检索结果-南通市图书馆

Efficient Self-Assembled DNA Nanoparticles through Rolling Circle Amplification for siRNA Delivery in vitro

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Chemistry 2019年第6期37卷 588-592页

作者： Qian Yao Yuqi Chen fan wu1 fan wu Chaoxing Liu Tingting Hong Wei Li Yi Chen Xiang Zhou College of Chemistry and Molecular Sciences Key Laboratory of Biomedical Polymers of Ministry of EducationHubei Province Key Laboratory of Allergy and ImmunologyWuhan UniversityWuhanHubei 430072China

Effective and low toxicity delivery of siRNA is of great importance for clinical gene therapy. Herein, self-assembled DNA nanoparticles (NPs) based on rolling circle amplification (RCA) with a small interfering RNA (siRNA) payload were successfully developed as a facile and efficient siRNA delivery strategy. This intracellular gene silencing strategy exhibits various advantages including low toxicity, high efficiency, and good stability. The synthesized DNA NPs serve as siRNA carriers, protecting the siRNA against nuclease degradation. We demonstrate that the obtained self-assembled siRNA/NP/PEI system can successfully deliver enhanced green fluorescent protein (EGFP)-siRNA into HeLa cells, realizing the same EGFP knockdown efficiency with less toxicity as that of commercial Lipofectamine 2000.

关键词： siRNA delivery self-assembly RCA DNA nanopartides gene silencing

Anisotropic density estimation for photon mapping

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Computational Visual Media 2015年第3期1卷 221-228页

作者： Fu-Jun Luan Li-fan wu Kun Xu TNList Department of Computer Science and Technology Tsinghua University

Photon mapping is a widely used technique for global illumination rendering. In the density estimation step of photon mapping, the indirect radiance at a shading point is estimated through a filtering process using nearby stored photons; an isotropic filtering kernel is usually used. However,using an isotropic kernel is not always the optimal choice, especially for cases when eye paths intersect with surfaces with anisotropic BRDFs. In this paper,we propose an anisotropic filtering kernel for density estimation to handle such anisotropic eye *** anisotropic filtering kernel is derived from the recently introduced anisotropic spherical Gaussian representation of BRDFs. Compared to conventional photon mapping, our method is able to reduce rendering errors with negligible additional cost when rendering scenes containing anisotropic BRDFs.

关键词： photon mapping density estimation anisotropic anisotropic spherical Gaussian

Air/Water Stability Problems and Solutions for Lithium Batteries

在线全文

学校读者我要写书评

暂无评论

Energy Material Advances 2022年第1期2022卷 165-205页

作者： Ming Yang Liquan Chen Hong Li fan wu Tianmu Lake Institute of Advanced Energy Storage Technologies Liyang213300 JiangsuChina Nano Science and Technology Institute University of Science and Technology of ChinaSuzhou 215123China Yangtze River Delta Physics Research Center Liyang213300 JiangsuChina Beijing Advanced Innovation Center for Materials Genome Engineering Key Laboratory for Renewable EnergyBeijing Key Laboratory for New Energy Materials and DevicesInstitute of PhysicsChinese Academy of SciencesBeijing 100190China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China

Recently,lithium-ion batteries(LIBs)have faced bottlenecks in terms of energy/power density and safety issues caused by flammable *** this regard,all-solid-state batteries(ASSBs)may be one of the most promising ***,many key battery materials(such as solid electrolytes(SEs),cathodes,and anodes)are unstable to air/water,which greatly limits their production,storage,transportation,practical applications,and the development of ***,the research status on air/water stability of SEs,cathodes,and anodes is *** mechanisms for their air/water instability are revealed in *** corresponding modification methods are also proposed,with emphasis on the construction strategies of air/water stable protective layers,including ex situ coatings and in situ ***,the application of air/water-stable protective layers in ASSBs is discussed *** but not least,the advantages and disadvantages of various protective layer construction strategies are analyzed,in which their applications in practical production are prospected.

关键词： battery protective details

Automatic recognition of loess landforms using Random Forest method

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Journal of Mountain Science 2017年第5期14卷 885-897页

作者： ZHAO wu-fan XIONG Li-yang DING Hu TANG Guo-an Key Laboratory of Virtual Geographic Environment (Nanjing Normal University) Ministry of Education Nanjing 210023 China Jiangsu Center for Collaborative Innovation in Geographical Information Resource Development and Application Nanjing 210023 China State Key Laboratory Cultivation Base of Geographical Environment Evolution (Jiangsu Province) Nanjing 210023 China Department of Geography University of Wisconsin-Madison Madison 53706 USA

The automatic recognition of landforms is regarded as one of the most important procedures to classify landforms and deepen the understanding on the morphology of the earth. However, landform types are rather complex and gradual changes often occur in these landforms, thus increasing the difficulty in automatically recognizing and classifying landforms. In this study, small-scale watersheds, which are regarded as natural geomorphological elements, were extracted and selected as basic analysis and recognition units based on the data of SRTM DEM. In addition, datasets integrated with terrain derivatives(e.g., average slope gradient, and elevation range) and texture derivatives(e.g., slope gradient contrast and elevation variance) were constructed to quantify the topographical characteristics of watersheds. Finally, Random Forest(RF) method was employed to automatically select features and classify landforms based on their topographical characteristics. The proposed method was applied and validated in seven case areas in the Northern Shaanxi Loess Plateau for its complex andgradual changed landforms. Experimental results show that the highest recognition accuracy based on the selected derivations is 92.06%. During the recognition procedure, the contributions of terrain derivations were higher than that of texture derivations within selected derivative datasets. Loess terrace and loess mid-mountain obtained the highest accuracy among the seven typical loess landforms. However, the recognition precision of loess hill, loess hill–ridge, and loess sloping ridge is relatively low. The experiment also shows that watershed-based strategy could achieve better results than object-based strategy, and the method of RF could effectively extract and recognize the feature of landforms.

关键词： Landform recognition Random Forest Feature fusion DEM Loess landform

Translocation time of a polymer chain through an energy gradient nanopore

在线全文

学校读者我要写书评

暂无评论

Frontiers of physics 2017年第3期12卷 157-165页

作者： Meng-Bo Luo Shuang Zhang fan wu Li-Zhen Sun Department of Physics Zhejiang University Hangzhou 310027 China College of Science Qinzhou University Qinzhou 535011 China Department of Applied Physics Zhejiang University of Technology Hangzhou 310023 China

The translocation time of a polymer chain through an interaction energy gradient nanopore was studied by Monte Carlo simulations and the Fokker-Planck equation with double-absorbing boundary condi- tions. Both the simulation and calculation revealed three different behaviors for polymer translocation. These behaviors can be explained qualitatively from free-energy landscapes obtained for polymer translocation at different parameters. Results show that the translocation time of a polymer chain through a nanopore can be tuned by suitably designing the interaction energy gradient.

关键词： polymer chain translocation time nanopore Monte Carlo simulation Fokker-Planck equation

Modelling of a post-combustion carbon dioxide capture absorber using potassium carbonate solvent in Aspen Custom Modeller

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Chemical Engineering 2018年第11期26卷 2327-2336页

作者： Yue wu fan wu Guoping Hu Nouman R.Mirza Geoffrey W.Stevens Kathryn A.Mumford Department of Chemical and Biomolecular Engineering The University of Melbourne Peter Cook Centre for CCS Research The University of Melbourne Particulate Fluids Processing Centre (PFPC) The University of Melbourne

The process models for an equilibrium CO_2 absorber and a rate based CO_2 absorber using potassium carbonate(K2 CO3) solvents were developed in Aspen Custom Modeller(ACM) to remove CO_2 from a flue gas. The process model utilised the Electrolyte Non-Random Two Liquid(ENRTL) thermodynamic model and various packing correlations. The results from the ACM equilibrium model shows good agreement with an inbuilt Aspen Plus?model when using the same input conditions. By further introducing a Murphree efficiency which is related to mass transfer and packing hydraulics, the equilibrium model can validate the experimental results from a pilot plant within a deviation of 10%. A more rigorous rate based model included mass and energy flux across the interface and the enhancement effect resulting from chemical reactions. The rate based model was validated using experimental data from pilot plants and was shown to predict the results to within 10%. A parametric sensitivity analysis showed that inlet flue gas flowrate and K2 CO3 concentration in the lean solvent has significant impact on CO_2 recovery. Although both models can provide reasonable predictions based on pilot plant results, the rate based model is more advanced as it can explain mass and heat transfer, transport phenomena and chemical reactions occurring inside the absorber without introducing an empirical Murphree efficiency.

关键词： Equilibrium Rate based Modelling Absorber Potassium carbonate

Development of small-molecule viral inhibitors targeting various stages of the life cycle of emerging and re-emerging viruses

在线全文

学校读者我要写书评

暂无评论

Frontiers of Medicine 2017年第4期11卷 449-461页

作者： Xiaohuan Wang Peng Zou fan wu Lu Lu Shibo Jiang Shanghai Public Health Clinical Center & Key Laboratory of Medical Molecular Hrology of MOE/MOH of School of Basic Medical Sciences Fudan University Shanghai 200032 China Lindsley F. Kimball Research Institute New York Blood Center New York NY 10065 USA

In recent years, unexpected outbreaks of infectious diseases caused by emerging and re-emerging viruses have become more frequent, which is possibly due to environmental changes. These outbreaks result in the loss of life and economic hardship. Vaccines and therapeutics should be developed for the prevention and treatment of infectious diseases. In this review, we summarize and discuss the latest progress in the development of small-molecule viral inhibitors against highly pathogenic coronaviruses, including severe acute respiratory syndrome coronavirus and Middle East respiratory syndrome coronavirus, Ebola virus, and Zika virus. These viruses can interfere with the specific steps of viral life cycle by blocking the binding between virus and host cells, disrupting viral endocytosis, disturbing membrane fusion, and interrupting viral RNA replication and translation, thereby demonstrating potent therapeutic effect against various emerging and re-emerging viruses. We also discuss some general strategies for developing small-molecule viral inhibitors.

关键词： emerging and re-emerging viruses small-molecule inhibitor coronavirus Ebola virus Zika virus life cycle

Boosting near-infrared photothermal/photoacoustic conversion performance of anthracene-fused porphyrin via paramagnetic ion coordination strategy

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Science China Chemistry 2023年第1期66卷 164-173页

作者： fan wu Yufen Sun Hu Gao Xu Zhi Yue Zhao Zhen Shen State Key Laboratory of Coordination Chemistry Collaborative Innovation Center of Advanced MicrostructureSchool of Chemistry and Chemical EngineeringNanjing UniversityNanjing 210023China

Designing stable molecules with efficient near-infrared(NIR) photothermal/photoacoustic performances is important for biological and environmental *** this article,we inserted paramagnetic Cu(Ⅱ) into tetraanthraporphyrin to generate a highly photostable molecule,Cu ANP,which demonstrated a sharp,intense Q band in the NIR region above 800 *** its diamagnetic Zn(Ⅱ)-coordinated analog Zn ANP that degraded rapidly under ambient light,Cu ANP has a low-lying singly unoccupied d orbital that quenched radioactive emission and reactive oxygen generation effectively,resulting in fast photothermal response,excellent photothermal conversion capacity and heat endurance under long-term laser irradiation in an organic solvent,in water after encapsulation as a nanocomposite,and in the solid *** ANP also exhibited an intense NIR photoacoustic response and was reversibly redox-active,displaying a broad band spreading to the NIR-Ⅱ window(700–1,300 nm) in the dicationic *** paramagnetic metal incorporation strategy can enhance the performance of photolabile NIR absorbing molecules for a variety of photothermal/photoacoustic applications.

关键词： porphyrinoids photothermal photoacoustic near-infrared paramagnetic

Mapping winter rapeseed in South China using Sentinel-2 data based on a novel separability index

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2023年第6期22卷 1645-1657页

作者： TAO Jian-bin ZHANG Xin-yue wu Qi-fan WANG Yun Key Laboratory for Geographical Process Analysis&Simulation of Hubei Province/School of Urban and Environmental Sciences Central China Normal UniversityWuhan 430079P.R.China

Large-scale crop mapping using remote sensing data is of great significance for agricultural production, food security and the sustainable development of human societies. Winter rapeseed is an important oil crop in China that is mainly distributed in the Yangtze River Valley. Traditional winter rapeseed mapping practices are insufficient since they only use the spectral characteristics during the critical phenological period of winter rapeseed, which are usually limited to a small region and cannot meet the needs of large-scale applications. In this study, a novel phenology-based winter rapeseed index(PWRI) was proposed to map winter rapeseed in the Yangtze River Valley. PWRI expands the date window for distinguishing winter rapeseed and winter wheat, and it has good separability throughout the flowering period of winter rapeseed. PWRI also improves the separability of winter rapeseed and winter wheat, which traditionally have been two easily confused winter crops. A PWRI-based method was applied to the Middle Reaches of the Yangtze River Valley to map winter rapeseed on the Google Earth Engine platform. Time series composited Sentinel-2 data were used to map winter rapeseed with 10 m resolution. The mapping achieved a good result with overall accuracy and kappa coefficients exceeding 92% and 0.85, respectively. The PWRI-based method provides a new solution for high spatial resolution winter rapeseed mapping at a large scale.

关键词： phenology-based winter rapeseed index winter rapeseed mapping Sentinel-2 Google Earth Engine