One stone two birds: Biosynthesis of 3-hydroxypropionic acid from CO2 and syngas-derived acetic acid in Escherichia coli

作     者：Ningyu Lai Yuanchan Luo Peng Fei Peng Hu Hui Wu 

作者机构：State Key Laboratory of Bioreactor EngineeringSchool of BiotechnologyEast China University of Science and Technology130 Meilong RoadShanghai200237China Shanghai Collaborative Innovation Center for Biomanufacturing Technology130 Meilong RoadShanghai200237China Key Laboratory of Bio-based Material Engineering of China National Light Industry Council130 Meilong RoadShanghai200237China Shanghai GTL Biotech Co.Ltd.1688 North Guoquan RoadShanghai200438China 

出 版 物：《Synthetic and Systems Biotechnology》 (合成和系统生物技术（英文）)

年 卷 期：2021年第6卷第3期

页      面：144-152页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：supported by the Natural Science Foundation of Shanghai(19ZR1472700) the Fok Ying-Tong Education Foundation,China(Grant No.161017) the National Natural Science Foundation of China(Grant No.21776083) the Fundamental Research Funds for the Central Universities(Grant No.22221818014) supported by Open Funding Project of the CAS Key Laboratory of Synthetic Biology 

主　　题：Syngas-derived acetic acid 3-Hydroxypropionic acid Metabolic engineering Escherichia coli Dynamic regulation 

摘      要：Syngas,which contains large amount of CO2 as well as H2 and CO,can be convert to acetic acid chemically or ***,acetic acid become a cost-effective nonfood-based carbon source for value-added biochemical *** this study,acetic acid and CO2 were used as substrates for the biosynthesis of 3-hydroxypropionic acid(3-HP)in metabolically engineered Escherichia coli carrying heterogeneous acetyl-CoA carboxylase(Acc)from Corynebacterium glutamicum and codon-optimized malonyl-CoA reductase(MCR)from Chloroflexus *** of metabolic engineering included promoting glyoxylate shunt pathway,inhibiting fatty acid synthesis,dynamic regulating of TCA cycle,and enhancing the assimilation of acetic *** engineered strain LNY07(M*DA)accumulated 15.8 g/L of 3-HP with the yield of 0.71 g/g in 48 h by whole-cell ***,syngas-derived acetic acid was used as substrate instead of pure acetic *** concentration of 3-HP reached 11.2 g/L with the yield of 0.55 g/g in LNY07(M*DA).The results could potentially contribute to the future development of an industrial bioprocess of 3-HP production from syngas-derived acetic acid.