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Journal of Molecular Cell Biology 2020年第8期12卷 644-653页

作者： Tijmen H.Booij wouter n.leonhard Hester Bange Kuan Yan Michiel Fokkelman Anna J.Plugge Kimberley A.M.Veraar Johannes G.Dauwerse Gerard J.P.van Westen Bob van de Water Leo S.Price Dorien j.M.Peters Division of Toxicology Leiden Academic Centre for Drug Research(LACDR)Leiden UniversityLeidenThe Netherlands Present address:nEXUS Personalized Health Technologies ETH ZurichSwitzerland Department of Human Genetics Leiden University Medical Center(LUMC)LeidenThe Netherlands OcellO B.V. LeidenThe Netherlands Division of Medicinal Chemistry Leiden Academic Centre for Drug Research(LACDR)LeidenThe Netherlands

Polycystic kidney disease(PKD)is a prevalent genetic disorder,characterized by the formation of kidney cysts that progressively lead to kidney *** currently available drug tolvaptan is not well tolerated by all patients and there remains a strong need for alternative *** signalling rewiring in PKD that drives cyst formation is highly complex and not fully *** a consequence,the effects of drugs are sometimes difficult to *** previously established a high-throughput microscopy phenotypic screening method for quantitative assessment of renal cyst ***,we applied this 3D cyst growth phenotypic assay and screened 2320 small drug-like molecules,including approved *** identified 81 active molecules that inhibit cyst ***-parametric phenotypic profiling of the effects on 3D cultured cysts discriminated molecules that showed preferred pharmacological effects above genuine toxicological ***,a triterpenoid from Tripterygium wilfordii,was identified as a potent inhibitor of cyst growth in *** an in vivo iKspCre-Pkd1^lox,lox mouse model for PKD,celastrol inhibited the growth of renal cysts and maintained kidney function.

关键词： PKD celastrol pyrvinium pamoate high-content screening 3D

First 150 years of catatonia:Looking back at its complicated history and forward to the road ahead

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World Journal of Psychiatry 2024年第5期14卷 600-606页

作者： Levente Csihi Gabor S Ungvari Stanley n Caroff Gábor Gazdag Department of Psychiatry and Psychiatric Rehabilitation Jahn Ferenc South Pest HospitalBudapest 1204Hungary Division of Psychiatry School of MedicineUniversity of Western AustraliaCrawley 6009Western AustraliaAustralia Section of Psychiatry University of Notre DameFremantle 6160Western AustraliaAustralia Behavioral Health Service Corporal Michael J CresenczVeterans Affairs Medical CenterPhiladelphiaPA 19104United States Department of Psychiatry University of PennsylvaniaPerelman School of MedicinePhiladelphiaPA 19104United States Department of Psychiatry and Psychotherapy Faculty of MedicineSemmelweis UniversityBudapest 1083Hungary

Karl Ludwig Kahlbaum(1828-1899)was the first to conceptualize and describe the main clinical features of a novel psychiatric illness,which he termed catatonia in his groundbreaking monograph published 150 years *** Kahlbaum postulated catatonia as a separate disease entity characterized by psychomotor symptoms and a cyclical course,a close examination of his 26 cases reveals that most of them presented with motor symptom complexes or syndromes associated with various psychiatric and medical *** his classification system,Kraepelin categorized catatonic motor symptoms that occur in combination with psychotic symptoms and typically have a poor prognosis within his dementia praecox(schizophrenia)disease *** of the substantial influence of Kraepelin’s classification,catatonia was predominantly perceived as a component of schizophrenia for most of the 20th ***,with the advent of the psychopharmacotherapy era starting from the early 1950s,interest in catatonia in both clinical practice and research subsided until the early *** past two decades have witnessed a resurgence of interest in *** Diagnostic and Statistical Manual of Mental Disorders Fifth Edition,marked a paradigmatic shift by acknowledging that catatonia can occur secondary to various psychiatric and medical *** introduction of an independent diagnostic category termed“Catatonia not Otherwise Specified”significantly stimulated research in this *** authors briefly review the history and findings of recent catatonia research and highlight promising directions for future exploration.

关键词： Catatonia Historical overview Psychopharmacology Diagnostic and Statistical Manual of Mental Disorders-5 Wernicke-Kleist-leonhard school

Efficient DnA-assisted synthesis of trans-membrane gold nanowires

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Microsystems & nanoengineering 2018年第1期4卷 490-497页

作者： Maoxiang Guo Iván Hernández-neuta narayanan Madaboosi Mats nilsson wouter van der Wijngaart Department of Micro and nanosystems KTH Royal Institute of TechnologyOsquldas väg 10Stockholm 10044Sweden Science for Life Laboratory Department of Biochemistry and BiophysicsStockholm UniversityTomtebodavägen 23 ASolna SE-17165Sweden

Whereas electric circuits and surface-based(bio)chemical sensors are mostly constructed in-plane due to ease of manufacturing,3D microscale and nanoscale structures allow denser integration of electronic components and improved mass transport of the analyte to(bio)chemical sensor *** work reports the first out-of-plane metallic nanowire formation based on stretching of DnA through a porous *** use rolling circle amplification(RCA)to generate long single-stranded DnA concatemers with one end anchored to the *** DnA strands are stretched through the pores in the membrane during liquid removal by forced *** the liquid–air interface movement across the membrane occurs in every pore,DnA stretching across the membrane is highly *** stretched DnA molecules are transformed into trans-membrane gold nanowires through gold nanoparticle hybridization and gold enhancement chemistry.A 50 fM oligonucleotide concentration,a value two orders of magnitude lower than previously reported for flat surface-based nanowire formation,was sufficient for nanowire *** observed nanowires in up to 2.7% of the membrane pores,leading to an across-membrane electrical conductivity reduction from open circuit to nal-to-noise ratio and can also be extended for use as a biosensor due to the high specificity and scope for multiplexing offered by RCA.

关键词： DnA stretching DnA detection gold nanowires nanowire synthesis membrane

numerical accuracy and convergence with EMC3-EIREnE

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Plasma Science and Technology 2020年第5期22卷 5-15页

作者： Kristel GHOOS Heinke FRERICHS wouter DEKEYSER Giovanni SAMAEY Martine BAELMAnS KU Leuven Department of Mechanical EngineeringB-3001LeuvenBelgium University of Wisconsin Madison Department of Engineering PhysicsWI 53706United States of America KU Leuven Department of Computer ScienceB-3001LeuvenBelgium

The iterative Monte Carlo(MC)code EMC3-EIREnE is frequently used for plasma edge simulations in 3 D *** far,a quantitative evaluation of the numerical quality of the code results remains an open *** this paper,we demonstrate a framework for the practical assessment of accuracy and convergence with ***,we provide a first accuracy analysis with EMC3-EIREnE for a DIII-D divertor edge plasma ***,we introduce post-processing averaging to efficiently reduce the variance of the statistical ***,we estimate the deterministic error contributions based on their theoretical reduction rates by comparing solutions with a different grid resolution,time step,or number of MC particles per ***,using parameterized expressions for the error and the computational time,suitable numerical parameters are determined to achieve faster and/or more accurate *** found that simulations can be more than twice as fast without losing accuracy by making use of post-processing averaging and choosing optimal *** addition,we conclude that the discretization error is the dominant error contribution for the case selected in this paper,which demonstrates the importance of constructing an adequate mesh.

关键词： convergence Monte Carlo numerics plasma edge EMC3-EIREnE

This work was supported by the European Research Council Advanced Grant(ETAP-742985 to *** J.F.)and by the Ministry of Science and Technology(grant 110-2636-B-005-001 to K.-J.L.).

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Plant Communications 2024年第1期5卷 130-147页

作者： Yu Zhang Yuan Fu Wenfei Xian Xiuli Li Yong Feng Fengjiao Bu Yan Shi Shiyu Chen Robin van Velzen Kai Battenberg Alison M.Berry Marco G.Salgado Hui Liu Tingshuang Yi Pascale Fournier nicole Alloisio Petar Pujic Hasna Boubakri M.Eric Schranz Pierre-Marc Delaux Gane Ka-Shu Wong Valerie Hocher Sergio Svistoonoff Hassen Gherbi Ertao Wang wouter Kohlen Luis G.Wall Martin Parniske Katharina Pawlowski Philippe normand Jeffrey J.Doyle Shifeng Cheng Shenzhen Branch Guangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of AgricultureAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 518124China Biosystematics Group Department of Plant SciencesWageningen University6708PB Wageningenthe Netherlands Department of Plant Sciences University of CaliforniaDavisDavisCA 95616USA Department of Ecology Environment and Plant SciencesStockholm University10691 StockholmSweden Germplasm Bank of Wild Species Kunming Institute of BotanyChinese Academy of SciencesLanhei RoadKunming 650201China Universite´de Lyon Universite´Lyon 1CNRSUMR5557Ecologie MicrobienneINRAUMR 141843 bd du 11 novembre 191869622 VilleurbanneFrance Laboratoire de Recherche en Sciences Ve´ge´tales(LRSV) Universite´de ToulouseCNRSUPS24 chemin de Borde RougeAuzevilleBP4261731326 Castanet TolosanFrance Department of Biological Sciences University of AlbertaEdmontonAB T6G 2E9Canada French national Research Institute for Sustainable Development(IRD) UMR LSTM(IRD/CIRAD/INRAe/Montpellier University/Supagro)-Campus International BaillarguetTA A-82/J34398 Montpellier Cedex 5France national Key Laboratory of Plant Molecular Genetics CAS Center for Excellence in Molecular Plant SciencesInstitute of Plant Physiology and EcologySIBSChinese Academy of SciencesShanghaiChina Laboratory of Molecular Biology Department of Plant SciencesWageningen University6708PB Wageningenthe Netherlands Laboratory of Biochemistry Microbiology and Soil Biological InteractionsDepartment of Science and TechnologyNational University of QuilmesCONICETBernalArgentina Faculty of Biology GeneticsLMU MunichGroßhaderner Strasse 2-482152 MartinsriedGermany School of Integrative Plant Science Sections of Plant Biology and Plant Breeding&GeneticsCornell UniversityIthacaNY 14853USA State Key Laboratory of Systematic and Evolutionary Botany Institute of BotanyChinese Academy of SciencesBeijing 100093China Univer

Plant root-nodule symbiosis(RnS)with mutualistic nitrogen-fixing bacteria is restricted to a single clade of angiosperms,the nitrogen-Fixing nodulation Clade(nFnC),and is best understood in the legume *** species share many commonalities,explained either by divergence from a common ancestor over 100 million years ago or by convergence following independent origins over that same time ***,comparative analyses of diverse nodulation syndromes can provide insights into constraints on nodulation—what must be acquired or cannot be lost for a functional symbiosis—and the latitude for Plant Communications Genomic landscape of nodulation variation in the ***,much remains to be learned about nodulation,especially outside of ***,we employed a large-scale phylogenomic analysis across 88 species,complemented by 151 RnA-seq libraries,to elucidate the evolution of *** phylogenomic analyses further emphasize the uniqueness of the transcription factor nIn as a master regulator of nodulation and identify key muta-tions that affect its function across the *** transcriptomic assessment revealed nodule-specific upregulated genes across diverse nodulating plants,while also identifying nodule-specific and nitrogen-response *** 70%of symbiosis-related genes are highly conserved in the four representative species,whereas defense-related and host-range restriction genes tend to be lineage *** study also identified over 900000 conserved non-coding elements(CnEs),over 300000 of which are unique to sampled nFnC ***-specific CnEs are enriched with the active H3K9ac mark and are correlated with accessible chromatin regions,thus representing a pool of candidate regula-tory elements for genes involved in ***,our results provide novel insights into the evolution of nodulation and lay a foundation for engineering of RnS traits in agriculturally important crops.

关键词： nitrogen-fixing root-nodule symbiosis two competing hypotheses phylogenomics phylotranscrip-tomics conserved non-coding elements convergence deep homology

康复卫生政策和系统研究对加强卫生系统中康复服务的重要性:呼吁采取行动并加速开展

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中国康复 2024年第4期39卷 251-253页

作者： Walter R.Frontera wouter DeGroote Abdul Ghaffar 康复卫生政策和系统研究小组 WHO康复培训与研究合作中心(武汉) 不详

具体机构及作者名单如下:美国物理医学与康复杂志(WRF);世界卫生组织康复计划(WDG);世界卫生组织卫生政策和系统研究联盟(WAG);康复卫生政策和系统研究小组(合作期刊主编按字母顺序排列):Iben Axen,DC,博士(脊椎按摩疗法和手法治疗)、Mu... 详细信息

具体机构及作者名单如下:美国物理医学与康复杂志(WRF);世界卫生组织康复计划(WDG);世界卫生组织卫生政策和系统研究联盟(WAG);康复卫生政策和系统研究小组(合作期刊主编按字母顺序排列):Iben Axen,DC,博士(脊椎按摩疗法和手法治疗)、Muhammad Ehab Azim,DPT,MS-nMPT(基础大学康复科学杂志)、Linamara Battistella,医学博士、哲学博士(Acta Fisiatrica)、克里斯蒂安·博格,医学博士、哲学博士(康复医学杂志)、Ines Campos,医学博士、理学硕士(葡萄牙物理与康复医学杂志)、罗德里戈·卡斯特罗,医学博士(Revista Columbiana de Medicina Físicay Rehabilitación),Joaquim Chaler,MD,Ph.D(Rehabilitación),Leighton Chan,MD,MPH(物理医学与康复档案室)。

关键词：卫生政策物理医学卫生系统康复计划哲学博士按摩疗法卡斯特罗作者名单

Sainfoin(Onobrychis viciifolia)silage in dairy cow rations reduces ruminal biohydrogenation and increases transfer efficiencies of unsaturated fatty acids from feed to milk

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Animal nutrition 2020年第3期6卷 333-341页

作者： nguyen Thi Huyen Martin W.A.Verstegen wouter H.Hendriks Wilbert F.Pellikaan Animal nutrition Group Wageningen UniversityPO Box 338Wageningen6700 AHthe Netherlands Department of Farm Animal Health Utrecht UniversityPO Box 80.163Utrecht3508 TDthe Netherlands

The effects of replacing grass silage by sainfoin silage in a total mixed ration(TMR)based diet on fatty acid(FA)reticular inflow and milk FA profile of dairy cows was *** experiment followed a crossover design with 2 dietary *** control diet consisted of grass silage,corn silage,concentrate and *** the sainfoin diet,half of the grass silage was replaced by a sainfoin *** rumen cannulated lactating multiparous dairy cows with a metabolic body weight of 132.5±3.6 kg BW^0.75,214±72 d in milk and an average milk production of 23.1±2.8 kg/d were used in the *** were paired based on parity and milk *** pairs,cows were randomly assigned to either the control diet or the sainfoin diet for 2 experimental periods(29 d per period).In each period,the first 21 d,cows were housed individually in tie-stalls for adaptation,then next 4 d cows were housed individually in climate-controlled respiration chambers to measure *** the last 4 d,cows were housed individually in tie stalls to measure milk FA profile and determine FA reticular inflow using the reticular sampling technique with Cr-ethylenediaminetetraacetic acid disodium salt dihydrate(EDTA)and Yb-acetate used as digesta flow *** the dietary C18:3n-3 intake was lower(P=0.025)in the sainfoin diet group,the mono-unsaturated FA reticular inflow was greater(P=0.042)in cows fed the sainfoin *** reticular inflow of trans-9,trans-12-C18:2 and cis-12,trans-10 C18:2 was greater(P≤0.024)in the sainfoin diet *** cows fed sainfoin diet had a lower(P≤0.038)apparent ruminal biohydrogenation of cis-9-C18:1 and C18:3n-3,compared to the cows fed the control *** sainfoin diet group had greater(P≤0.018)C18:3n-3 and cis-9,cis-12-C18:2 proportions in the milk FA profile compared to the control diet *** efficiencies from feed to milk of C18:2,C18:3n-3 and unsaturated FA were greater(P≤0.0179)for the sainfoin *** on the results,it co

关键词： Sainfoin silage Reticular inflow Milk fatty acid profile Dairy cow

Limited influences of chemotherapy on healthy and metastatic liver parenchyma

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World Journal of Gastroenterology 2005年第34期11卷 5322-5326页

作者： nancy Van Damme Pieter Demetter wouter De Bock Marianne Rottiers Marleen Praet Bernard de Hemptinne Marc Peeters Department of Gastroenterology Ghent University Hospital De Pintelaan 185 1K12IE Ghent 9000 Belgium Department of Pathology Ghent University Hospital De Pintelaan 185 Blok A Ghent 9000 Belgium Department of Surgery Ghent University Hospital De Pintelaan 185 2K12IC Ghent 9000 Belgium

AIM: To examine the expression of E-cadherin, β-catenin,γ-catenin, VEGF, and p53 in 39 patients with hepatic metastasis from colorectal cancer ***: The patients were divided into two groups:those (n = 16) who had no chemotherapy for at least 6 mo before the liver resections and those (n = 23)who were treated with chemotherapy before liver resections. A score from 0 to 3 was given for the number of positive cells and from 0 to 3 for the intensity of staining in these cells, in both healthy and metastatic liver ***: no significant differences in the expression of E-cadherin, β- and γ-catenin, VEGF and p53, could be observed between patients who received and did not receive chemotherapy, in both normal and metastatic liver ***: Despite the assumption that chemotherapy had an effect on liver metastasis, no influences were noticed immunohistochemically.

关键词： Chemotherapy Metastatic liver parenchyma Cadherin Catenin VEGF

Correction to:Efficient DnA-assisted synthesis of trans-membrane gold nanowires

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Microsystems & nanoengineering 2018年第1期4卷 298-299页

Figure 2 and the descriptive text in the Section“SEM characterization of synthesized nanowires”of the previously published version of this Article were *** authors would like to replace Fig.2 and section“SEM charac... 详细信息

关键词： nanowires Figure replace

CETP as a Target in HDL-raising Therapy:lessons from APOE*3-Leiden. CETP Transgenic Mice

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中国动脉硬化杂志 2009年第7期17卷 574-575页

作者： Willeke de Haan Jitske de Vries-van der Weij Caroline C. van der Hoogt José W.A. van der Hoorn Johannes A. Romijn J. wouter Jukema Louis M. Havekes Hans M.G. Princen Patrick C.n. Rensen Departments of General Internal Medicine Endocrinologyand Metabolic Diseases Departments of Human Genetics TnO-Biosciences Gaubius Laboratory Departments of Cardiology Leiden University Medical Center

Background Cardiovascular disease (CVD) is mainly caused by atherosclerosis,for which dyslipidemia (i.e. high (V)LDL-cholesterol (C),high triglycerides and low HDLC is a major risk factor. To reduce the risk to develo... 详细信息

关键词：心血管疾病动脉粥样硬化血脂胆固醇临床分析