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Fine mapping and characterization of stripe rust resistance gene YrAYH in near-isogenic lines derived from a cross involving wheat landrace Anyuehong

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The Crop Journal 2024年第3期12卷 826-835页

作者： li Long Jue li linyu Huang Huiling Jin Fangnian Guan Haipeng Zhang Sasa Zhao Hao li Zhien Pu Wei li Qiantao Jiang Yuming Wei Jian Ma Houyang Kang Shoufen Dai Pengfei Qi Qiang Xu Mei Deng Youliang Zheng Yunfeng Jiang Matthew James Moscou Guoyue Chen State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China Sichuan Agricultural UniversityChengdu 611130SichuanChina Triticeae Research Institute Sichuan Agricultural UniversityChengdu 611130SichuanChina College of Resources Sichuan Agricultural UniversityChengdu 611130SichuanChina The Sainsbury Laboratory Norwich Research ParkNorwich NR47UHEnglandUK United States Department of Agriculture-Agricultural Research Service Cereal Disease LaboratorySt.PaulMN 55108USA

Stripe rust,caused by Puccinia striiformis ***(Pst),is a devastating disease in wheat *** and characterizing new resistance genes/QTL is crucial for wheat breeding *** this study,we fine-mapped and characterized a stripe rust resistance gene,YRAYH,on chromosome arm 5BL in the Chinese wheat landrace Anyuehong(AYH).Evaluations of stripe rust response to prevalent Chinese Pst races in near-isogenic lines derived from a cross of Anyuehong and Taichung 29 showed that YrAYH conferred a high level of resistance at all growth *** mapping using a large segregating population of 9748 plants,narrowed the YRAYH locus to a 3.7 Mb interval on chromosome arm 5BL that included 61 annotated *** analysis of two NIL pairs identified 64 upregulated differentially expressed genes(DEGs)in the resistant NILs(NILs-R).Annotations indicated that many of these genes have roles in plant disease resistance *** a combined approach of fine-mapping and transcriptome sequencing,we identified a serine/threonine-protein kinase SRPK as a candidate gene underlying YrAYH.A unique 25 bp insertion was identified in the NILs-R compared to the NILs-S and previously published wheat *** InDel marker was developed and co-segregated with *** trait evaluation of the NILs suggested that YrAYH not only reduces the impact of stripe rust but was also associated with a gene that increases plant height and spike length.

关键词： Candidate gene analysis Crop protection Puccinia striiformis Transcriptome analyses

Study on the properties of solar cell under bias condition by using impedance spectroscopy

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Optoelectronics Letters 2024年第9期20卷 518-522页

作者： XIAO Wenbo li Ao XIAo Bangzhi Key Laboratory of Nondestructive Test Ministry of EducationNanchang Hangkong UniversityNanchang 330063China Science and Technology College Nanchang Hangkong UniversityGongqingcheng 332020China School of Engineering and Construction Nanchang UniversityNanchang 330031China

This paper investigates the properties of silicon cells(SI)and perovskite solar cells(PSC)under bias condition by using impedance *** parallel resistances R_(p) of SI and PSC are found to decrease with increasing bias,but the capacitance C_(p) shows the opposite *** Rp with C_(p),bias has a greater impact on the C_(p) of both cells.

关键词： spectroscopy impedance condition

Reoxidation of liquid steel and evolution of inclusions during protective atmosphere electroslag remelting of Ce-containing heat-resistant stainless steel

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Journal of Iron and Steel Research International 2024年第8期31卷 1923-1935页

作者： Yu Zhao Cheng-bin Shi Shijun Wang Peng Ren Jing li State Key Laboratory of Advanced Metallurgy University of Science and Technology Beijing(USTB)Beijing 100083China

The evolution of Ce-containing inclusions and its correlation with the reoxidation of liquid steel during protective atmosphere electroslag remelting(ESR)of heat-resistant steel were *** reoxidation of liquid steel took place during the ESR,resulting in the oxygen pickup from 0.0014 to 0.0053 wt.%.The inclusions in the consumable electrode,liquid metal pool and remelted ingot are Ce_(2)O_(2)S and Ce_(2)O,inclusions *** inclusions in the remelted ingot are originated from three ways:(I)the original inclusions from the electrode;(Il)the newly formed Ce_(2)O_(2)S and Ce_(2)O_(3) inclusions in the liquid metal pool by reoxidation remaining still in remelted ingot;(Ili)the newly generated Ce2O2S and Ce_(2)O_(3) inclusions during cooling and solidification of liquid *** relative proportions of Ce_(2)O_(3) inclusions in liquid metal pool and remelted ingot are 41% and 76.5%,*** inclusions ranging from 2 to 5μm in the remelted ingot take up 55%,followed by the inclusions smaller than 2μm(43%of total inclusions).The number proportion of the Ce_(2)0,inclusions in the liquid metal pool which were removed by floating up into slag is 1.96×10-5%.

关键词： Non-metallic inclusion Electroslag remelting Rare earth cerium Reoxidation Inclusion evolution

Lactobacillus melliventris promotes hive productivity and immune functionality in Bombus terrestris performance in the greenhouse

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Insect Science 2024年第3期31卷 911-926页

作者： Qianhui Yu Yan liu Shanshan liu Shaogang li Yifan Zhai Qingchao Zhang li Zheng Hao Zheng Yifan Zhai Xiaofei Wang Insttute of Plant Protection Shandong Academy of Agricultural SciencesJinanChina Key Laboratory of natural Enemies Insects Ministry of Agriculture and Rural AffairsJinanChina College of Food Science and Nutritional Engineering China Agricultural UniversityBeijingChina BGI-Qingdao BGI-ShenzhenQingdaoShandongChina

Bumblebees are important pollinators in agricultural ecosystems,but their abundance is declining *** is an urgent need to protect bumblebee health and their pollination *** possess specialized gut microbiota with potential to be used as probiotics to help defend at-risk bumblebee ***,evidence for probiotic benefits on bumblebees is ***,we evaluated how supplementation with Lactobacillus melliventris isolated from bumblebee gut affected the colony development of Bombus *** native strain colonized robustly and persisted long-term in bumblebees,leading to a significantly higher quality of ***,the tyrosine pathway was upregulated in the brain and fat body,while the Wnt and mTOR pathways of the gut were ***,the field experiment in the greenhouse revealed the supplementation of *** led to a 2.5-fold increase in the bumblebee survival rate and a more than 10%increase in the number of flowers visited,indicating a better health condition and pollination ability in field *** study represents a first screening for the potential use of the native gut member,***,as probiotic strains in hive supplement for bumblebee breeding,which may be a practical approach to improve immunity and hive health.

关键词： Bombus terrestris gut microbiota immunity Lactobacillus melliventris transcriptome

Clinical characteristics and impactof comorbidities on the prognosis of senileepilepsy in Southwest China:a retrospectivecohort study

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Acta Epileptologica 2024年第2期7卷 100-109页

作者： Zhen Cao Yinping li Shengyi liu Zihua He Jinmei li Department of Neurology West China HospitalSichuan UniversityChengdu 610041China Department of Geriatric The Second People’s Hospital of YibinYibin 644000China Department of Neurology Chengdu Shangjin Nanfu HospitalChengdu 610000China

Background Senile epilepsy and its comorbidities pose a tremendous burden on patients and the *** was aimed to investigate the clinical characteristics and comorbidities of senile epilepsy,as well as the impactof comorbidities on the prognosis of senile *** Information of patients with senile epilepsy was retrospectively collected from three tertiary hospitalsin Southwest China between December 2014 and December 2022.A total of 154 patients met the inclusion criteriaand were divided into two groups based on the presence or absence of *** prevalence,type,charac-teristics,and impacts of the comorbidities were *** characteristics of patients with and without comor-bidities were also *** Eighty-one percent of patients with senile epilepsy had at least one comorbidity,and 36%had three or *** different types of comorbidities were *** most common comorbidities were neu-rological(61%),followed by cardiovascular(45%)and psychiatric(26%)*** than one-third of patientshad bidirectional comorbidities,whereas more than half of the patients had additional causal *** types of comorbidities,neurological and psychiatric comorbidities were found to be associated with an increasedrisk of recurrent *** to patients without bidirectional comorbidities,those with at least one bidirec-tional comorbidity had a lower rate of achieving seizure *** higher the number of bidirectional comor-bidities,the lower the seizure-free *** analysis revealed that patients with neurological comorbidities hada higher risk of *** This study revealed a high comorbidity rate and a low seizure-freedom rate among patients with *** particular,neuropsychiatric comorbidities can increase the risk of seizures and affect the survival rateof patients with senile ***,preventing and managing these comorbidities may

关键词： Senile epilepsy Elderly Epilepsy Comorbidity Prognosis

Fine-tuning redox ability of arylene-bridged bis(benzimidazolium)for electrochromism and visible-light photocatalysis

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Chinese Chemical Letters 2024年第9期35卷 140-144页

作者： Jing Wang Zenghui li Xiaoyang liu Bochao Su Honghong Gong Chao Feng Guoping li Gang He Bin Rao School of Chemistry Engineering Research Center of Energy Storage Materials and DevicesMinistry of EducationXi’an Key Laboratory of Sustainable Energy Materials ChemistryXi’an Jiaotong UniversityXi’an 710054China Frontier Institute of Science and Technology Xi’an Jiaotong UniversityXi’an 710054China Instrumental Analysis Center Xi’an Jiaotong UniversityXi’an 710049China

In this study,a series of arylene-bridged bis(benzimidazolium)triflates 1^(–)6^(^(2+))·2[OTf^(–)]were synthesized by grafting differentπ-linkers with benzimidazolium *** them,compound 1^(2+)·2[OTf^(–)]with anthracene as the linker exhibited remarkable electron transfer capabilities across four distinct redox *** inclusion of an anthracene unit as theπ-linker contributes to its exceptional redox and optoelectronic ***,1^(2+)·2[OTf^(–)]was successfully utilized as both an electrochromic molecule in an ECD under applied voltage for the first time,and a highly efficient photocatalyst for the formation of carbon–phosphorus bonds via visible-light-induced cross-dehydrogenative coupling reactions.

关键词： N-Heterocyclic carbenes(NHCs) Ionic radicals Electrochromism Photocatalysis

nasal vaccination of triple-RBD scaffold protein with flagellin elicits long-term protection against SARS-CoV-2 variants including JN.1

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Signal Transduction and Targeted Therapy 2024年第5期9卷 2312-2323页

作者： Xian li Mengxin Xu Jingyi Yang li Zhou lin liu Min li Shasha Wang Mei-Qin liu Zhixiang Huang Zhen Zhang Shuning liu Yunqi Hu Haofeng lin Bowen liu Ying Sun Qingguo Wu Zheng-li Shi Ke Lan Yu Chen Huimin Yan Yao-Qing Chen Wuhan Institute of Virology Chinese Academy of SciencesWuhanChina Vaccine and Immunology Research Center Translational Medical Research InstituteShanghai Public Health Clinical CenterFudan UniversityShanghaiChina University of Chinese Academy of Sciences BeijingChina School of Public Health(Shenzhen) Shenzhen Campus of Sun Yat-sen UniversityShenzhenGuangdongChina State Key Laboratory of Virology Modern Virology Research CenterCollege of Life SciencesWuhan UniversityWuhanChina Aerosol Bio-Tech(Suzhou)Co. LTDSuzhouJiangsuChina national Medical Products Administration Key Laboratory for Quality Monitoring and Evaluation of Vaccines and Biological Products Sun Yat-sen UniversityGuanzhouChina

Developing a mucosal vaccine against SARS-CoV-2 is critical for combatting the ***,we investigated long-term immune responses and protection against SARS-CoV-2 for the intranasal vaccination of a triple receptor-binding domain(RBD)scaffold protein(3R-NC)adjuvanted with a flagellin protein(KFD)(3R-NC+KFDi.n).In mice,the vaccination elicited RBD-specific broad-neutralizing antibody responses in both serum and mucosal sites sustained at high level over a *** long-lasting humoral immunity was correlated with the presence of long-lived RBD-specific IgG-and IgA-producing plasma cells,alongside the Th17 and Tfh17-biased T-cell responses driven by the KFD *** upon these preclinical findings,an open labeled clinical trial was conducted in individuals who had been primed with the inactivated SARS-CoV-2(IAV)*** a favorable safety profile,the 3R-NC+KFDi.n boost elicited enduring broad-neutralizing IgG in plasma and IgA in salivary *** meet the challenge of frequently emerged variants,we further designed an updated triple-RBD scaffold protein with mutated RBD combinations,which can induce adaptable antibody responses to neutralize the newly emerging variants,including *** findings highlight the potential of the KFD-adjuvanted triple-RBD scaffold protein is a promising prototype for the development of a mucosal vaccine against SARS-CoV-2 infection.

关键词： vaccination vaccine sustained

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土壤真菌群落结构对辣椒长期连作的响应特征

环境科学 2024年第1期45卷 543-554页

作者：陈芬余高王谢丰李廷亮孙约兵铜仁学院贵州省梵净山地区生物多样性保护与利用重点实验室铜仁554300 山西农业大学资源环境学院晋中030801 农业农村部产地环境污染防控重点实验室天津市农业环境与农产品安全重点实验室天津300191

通过研究辣椒长期连作对土壤真菌群落结构特征的影响,揭示连作障碍机制,以期为辣椒产地生态安全和产业可持续发展提供理论依据.以铜仁市某蔬菜大棚种植基地辣椒连作土壤为研究对象,采用Illumina MiSeq高通量测序方法分析了农田土壤真菌... 详细信息

通过研究辣椒长期连作对土壤真菌群落结构特征的影响,揭示连作障碍机制,以期为辣椒产地生态安全和产业可持续发展提供理论依据.以铜仁市某蔬菜大棚种植基地辣椒连作土壤为研究对象,采用Illumina MiSeq高通量测序方法分析了农田土壤真菌多样性和群落结构,探讨了土壤理化性质和真菌群落特征对长期辣椒连作的响应,采用典范对应分析(CCA)和相关性网络分析了土壤真菌群落结构与环境因子的互作关系.结果表明,土壤pH和有机质(OM)含量随着连作年限的延长而降低,全磷(TP)和有效磷(AP)含量随着连作年限的增加而增加,水解氮(AN)和速效钾(AK)含量呈先降低后上升的趋势,全氮(TN)和全钾(TK)含量变化则不明显.长期连作先降低后提高Chao1指数和Observed species指数,降低了Shannon指数和Simpson指数.在门水平上被孢霉门(Mortierellomycota)的相对丰度随着连作年限的延长而降低,子囊菌门(Ascomycota)呈先增加后降低的趋势,担子菌门(Basidiomycota)则呈先降低后增加的趋势.在属水平上植物病原菌镰刀菌属(Fusarium)的相对丰度随着连作年限的延长而增加,有益菌被孢霉属(Mortierella)和青霉属(Penicillium)则呈逐渐降低趋势.此外,长期连作还使土壤真菌共生网络变得简单.CCA结果表明,pH、OM、TN、AN、AP和AK是连作土壤真菌群落结构组成的主要环境因子,相关性网络分析结果显示,pH、OM、TN、TP、TK、AN、AP和AK是Fusarium、瓶毛壳属(Lophotrichus)、Penicillium、Mortierella、毛葡孢属(Botryotrichum)、圆孢霉属(Staphylotrichum)、小不整球壳属(Plectosphaerella)和顶孢霉属(Acremonium)等真菌群落结构的主要环境因子.综上所述,连作改变了土壤的理化性质,影响了土壤真菌群落的多样性和群落结构,改变了微生物间的互作关系,破坏了土壤的微生态平衡,可能是导致辣椒连作障碍的主要原因.

关键词：辣椒连作真菌群落结构环境因子典范对应分析(CCA) 相关性网络分析

Intervention Effect of Lower limb Rehabilitation Robot with Task-Oriented Training on Stroke Patients and Its Influence on KFAROM Score

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Journal of Biosciences and Medicines 2024年第9期12卷 62-72页

作者： Maiding He li Huang Dekun Tang Mei Qin Ping Zhan Xichan Wang Xia Gao Jianzhu Wang na Yin Hui Xu Yonghua Yang Kaihua Tang Department of Rehabilitation Medicine Qujing No. 1 Hospital Qujing China

Objective: To explore the effect of lower limb rehabilitation robot combined with task-oriented training on stroke patients and its influence on KFAROM score. Methods: 100 stroke patients with hemiplegia admitted to our hospital from January 2023 to December 2023 were randomly divided into two groups, the control group (50 cases) was given task-oriented training assisted by nurses, and the observation group (50 cases) was given lower limb rehabilitation robot with task-oriented training. Lower limb balance, lower limb muscle strength, motor function, ankle function, knee flexion range of motion and walking ability were observed. Results: After treatment, the scores of BBS, quadriceps femoris and hamstrings in the observation group were significantly higher than those in the control group (P Conclusion: In the clinical treatment of stroke patients, the combination of task-oriented training and lower limb rehabilitation robot can effectively improve the lower limb muscle strength, facilitate the recovery of balance function, and have a significant effect on the recovery of motor function, which can improve the walking ability of stroke patients and the range of motion of knee flexion, and achieve more ideal therapeutic effectiveness.

关键词： Lower limb Rehabilitation Robot Task-Oriented Training Stroke KFAROM