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黑翅土白蚁菌圃共生菌对小麦秸秆的生物降解

微生物学报 2024年第9期64卷 3393-3408页

作者：李吴晗黄越余婷沈毅周琪欢陈翔莫建初浙江大学昆虫科学研究所农业农村部作物病虫害分子生物学重点实验室浙江杭州310058 杭州市农业技术推广中心浙江杭州310020 桐庐县农业技术推广中心浙江杭州311500

【目的】探究黑翅土白蚁(Odontotermes formosanus)菌圃共生菌在木质纤维素降解上的应用潜力,增加秸秆降解菌种资源并为秸秆资源产业化利用提供理论依据。【方法】以刚果红染色法、羧甲基纤维素钠平板分离筛选具有木质纤维素降解活性的... 详细信息

【目的】探究黑翅土白蚁(Odontotermes formosanus)菌圃共生菌在木质纤维素降解上的应用潜力,增加秸秆降解菌种资源并为秸秆资源产业化利用提供理论依据。【方法】以刚果红染色法、羧甲基纤维素钠平板分离筛选具有木质纤维素降解活性的黑翅土白蚁菌圃共生菌并测定共生菌产酶情况。在常温液态发酵条件下,评估不同菌种以及复合菌群对小麦(Triticum aestivum L.)秸秆的降解效果,利用傅里叶红外光谱、X射线晶体衍射和冷场扫描电镜分析降解前后小麦秸秆的理化性质。【结果】从黑翅土白蚁菌圃中分离得到具有木质纤维素降解活性的5种真菌和3种细菌,经鉴定为青霉属(Penicillium)2种,紫霉属(Purpureocillium)1种,曲霉属(Aspergillus)1种,弯孢聚壳霉属(Eutypella)1种,芽孢杆菌属(Bacillus)1种,埃希氏菌属(Escherichia)1种,寡养单胞菌属(Stenotrophomonas)1种。经酶活测定和单菌降解试验筛选出4种高效降解菌株[桔青霉(Penicillium citrinum)、红绶曲霉(Aspergillus nomiae)、枯草芽孢杆菌(Bacillus subtilis)、大肠杆菌(Escherichia coli)]组成复合菌群。经常温液态发酵,4种菌组成的复合菌群的综合降解能力最强,在12 d内干物质降解率达到24.35%,纤维素降解率达到47.24%,半纤维素降解率达到35.75%,木质素降解率达到32.72%。降解后小麦秸秆内部化学键和分子间作用力受到破坏,木质纤维素复合结构受到破坏,纤维素结晶度从37.40%降低至32.97%,秸秆表面严重崩解,结构蓬松。【结论】从黑翅土白蚁菌圃中分离得到的桔青霉、红绶曲霉、枯草芽孢杆菌、大肠杆菌所组成的复合菌群对小麦秸秆有着优良的降解效果,具备秸秆生物降解产业化的潜在开发价值。

关键词：生物降解黑翅土白蚁共生菌木质纤维素秸秆资源利用

Embryo-chondrocyte expressed gene 1,downregulating hypoxiainducible factor 1α,is another marker of lung tumor hypoxia

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Acta Pharmacologica Sinica 2007年第4期28卷 549-558页

作者： Li ZHANG Qing-quan LI Department of Respiratory Disease,Renmin Hospital,wuhan University,wuhan 430060,China Department of Respiratory Disease Renmin Hospital Wuhan University Wuhan China

Aim:To explore the mechanism of differentiated embryo-chondrocyte expressedgene 1 (DEC 1) in the lung adenocarcinoma A549 cell line and its relationship withhypoxia-inducible factor 1α(HIF-1α).Methods:DEC 1 and HIF-1α protein levelswere detected in lung adenocarcinoma tissues by immunohistochemistry.A549cells were cultured at *** assay was used to determine the effect of *** apoptotic analysis was determined by flow ***-PCR andimmunocytochemistry were carried out to observe the DEC 1 and HIF-1α mRNAand protein ***-1α mRNA and protein levels were detected in thestably transfected pcDNA-DEC 1+/-A549 cells by RT-PCR and Western ***1 mRNA and protein levels were detected in the stably transfected pcDNA-DEC 1+/-A549 cells with or without H1F-1α antisense oligonucleotide treatmentby RT-PCR and Western ***:DEC1 was specifically located in 40(48.8%) lung adenocarcinoma *** results of the MTT test showed thegrowth of the stably transfected pcDNA-DEC+ A549 cells was higher than thoseof A549 cells and the stably transfected pcDNA-DEC-A549 *** alsoinduced the apoptosis of A549 cells and the stably transfected pcDNA-DEC+/-A549 *** increased the mRNA and protein levels of DEC1 and HIF-1α.Both HIF-1α mRNA and protein levels decreased in the stably transfected pcDNA-DEC+cells,but HIF-1α antisense oligonucleotide had no effect on DEC1 in thestably transfected pcDNA-DEC+/-A549 ***:DEC 1 is a marker oftumor ***1 downregulates the HIF-1α at both mRNA and protein levelsat hypoxia in lung adenocarcinoma A549 cells.

关键词： DECI lung adenocarcinoma hypoxia HIF-1

THE NON-DIFFERENTIABILITY OF A CLASS OFFRACTAL INTERPOLATION FUNCTIONS

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Acta Mathematica Scientia 1999年第4期19卷 425-430页

作者：陈世荣 Department of Physics Wuhan University Wuhan 430072 China

Hardin and Massopust([1]) introduced a class of fractal interpolation functions and calculated their Bouligand dimensions. This paper deals with the non-differentiability of these functions and shows some conditions under which they are nowhere differentiable. The basic technique here is based on the presentation the author obtains.

关键词： fractal interpolation iterated system nowhere differentiablity Hausdorff metric

Metallothionein mediates cardioprotection of isoliquiritigenin against ischemia-reperfusion through JAK2/STAT3 activation

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Acta Pharmacologica Sinica 2006年第11期27卷 1431-1437页

作者： Wei AN Jing YANG~1 Ying AO Department of Pharmacology,School of Medicine,wuhan University,wuhan 430071,China Department of Pharmacology School of Medicine Wuhan University Wuhan China

Aim:To examine whether isoliquiritigenin(ISL)can attenuate myocardial ischemia-reperfusion(MI/R)injury in rats by inducing metallothionein(MT)through acti-vation of janus kinase 2(JAK 2)/signal transducers and activators of transcription3(STAT 3)***:The experimental model of MI/R in rats was gener-ated by 30 min of ischemia and reperfusion for 2 *** mRNA expression of MT,COX-2,and iNOS were measured by *** protein expressions of MT,JAK/STAT,extracellular signal-regulated kinase(ERK),and Akt were determinedby Western blotting in the absence or presence of a JAK kinase inhibitor,tyrphostinAG490(1.0 mg/kg,iv,1h before ischemia).Results:Pretreatment with ISL mark-edly decreased the severity of reperfusion-induced arrhythmias and myocardialinfarct *** the ISL 20 mg/kg group,the activities of lactate dehydrogenase(LDH)and creatinine phosphokinase(CPK)were reduced by 38.4% and 51.3%when compared with the vehicle *** JAK 2/STAT 3 phosphoryla-tion was accompanied by increased synthesis of MT but not of COX-2 or iNOS inISL-treated ***490 can significantly weaken ISL-induced cardioprotectionand prevent the increase of MT expression and JAK 2/STAT 3 ***:ISL protected MI/R injury through activation of JAK 2/STAT 3 sig-nal transduction pathway,which might be involved in mediating the upregulationof MT expression.

关键词： isoliquiritigenin myocardial ischemiareperfusion metallothionein janus kinase STAT3 transcription factors

Kinetic Resolution of DL-Phenylalanine Methyl Ester by α -Chymotrypsin ImmobiIized on Porous Silica Beads

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Chinese Chemical Letters 1997年第5期8卷 413-416页

作者： Li Jian LIU Ping YANG Ren Xi ZHUO(Department of Chemistry, wuhan University, wuhan 430072) Department of Chemistry Wuhan University Wuhan 430072 China

Kinetic resolution of DL-phenylalanine methyl ester was carried out using drimobilized α -chymotrypsin (IC) as catalyst. The effects of temperatUre, pH, concentration of substrate and reactionvessels on the resolutio... 详细信息

关键词： DL Chymotrypsin Immobilized on Porous Silica Beads Kinetic Resolution of DL-Phenylalanine Methyl Ester by

On the Normal Criterion of Quasimeromorphic Mappings

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wuhan University Journal of Natural Sciences 2001年第3期6卷 621-625页

作者： Deng Fang\|wen College of Mathematics and Statistics, wuhan University, wuhan 430072, China College of Mathematics and Statitics Wuhan University Wuhan China

In this paper, we generalize an, inequality of meromorphic mappings to quasimeromorphic ones. Applying the results here, we can establish a normal criterion of quasimeromorphic mappings.

关键词： quasimeromorphic mapping covering surface normal criterion

Synthesis of Polymer-Bonded Quaternary Ammonium Type Metal Porphyrins

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Chinese Chemical Letters 2000年第1期11卷 27-28页

作者： Zao Ying LI Cong LI Xu Ye WU( Department of Chemistry, wuhan University, wuhan 430072) Department of Chemistry Wuhan University Wuhan 43072 China

The reaction of pyrrole with 4-hydroxybenzaldehyde and 4-pyridinecarboxaldehyde afforded new porphyrin ligand 1. Treatment of 1 with copper( II ) acetate, cobalt ( II ) acetate gave complexes 2-3 respectively. 2 and 3 reacted with Merrifield's peptide resin produced 4 and 5. Complexes 4 and 5 reacted with methyl iodide respectively gave 6-7. The new compounds 1-7 have been identified by H-1 NMR, IR, MS and UV-visible spectra, elemental analysis and AES.

关键词： polymer-bonded quaternary ammonium type metal porphyrin synthesis

Tele-Network Design Based on Queue Competition Algorithm

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wuhan University Journal of Natural Sciences 2003年第S1期8卷 327-330页

作者： Huang Zhang-can, Wan Li-jun, Tang Tao, Chen Zheng-xuState Key Laboratory of Software Engineering, wuhan University, wuhan 430072, Hubei, ChinaSchool of Material Science and Engineering, wuhan University of Technology , wuhan 43.070, Hubei, ChinaSchool of Automation, wuhan University of Technology, wuhan 43.070, Hubei, ChinaSchool of Science, wuhan University of Technology, wuhan 43.070, Hubei, China State Key Laboratory of Software Engineering Wuhan University Wuhan Hubei China School of Material Science and Engineering Wuhan University of Technology Wuhan Hubei China School of Automation Wuhan University of Technology Wuhan Hubei China School of Science Wuhan University of Technology Wuhan Hubei China

In this paper, we report research on how to design the tele-network. First of all, we defined the reliability of tele-network. According to the definition, we divide the whole reliability into two parts:the reliability of the mini-way and that of the whole system. Then we do algebra unintersection of the mini-way, deriving a function of reliability of tele-network. Also, we got a function of the cost of tele-network after analyzing the cost of arcs and points. Finally, we give a mathematical model to design a tele-network. For the algorithm, we define the distance of a network and adjacent area within certain boundaries . We present a new algorithm--Queue Competition Algorithm (QCA) based on the adjacent area . The QCA correlates sequence of fitnesses in their father-generations with hunting zone of mutation and the number of individuals generated by mutation, making the stronger fitness in a small zone converge at a local extreme value, but the weaker one takes the advantage of lots of individuals and a big zone to hunt a new local extreme value. In this way, we get the overall extreme value. Numerical simulation shows that we can get the efficient hunting and exact solution by using QCA. The QCA efficient hunting and exact solution.

关键词： reliability Queue Competition algorithm the distance of a network adjacent area

Irregular but Nonfractal Drums and n-Dimensional Weyl Conjecture

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Acta Mathematica Sinica,English Series 1995年第2期11卷 168-178页

作者： Chen Hua Department of Mathematics wuhan University wuhan, 430072 China Department of Mathematics Wuhan University Wuhan People's Republic of China

In this paper, we study the asymptotics of the spectrum of the Dirichlet （or Neumann） Laplacian in a bounded open set ΩRn（n≥1） with irregular but nonfractal boundary Ω. We give a partial resolution of the Weyl conjecture, i.e. for the counting function Ni（λ）（i=0: Dirichlet; i=i: Neumann）, we have got a precise estimate of the remainder term Ψi（λ）=φ（λ）-Ni（λ） for large λ, where φ（λ） is the Weyl term. This implies that for the irregular but nonfractal drum Ω, not only the volume |Ω|n is spectral invariant but also the area of boundary |Ω|n-1 might be spectral invariant as well.

关键词： Math Irregular but Nonfractal Drums and n-Dimensional Weyl Conjecture