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Establishment of a Multiplex Detection Method for Common Bacteria in Blood Based on Human Mannan-Binding Lectin Protein-Conjugated Magnetic Bead Enrichment Combined with Recombinase-Aided PCR Technology

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Biomedical and Environmental sciences 2024年第4期37卷 387-398页

作者： ZHAO Zi jin CHEN {1. Ping HUA Shao Wei LI Feng Yu ZHAO Meng XING Chen Hao WANG {2. TIAN Feng Yu {3. Rui Qing LYU {1. Na HAN Zhi Qiang WANG Yu Xin LI Hong Yi SHEN Xin Xin MA Xue Jun TIE Yan Qing Graduate School Hebei North UniversityZhangjiakou 075000HebeiChina Department of Clinical Laboratory Hebei General HospitalShijiazhuang 050051HebeiChina National Key Laboratory of Intelligent Tracking and Forecasting for Infectious Diseases NHC Key Laboratory of Medical Virology and Viral DiseasesNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China National institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China Department of Clinical Laboratory Pathology Diagnostic Center Shiyan General HospitalShiyan 442000HubeiChina Graduate School Hebei Medical University Shijiazhuang 050031HebeiChina

Objective Recombinase-aided polymerase chain reaction(RAP)is a sensitive,single-tube,two-stage nucleic acid amplification *** study aimed to develop an assay that can be used for the early diagnosis of three types of bacteremia caused by Staphylococcus aureus(SA),Pseudomonas aeruginosa(PA),and Acinetobacter baumannii(AB)in the bloodstream based on recombinant human mannanbinding lectin protein(M1.protein)-conjugated magnetic bead(M1.bead)enrichment of pathogens combined with *** Recombinant plasmids were used to evaluate the assay *** blood influenza bacteria were used for the specific *** and clinical plasma samples were enriched with M1.beads and then subjected to multiple recombinase-aided PCR(M-RAP)and quantitative PCR(qPCR)*** analysis was used to evaluate the consistency between the two *** The M-RAP method had sensitivity rates of 1.1.,and 1.copies/μL for the detection of SA,PA,and AB plasmids,respectively,without cross-reaction to other bacterial *** M-RAP assay obtained results for1. CFU/mL pathogens in the blood within 4 h,with higher sensitivity than qPCR.M-RAP and qPCR for SA,PA,and AB yielded Kappa values of 0.83.,0.81.,and 0.856,respectively(P1.bead enrichment has been developed and can be potentially used for the early detection of bacteremia.

关键词： Staphylococcus aureus Pseudomonas aeruginosa Acinetobacter baumannii Human Mannan-binding lectin protein Bloodstream infection Recombinase-aided PCR assay Multiple detection

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Rational redesign of inhibitors of furin/kexin processing proteases by electrostatic mutations

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Acta Pharmacologica Sinica 2004年第12期25卷 146-152页

作者： xiao-hui CAI, Qing {1., Da-fu DING2.Key Laboratory of Proteomics, {2. of Biochemistry and Cell Biology, Shanghai {2.s for Biological sciences, chinese academy of sciences, Graduate School of the chinese academy of sciences, Shanghai 2.003., China Key Laboratory of Proteomics Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Graduate School of the Chinese Academy of Sciences Shanghai 200031 China

AIM: To model the three-dimensional structure and investigatethe interaction mechanism ofthe proprotein convertase furin/kexin and their inhibitors (eglin c mutants). METHODS: The three-dimensional complex structures of furin/ kexin with its inhibitors, eglin c mutants, were generated by modeller program using the newly published X-ray crystallographical structures of mouse furin and yeast kexin as templates. The electrostatic interaction energy of each complex was calculated and the results were compared with the experimentally determined inhibition con- stants to find the correlation between them. RESULTS: High quality models of furin/kexin-eglin c mutants were obtained and used for calculation of the electrostatic interaction energies between the proteases and their inhibitors. The calculated electrostaticenergies of interaction showeda linear correlation to the experimental inhibition constants. CONCLUSION: The modeled structures give good explanations of the specificity of eglin c mutants to furin/ kexin. The electrostatic interactions play important roles in inhibitory activity of eglin c mutants to furin/kexin. The results presented here provided quantitative structural and functional information concerning the role of the charge- charge interactions in the binding of furin/kexin and their inhibitors.

关键词： furin kexin eglin c drug design electrostatic mutation

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Engineered AAV1.0}variants with enhanced transduction and confined spread

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Zoological Research 2024年第4期45卷 781-790页

作者： Neng-Song Luo Yu-Xiang Cai Zeng-Peng Han xiao-Kai Sui Wen-Jia Yuan Zi-Lian {1. Hao-Dong Guo {2. Wang Kun-{1. Lin Fu-Qiang Xu wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhanHubei 430074China Shenzhen Key Laboratory of Viral Vectors for Biomedicine Shenzhen-Hong Kong Institute of Brain ScienceShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong 518055China Key Laboratory of Magnetic Resonance in Biological Systems State Key Laboratory of Magnetic Resonance and Atomic and Molecular PhysicsNational Center for Magnetic Resonance in WuhanInnovation Academy for Precision Measurement Science and TechnologyChinese Academy of SciencesWuhanHubei 430071China Key Laboratory of Quality Control Technology for Virus-Based Therapeutics Guangdong Provincial Medical Products AdministrationNMPA Key Laboratory for Research and Evaluation of Viral Vector Technology in Cell and Gene Therapy Medicinal ProductsBrain Cognition and Brain Disease InstituteShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhenGuangdong 518055China University of chinese academy of sciences Beijing 100049China Zhongnan Hospital Wuhan UniversityWuhanHubei 430072China Brain Case Biotechnology Co. Ltd.ShenzhenGuangdong 518107China College of Life sciences Wuhan UniversityWuhanHubei 430072China Center for Excellence in Brain Science and Intelligence Technology Chinese Academy of SciencesShanghai 200031China

Precise targeting of specific regions within the central nervous system(CNS)is crucial for both scientific research and gene therapy in the context of brain ***-associated virus 13.AAV13.is known for its restricted diffusion range within the CNS,making it an ideal choice for precise labeling and administration within small brain ***,AAV13.mediates relatively low expression of target ***,we introduced specifically engineered modifications to the AAV13.capsid protein to enhance its transduction *** first constructed AAV13.YF by mutating tyrosine to phenylalanine on the surface of the AAV13.*** then inserted the 7m8 peptide,known to enhance cell transduction,into positions 587/588 and 585/586 of the AAV13.capsid,resulting in two distinct variants named AAV13.587-7m8 and AAV13.585-7m8,*** found that AAV13.YF exhibited superior in vitro infectivity in HEK2.3. cells compared to AAV13.while AAV13.587-7m8 and AAV13.585-7m8 showed enhanced CNS infection capabilities in C57BL/6 mice,with AAV13.587-7m8 infection retaining a limited spread *** modified AAV13.variants hold promising potential for applications in gene therapy and neuroscience research.

关键词： Adeno-associated virus 1. AAV1.0}YF AAV1.0}587-7m8 AAV1.0}585-7m8 transduction efficiency

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Folding encapsulation toward Lindqvist polyoxometalates within adaptive coordination cages for catalysis

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Science China Chemistry 2024年

作者： Ying-Mei Zhong jin-jin Xuan Xu-{2. Qin Han {3. Zi-Jun Xia Li-Peng Zhou {3.-Qing Guo Yu-Hang Hu Lu zhang Chun-Sen Li Li-Xuan Cai Qing-Fu Sun State Key Laboratory of Structural Chemistry Fujian Institute of Research on the Structure of Matter Chinese Academy of Sciences College of Chemistry and Materials Science Fujian Normal University University of chinese academy of sciences

In nature,enzymes at specific folded conformations exhibit high substrate complementarity,enabling them to efficiently and selectively catalyze biochemical *** by enzymes,coordination cages with conformational dynamics have been designed and are receiving increasing attention due to their versatile applications in allosteric guest binding and *** present here two enzyme-mimicking adaptive macrocycle-based coordination cages,Pd2.1.0}nd Pd2.2.,from macrocycle-based ligands（L1.nd L2. and the encapsulation and stabilization of Lindqvist polyoxometalates anions M6O1.2.（M=W or Mo） in neutral aqueous solution.X-ray crystallographic studies have verified the formation of 1.1.host-guest complexes of two Pd2.L2.cages and the W6O1.2.anion,via an induced-fit folding *** with the free host,Pd2.1.0}xhibited an induced-fit semi-folded conformation in the inclusion complex,while Pd2.2.adopted an induced-fit fully-folded ***,the Mo6O1.?Pd2.2.ost-guest complexes demonstrate high yield and selectivity in the oxidative decontamination reaction of the sulfur mustard simulant,2.chloroethyl ethyl *** work not only sheds light on the importance of conformational adaptability in enzyme-like systems but also paves the way for protecting labile POM clusters via host-guest encapsulation toward enhanced catalytic performance in aqueous solution.

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Genus Gentianella Moench:A Phytochemical and Ethnopharma-cological Review

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chinese Herbal Medicines 2010年第4期2卷 262-271页

作者： LI Min-hui1, LI Li2. 3. YANG Yu-mei1, zhang Na1, SONG xiao-ling1, xiao Pei-gen2. 3.1. Baotou Medical College, Baotou 014060, China 2. School of {3. Materia Medica, Beijing University of {3. Medicine, Beijing 100102. China 3. institute of Medicinal Plant Development, {3. {13. of Medical sciences, Beijing 100193. China Baotou Medical College Baotou 014060 China School of chinese Materia Medica Beijing University of Chinese Medicine Beijing 100102 China institute of Medicinal Plant Development Chinese Academy of Medical Sciences Beijing 100193 China

The plants in genus Gentianella Moench (Gentianaceae) which comprised approximately 2.0 species, are mainly distributed in temperate regions of the world. Many Gentianella plants are intensely bitter and employed in traditional medicine to stimulate appetite, treat disorders of the gallbladder, and treat fever like the other bitter gentians in various regions of the world. Some species exhibit other remarkable therapeutic effects in the treatments of obesity, diabetes, and heart diseases. Eleven iridoids, twenty-eight xanthones, three C-glucoflavonoids, and eight other compounds have been isolated from the genus. Most of these compounds are associated with antimicrobial, anti-inflammatory, anti-oxidant, hypoglycemic, and antitumor activities, which provide an empirical base for the traditional utilization of the plants in genus Gentinella Moench.

关键词： ethnopharmacology Gentianella Moench pharmacology phytochemistry traditional medicine

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A conserved structural motif shared by EIAV gp90 and HIV gp1.0}

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Science Bulletin 2005年第20期 139-140页

作者： LI Huiguang1, TONG xiao1,2. SHEN Tao1, SHEN Rongxian3. zhang xiaoyan1 & SHAO Yiming1 1. Department of Research on Virology and Immunology, National Cen- ter for AIDS/STD Control and Prevention, CDC, Beijing 100052. China 2. {3. institute of Virology, chinese academy of sciences, {3. 43.071, China 3. Department of Research on EIAV, Harbin Veterinary Research Insti- tute, Harbin 150001, China

Both HIV and EIAV belong to the retroviridae family and lentivirus genus. They share considerable simi- larity at gene structural and component level. Hotzel pro- posed that two variable regions and surrounding amino acids of EIAV gp90 might adopt the same topology as V1. V2.and bridging sheet of HIV gp1.0}. These regions map to V3. V4 and surrounding amino acids of EIAV gp90. Based on this proposal, we further analyzed the distribution profile of N-glycosylation site and linear epitope for EIAV gp90 and HIV gp1.0} at these regions. Our results demonstrated that EIAV gp90 and HIV gp1.0} are highly similar at these two aspects. Most likely, V3. V4 and surrounding amino acids of EIAV gp90 adopt the same topology structure as V1. V2.and bridging sheet of HIV gp1.0}.

关键词： EIAV HIV N-glycosylation epitope gp1.0} gp90.

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CAS-ESM2.0 Dataset for the Carbon Dioxide Removal Model Intercomparison Project(CDRMIP)

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Advances in Atmospheric sciences 2024年第5期41卷 989-1000页

作者： Jiangbo jin Duoying JI {1. DONG Kece FEI Run GUO Juanxiong HE Yi YU Zhaoyang CHAI He {2. Dongling {2. Kangjun CHEN Qingcun ZENG International Center for Climate and Environment sciences Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China State Key Laboratory of Satellite Ocean Environment Dynamics Second Institute of OceanographyMinistry of Natural ResourcesHangzhou 310012China State Key Laboratory of Earth Surface Processes and Resource Ecology College of Global Change and Earth System ScienceBeijing Normal UniversityBeijing 100875China State Key Laboratory of Numerical Modeling for Atmospheric sciences and Geophysical Fluid Dynamics Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China

Understanding the response of the Earth system to varying concentrations of carbon dioxide(CO_(2.)is critical for projecting possible future climate change and for providing insight into mitigation and adaptation strategies in the near *** this study,we generate a dataset by conducting an experiment involving carbon dioxide removal(CDR)—a potential way to suppress global warming—using the{1. Earth System Model version 2.0(CASESM2.0).A preliminary evaluation is *** model is integrated from 2.0–3.0 years as a 1.yr^(−1. CO_(2. concentration increase experiment,and then to~478 years as a carbon dioxide removal experiment until CO_(2. returns to its original ***,another 80 years is integrated in which CO_(2. is kept *** in the 2.m temperature,precipitation,sea surface temperature,ocean temperature,Atlantic meridional overturning circulation(AMOC),and sea surface height are all *** the ramp-up period,the global mean 2.m temperature and precipitation both increase while the AMOC *** of all the above variables change in the opposite direction in the ramp-down period,with a delayed peak relative to the CO_(2. *** CO_(2. returns to its original value,the global mean 2.m temperature is still~1.K higher than in the original state,and precipitation is~0.07 mm d^(–1. *** the end of the simulation,there is a~0.5°C increase in ocean temperature and a 1.Sv weakening of the *** model simulation produces similar results to those of comparable experiments previously reported in the literature.

关键词： CAS-ESM2.0 CDRMIP carbon dioxide removal AMOC temperature precipitation sea surface height

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Temperature variation and its driving forces over the Antarctic coastal regions in the past 2.0 years

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Journal of Geographical sciences 2002年第4期12卷 379-386页

作者： zhang Mingjun1. {1. REN Jiawen{1. CHENG Guodong{1. LI Zhongqin{1.xiao Cunde{1. QIN Dahe{1. KANG Jiancheng3. LI Jun4(1. College of Geography and Environment, Northwest Normal University, Lanzhou 73.070, China {1. Cold and Arid Regions Environmental and Eng College of Geography and Environment Northwest Normal University Lanzhou 730070 Cold and Arid Regions Environmental and Engineering Research institute CAS Lanzhou 730000 chinese {1. of Polar Research 上海 200129 Antarctic Cooperation Research Center and Australian Antarctic Division 澳大利亚 Hobart

By comparing the oxygen isotopic temperatures recorded by many shallow ice cores from the coastal regions of Antarctica, this paper presents the special characteristics of the temperature variations over the Antarctic coastal regions in the past 50 years, 1.0 years and 2.0 years. In the past 50 years, the isotopic temperatures recorded in the ice cores over different sites on the Antarctic coastal regions differ greatly. For instance, although increasing isotopic temperatures have been reported for many sites studied, many sites show decreasing trends, the regional regularity in temperature variations is still insignificant. In the past 1.0 years, the isotopic temperature trends in the coastal regions of Antarctica show an alternate-distributing pattern. In the past 2.0 years, all the ice cores from the coastal regions of Antarctica have recorded the so-called Little Ice Age (LIA). The above-mentioned spatial characteristics of the temperature variations over the Antarctic coastal regions are likely to reflect the impacts of the unique Southern Hemisphere atmospheric circulation, the Antarctic Circumpolar Wave (ACW) and the special terrain (such as the large drainage basins) on the coastal regions of Antarctica. Furthermore, the impacting intensity of the unique Southern Hemisphere atmospheric circulation, the Antarctic Circumpolar Wave and the special terrain differs in terms of the temporal scale of the temperature change.

关键词： Antarctica ice core temperature terrain Antarctic Circumpolar Wave (ACW) circumpolar circulation

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1.B-doped MCP detector developed for neutron resonance imaging at Back-n white neutron source

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Nuclear Science and Techniques 2024年第8期35卷 58-68页

作者： Qiang Li Li‑Jiao Wang jing‑Yu Tang Xiang‑Biao Qiu Zhen Chen Mao‑Yuan Zhao Chang‑Jun Ning Kai Pan Wei Xu Tao Li Su‑Peng Lu Han Yi Rui‑Rui Fan Chang‑Qing Feng Rong {1. {2.‑Yang Sun Qi An Hao‑Fan Bai Jiang‑Bo Bai {3. Bao Ping Cao Qi‑Ping Chen Yong‑Hao Chen Zeng‑Qi Cui An‑Chuan Fan Fan‑Zhen Feng Min‑Hao Gu Chang‑Cai Han Zi‑{3. Han Guo‑Zhu He Yong‑Cheng He Yang Hong Yi‑Wei Hu Han‑Xiong Huang Wei Jiang Zhi‑{3. Jiang Zheng‑Yao jin Ling Kang Bo Li Gong Li {2. Li Yang Li {3. Liu Rong Liu Shu‑Bin Liu Yi‑Na Liu Guang‑Yuan Luan {3. Ren Zhi‑Zhou Ren Xi‑Chao Ruan Zhao‑Hui Song Kang Sun Zhi‑Xin Tan Sheng‑Da Tang jin‑Cheng Wang Peng‑Cheng Wang Zhao‑Hui Wang Zhong‑Wei Wen {2.‑Guang Wu Xuan Wu Cong Xia Yong‑Ji Yu Guo‑Hui {1. Hang‑Chang {1. Lin‑Hao {1. Qi‑Wei {1. Xian‑Peng {1. Yu‑Liang {1. Yue {1. Zhi‑Yong {1. Zhi‑Hao Zhou Ke‑Jun Zhu Chong Zou institute of High Energy Physics Chinese Academy of Sciences(CAS)Beijing 100049China Spallation Neutron Source Science Center Dongguan 523803China State Key Laboratory of Particle Detection and Electronics Institute of High Energy Physics Chinese Academy of SciencesBeijing 100049China Dongguan Songshan Lake Future School Dongguan 523808China School of Nuclear Science and Technology University of Science and Technology of ChinaHefei 230027China State Key Laboratory of Particle Detection and Electronics University of Science and Technology of ChinaHefei 230026China North Night Vision Science and Technology(Nanjing)Research {1. Co.Ltd Nanjing 211106China Department of Modern Physics University of Science and Technology of ChinaHefei 230026China State Key Laboratory of Nuclear Physics and Technology School of PhysicsPeking UniversityBeijing 100871China institute of Modern Physics Fudan UniversityShanghai 200433China Key Laboratory of Nuclear Data China Institute of Atomic EnergyBeijing 102413China China academy of Engineering Physics Institute of Nuclear Physics and ChemistryMianyang 621900China USTC archaeometry lab University of Science and Technology of ChinaHefei 230026China Northwest institute of Nuclear Technology Xi’an 710024China University of chinese academy of sciences Beijing 100049China China Shipbuilding Trading Co. LtdBeijing 100044China

Neutron resonance imaging(NRI)has recently emerged as an appealing technique for neutron *** complexity surpasses that of conventional transmission imaging,as it requires a high demand for both a neutron source and ***,the progression of NRI technology has been sluggish since its inception in the 1.80s,particularly considering the limited studies analyzing the neutron energy range above *** white neutron source(Back-n)at the China Spallation Neutron Source(CSNS)provides favorable beam conditions for the development of the NRI technique over a wide neutron energy range from eV to ***-sensitive microchannel plates(MCP)have emerged as a cutting-edge tool in the field of neutron detection owing to their high temporal and spatial resolutions,high detection efficiency,and low *** this study,we report the development of a 1.B-doped MCP detector,along with its associated electronics,data processing system,and NRI experiments at the *** heavy elements such as gold,silver,tungsten,and indium can be easily identified in the transmission images by their characteristic resonance peaks in the 1.1.0 eV energy range;the more difficult medium-weight elements such as iron,copper,and aluminum with resonance peaks in the 1.1.0 keV energy range can also be *** particular,results in the neutron energy range of dozens of keV(Aluminum)are reported here for the first time.

关键词： Neutron resonance imaging 1.B doped MCP detector White neutron source Sample nuclide identification

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Investigation of the differences between the “COLD” and “HOT” nature of Coptis chinensis Franch and its processed materials based on animal’s temperature tropism

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Science China(Life sciences) 2009年第11期52卷 1073-1080页

作者： ZHOU CanPing1,2. WANG JiaBo1, zhang XueRu1,2. ZHAO YanLing1, XIA XinHua2. ZHAO HaiPing1, REN YongShen1 & xiao xiaoHe1 1 China Military {3. of chinese Meteria Medica, 3.2.Military Hospital of China, Beijing 10003., China 2.College of Pharmaceutical sciences, Hunan University of chinese Medicine, Changsha 4102.8, China China Military institute of {1. Meteria Medica 302 Military Hospital of China Beijing 100039 China College of Pharmaceutical sciences Hunan University of Chinese Medicine Changsha 410208 China

The description and differentiation of the so-called "Cold" and "Hot" natures, the primary "Drug Naure" of chinese medicine, is the focus of theoretical research. In this study, the divergency between the "Cold" and the "Hot" natures was investigated through examining the temperature tropism of mice affected by Coptis chinensis Franch and its processed materials by using a cold/hot plate differentiating technology. After exposure to C. chinensis Franch, the macroscopic behavioral index of the remaining rate (RR) on a warm pad (40℃ ) significantly increased (Pchinese medicine may possibly reflect in an ethological way for the changes of animal’s temperature tropism which might be internally regulated by the body’s energy metabolism.

关键词： "Cold" and "Hot" natures of chinese medicine evaluation temperature tropism ethology drug process Coptis chinensis Franch

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