necrotrophic phytopathogens. Virulence of the hyphae of this fungus at different ages varies significantly. Molecular mechanisms underlying this functional distinction are largely unknown. In this study, we confirmed the effect of mycelial culture time/age on virulence in two host plants and elucidated its molecular and morphological basis. The virulence of the S. sclerotiorum mycelia in plants dramatically decreases along with the increase of the mycelial age. Three-day-old mycelia lost the virulence in plants. Comparative proteomics analyses revealed that metabolism pathways were comprehensively reprogrammed to suppress the oxalic acid（OA） accumulation in old mycelia. The oxaloacetate acetylhydrolase（OAH）, which catalyzes OA biosynthesis, was identified in the S. sclerotiorum genome. Both gene expression and protein accumulation of OAH in old mycelia were strongly repressed. Moreover, in planta OA accumulation was strikingly reduced in old mycelia-inoculated plants compared with young vegetative mycelia-inoculated plants. Furthermore, supply with 10 mmol L^（-1） OA enabled the old mycelia infect the host plants, demonstrating that loss of virulence of old mycelia is mainly caused by being unable to accumulate OA. Additionally, aerial mycelia started to develop from 0.5-day-old vegetative mycelia and dominated over 1-day-old mycelia grown on potato dextrose agar plates. They were much smaller in hypha diameter and grew significantly slower than young vegetative mycelia when subcultured, which did not maintain to progenies. Collectively, our results reveal that S. sclerotiorum aerial hyphae-dominant old mycelia fail to accumulate OA and thereby lose the virulence in host plants.

关键词： Sclerotinia sclerotiorum mycelial age oxalic acid virulence proteomics

Research advances of SAR remote sensing for agriculture applications: A review

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Journal of Integrative Agriculture 2019年第3期18卷 506-525页

作者： LIU Chang-an CHEN zhong-xin SHAO Yun CHEN Jin-song Tuya Hasi PAN Hai-zhu Key Laboratory of Agricultural Remote Sensing Ministry of Agriculture/Institute of Agricultural Resources and Regional PlanningChinese Academy of Agricultural SciencesBeijing 100081P.R.China Institute of Remote Sensing and Digital Earth Chinese Academy of SciencesBeijing 100101P.R.China Laboratory of Remote Sensing Information Science Shenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055P.R.China

Synthetic aperture radar(SAR) is an effective and important technique in monitoring crop and other agricultural targets because its quality does not depend on weather conditions. SAR is sensitive to the geometrical structures and dielectric properties of the targets and has a certain penetration ability to some agricultural targets. The capabilities of SAR for agriculture applications can be organized into three main categories: crop identification and crop planting area statistics, crop and cropland parameter extraction, and crop yield estimation. According to the above concepts, this paper systematically analyses the recent progresses, existing problems and future directions in SAR agricultural remote sensing. In recent years, with the remarkable progresses in SAR remote sensing systems, the available SAR data sources have been greatly enriched. The accuracies of the crop classification and parameter extraction by SAR data have been improved progressively. But the development of modern agriculture has put forwarded higher requirements for SAR remote sensing. For instance, the spatial resolution and revisiting cycle of the SAR sensors, the accuracy of crop classification, the whole phenological period monitoring of crop growth status, the soil moisture inversion under the condition of high vegetation coverage, the integrations of SAR remote sensing retrieval information with hydrological models and/or crop growth models, and so on, still need to be improved. In the future, the joint use of optical and SAR remote sensing data, the application of multi-band multi-dimensional SAR, the precise and high efficient modeling of electromagnetic scattering and parameter extraction of crop and farmland composite scene, the development of light and small SAR systems like those onboard unmanned aerial vehicles and their applications will be active research areas in agriculture remote sensing. This paper concludes that SAR remote sensing has great potential and will play a more

关键词： crop cropland yield soil roughness soil moisture LAI crop height scattering model quantitative remote sensing crop yield estimation SAR

Plasmid-based generation of neural cells from human fibroblasts using non-integrating episomal vectors

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Neural Regeneration Research 2019年第3期14卷 501-505页

作者： Shao-Bing Dai Ting Shen Ting-Ting Zheng Jia-Li Pu xin-zhong Chen Department of Anesthesiology Women's Hospital School of Medicine Zhejiang University Department of Neurology Second Affiliated Hospital College of Medicine Zhejiang University

Differentiation of human fibroblasts into functional neurons depends on the introduction of viral-mediated transcription factors, which present risks of viral gene integration and tumorigenicity. In recent years, although some studies have been successful in directly inducing neurons through sustained expression of small molecule compounds, they have only been shown to be effective on mouse-derived cells. Thus, herein we delivered vectors containing Epstein-Barr virus-derived oriP/Epstein-Barr nuclear antigen 1 encoding the neuronal transcription factor, Ascl1, the neuron-specific microRNA, miR124, and a small hairpin directed against p53, into human fibroblasts. Cells were incubated in a neuron-inducing culture medium. Immunofluorescence staining was used to detect Tuj-1, microtubule-associated protein 2, neuron-specific nucleoprotein NeuN and nerve cell adhesion molecules in the induced cells. The proportion of Tuj1-positive cells was up to 36.7% after induction for 11 days. From day 21, these induced neurons showed neuron-specific expression patterns of microtubule-associated protein 2, NeuN and neural cell adhesion molecule. Our approach is a simple, plasmid-based process that enables direct reprogramming of human fibroblasts into neurons, and provides alternative avenues for disease modeling and neurodegenerative medicine.

关键词： nerve regeneration induced neurons plasmid-based human fibroblasts nucleofection Ascl1 miR124 p53 reprogramming neural regeneration