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Acta Oceanologica Sinica 2020年第4期39卷 33-41页

作者： Zhongqiao Li Ying wu Liyang Yang Jinzhou Du Bing Deng Jing Zhang State Key Laboratory of Estuarine and Coastal Research East China Normal UniversityShanghai 210012China Key Laboratory of Marine Ecosystem Dynamics Second Institute of OceanographyMinistry of Natural ResourcesHangzhou 310012China College of Environment and Resources Fuzhou UniversityFuzhou 350108China

The Lake Tian E Zhou(TEZ,an oxbow lake)was formed during the rerouting of the Changjiang River in 1972,with strong influences from the main river channel and flood ***,a sediment core was collected from the Lake TEZ for the measurements of carbon isotopes and biomarkers,including stable carbon isotopes(δ13C),radiocarbon composition(?14C),and lignin phenols,as well as lead-210 to reconstruct recent heavy flood events over the past 70 *** the 24–26 cm interval,the sediment contained the highest OC%,TN%,and lignin phenols content,as well as significantly depleted 13C but enriched 14C,corresponding to the extreme flood event in *** addition,statistics from t-test showed that lignin phenols normalized to OC(Λ8),the concentration of 3,5-dihydroxy benzoic acid(3,5-BD),and the ratio of p-hydroxy benzophenone to total hydroxyl phenols(PHB/hp)were all significantly different between the layers containing flood deposits and the layers deposited under normal non-flood conditions(p<0.05).These results indicate that the later three parameters are highly related to flood events and can be used as compelling proxies,along with sediment chronology,for hydrological changes and storm/flood events in the river basin and coastal marine environments.

关键词： flood record carbon isotopes lignin phenols Changjiang River Lake Tian E Zhou

替米沙坦辅助治疗轻中度高血压合并2型糖尿病的效果研究

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中国药物滥用防治杂志 2024年第2期30卷 315-318,324页

作者：肖火金郭云聪伍礼荣福建省三明市将乐县高唐镇中心卫生院福建三明353301 福建省三明市将乐县总医院福建三明353300

目的:探讨轻中度高血压(hp)合并2型糖尿病(T2DM)患者给予替米沙坦联合硝苯地平控释片、二甲双胍治疗的临床效果。方法:选取2021年8月—2022年7月福建省三明市将乐县高唐镇中心卫生院收治的轻中度hp合并T2DM患者共计92例,以随机数字表法... 详细信息

目的:探讨轻中度高血压(hp)合并2型糖尿病(T2DM)患者给予替米沙坦联合硝苯地平控释片、二甲双胍治疗的临床效果。方法:选取2021年8月—2022年7月福建省三明市将乐县高唐镇中心卫生院收治的轻中度hp合并T2DM患者共计92例,以随机数字表法分成研究组(46例)与对照组(46例)。对照组给予硝苯地平控释片、二甲双胍治疗,研究组给予替米沙坦联合硝苯地平控释片、二甲双胍治疗,比较两组临床疗效、血压水平[收缩压(SBP)、舒张压(DBP)]、血糖水平[空腹血糖(FPG)、糖化血红蛋白(HbA1c)、餐后2h血糖(2hpG)]、血脂水平[高密度脂蛋白(HDL)、低密度脂蛋白(LDL)、甘油三酯(TG)]及不良反应。结果:研究组治疗总有效率为91.30%,高于对照组的76.09%(P0.05)。结论:轻中度hp合并T2DM患者给予替米沙坦联合硝苯地平控释片、二甲双胍治疗能够提高临床疗效,调节患者血压、血糖及血脂水平,具有较高的安全性。

关键词：轻中度高血压 2型糖尿病替米沙坦硝苯地平控释片二甲双胍

Wet adhesive hydrogel cardiac patch loaded with anti-oxidative,autophagy-regulating molecule capsules and MSCs for restoring infarcted myocardium

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Bioactive Materials 2023年第3期21卷 20-31页

作者： Tengling wu Xiaoping Zhang Yang Liu Chunyan Cui Yage Sun Wenguang Liu School of Materials Science and Engineering Tianjin Key Laboratory of Composite and Functional MaterialsTianjin UniversityTianjin300350China

Hydrogel patch-based stem cell transplantation and microenvironment-regulating drug delivery strategy is promising for the treatment of myocardial infarction(MI).However,the low retention of cells and drugs limits their therapeutic ***,we propose a prefixed sponge carpet strategy,that is,aldehyde-dextran sponge(ODS)loading anti-oxidative/autophagy-regulating molecular capsules of 2-hydroxy-β-cyclodextrin@resveratrol(hp-β-CD@Res)is first bonded to the rat’s heart via capillary removal of interfacial water from the tissue surface,and the subsequent Schiff base reaction between the aldehyde groups on ODS and amino groups on myocardium ***,an aqueous biocompatible hydrazided hyaluronic acid(HHA)solution encapsulating mesenchymal stem cells(MSCs)is impregnated into the anchored carpet to form HHA@ODS@hp-β-CD@Res hydrogel in situ via click reaction,thus prolonging the in vivo retention time of therapeutic drug and ***,the HHA added to outer surface consumes the remaining aldehydes to contribute to nonsticky top surface,avoiding adhesion to other *** embedded hp-β-CD@Res molecular capsules with antioxidant and autophagy regulation bioactivities can considerably improve cardiac microenvironment,reduce cardiomyocyte apoptosis,and enhance the survival of transplanted MSCs,thereby promoting cardiac repair by facilitating angiogenesis and reducing cardiac fibrosis.

关键词： Myocardial infarction Sponge Hydrogel patch Wet adhesive Autophagy

Proteolytic processing of SDF-1α by matrix metalloproteinase-2 impairs CXCR4 signaling and reduces neural progenitor cell migration

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Protein & Cell 2012年第11期3卷 875-882页

作者： Hui Peng Yumei wu Zhiyuan Duan Pawel Ciborowski Jialin C.Zheng Laboratory of Neuroimmunology and Regenerative Therapy University of Nebraska Medical CenterOmahaNE 68198USA Departments of Pharmacology and Experimental Neuroscience University of Nebraska Medical CenterOmahaNE 68198USA Departments of Pathology and Microbiology University of Nebraska Medical CenterOmahaNE 68198USA Institute of Genetics and Developmental Biology Chinese Academy of SciencesBeijing 100101China

Neural stem cells and neural progenitor cells(NPCs)exist throughout life and are mobilized to replace neurons,astrocytes and oligodendrocytes after ***-omal cell-derived factor 1(SDF-1,now named CXCL12)and its receptor CXCR4,an α-chemokine receptor,are critical for NPC migration into damaged areas of the *** previous studies demonstrated that immune activated and/or HIV-1-infected human monocyte-derived-macrophages(MDMs)induced a substantial increase of SDF-1 production by human ***,matrix metalloproteinase(MMP)-2,a protein up-regulated in HIV-1-infected macrophages,is able to cleave four amino acids from the N-terminus of SDF-1,resulting in a truncated SDF-1(5-67).In this study,we investigate the diverse signaling and function induced by SDF-1α and SDF-1(5-67)in human cortical ***-1(5-67)was generated by incubating human recombinant SDF-1α with MMP-2 followed by protein determination via mass spectrometry,Western blotting and ***-1α induced time-dependent phosphorylation of extracellular signal-regulated kinases(ERK)1/2,Akt-1,and diminished cyclic adenosine monophosphate(cAMP).In contrast,SDF-1(5-67)failed to induce these ***-1α activation of CXCR4 induced migration of NPCs,an effect that is dependent on ERK1/2 and Akt-1 pathways;whereas SDF-1(5-67)failed to induce NPC *** observation provides evidence that MMP-2 may affect NPC migration through post-translational processing of SDF-1α.

关键词： proteolysis chemokine neurogenesis and migration

One stone two birds: Biosynthesis of 3-hydroxypropionic acid from CO2 and syngas-derived acetic acid in Escherichia coli

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Synthetic and Systems Biotechnology 2021年第3期6卷 144-152页

作者： Ningyu Lai Yuanchan Luo Peng Fei Peng Hu Hui wu State Key Laboratory of Bioreactor Engineering School of BiotechnologyEast China University of Science and Technology130 Meilong RoadShanghai200237China Shanghai Collaborative Innovation Center for Biomanufacturing Technology 130 Meilong RoadShanghai200237China Key Laboratory of Bio-based Material Engineering of China National Light Industry Council 130 Meilong RoadShanghai200237China Shanghai GTL Biotech Co. Ltd.1688 North Guoquan RoadShanghai200438China

Syngas,which contains large amount of CO2 as well as H2 and CO,can be convert to acetic acid chemically or ***,acetic acid become a cost-effective nonfood-based carbon source for value-added biochemical *** this study,acetic acid and CO2 were used as substrates for the biosynthesis of 3-hydroxypropionic acid(3-hp)in metabolically engineered Escherichia coli carrying heterogeneous acetyl-CoA carboxylase(Acc)from Corynebacterium glutamicum and codon-optimized malonyl-CoA reductase(MCR)from Chloroflexus *** of metabolic engineering included promoting glyoxylate shunt pathway,inhibiting fatty acid synthesis,dynamic regulating of TCA cycle,and enhancing the assimilation of acetic *** engineered strain LNY07(M*DA)accumulated 15.8 g/L of 3-hp with the yield of 0.71 g/g in 48 h by whole-cell ***,syngas-derived acetic acid was used as substrate instead of pure acetic *** concentration of 3-hp reached 11.2 g/L with the yield of 0.55 g/g in LNY07(M*DA).The results could potentially contribute to the future development of an industrial bioprocess of 3-hp production from syngas-derived acetic acid.

关键词： Syngas-derived acetic acid 3-Hydroxypropionic acid Metabolic engineering Escherichia coli Dynamic regulation

TEM and HRTEM Study of Microstructures in Omphacite from Uhp Eclogites at Shima,Dabie Mountains,China

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Acta Geologica Sinica(English Edition) 2000年第2期74卷 154-162页

作者： wu Xiuling HAN Yujing MENG Dawei YANG Weiran LI Douxing Test Center and Faculty of Earth Sciences China University of GeosciencesWuhan 430074Hubei Laboratory of Atomic Imaging of Solids Institute of Metal ResearchChinese Academy of SciencesShenyang 110015Liaoning

Transmission electron microscope (TEM) and high resolution transmission electron microscope (HRTEM) analyses have been performed on omphacite from ultra-high pressure (Uhp) eclogites at the locality of Shima, Dabie Mountains, China. TEM reveals that the microstructures consist dominantly of dislocation substructures, including free dislocations, loops, tiltwalls, dislocation tangles and subboundaries. They were produced by high-temperature ductile deformation, of which the main mechanism was dislocation creep. Antiphase domain (APD) boundaries are common planar defects; an age of 470 ± 6 Ma for Uhp eclogite formation has been obtained from the equiaxial size of APDs in ordered omphacites from Shima, coincident with ages given by single-zircon U-Pb dating (471 ± 2 Ma). HRTEM reveals C2/c and P2/n space groups in different parts of one single omphacite crystal, and no exsolution is observed in the studied samples, which is attributed to rapid cooling. It is suggested that the Uhp eclogites underwent a long period of annealing at high temperatures, followed by relatively rapid cooling. These data provide valuable information for the formation and exhumation mechanism of Uhp eclogites in the Dabie high-pressure (hp) and Uhp metamorphic belt.

关键词： Uhp eclogite omphacite TEM and HRTEM Dabie Mountains

马占相思幼苗不同器官非结构性碳水化合物含量对磷添加的响应

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应用与环境生物学报 2024年第2期30卷 247-253页

作者：吴妙兰官滢滢王艺颖刘悦何至杭张少纯曾睿楷莫其锋华南农业大学林学与风景园林学院广东省森林植物种质创新与利用重点实验室广州510642 中国森林生态系统定位观测研究网络广东鹅凰嶂野外科学观测研究站阳江529631

非结构性碳水化合物(NSC)在植物抵御生物和非生物胁迫时发挥着不可或缺的作用.以马占相思作为研究对象,设置对照(CK,不加磷)、低磷(LP,+50 kg hm^(-2)a^(-1))、高磷(hp,+100 kg hm^(-2)a^(-1))3种处理,探究不同浓度磷添加对马占相思幼... 详细信息

非结构性碳水化合物(NSC)在植物抵御生物和非生物胁迫时发挥着不可或缺的作用.以马占相思作为研究对象,设置对照(CK,不加磷)、低磷(LP,+50 kg hm^(-2)a^(-1))、高磷(hp,+100 kg hm^(-2)a^(-1))3种处理,探究不同浓度磷添加对马占相思幼苗各器官的NSC含量及分配格局的影响.结果表明:(1)低磷(LP)处理显著增加马占相思幼苗苗高和地径的相对生长速率(RGR);苗高、地径的RGR在不同处理下均表现为LP>hp>CK.低磷和高磷均显著增加植株的总生物量;但是低磷显著提高了叶、枝、茎和粗根的生物量占全株生物量的比例,高磷仅改变了粗根的生物量占比.(2)磷添加显著增加叶绿素a、叶绿素b和总叶绿素浓度,具体表现为hp>LP>CK,(3)与CK相比,LP和hp处理下枝的NSC含量显著降低;粗根的NSC含量在LP处理下显著低于CK和hp处理.磷添加显著增加粗根可溶性糖占整株的百分比,同时磷添加显著增加枝、茎和粗根淀粉与NSC占整株含量的百分比.本研究表明低磷添加显著促进马占相思幼苗的生长,并通过调整NSC在不同器官的分配比例来应对土壤磷供应水平,以更好地适应土壤低磷环境.(图4表3参46)

关键词：非结构性碳磷添加分配格局马占相思人工林华南

胃功能三项及NLR、CEA联合检测对胃溃疡良恶性的诊断价值分析

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分子诊断与治疗杂志 2024年第3期16卷 485-489页

作者：张磊吴军李宏伟程磊陈筠安徽省淮南市朝阳医院老年医学科安徽淮南232000 安徽省淮南市朝阳医院消化内科安徽淮南232000

目的分析研究胃功能三项[胃蛋白酶原Ⅰ(PGⅠ)、胃蛋白酶原Ⅱ(PGⅡ)、胃蛋白酶原比值(PGR)]及中性粒细胞/淋巴细胞比值(NLR)、癌胚抗原(CEA)联合检测对胃溃疡良恶性的诊断价值。方法选取2020年1月至2022年11月淮南朝阳医院收治的胃溃... 详细信息

目的分析研究胃功能三项[胃蛋白酶原Ⅰ(PGⅠ)、胃蛋白酶原Ⅱ(PGⅡ)、胃蛋白酶原比值(PGR)]及中性粒细胞/淋巴细胞比值(NLR)、癌胚抗原(CEA)联合检测对胃溃疡良恶性的诊断价值。方法选取2020年1月至2022年11月淮南朝阳医院收治的胃溃疡患者为研究对象,将其命名为胃溃疡组(n=100),另选同期在本院体检的健康人群为对照组(n=60)。比较两组外周血PGⅠ、PGⅡ、PGR、NLR及CEA水平;根据内镜与病理检查结果,将胃溃疡组患者分为良性组(n=76)与恶性组(n=24)。采用多因素logistics回归分析胃溃疡恶性病变的影响因素;绘制ROC曲线分析外周血PGⅠ、PGⅡ、PGR、NLR、CEA对胃溃疡恶性病变的预测价值。结果胃溃疡组的PGⅠ、PGR水平均低于对照组,差异均具有统计学意义(t=22.044、35.045,Php感染阳性(OR=1.865)、过量饮酒(OR=2.046)、PGⅠ降低(OR=2.006)、PGⅡ升高(OR=2.028)、PGR降低(OR=2.098)、NLR升高(OR=1.958)以及CEA水平升高(OR=2.071)均是胃溃疡恶性病变的独立危险因素(P<0.05);ROC曲线分析显示,PGⅠ、PGⅡ、PGR、NLR、CEA及联合检测预测胃溃疡良恶性病变的曲线下面积(AUC)分别为0.822、0.815、0.863、0.786、0.828、0.951,联合检测优于单一检测(P<0.05)。结论 PGⅠ、PGⅡ、PGR及NLR、CEA联合检测对胃溃疡良恶性病变具有一定的诊断价值。

关键词：胃溃疡良恶性胃功能三项中性粒细胞/淋巴细胞比值癌胚抗原

Shp-2对人绒毛膜滋养层细胞增殖、侵袭、迁移和PI3K/AKT信号通路蛋白表达的影响

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西部医学 2024年第5期36卷 636-641页

作者：张秀群伍欣覃婷蒙明慧广西壮族自治区人民医院医学遗传与产前诊断中心广西南宁530021

目的探讨Src同源物2磷酸酶2(Shp-2)对人绒毛膜滋养层细胞HTR-8/SVneo增殖、侵袭、迁移和PI3K/AKT信号通路蛋白表达的影响。方法用定量实时聚合酶链反应(qRT-PCR)检测Shp-2在子痫前期(PE)组胎盘组织中的表达水平。采用细胞计数试剂盒-8(C... 详细信息

目的探讨Src同源物2磷酸酶2(Shp-2)对人绒毛膜滋养层细胞HTR-8/SVneo增殖、侵袭、迁移和PI3K/AKT信号通路蛋白表达的影响。方法用定量实时聚合酶链反应(qRT-PCR)检测Shp-2在子痫前期(PE)组胎盘组织中的表达水平。采用细胞计数试剂盒-8(CCK-8)、克隆形成实验、划痕闭合和Transwell分析检测敲低或过表达Shp-2对人绒毛膜滋养细胞(HTR-8/SVneo)增殖、集落形成、迁移和侵袭能力的影响。Western blot检测侵袭相关蛋白的表达和磷脂酰肌醇3激酶(PI3K)、蛋白激酶B(AKT)的磷酸化水平。结果hp-2在PE组胚胎组织中低表达。敲低Shp-2显著抑制HTR-8/SVneo细胞的增殖、集落形成、侵袭和迁移,此外,基质金属蛋白酶-2(MMP-2)、N-钙黏蛋白(N-cadherin)和波形蛋白(Vimentin)的表达显著下调,E-钙黏蛋白(E-cadherin)表达显著上调,PI3K和AKT的磷酸化水平显著降低,然而,过表达Shp-2具有相反的效果。结论Shp-2在PE胎盘组织中低表达,Shp-2过表达可促进滋养层细胞的增殖、侵袭和迁移能力,提示Shp-2可能是临床治疗PE的潜在靶点。

关键词： Src同源物2磷酸酶2 人绒毛膜滋养层细胞增殖侵袭迁移

肉桂醛调节Shh/Gli1信号通路对幽门螺旋杆菌胃炎大鼠胃黏膜损伤的影响

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安徽医学 2024年第2期45卷 135-141页

作者：黄方黄莉璇吴彬李名福卢长江广西中医药大学研究生院广西南宁530001 南宁市妇幼保健院广西南宁530001 广西中医药大学赛恩斯新医药学院广西南宁530001

目的探讨肉桂醛调节音猬因子(Shh)/Gli家族锌指蛋白1(Gli1)信号通路对幽门螺旋杆菌(hp)胃炎大鼠胃黏膜损伤的影响。方法将大鼠随机分为对照组、模型组、肉桂醛低剂量组、肉桂醛中剂量组、肉桂醛高剂量组、替普瑞酮组、肉桂醛高剂量+Sh... 详细信息

目的探讨肉桂醛调节音猬因子(Shh)/Gli家族锌指蛋白1(Gli1)信号通路对幽门螺旋杆菌(hp)胃炎大鼠胃黏膜损伤的影响。方法将大鼠随机分为对照组、模型组、肉桂醛低剂量组、肉桂醛中剂量组、肉桂醛高剂量组、替普瑞酮组、肉桂醛高剂量+Shh激活剂Purmorphamine(PUR)组,每组18只。通过灌胃hp的方法构建胃炎大鼠模型。建模成功后,肉桂醛低、中、高剂量组大鼠分别灌胃12.5 mg/kg、25 mg/kg、50 mg/kg肉桂醛,替普瑞酮组大鼠灌胃0.13 g/kg替普瑞酮,肉桂醛高剂量+PUR组大鼠给予50 mg/kg肉桂醛灌胃和6.67 mg/kg PUR腹腔注射,每天给药1次,持续2周。HE染色检测大鼠胃黏膜组织病理变化;透射电镜观察大鼠胃黏膜细胞形态。将GES-1细胞分为vitro-对照组、vitro-模型组、vitro-肉桂醛低剂量组、vitro-肉桂醛中剂量组、vitro-肉桂醛高剂量组、vitro-替普瑞酮组、vitro-肉桂醛高剂量+PUR组。除vitro-对照组外,其他组GES-1细胞均需被hp感染,感染结束后,vitro-肉桂醛低剂量组、vitro-肉桂醛中剂量组、vitro-肉桂醛高剂量组分别用5μmol/L、10μmol/L、20μmol/L肉桂醛处理24 h;vitro-替普瑞酮组用1μmol/L替普瑞酮处理24 h;vitro-肉桂醛高剂量+PUR组用20μmol/L肉桂醛和1μmol/L PUR共同处理24 h,CCK-8法检测GES-1细胞增殖抑制率;ELISA法检测大鼠胃黏膜组织及GES-1细胞上清中白细胞介素1β (IL-1β)、肿瘤坏死因子-α(TNF-α)水平;Western blot检测大鼠胃黏膜组织及GES-1细胞中Shh、Gli1蛋白表达。结果与对照组比较,模型组大鼠胃黏膜组织病理损伤严重,胃黏膜表面上皮细胞固缩,细胞膜破损,IL-1β、TNF-α水平及Shh、Gli1蛋白表达升高(Php诱导的胃炎大鼠胃黏膜损伤。

关键词：肉桂醛幽门螺旋杆菌 Shh/Gli家族锌指蛋白1信号通路胃炎炎症