Development and application of specific SSR primers for Malus sieversii and cultivated apple
作者单位：Key Laboratory of Forest Protection of State Forestry AdministrationResearch Institute of Forest EcologyEnvironment and ProtectionChinese Academy of Forestry
会议名称：《中国植物病理学会第十一届全国会员代表大会暨2018年学术年会》
会议日期：2018年
学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学]
基 金：National Key R&D Plan(2016YFC0501503)
关 键 词：Malus sieversii Microsatellite markers Genetic variation Stress resistance
摘 要：TheSSR primers with specific,stable and polymorphic genes were designed and screened with the encoding gene sequences of Xinjiang wild apple(Malus sieversii) or cultivated apple resistance related proteins according to the complete genome sequencing of the completed genome of the cultivated *** newly developedSSR primers were screened and detected by PCR amplification and gene *** specific primers could be used to amplify specific,stable and polymorphicSSR bands in *** and cultivated apple,and 8 specific primers ofSSR could only amplifySSR stripe specifically in cultivated apples.290 samples of *** and cultivated apple were amplified and total 69 alleles were obtained using the 15 pairs of specificSSR *** is a close genetic relationship between *** and cultivated *** genetic diversity of *** is rich with a genetic differentiation coefficient of 0.248 6 and a gene flow of 0.755 *** *** from Xinyuan and Gongliu,is clustered in an evolutionary *** cultivated apple and the red meat apple collected from Xinjiang are clustered in an evolutionary *** number of alleles amplified by differentSSR loci was distinct and the frequencies of different alleles in the same microsatellite loci in different *** or cultivated apple groups were significantly different.15 pairs of specificSSR primers revealed the genetic diversity and phylogenetic relationship of *** and cultivated apples and the possible effects of genetic variation on the biological functions of *** and cultivated apple,exploring the genetic variation associated with resistance to ***.