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Trans-10,cis-12, not cis-9,trans-11, conjugated linoleic acid decreases ErbB3 expression in HT-29 human colon cancer cells

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World Journal of Gastroenterology 2005年第33期11卷 5142-5150页

作者： Han Jin Cho woo Kyoung Kim Jae In Jung Eun Ji Kim Soon Sung Lim dae Young Kwon Jung Han Yoon Park Department of Food Science and Nutrition Hallym University Chuncheon 200-702 Korea Department of Food Science and Nutrition Dankook University Seoul 140-714 Korea Department of Food Science and Nutrition Hallym University Chuncheon 200-702 Korea Silver Biotechnology Research Center Hallym University Chuncheon 200-702 Korea Korea Food Research Institute Songnam 463-746 Korea

AIM： To examine whether trans-10,cis-12 CLA （t10C12） or cis-9,trans-11 CLA （c9t11） inhibits heregulin （HRG）-β- stimulated cell growth and HRG-β-ErbB3 signaling in HT-29 cells. METHODS： We cultured HT-29 cells in the absence or presence of the CLA isomers and/or the ErbB3 ligand HRG-β. MTT assay, [^3H]thymidine incorporation, Annexin V staining, RT-PCR, Western blotting, immunoprecipitation, and in vitro kinase assay were performed. RESULTS： HRG-β increased cell growth, but did not prevent DNA t10c12-induced growth inhibition. T10C12 inhibited DNA synthesis and induced apoptosis of HT-29 cells, whereas c9t11 had no effect. T10c12 decreased the levels of ErbB1, ErbB2, and ErbB3 proteins and transcripts in a dose-dependent manner, whereas c9t11 had no effect. Immunoprecipitation/ Western blot studies revealed that t10c12 inhibited HRG- β-stimulated phosphorylation of ErbB3, recruitment of the p85 subunit of phosphoinositide 3-kinase （PI3K） to ErbB3, ErbB3-associated PI3K activities, and phosphorylation of Akt. However, c9t11 had no effect on phospho Aid： levels. Neither t10c12 nor c9t11 had any effect on HRG-β-induced phosphorylation of ERK-1/2. CONCLUSION： These results indicate that the inhibition of HT-29 cell growth by t10c12 may be induced via its modulation of ErbB3 signaling leading to inhibition of Akt activation.

关键词： Akt Phosphoinositide 3-kinase DNA synthesis Apoptosis ERK-1/2

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Potentiating activity of luteolin on membrane permeabilizing agent and ATPase inhibitor against methicillin-resistant Staphylococcus aureus

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Asian Pacific Journal of Tropical Medicine 2016年第1期9卷 16-19页

作者： dae-Ki Joung Young-Seob Lee Sin-Hee Han Sang-Won Lee Seon-woo Cha Su-Hyun Mun Ryong Kong Ok-Hwa kang Ho-Jun Song Dong-Won Shin Dong-Yeul Kwon Department of Oriental Pharmacy College of Pharmacy and Wonkwang-Oriental Medicines Research Institute.Wonkwang University Department of Herbal Crop Research National Institute of Horticultural & Herbal ScienceRDA BK21 Plus Team Professional Graduate School of Oriental MedicineWonkwang University Department of Oriental Medicine Resources Sunchon National University

Objective:To investigate the mechanism of antibacterial activity of luteoiin(LUT) against methicillin-resistant Staphylococcus aureus(MRSA).Methods:The mechanism of anti-MRSA activity of LUT was analyzed by the viability assay in membrane permeabilizing agent ATPase inhibitors,and peptidoglycan(PGN) derived from Staphylococcus aureus(***).Also,transmission electron microscopy was used to monitor survival characteristics and changes in *** ***:Compared to the LUT alone,the optical density of suspensions treated with the combination of 125 μg/mL Tris and 230 μg/mL DCCD were reduced to 60%and 46%,***(15.6 μg/mL) gradually impeded the activity of LUT,and PGN(62.5 μg/mL) completely blocked the activity of LUT on ***:Increased susceptibility to LUT with me Tris and DCCD combinations is evident in all tested MRSA *** results indicate LUT synergy in increasing cytoplasmic membrane permeability and inhibiting *** PGN directly blocks the antibacterial activity of LUT,suggesting the direct binding of LUT with *** findings may be validated for the development of antibacterial agent for low MRSA resistance.

关键词： Iuteolin Methicillin-resistant Staphylococcus aureus Membrane permeabilizing agent ATPase inhibitor Peptidoglycan

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Neuroprotective effects of roasted licorice,not raw form,on neuronal injury in gerbil hippocampus after transient forebrain ischemia

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Acta Pharmacologica Sinica 2006年第8期27卷 959-965页

作者： In-koo HWANG Soon-sung LIM Kyu-hyun CHOI Ki-yeon YOO Hyun-kyung SHIN Eun-ji KIM Jung-han YOONPARK Tae-cheon kang Young-sup KIM dae-young KWON Dong-woo KIM Won-kook MOON Moo-ho WON Korea Central Research Institute.Natural F&P Co Ltd Chunchon 200-160Korea Silver Biotechnology Research Center Korea Silver Biotechnology Research Center Department of Food Science and Nutrition Hallym UniversityChunchon 200-702Korea Medical Science Division Korea Research Institute of Chemical TechnologyYuseong.Daejeon 305-343Korea Korea Central Research Institute.Natural F&P Co Ltd Chunchon 200-160Korea Korea Silver Biotechnology Research Center

Aim:To observe neuroprotective effects of raw and roasted licorice against hy-poxia and ischemic ***:When elucidating the protective effects ofraw and roasted licorice,we analyzed the lactate dehydrogenase (LDH) releaseusing PC12 cells after hypoxia in an in vitro study and after transient forebrainischemia in an in vivo study on Mongolian ***:Raw and roastedlicorice significantly reduced LDH release from PC12 cells exposed to an hypoxicchamber for *** the roasted licorice-treated group,the decrease of LDH releasewas more pronounced compared to that of the raw licorice-treated *** licorice-treated animals,approximately 66%-71% of CA1 pyramidal cellsin the ischemic hippocampus were stained with cresyl violet compared to thecontrol ***,in the raw licorice-treated animals,no significantneuroprotection against ischemic damage was *** addition,ischemic ani-mals in roasted licorice-treated group maintained the Cu,Zn-superoxide dismutase(SOD1) activity and protein levels compared to the control group,while in rawlicorice-treated group SOD1 activity and protein levels were reduced *** pressure liquid chromatography analysis showed that non-polar compoundscontaining glycyrrhizin-degraded products,such as glycyrrhetinic acid (GA) andglycyrrhetinic acid monoglucuronide (GM),were increased in roasted ***:Roasted licorice had neuroprotective effects against ischemic dam-age by maintaining the SOD1 *** addition,the difference in protectiveability between raw and roasted licorice may be associated with non-polarcompounds,such as GA and GM.

关键词： transient ischemic attack roasted licorice raw licorice neurons PC12 cells hippocampus pyramidal cells Gerbillinae superoxide dismutase

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Flexible and transparent thin-film light-scattering photovoltaics about fabrication and optimization for bifacial operation

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npj Flexible Electronics 2023年第1期7卷 388-397页

作者： Soo-Won Choi Jae-Ho Park Ji-woo Seo Chaewon Mun Yonghun Kim Pungkeun Song Myunghun Shin Jung-dae Kwon Surface&Nano Materials Division Korea Institute of Materials ScienceChangwonGyeongnam 51508Republic of Korea Department of Materials Science and Engineering Pusan National UniversityBusan 46241Republic of Korea Cell R&D Team Hanhwa QCells202 Sansusandan 2-roLwol-myeonJincheon-gunChungcheongbuk-do 27816Republic of Korea School of Electronics and Information Engineering Korea Aerospace UniversityGoyangGyeonggi 10540Republic of Korea Department of Advance Materials Engineering University of Science and Technology(UST)217 Gajeong-roYuseong-guDaejeon 34113Republic of Korea

Flexible and transparent thin-film silicon solar cells were fabricated and optimized for building-integrated photovoltaics and bifacial operation.A laser lift-off method was developed to avoid thermal damage during the transfer of light-scattering structures onto colorless polyimide substrates and thus enhance front-incidence photocurrent,while a dual n-type rear window layer was introduced to reduce optical losses,facilitate electron transport for rear incidence,and thus enhance performance during bifacial *** introduction of the window layer increased the rear-to-front power conversion efficiency ratio to~86%.The optimized bifacial power conversion efficiency for front and rear irradiances of 1 and 0.3 sun,respectively,equaled 6.15%,and the average transmittance within 500–800 nm equaled 36.9%.Additionally,the flexible and transparent solar cells fabricated using laser lift-off exhibited good mechanical reliability(i.e.,sustained 500 cycles at a bending radius of 6 mm)and were therefore suitable for building-integrated photovoltaics.

关键词： transparent scattering film

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Electrically controlled mRNA delivery using a polypyrrolegraphene oxide hybrid film to promote osteogenic differentiation of human mesenchymal stem cells

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Nano Research 2022年第10期15卷 9253-9263页

作者： Huijung Kim Kübra Solak Yoojoong Han Yeon-woo Cho Kyeong-Mo Koo Chang-dae Kim Zhengtang Luo Hyungbin Son Hyung-Ryong Kim Ahmet Mavi Tae-Hyung Kim School of Integrative Engineering Chung-Ang University84 Heukseuk-roDongjak-guSeoul 06974Republic of Korea Department of Nanoscience and Nanoengineering Graduate School of Natural and Applied SciencesAtatürk UniversityErzurum 25240Turkey Department of Chemical and Biological Engineering Hong Kong University of Science and TechnologyKowloonHong Kong 999077China Department of Pharmacology College of DentistryJeonbuk National UniversityJeonju 54896Republic of Korea Department of Nanoscience and Nanoengineering Institute of ScienceAtatürk UniversityErzurum 25240Turkey Department of Mathematics and Science Education Education Faculty of Kazım KarabekirAtatürk UniversityErzurum 25240Turkey

Direct messenger ribonucleic acid(mRNA)delivery to target cells or tissues has revolutionized the field of ***,the applicability of regenerative medicine is limited by the technical difficulties of various mRNA-loaded ***,we report a new conductive hybrid film that could guide osteogenic differentiation of human adipose-derived mesenchymal stem cells(hADMSCs)via electrically controlled mRNA *** find optimal electrical conductivity and mRNAloading capacity,the polypyrrole-graphene oxide(PPy-GO)hybrid film was electropolymerized on indium tin oxide *** found that the fluorescein sodium salt,a molecule partially mimicking the physical and chemical properties of mRNAs,can be effectively absorbed and released by electrical stimulation(ES).The hADMSCs cultivated on the PPy-GO hybrid film loaded with pre-osteogenic mRNAs showed the highest osteogenic differentiation under electrical *** platform can load various types of RNAs thus highly promising as a new nucleic acid delivery tool for the development of stem cell-based therapeutics.

关键词： messenger ribonucleic acid(mRNA)delivery polypyrrole graphene oxide electrical release mesenchymal stem cells

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Severity of ultrasonographic liver steatosis and metabolic syndrome in Korean men and women

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World Journal of Gastroenterology 2005年第34期11卷 5314-5321页

作者： Hyeon Chang Kim Sung Hee Choi Hae Won Shin Jae Youn Cheong Kwan woo Lee Hyun Chul Lee Kap Bum Huh dae Jung Kim Department of Preventive Medicine Yonsei University College of Medicine Seoul South Korea Department of Internal Medicine Seoul National University College of Medicine Seongnam South Korea Department of Public Health Graduate School of Yonsei University Seoul South Korea Department of Gastroenterology Ajou University School of Medicine Suwon South Korea Department of Endocrinology and Metabolism Ajou University School of Medicine Suwon South Korea Department of Internal Medicine Yonsei University College of Medicine Seoul South Korea Huh's Diabetes Center and the 21~(st) Century Diabetes and Vascular Research Institute Seoul South Korea

AIM：To evaluate the association between the severity of liver steatosis and metabolic syndrome in apparently healthy Korean adults. METHODS： We examined 1022 men and women, aged 30-79 years, who participated in a health screening test. A standard interview, anthropometrics, biochemical studies, and abdominal ultrasonography were conducted for each participant. Metabolic syndrome was defined according to the National Cholesterol Education Program Adult Treatment Panel Ⅲ, with a modification for the waist circumference cut-off level. The severity of liver steatosis was evaluated using liver ultrasonography, and serum aspartate aminotransferase（AST）, alanine aminotransferase （ALT）, and γ-glutamyl transferase （γ-GT） levels were determined. RESULTS： Ultrasonographic liver steatosis was strongly associated with metabolic syndrome and common metabolic abnormalities. Compared with people without steatosis, people with mild, moderate, and severe steatosis had adjusted odds ratios for metabolic syndrome of 2.72 （95% CI, 1.01-2.94）, 2.89 （1.75-4.76） and 3.53 （1.25-9.98） in men, and 2.86 （1.64-5.01）, 3.19 （1.80-5.65） and 3.70 （0.82-16.73） in women, respectively. The serum AST level was not associated with metabolic syndrome. The serum ALT and γ-GT levels were significantly associated with metabolic syndrome in men but not in women.CONCLUSION： The occurrence of metabolic syndrome shows a stronger association with the severity of ultrasonographic steatosis than with the serum liver enzyme levels. The degree of fatty infiltration detected on ultrasonography can be used as an indicator of liver dysfunction attributable to metabolic abnormalities.

关键词： Metabolic syndrome Liver steatosis Nonalcoholic fatty liver disease Ultrasonography

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Dendritic cell-based immunotherapy for colon cancer using an HLA-A＊0201-restricted cytotoxic T-lymphocyte epitope from tumor-associated antigen 90K

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Cellular & Molecular Immunology 2013年第3期10卷 275-282页

作者： Ji Hee Lee Myong-Suk Park Jun-Eul Hwang Sang-Hee Cho woo-Kyun Bae Hyun-Jeong Shim dae-Eun Kim Ik-Joo Chung Department of Hematology-Oncology Chonnam National University Medical School Gwangju Korea The Brain Korea 21 Project Center for Biomedical Human Resources at Chonnam National University Gwangiu Korea

Tumor-associated antigen 90K is implicated in cell-cell and cell-extracellular matrix adhesion through its interaction with galectin-3 and integrin-β 1 and is highly expressed in malignant tissues, making it a novel target for the development of new immunotherapies. We investigated a potential immunotherapy treatment for colon cancer using 90K-specific cytotoxic T lymphocytes induced by autologous dendritic cells and pulsed with 90K peptides. We selected three peptides （90K3s1, 90K5 and 90K523） that bind to HLA-A＊0201 molecules on the basis of their binding affinity, as determined by a peptide-T2 binding assay. Dendritic cells pulsed with 90K peptides resulted in the efficient generation of mature dendritic cells and exhibited enhanced T-cell stimulation and polarization of naive T cells toward Thl. Dendritic cells pulsed with 90K peptides generated potent cytotoxic T-lymphocytes that lysed T2 cells loaded with each 90K peptide, and 90K＋/HLA-A2＋ colon cancer cell lines, including HCT116 and SW480, in a dose-dependent and HLA-A＊O2Ol-restricted manner. No killing was observed in 90K＋/HLA-A2- DLD1 or 90K-/HLA-A2- K562 cells. Therefore, we believe that cytotoxic T-lymphocytes stimulated by 90K peptide-pulsed dendritic cells naturally recognize the 90K peptide presented by colon cancer cells in the context of HLA-A2, and kill 90K-positive tumor cells. Dendritic cells pulsed with 90K peptides led to the induction of granzyme B and perforin positive CD8＋ T cells against HCT116 and SW480 cells, but not DLD1 cells. In conclusion, 90K-specific cytotoxic T lymphocytes, generated by stimulating T cells with 90K peptide-pulsed dendritic cells, could be useful effector cells for the immunotherapv treatment of colon cancer.

关键词： 9OK colon cancer dendritic cells immunotherapy peptide

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Healing of the nasal septal mucosa in an experimental rabbit model of mucosal injury

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World Journal of Otorhinolaryngology-Head and Neck Surgery 2017年第1期3卷 -页

作者： Kyu Young Choi Sung woo Cho Jun-Jae Choi Yu-Lian Zhang dae woo Kim Doo Hee Han Hyun Jik Kim Dong-Young Kim Chae-Seo Rhee Tae-Bin Won Department of Otorhinolaryngology-Head and Neck Surgery Hallym University College of MedicineKangnam Sacred Heart HospitalSeoul07441South Korea Department of Otorhinolaryngology-Head and Neck Surgery Seoul National University College of MedicineSeoul National University HospitalSeoul03080South Korea

Objective:The aim of this study was to investigate the regeneration process of the nasal mucosa after a surgically created mucosal defect in the rabbit nasal septum,and to evaluate the effects of different ***:A 7 mm-diameter circular mucosal defect was made in the septum of forty New Zealand white *** rabbits were divided into four groups (ten rabbits in each group) according to the type of intervention;no treatment (control),silastic sheet (SS),hyaluronic acid (HA),and silastic sheet and hyaluronic acid (SS + HA) *** diameter of the defect,mucosal thickness,epithelial thickness,and ciliated cell count were evaluated every week for five ***:The average diameter of the defect in the control group were 5.1,3.65,1.2,0.75,and 0.05 mm at postoperative 1,2,3,4,and 5 *** the SS group,the diameter decreased to 4.35,2.1,0.35,0.15,and 0 mm at postoperative 1,2,3,4,and 5 weeks,respectively,in which the mean diameter of the postoperative week 2 was significantly smaller compared to control (3.65 mm vs.2.1 mm,P =0.039).For the HA group and SS + HA group,the diameter of the defect did not show a significant difference from the control group during the five *** mucosal thickness,epithelial thickness,and ciliated cell count of the regenerated mucosa were not significantly different among the ***:The regeneration process of the nasal septal mucosa was identified using a novel rabbit *** regeneration can be accelerated by applying silastic sheets.

关键词： Nasal septum Nasal mucosa Mucous membrane Hyaluronic acid Rabbits

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Dual-priming oligonucleotide-based multiplex PCR using tissue samples in rapid urease test in the detection of Helicobacter pylori infection

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World Journal of Gastroenterology 2014年第21期20卷 6547-6553页

作者： woo Chul Chung Sung Hoon Jung Jung Hwan Oh Tae Ho Kim dae Young Cheung Byung wook Kim Sung Soo Kim Jin Il Kim Eun Young Sin Department of Internal Medicine the Catholic University of KoreaSeoul 130-709South Korea The Research Institute of St. Vincent Hospital the Catholic University of KoreaSuwon 442-723South Korea

AIM: To investigate whether tissue samples processed by the rapid urease test (RUT) kit are suitable for dual-priming oligonucleotide-based multiplex polymerase chain reaction (DPO-PCR) to detect Helicobacter pylori (... 详细信息

关键词： Helicobacter pylori Diagnosis Dual-priming oligonucleotide-based multiplex polymerase chain reaction

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Overexpression of Arabidopsis YUCCA6 in Potato Results in High-Auxin Developmental Phenotypes and Enhanced Resistance to Water Deficit

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Molecular Plant 2013年第2期6卷 337-349页

作者： Jeong Im Kim Dongwon Baek Hyeong Cheol Park Hyun Jin Chun Dong-Ha Oh Min Kyung Lee Joon-Yung Cha Woe-Yeon Kim Min Chul Kim woo Sik Chung Hans J. Bohner Sang Yeol Lee Ray A. Bressan Shin-woo Lee dae-Jin Yun Department of Biochemistry Purdue University West Lafayette IN 47907 USA Division of Applied Life Science (BK21 and WCU Program) Plant Molecular Biology and Biotechnology Research Center Gyeongsang National UniversityJinju 660-701 Republic of Korea Department of Plant Biology University of Illinois at Urbana-Champaign Urbana IL USA Department of Agronomy and Medicinal Plant Resources Gyeongnam National University of Science and Technology Jinju 660-758 Korea Faculty of Science King Abdulaziz University Jeddah 21589 KSA Department of Horticulture Purdue University West Lafayette IN 47907 USA

Indole-3-acetic acid （IAA）, a major plant auxin, is produced in both tryptophan-dependent and tryptophan-independent pathways. A major pathway in Arabidopsis thaliana generates IAA in two reactions from tryptophan. Step one converts tryptophan to indole-3-pyruvic acid （IPA） by tryptophan aminotransferases followed by a rate-limiting step converting IPA to IAA catalyzed by YUCCA proteins. We identified eight putative StYUC （Solanum tuberosum YUCCA） genes whose deduced amino acid sequences share 50%-70% identity with those of Arabidopsis YUCCA proteins. All include canonical, conserved YUCCA sequences： FATGY motif, FMO signature sequence, and FAD-binding and NADP-binding sequences. In addition, five genes were found with -50% amino acid sequence identity to Arabidopsis trypto-phan aminotransferases. Transgenic potato （Solanum tuberosum cv. Jowon） constitutively overexpressing Arabidopsis AtYUC6 displayed high-auxin phenotypes such as narrow downward-curled leaves, increased height, erect stature, and longevity. Transgenic potato plants overexpressing AtYUC6 showed enhanced drought tolerance based on reduced water loss. The phenotype was correlated with reduced levels of reactive oxygen species in leaves. The results suggest a func-tional YUCCA pathway of auxin biosynthesis in potato that may be exploited to alter plant responses to the environment.

关键词： Solanum tuberosum potato Arabidopsis thaliana yuc6-1D YUCCA, StYUCCA, StTAA, auxin, drought.

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