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MDC-Net:a multi-directional constrained and prior assisted neural network for wood and leaf separation from terrestrial laser scanning

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International Journal of Digital Earth 2023年第1期16卷 1224-1245页

作者： wenxia Dai Yiheng Jiang Wen Zeng Ruibo Chen Yongyang Xu Ningning Zhu Wen xiao Zhen Dong Qingfeng Guan School of Geography and Information Engineering China University of GeosciencesWuhanPeople’s Republic of China Guangxi Zhuang Autonomous Region Institute of Natural Resources Remote Sensing NanningPeople’s Republic of China School of Computer Science China University of GeosciencesWuhanPeople’s Republic of China State Key Laboratory of Information Engineering in Surveying Mapping and Remote SensingWuhan UniversityWuhanPeople’s Republic of China National Engineering Research Center of Geographic Information System China University of GeosciencesWuhanPeople’s Republic of China

Wood-leaf separation from terrestrial laser scanning(TLS)is a crucial prerequisite for quantifying many biophysical properties and understanding ecological *** this study,we propose a novel multi-directional collaborative convolutional neural network(MDC-Net)that takes the original 3D coordinates and useful features from prior knowledge(prior features)as input,and outputs the semantic labels of TLS point *** MDC-Net contains two key units:(1)a multi-directional neighborhood construction(MDNC)unit to obtain more representative neighbors and enable directionally aware feature encoding in the subsequent local feature extraction,to mitigate occlusion effects;(2)a collaborative feature encoding(CFE)unit is introduced to incorporate useful features from prior knowledge into the network through a collaborative cross coding to enhance the discrimination for thin structures(*** branches and leaf).The MDC-Net is evaluated onfive plots from forests in Guangxi,China,with different branch architectures and leaf *** results showed that the MDC-Net achieved an OA of 0.973 and a mIoU of 0.821 and outperformed other related *** believe the MDC-Net would facilitate the usage of TLS in ecology studies for quantifying tree size and morphology and thus promote the development of relevant ecological applications.

关键词： Terrestrial laser scanning wood and leaf separation deep learning prior features

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Role of the anterior insular cortex microcircuits in observation fear of mice

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中国药理学与毒理学杂志 2023年第7期37卷 551-552页

作者： LI Fengan FU Yuan xiao Zhiyong SHI Tianyao ZHOU wenxia State Key Laboratory of Toxicology and Medical Countermeasures Beijing Institute of Pharmacology and Toxicology Nanjing University of Chinese Medicine

OBJECTIVE Fear can be learned indirectly, but excessive transmission of fear is essential for the development of mental illness. Previous research has indicated that the anterior insular cortex(AIC) may play a crucial role in the process of fear transmission,and abnormal AIC activity is a possible mechanism underlying various affective disorders. Inhibitory neurons are crucial for maintaining local microcircuit *** the support of novel specific neuroregulatory techniques, it is now possible to monitor and regulate different types of neurons in real-time. Therefore, investigating distinct subtypes of inhibitory neurons in the AIC that are involved in fear contagion may provide valuable insights into potential mechanisms underlying mental *** We established a modified observational fear(OF) model. A demonstrator(DM) mouse was placed in an acrylic cup at the center of the apparatus,and two observer(OB) mice were allowed to explore the DM mouse simultaneously from separate areas on either side. During the OF training, electric foot shocks were administered to the DM mouse and freezing, the side and corner time, and social interaction behavior were scored. Next, we characterized the activity patterns of distinct neuronal subtypes in the AIC using GCaMPbased calcium recording. Finally, we employed a Credependent optogenetic approach to selectively modulate excitatory or inhibitory neurons in the AIC, and investigated empathic fear behavior across different Cre transgenic mouse lines(CK2-Cre, PV-Cre, SOM-Cre, VIP-Cre).RESULTS During the training phase, the OB mice exhibited significantly higher levels of fear compared to the control group(which did not observe a traumatic event),as evidenced by increased freezing time, decreased interaction time, and increased corner zone time. Calcium fiber recording results suggested that CK2 neurons are involved in risk prediction, while PV and VIP neurons exert inhibitory control on this behavior. Optogenetic

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Targeting NRP1 axis as a strategy for treating energy metabolism impairment induced by SARS-CoV-2 spike

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Science China(Life Sciences) 2024年第10期67卷 2277-2279页

作者： Lihui Zhang Wanting Hu Jingxuan Li Yuehan Li Feng Liu Wenyi xiao Ning Jiang Zhiyong xiao Lu Han wenxia Zhou Beijing Institute of Pharmacology&Toxicology Beijing 100850China State Key Laboratory of Toxicology and Medical Countermeasures Beijing Institute of Pharmacology&ToxicologyBeijing 100850China School of Chinese Materia Medica Tianjin University of Traditional Chinese MedicineTianjin 301617China

Dear Editor,Free spike protein of SARS-CoV-2 alone can cause abnormal energy metabolism(Table S1 in Supporting Information),and limited research suggested a potential correlation between spike-induced mitochondrial da... 详细信息

关键词： metabolism ACE2

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Improvement of in situ LA-ICP-MS U-Pb dating method for carbonate minerals and its application in petroleum geology

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Science China Earth Sciences 2023年第12期66卷 2914-2929页

作者： Xuesong Lu Lili GUI Weiyan CHEN Shaobo LIU Shitou WU Junjia FAN Qiang LIU Jing SUN Liangliang ZHANG Yang xiao wenxia YANG Rizhou CAO Research Institute of Petroleum Exploration and Development Beijing 100083China Key Laboratory of Basin Structure and Hydrocarbon Accumulation CNPCBeijing 100083China Institute of Geology and Geophysics Chinese Academy of SciencesBeijing 100029China School of Geosciences China University of Petroleum(Beijing)Beijing 102249China Institute of Earth Sciences China University of Geosciences(Beijing)Beijing 100083China Sichuan Chuangyuan Weipu Analytical Technology Co. Ltd.Chengdu 610059China School of Geosciences Changjiang UniversityWuhan 430100China Research Institute of Exploration and Development Daqing Oilfield CoLtd.Daqing 163712China

In situ carbonate U-Pb dating is gaining popularity,and it has great potential for application in petroleum ***,the low U content(<10μg/g)and high common Pb content of carbonate minerals,along with the uneven distribution of U and Pb and the lack of matrix-matched reference material make carbonate U-Pb dating inaccurate and less successful,which limits the widespread application in *** study evaluated the limitations of in situ carbonate U-Pb dating and proposed a method to rationally determine the laser ablation parameters of samples by improving the experimental approach based on the laser ablation sector field inductively coupled plasma mass spectrometry(LA-SF-ICP-MS).By setting a different spot size and laser frequency for the reference material and unknown samples in the same session based on the U content of the sample,the ablation craters of the reference material and unknown samples were given the same depth/width ratio,avoiding systematic offset caused by differences in down-hole element fractionation and reducing the consume of reference *** on the heterogeneous distribution of U and Pb contents in carbonate minerals,the method of grid screening and setting ablation spots during screening were used to quickly select domains with high U and low common Pb,which improves the efficiency of setting laser spots and the success rate of dating,as well as reduces the experimental time and economic *** accuracy and success rate of carbonate U-Pb dating were effectively improved by improving the experimental method,and the technique was applied to two carbonate samples with low U and high common Pb contents that were difficult to date by traditional *** two samples are the saddle dolomite in the central Sichuan Basin and the calcite cement in the sandstone reservoir of the Cretaceous Qingshuihe Formation in the South Junggar *** robust ages have been obtained,which constrains the timing of the diagenetic and

关键词： Carbonate U-Pb dating LA-SF-ICP-MS Ablation craters Fractionation effects Systematic offset Critical time limitation

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Relationships among Deadwood Symptom Grades,Salicylic Acid Content and Diameter at Breast Height of Malus sieversii in China

Relationships among Deadwood Symptom Grades,Salicylic Acid C...

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中国植物病理学会2017年学术年会

作者： Yu Shaoshuai Zhao wenxia Yao Yanxia Huai wenxia xiao Wenfa Key Laboratory of Forest Protection of State Forestry Administration Research Institute of Forest EcologyEnvironment and ProtectionChinese Academy of Forestry

Malus sieversii distributed in Xinjiang,China is of serious exsciccation and its living condition is severely *** among deadwood symptom grades,salicylic acid(SA)content and diameter at breast height(DBH) of *** plants collected in the same sampling site and habitat conditions was analyzed to indicate phenotypic features of *** that maybe related to the symptoms of *** to the deadwood symptom,*** under the same sampling area and habitat was divided into 6 *** difference of salicylic acid composition and content in different symptom grades of *** was investigated by ultrasonic extraction,HPLC and statistics *** SA content in the different individuals was in the range of 3.61 0.25 mg/100 g,with significant difference(P 0 grade > Ⅳ grade > Ⅲ grade > Ⅰ grade(P 0 grade > Ⅱ grade > Ⅳ grade > Ⅲ grade > Ⅰ grade,basically same as the trend of the change of SA *** was a negative correlation between SA content and DBH of ***(P>0.05),which was similar to that of the samples with symptom of deadwood rate O.05).While there was positive correlation between SA content and DBH of the samples with symptom of deadwood rate > 40%(P > 0.05).There was a certain correlation among deadwood symptom grades,SA content and DBH of *** results achieved in the study would provide some reference clues for ecological conservation and restoration of wild fruit forest in Tianshan Mountains and screening and cultivation of high-quality varieties of cultivated apple.

关键词： Wild apple Deadwood rate Correlation analysis Stress resistance

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Development and application of specific SSR primers for Malus sieversii and cultivated apple

Development and application of specific SSR primers for Malu...

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中国植物病理学会第十一届全国会员代表大会暨2018年学术年会

TheSSR primers with specific,stable and polymorphic genes were designed and screened with the encoding gene sequences of Xinjiang wild apple(Malus sieversii) or cultivated apple resistance related proteins according to the complete genome sequencing of the completed genome of the cultivated *** newly developedSSR primers were screened and detected by PCR amplification and gene *** specific primers could be used to amplify specific,stable and polymorphicSSR bands in *** and cultivated apple,and 8 specific primers ofSSR could only amplifySSR stripe specifically in cultivated apples.290 samples of *** and cultivated apple were amplified and total 69 alleles were obtained using the 15 pairs of specificSSR *** is a close genetic relationship between *** and cultivated *** genetic diversity of *** is rich with a genetic differentiation coefficient of 0.248 6 and a gene flow of 0.755 *** *** from Xinyuan and Gongliu,is clustered in an evolutionary *** cultivated apple and the red meat apple collected from Xinjiang are clustered in an evolutionary *** number of alleles amplified by differentSSR loci was distinct and the frequencies of different alleles in the same microsatellite loci in different *** or cultivated apple groups were significantly different.15 pairs of specificSSR primers revealed the genetic diversity and phylogenetic relationship of *** and cultivated apples and the possible effects of genetic variation on the biological functions of *** and cultivated apple,exploring the genetic variation associated with resistance to ***.

关键词： Malus sieversii Microsatellite markers Genetic variation Stress resistance

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小波域的纹理图像快速修复算法

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仪器仪表学报 2008年第7期29卷 1422-1425页

作者：肖志云张文霞内蒙古工业大学信息工程学院呼和浩特010051

针对传统的空间域纹理图像修复算法计算量大、修复时间长的缺点,本文提出了一种纹理图像的快速修复算法。该算法的基本思想是在小波域中利用小波系数的能量来确定待修复块的填充顺序,并结合纹理合成的方法填充待修复区。实验结果表明,... 详细信息

针对传统的空间域纹理图像修复算法计算量大、修复时间长的缺点,本文提出了一种纹理图像的快速修复算法。该算法的基本思想是在小波域中利用小波系数的能量来确定待修复块的填充顺序,并结合纹理合成的方法填充待修复区。实验结果表明,该算法不仅可以大大提高纹理图像的修复速度,而且在峰值信噪比和主观视觉效果上都优于传统的图像修复算法。

关键词：图像修复小波变换纹理合成

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以猪粪为发酵底物厌氧发酵产氢工艺的优化

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农业工程学报 2009年第4期25卷 237-242页

作者：王媛媛张衍林华中农业大学工程技术学院武汉430070

为了建立发酵工艺参数与氢气产量之间的数学模型,以期获得较优的工艺参数,从而提高氢气产量,该文在单因素试验的基础上,采用三因素三水平的二次回归正交旋转组合设计及响应曲面分析法,建立了厌氧发酵产氢工艺中氢气产量的二次多项式数... 详细信息

为了建立发酵工艺参数与氢气产量之间的数学模型,以期获得较优的工艺参数,从而提高氢气产量,该文在单因素试验的基础上,采用三因素三水平的二次回归正交旋转组合设计及响应曲面分析法,建立了厌氧发酵产氢工艺中氢气产量的二次多项式数学模型,并以氢气产量为响应值作响应面和等高线,考察了以新鲜猪粪为发酵底物发酵产氢时初始pH值、水力停留时间和发酵底物中猪粪浓度3个因素及其交互作用对氢气产量的影响。分析结果表明,猪粪厌氧发酵产氢的较优工艺条件为:初始pH5.98,水力停留时间4.123d,猪粪干物质浓度51.98g/L;在此工艺条件下,氢气产率为32.4mL/g。

关键词：厌氧发酵优化工艺生物制氢猪粪

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受激发射损耗荧光显微镜的模型设计及参量优化

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光学学报 2006年第5期26卷 720-725页

作者：陈文霞肖繁荣刘力王桂英中国科学院上海光学精密机械研究所强场激光物理国家重点实验室上海201800

受激发射损耗荧光显微镜利用荧光饱和和激发态荧光受激损耗的非线性关系,通过限制损耗区域,可突破远场光学显微术的衍射极限分辨力并实现三维成像。基于对粒子速率方程组的修正,建立了描述荧光团各能级粒子数概率时间特性的模型,并定义... 详细信息

受激发射损耗荧光显微镜利用荧光饱和和激发态荧光受激损耗的非线性关系,通过限制损耗区域,可突破远场光学显微术的衍射极限分辨力并实现三维成像。基于对粒子速率方程组的修正,建立了描述荧光团各能级粒子数概率时间特性的模型,并定义了时间平均损耗效率判据。采用高斯函数模拟两束入射激光脉冲通过对模型的数值计算,模拟了激发脉冲的SIED激光脉冲的光强、脉冲宽度以及两束光的延迟时间等参量与损耗效率之间的关系,并获得了各参量的最佳值,优化了损耗效率,为提高系统分辨力提供了有效的途径。

关键词：显微损耗效率粒子数概率光强脉冲宽度延迟时间

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A new mouse model to study the empathic fear and pain behavior

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中国药理学与毒理学杂志 2023年第7期37卷 551-551页

作者： SHI Tianyao FU Yuan LI Fengan xiao Zhiyong ZHOU wenxia State Key Laboratory of Toxicology and Medical Countermeasures Beijing Institute of Pharmacology and Toxicology Nanjing University of Chinese Medicine

OBJECTIVE Human beings possess the ability to indirectly acquire the emotions of others. This also known as emotional contagion or empathy, enables us to rapidly perceive the emotions of others. However,an excessive empathy may result in heightened fear and sensitivity to pain. Therefore, the establishment of appropriate animal models for analyzing neural mechanisms underlying empathy would contribute to pharmacological research on pain sensitivity caused by psychological susceptibility. METHODS We used the observed fear paradigm for assessing contagion of negative emotions in mice. To minimize the impact of emotional contagion differences caused by the subject change, we established a bilateral observation area and the two mice were trained to observe fear simultaneously. First, two observer(OB)mice were placed on either side of the observational ***, a demonstrator(DM) mouse was introduced into the cylindrical shock cage located at the center of the apparatus. The shock cage is made of transparent organic plastic with air holes and has provided ample space for free movement by the DM mouse. During the shock stage, DM mice were subjected to electric stimulation while the behaviors of OB mice on both sides was observed, including freezing, the side and corner time,social interaction behavior. Additionally, c-Fos staining was utilized to confirm distinct local brain *** In the habituation stage, OB mice on both sides showed more social preference for DM mouse, as evidenced by an increase in duration time in the designated interaction zone. During the shock phase, OB mice observed the DM mouse receiving electric shocks and displayed significantly higher levels of fear contagion;however, their fear behavior was not entirely *** mice exhibited a significant increase in freezing time, while others demonstrated a significant increase in corner and side exploration time. We utilized Z-normalization to evaluate changes in emotionality across

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