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Dynamic changes of behaviors, dentate gyrus neurogenesis and hippocampal miR-124 expression in rats with depression induced by chronic unpredictable mild stress

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Neural Regeneration Research 2020年第6期15卷 1150-1159页

作者： Yun-Ling Huang ning-xi Zeng Jie Chen Jie Niu Wu-Long Luo Ping Liu Can Yan Li-Li Wu Research Center for Basic Integrative Medicine Guangzhou University of Chinese MedicineGuangzhouGuangdong ProvinceChina Department of Pharmacology PLA General HospitalBeijingChina

The depression-like behavior phenotype,neurogenesis in the dentate gyrus and miR-124 expression in the hippocampus are the focus of current research on the pathogenesis of depression and antidepressant *** present study aimed to clarify the dynamic changes of depression-like behavior,dentate gyrus neurogenesis and hippocampal miR-124 expression during depression induced by chronic stress to reveal pathological features at different stages of depression and to further provide insight into depression *** unpredictable mild stress depression models were established by exposing Sprague-Dawley rats to various mild stressors,including white noise,thermal swimming,stroboscopic illumination,soiled cages,pairing with three other stressed animals,cold swimming,tail pinch,restraint and water and food *** unpredictable mild stress model rats underwent dynamic observation from 1 to 8 weeks and were compared with a control group(normal feeding without any stressors).To observe changes in the depression-like behavior phenotype during chronic unpredictable mild stress-induced depression,a sucrose preference test was used to evaluate the degree of *** open-field test was used to evaluate locomotor activity and anxiety *** with the control group,chronic unpredictable mild stress rats lost weight but did not have a depression-like behavioral phenotype at 1-4 *** unpredictable mild stress rats presented decreased sucrose preference and locomotor activity at 5-8 *** addition,chronic unpredictable mild stress rats did not have significant anxiety-like behavior during 1-8 weeks of *** observe neurogenesis dysfunctions and changes in neuronal number in the dentate gyrus during chronic unpredictable mild stress-induced depression,markers(DCX and DCX/BrdU)of neural proliferation and differentiation and the neuronal marker NeuN were assessed by *** with the control group,neurogenesis and

关键词： chronic unpredictable mild stress model continuous observation depression depression-like behavior dynamic changes hippocampus miR-124 neurogenesis dysfunction neuronal loss

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Mapping CD20 molecules on the lymphoma cell surface using atomic force microscopy

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Chinese Science Bulletin 2013年第13期58卷 1516-1519页

作者： LI Mi LIU LianQing xi ning WANG YueChao DONG ZaiLi xiAO xiuBin ZHANG WeiJing State Key Laboratory of Robotics Shenyang Institute of AutomationChinese Academy of Sciences University of Chinese Academy of Sciences Department of Mechanical and Biomedical Engineering City University of Hong Kong Department of Lymphoma Affiliated Hospital of Military Medical Academy of Sciences

Atomic force microscopy (AFM) was used to locate CD20 molecules on the surface of lymphoma Raji cells. Rituximab (a monoclonal antibody against CD20) molecules were linked onto the AFM tip via a polyethylene glycol (PEG) linker. Raji cells were adsorbed onto glass slides coated with poly-L-lysine. First, the CD20 distribution in a local area of the cell surface was visualized using the AFM lift scan mode. Second, 16 × 16 force curves were obtained from the same cell area to construct the CD20-rituximab binding force map. Finally, free rituximab was added to block the CD20 molecules on the cell surface and the lift phase image and CD20-rituximab force map were obtained again. The experimental results indicated that when the lift height was greater than the length of the PEG linker, no recognition sites were observed in the lift phase image. However, as the lift height decreased to the length of the PEG linker, some recognition sites were observed in the lift phase image and these sites were generally consistent with the pixels in the force map. After blocking, both the recognition sites in the lift phase image and the gray pixels in the binding force map decreased markedly. These results can improve our understanding of the distribution of protein molecules on the cell surface and facilitate further investigations into cellular functions.

关键词：原子力显微镜细胞表面淋巴瘤分子测绘识别位点单克隆抗体 L-赖氨酸

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An Improvement on Synthesis of Yttrium Isopropoxide

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Journal of Wuhan University of Technology(Materials Science) 2002年第1期17卷 76-78页

作者：陈永熙 WuhanUniversityofTechnology

Yttrium isopropoxide was prepared directly by the reaction between yttrium and isopropyl alcohol using mercuric chloride and iodine as catalyst. Yield is above 83% . This method possesses the merits of easy operational approach, high product purity, high reaction rate and high production rate. Compared with the previous reported results , the period needed was shortened by 19 h and the yieid increased by 8% . The period needed for preparation is about 5 hows. These results are better than the present report . The dehydrolysis method of isopropyl alcohol and the composition of catalyst were presented.

关键词： yttrium isopropoxide yttrium isopropyl alcohol

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Prey decline leads to diet shift in the largest population of Indo-Pacific humpback dolphins?

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Integrative Zoology 2021年第4期16卷 548-574页

作者： Wenzhi LIN Leszek KARCZMARSKI Ruilian ZHOU Yaqian MO Lang GUO Sam King Fung YIU xi ning Tak-Cheung WAI Yuping WU School of Marine Sciences Zhuhai Key Laboratory of Marine Bioresources and EnvironmentGuangdong Provincial Key Laboratory of Marine Resources and Coastal EngineeringSouthern Marine Science and Engineering Guangdong LaboratorySun Yat-Sen UniversityZhuhaiChina Division of Cetacean Ecology Cetacea Research InstituteLantauHong Kong SARChina Marine Mammal and Marine Bioacoustics Laboratory Institute of Deep-sea Science and EngineeringChinese Academy of SciencesSanyaChina School of Biological Sciences University of Hong KongPokfulamHong Kong SARChina State Key Laboratory of Marine Pollution City University of Hong KongHong Kong SARChina

The Pearl River Delta(PRD)region on the southeast coast of China has long been known as a highly productive fishing *** the late 1980s,fishing pressure in the PRD has been intense,which warrants concerns of potential fishery-related impacts on the food resources and foraging ecology of apex marine predators in this region,such as the Indo-Pacific humpback dolphin(Sousa chinensis).In this study,we examined 54 stomachs with food remains,collected from beached carcasses of humpback dolphins recovered during fifteen years between 2003 and *** 6043 identified prey items represent 62 teleost taxa,primarily small estuarine fish,but also larger reef *** dolphins appear to be opportunistic foragers,hunting across the water-column,with preference for shoaling and meaty fishes(*** lucidus IRI%=38.6%,Johnius belangerii IRI%=23.1%,Mugil cephalus IRI%=14.0%).Our findings suggest a dietary shift in recent years,from primarily demersal(as previously reported)to greater intake of neritic and pelagic *** foraging group size has decreased in recent years,which corresponds with declining size and numbers of prey items retrieved from dolphin *** suggest that these are indicators of declining food *** with a shortage of preferred prey,humpback dolphins may have broadened their dietary spectrum to maintain their daily energy intake,while their foraging group size decreased in response to the altered tradeoff between the costs and benefits of group foraging.

关键词： feeding ecology Pearl River Delta prey decline Sousa chinensis stomach content analysis

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Dispatching mobile Agents for DDM applications

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智能系统学报 2008年第2期3卷 181-187页

作者： LI xi-ning Guillaume Autran Department of Computing and Information Science University of Guelph Guelph Ontario N1G 2WlCanada StateKey Laboratory of Novel Software Technology Nanjing University Nanjing 210093China

Techniques for mining information from distributed data sources accessible over the Internet are a growing area of *** mobile Agent paradigm opens a new door for distributed data mining and knowledge discovery *** this paper we present the design of a mobile agent system which couples service discovery,using a logical language based application programming interface,and database *** mobility with database access provides a means to create more efficient data mining *** processing of data is moved to network wide data locations instead of the traditional approach of bringing huge amount of data to the processing *** proposal aims at implementing system tools that will enable intelligent mobile Agents to roam the Internet searching for distributed data *** access the data,discover patterns,extract useful information from facts recorded in the databases,then communicate local results back to the *** user then generates a global data model through the aggregation of results provided by all *** overcomes barriers posed by network congestion,poor security,and unreliability.

关键词：移动主体 DDM 应用软件数据处理

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细胞重编程:调节关键基因获得需要细胞

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中国医学科学院学报 2011年第6期33卷 689-695页

作者：庞希宁中国医科大学细胞生物学卫生部重点实验室干细胞与再生医学研究室沈阳110001

细胞重编程是在一定条件下成体细胞的记忆被擦除,重新程序化产生新的表型和功能,导致细胞命运发生改变,主要发生在不涉及基因组DNA序列改变的基因表达水平,即通过改变细胞基因表达程序(时空和差异),对其进行重新编程而改变细胞的分化方... 详细信息

细胞重编程是在一定条件下成体细胞的记忆被擦除,重新程序化产生新的表型和功能,导致细胞命运发生改变,主要发生在不涉及基因组DNA序列改变的基因表达水平,即通过改变细胞基因表达程序(时空和差异),对其进行重新编程而改变细胞的分化方向,获得所需要的细胞。对于细胞重编程的深入研究有助于掌握机体细胞的发生发育机制,为解决再生医学种子细胞来源问题提供理论基础。人类未来将从许多分化的细胞获得更多所需要的另外一些分化细胞,这对细胞分化机制和疾病的细胞替代治疗研究具有划时代的意义,本文总结了细胞重编程的分类、影响因素、方法及最新进展。

关键词：细胞重编程基因表达去分化转分化

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Exploration of the mechanism by which icariin modulates hippocampal neurogenesis in a rat model of depression

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Neural Regeneration Research 2022年第3期17卷 632-642页

作者： ning-xi Zeng Hui-Zhen Li Han-Zhang Wang Kai-Ge Liu xia-Yu Gong Wu-Long Luo Can Yan Li-Li Wu Research Center for Basic Integrative Medicine Guangzhou University of Chinese MedicineGuangzhouGuangdong ProvinceChina

Icariin(ICA) has a significant capacity to protect against depression and hippocampal injury,but it cannot effectively cross the bloodbrain barrier and accumulate in the ***,the mechanism by which ICA protects against hippocampal injury in depression remains *** this study,we performed proteomics analysis of cerebrospinal fluid to investigate the mechanism by which ICA prevents dysfunctional hippocampal neurogenesis in depression.A rat model of depression was established through exposure to chronic unpredictable mild stress for 6 weeks,after which 120 mg/kg ICA was administered subcutaneously every *** results showed that ICA alleviated depressive symptoms,learning and memory dysfunction,dysfunctional neurogenesis,and neuronal loss in the dentate gyrus of rats with *** stem cells from rat embryonic hippocampi were cultured in media containing 20% cerebrospinal fluid from each group of rats and then treated with 100 μM *** addition of cerebrospinal fluid from rats treated with ICA largely prevented the corticosterone-mediated inhibition of neuronal proliferation and ***-two differentially expressed proteins regulated by chronic unpredictable mild stress and ICA were identified through proteomics analysis of cerebrospinal *** proteins were mainly involved in the ribosome,PI3 K-Akt signaling,and interleukin-17 signaling *** reaction monitoring mass spectrometry showed that Rps4 x,Rps12,Rps14,Rps19,Hsp90 b1,and Hsp90 aa1 were up-regulated by chronic unpredictable mild stress and down-regulated by *** contrast,Htr A1 was down-regulated by chronic unpredictable mild stress and up-regulated by *** findings suggest that ICA can prevent depression and dysfunctional hippocampal neurogenesis through regulating the expression of certain proteins found in the cerebrospinal *** study was approved by the Experimental Animal Ethics Committee of Guangzhou University of Chinese

关键词： cerebrospinal fluid chronic unpredictable mild stress depression dysfunctional hippocampal neurogenesis hippocampus icariin proteomics ribosome pathway

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Di-electrophoresis assembly and fabrication of SWCNT field-effect transistor

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Chinese Science Bulletin 2009年第5期54卷 4451-4457页

作者： TIAN xiaoJun WANG YueChao YU HaiBo DONG ZaiLi xi ning TUNG Steve State Key Laboratory of Robotics Shenyang Institute of Automation Chinese Academy of Sciences Shenyang 110016 China Department of Mechanical Engineering University of Arkansas Fayetteville AR 72701 USA

In the process of fabricating nano electrical device or system based on single-walled carbon nanotube (SWCNT), the controllable assembly and fabrication of SWCNT field-effect transistor (SWCNT FET) is a key issue. SWCNT FET is the most basic and important component in nano electronics. After microelectrode chip of back-gate FET is designed and fabricated, di-electrophoresis technology is adopted to realize the controllable alignment and assembly of SWCNTs, based on dispersing SWCNT by sodium dodecyl sulphate (SDS) facilitated ultra-sonication technique and removing impurities by centrifugal technique. The experiments of SWCNTs assembly demonstrate that SWCNTs are aligned and assembled uniformly at the microelectrodes gap with the alignment density nearly proportional to di-electrophoresis duration and solution concentration. After the processes of rinsing, drying and improving, metallic SWCNTs among the assembled SWCNTs are burned out and residual SDS is removed, and perfect field-effect performance of SWCNT FET is eventually obtained.

关键词：单壁碳纳米管场效应晶体管可控组装电泳技术现场制作十二烷基硫酸钠电子设备电子元件

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星载InSAR均匀相位构形的空间基线稳定性

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国防科技大学学报 2006年第1期28卷 9-12页

作者：刘磊郗晓宁国防科技大学航天与材料工程学院湖南长沙410073

星载InSAR(干涉式合成孔径雷达)在对地观测任务中具有广泛的应用,其空间基线随卫星编队平台的运动而变化。本文基于卫星编队的构形原理,定义了星载InSAR均匀相位构形。根据干涉测量原理定义了相关基线及其稳定度,研究了不同均匀相位构... 详细信息

星载InSAR(干涉式合成孔径雷达)在对地观测任务中具有广泛的应用,其空间基线随卫星编队平台的运动而变化。本文基于卫星编队的构形原理,定义了星载InSAR均匀相位构形。根据干涉测量原理定义了相关基线及其稳定度,研究了不同均匀相位构形下星载InSAR的基线稳定性。

关键词：卫星编队 InsAR(干涉式合成孔径雷达) 均匀相位基线稳定度

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钢筋-纤维自密实混凝土梁受弯性能与承载力分析

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华南理工大学学报（自然科学版） 2015年第7期43卷 42-49页

作者：宁喜亮丁一宁大连理工大学海岸和近海工程国家重点实验室辽宁大连116024

通过四点弯曲试验得到钢筋－纤维自密实混凝土梁式构件的荷载－跨中挠度曲线、荷载－纵筋应变曲线和破坏形态，对梁式构件的受弯承载力及纤维与钢筋的混杂效应进行了分析。结果表明：钢纤维的加入使钢筋－纤维自密实混凝土梁式构件的抗... 详细信息

通过四点弯曲试验得到钢筋－纤维自密实混凝土梁式构件的荷载－跨中挠度曲线、荷载－纵筋应变曲线和破坏形态，对梁式构件的受弯承载力及纤维与钢筋的混杂效应进行了分析。结果表明：钢纤维的加入使钢筋－纤维自密实混凝土梁式构件的抗弯承载力提高了10％～42％。考虑钢纤维跨越裂缝的传力机理及分布情况提出了钢筋－纤维混凝土梁式构件受弯承载力计算公式，并与ACI 544和CECS 38：2004的公式进行了对比，计算结果表明：文中建议公式计算的受弯承载力与试验结果最为接近，可用于钢筋－纤维自密实混凝土梁式构件的受弯分析与设计。

关键词：钢筋钢纤维自密实混凝土钢筋-纤维自密实混凝土梁受弯承载力

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