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A truncated hepatitis E virus ORF2 protein expressed in tobacco plastids is immunogenic in mice

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World Journal of Gastroenterology 2006年第2期12卷 306-312页

作者： Yuan-Xiang Zhou Maggie Yuk-Ting Lee James Ming-Him Ng Mee-Len Chye wing-kin yip Sze-Yong Zee Eric Lam Department of Botany The University of Hong Kong PokfulamRoad Hong Kong China Biotech Center Rutgers University 59 Dudley RoadNew Brunswick NJ 08901-8520 United States

AIM： To cost-effectively express the 23-ku pE2, the most promising subunit vaccine encoded by the E2 fragment comprising of the 3＇-portion of hepatitis E virus （HEV） open reading frame 2 （ORF2） in plastids of tobacco （Nicotiana tabacum cv. SR1）, to investigate the transgene expression and pE2 accumulation in plastids, and to evaluate the antigenic effect of the plastid-derived pE2 in mice. METHODS： Plastid-targeting vector pRB94-E2 containing the E2 fragment driven by rice psbA promoter was constructed. Upon delivery into tobacco plastids, this construct could initiate homologous recombination in psaB-trnfM and trnG-psbC fragments in plastid genome, and result in transgene inserted between the two fragments. The pRB94-E2 was delivered with a biolistic particle bombardment method, and the plastid-transformed plants were obtained following the regeneration of the bombarded leaf tissues on a spectinomycin-supplemented medium. Transplastomic status of the regenerated plants was confirmed by PCR and Southern blot analysis, transgene expression was investigated by Northern blot analysis, and accumulation of pE2 was measured by ELISA. Furthermore, protein extracts were used to immunize mice, and the presence of the pE2-reactive antibodies in serum samples of the immunized mice was studied by ELISA. RESULTS： Transplastomic lines confirmed by PCR and Southern blot analysis could actively transcribe the E2 mRNA. The pE2 polypeptide was accumulated to a level as high as 13.27 μg/g fresh leaves. The pE2 could stimulate the immunized mice to generate pE2-specific antibodies. CONCLUSION： HEV-E2 fragment can be inserted into the plastid genome and the recombinant pE2 antigen derived is antigenic in mice. Hence, plastids may be a novel source for cost-effective production of HEV vaccines.

关键词： Hepatitis E virus E2 Plastid transformation Vaccine Tobacco

Pathological mechanisms and therapeutic strategies for Alzheimer's disease

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Neural Regeneration Research 2022年第3期17卷 543-549页

作者： Yaojun Ju kin yip Tam Faculty of Health Sciences University of MacaoAvenida de UniversidadeTaipaMacao Special Adiministrative RegionChina

Alzheimer's disease is a rather complex neurodegenerative disease,which is attributed to a combination of multiple *** the many pathological pathways,synaptic dysfunctions,such as synapses loss and deficits in synaptic plasticity,were thought to be strongly associated with cognitive *** deficiencies in various sorts of neurotransmissions are responsible for the multifarious neurodegenerative symptoms in Alzheimer's disease,for example,the cholinergic and glutamatergic deficits for cognitive decline,the excitatory and inhibitory neurotransmission dyshomeostasis for synaptic plasticity deficits and epileptiform symptoms,and the monoamine neurotransmission for neuropsychiatric *** cascade hypothesis is the most popular pathological theory to explain Alzheimer's disease pathogenesis and attracts considerable *** lines of genetic and pathological evidence support the predominant role of amyloid beta in Alzheimer's disease *** tangles assembled by microtubule-associated protein tau are other important histopathological characteristics in Alzheimer's disease *** of tau toxicity was proved to lead to neuron damage,neuroinflammation and oxidative stress in *** is the main risk factor of neurodegenerative diseases,and is associated with inflammation,oxidative stress,reduced metabolism,endocrine insufficiencies and organ *** aging related risk factors were also proved to be some of the risk factors contributing to Alzheimer's *** Alzheimer's disease drug development,many good therapeutic strategies have been investigated in clinical ***,complex mechanism of Alzheimer's disease and the interplay among different pathological factors call for the come out of allpowerful therapies with multiple curing *** review seeks to summarize some of the representative treatments targeting different pathological pathways currently under clinical ***

关键词： Alzheimer's disease pathological pathways drug development multiple pathologies

Theoretical study of substituent effect on the charge mobility of 2,5-bis(trialkylsilylethynyl)-1,1,3,4-tetraphenylsiloles

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Science China Chemistry 2010年第11期53卷 2311-2317页

作者： LAM Jacky wing yip Department of Chemistry The Hong Kong University of Science & Technology

The theoretical calculation of the charge mobility of 2,5-bis(trialkylsilylethynyl)-1,1,3,4-tetraphenylsiloles is presented.B3LYP/6-31* calculations demonstrated that these silole molecules possessed large coupling matrix elements and reorganization energies for electron and hole transfers and high electron *** bulkiness of the trialkyl substituents influenced the charge mobility of the silole molecules,with the smaller trimethyl group imparting higher charge mobility than triethyl and triisopropyl substituents.

关键词： silole theoretical calculation substitution effect electronic structure charge mobility

A facile design for multifunctional AIEgen based on tetraaniline derivatives

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Science China Chemistry 2019年第6期62卷 732-738页

作者： Beibei Liu Wei He Hao Lu Kun Wang Mingming Huang Ryan Tsz kin Kwok Jacky wing yip Lam Longcheng Gao Jiping Yang Benzhong Tang Key laboratory of Aerospace Advanced Materials and Performance Ministry of Education School of Materials Science and EngineeringBeihang University Department of Chemistry HKUST Jockey Club Institute for Advanced Study Institute of Molecular Functional Materials Division of Biomedical Engineering State Key Laboratory of Molecular Neuroscience Division of Life Science The Hong Kong University of Science and Technology Laboratory of Bio-Inspired Smart Interfacial Science and Technology of Ministry of Education Key Laboratory of Beijing Energy School of Chemistry and Environment Beihang University

Aniline oligomers have been widely used in many fields due to their excellent physicochemical properties. Owing to strong intermolecular interactions, their emission is always weakened or quenched when they are in high concentration or aggregated state, which greatly limits their fluorescent applications. Inspired by the concept of aggregation-induced emission(AIE), herein we introduced large steric groups onto the aniline oligomer to prevent the formation of packing structure. In particular, diphenyl vinyl group was bonded with oligomeric tetraaniline by a facile synthetic procedure with high yield. The obtained aniline oligomer derivative exhibited typical AIE features, which was also confirmed by density functional theoretical calculation. More importantly, this AIE oligomer was able to detect Fe^(3+) ions selectively and quantitatively. The fluorescence intensity decreased linearly along with the increment of Fe^(3+) concentration. Moreover, we demonstrated that this AIE oligomer could stain live bacteria, such as E. coli and S. aureus efficiently. All these results suggest that such a readily accessible and multifunctional tetraaniline derivative provides a new platform for the construction of fluorescent materials.

关键词： tetraaniline aggregation-induced emission Fe3+ ion detection bacteria imaging

Crystalline Behavior and Structure of a Liquid Crystal Com- pound, 5-{[4＇-（（（Pentyl）oxy）-4-biphenylyl）carbonyl]oxy}- 1 -pentyne

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Chinese Journal of Chemistry 2011年第4期29卷 631-638页

作者：刘雷静李杲周恩乐 Lam,Jacky wing yip 唐本忠 State Key Laboratory of Supramolecular Structure and Materials Jilin University Changchun Jilin 130012 China State Key Laboratory of Polymer Physics and Chemistry Changchun Institute of Applied Chemistry Chinese Academy of Sciences Graduate School of Chinese Academy of Sciences Changchun Jilin 130022 China Department of Chemistry Hong Kong University of Science & Technology Clear Water Bay Kowloon Hong Kong China

Single crystals of two liquid crystal compounds, 5-{[4＇-（（（pentyl）oxy）-4-biphenylyl）carbonyl]oxy}-1-pentyne （A3EO5） and 5-{[（4＇-nonyloxy-4-biphenylyl）carbonyl]oxy}-1-pentyne （A3EO9）, have been prepared by solution growth technique. The morphologies and structures of A3EO5 and A3EO9 crystals were investigated by wide angle X-ray diffraction （WXRD）, atom force microscope （AFM） and transmission electron microscope （TEM）. In contrast to the same series of compounds which have a longer alkyl tail, 5-{[（4＇-heptoxy-4-biphenylyl）carbonyl]oxy}-1-pentyne （A3EO7）, 5-{[（4＇-heptoxy-4-biphenylyl）oxy]carbonyl}-1-pentyne （A3E＇O7） and A3EO9, A3EO5 shows strikingly different crystalline behavior. The former three compounds have only one crystal form, whereas A3EO5 exhibits polymorphism. Specifically, A3EO5 crystals grown from toluene solution show two crys- tal forms. The first one is crystal I which adopts a monoclinic P112/m space group with unit cell parameters of a= 5.79A, b=8.34A, c=43.92/k, y=96°, and the other one is crystal II which adopts a monoclinic P112 space group with unit cell parameters of a=5.55A, b=7.38A, c=31.75 ]k, y=94°. When using dioxane as the solvent to grow A3EO5 crystal, we can selectively obtain crystal Ⅰ. A3EO5 melt-grown crystals also have two crystal forms which derive from crystal I and crystal Ⅱ, respectively. The different crystalline behavior of the compounds should correlate with their different electron dipole moment resulting from the different length of alkyl tail.

关键词： crystal growth crystal morphology crystal structure electron diffraction single crystal

Photoelectrochemical degradation of Methylene Blue with β-PbO_2 electrodes driven by visible light irradiation

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Journal of Environmental Sciences 2011年第6期23卷 998-1003页

作者： Guoting Li HoYin yip kin Hang Wong Chun Hu Jiuhui Qu Po Keung Wong Department of Environmental and Municipal Engineering North China University of Water Conservancy and Electric Power Zhengzhou 450011 China School of Life Sciences The Chinese University of Hong Kong Shatin N.T. Hong Kong SAR China State Key Laboratory of Environmental Aquatic Chemistry Research Center for Eco-Environmental Sciences Chinese Academy of Sciences Beifing 100085 China

β-PbO2 electrodes were prepared by electro-deposition and characterized by scanning electron microscopy,X-ray diffraction,X-ray photoelectron spectroscopy,and linear sweep *** confirmed pure β-PbO2 crystals were on the electrode and it had a high oxygen evolution *** photoactivity and photoelectrochemical（PEC） properties of the β-PbO2 electrode were investigated under visible light irradiation（λ 〉 420 nm） for the decolorization of Methylene *** first-order kinetics parameter（Kapp） for dye decolorization using the β-PbO2 electrode achieved 6.71×10-4 min-1 under visible light irradiation,which indicated its excellent visible light-induced *** Kapp of the PEC process was as much as 1.41×10-3 min-1 and was 1.71 times that of visible light irradiation or electrolysis even in the presence of the β-PbO2 electrode.A significant synergetic effect was observed in the PEC *** also employed TiO2 modified β-PbO2 electrodes in this test,which revealed that the TiO2 immobilized on the β-PbO2 electrode inhibited the visible light-induced PEC efficiency despite the amount of TiO2 used for electrode *** β-PbO2 electrode was also superior to the dimensionally stable anode（Ti/Ru0.3Ti0.7O2） in visible light-induced photoactivity and PEC efficiency.

关键词： photocatalysis photoelectrochemical process visible light β-PbO2 electrode

Synthesis of an AIE-active fluorogen and its application in cell imaging

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Science China Chemistry 2009年第1期52卷 15-19页

作者： LAM Jacky wing yip FAISAL Mahtab NG Ka Ming LUO Kathy Qian Department of Chemistry The Hong Kong University of Science & Technology (HKUST) Clear Water Bay Department of Chemical and Biomolecular Engineering HKUST Clear Water Bay Bioengineering Graduate Program HKUST Clear Water Bay

A fluorogen named 1-decyl-1-methyl-2,5-bis{4-[(N,N-diethylamino)methyl]phenyl}-3,4-diphenylsilole (3) was synthesized. It emitted weakly as isolated molecule but strongly as supramolecular aggregate, showing a characteristic behavior of aggregation-induced emission (AIE). The molecules of 3 formed highly emissive nanoparticles in aqueous media, which quickly and selectively marked cytoplasm of HeLa cells and posed no toxicity to the living cells. The fluorogen is thus a promising candidate mate-rial for cell imaging as a sensitive, selective and cytocompatible biosensor.

关键词： aggregation-induced emission biosensor cell imaging

Organic Solar Cells at Stratospheric Condition for High Altitude Platform Station Application

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Chinese Journal of Chemistry 2022年第24期40卷 2927-2932页

作者： Ram Datt Harrison Ka Hin Lee Guichuan Zhang Hin-Lap yip wing Chung Tsoi SPECIFIC Faculty of Science and EngineeringSwansea UniversityBay CampusFabian WaySwanseaSA18EN United Kingdom Department of Physics City University of Hong KongKowloonHong KongSARChina School of Semiconductor Science and Technology South China Normal UniversityFoshanGuangdong528225 China State Key Laboratory of Luminescent Materials and Devices South China University of Technology381 Wushan RoadGuangzhouGuangdong510640 China Department of Materials Science and Engineering City University of Hong KongKowloonHong KongChina School of Energy and Environment City University of Hong KongKowloonHong KongChina Hong Kong Institute for Clean Energy City University of Hong KongKowloonHong KongChina

High specific power or power to mass ratio is a critical concern of photovoltaic(PV)for aerospace *** solar cells(OSCs)have advantages such as high absorption coefficient,compatibility with flexible substrate,light-weight,***,recently OSCs achieved power conversion efficiency(PCE)over 20%with the incorporation of the non-fullerene based small molecule acceptor and high specific power is believed to be *** enter the market,high-altitude platform station(HAPS)is perhaps the first place to start *** this work,we explore and compare the in-situ performance of two high performing OSCs,using the same donor but different acceptors,in mimic HAPS environment where the pressure,temperature and the illumination conditions are *** found that the use of acceptor can result in substantial difference in the performance at low temperatures.

关键词： Organic solar cells Stratosphere High-altitude platform station Low-temperature physics Donor-acceptor systems

HYPERBRANCHED CONJUGATIVE MACROMOLECULES CONSTRUCTED FROM TRIPLE-BOND BUILDING BLOCKS

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Chinese Journal of Polymer Science 2005年第6期23卷 567-591页

作者： Matthias HaeuBler Hong-chen Dong Jacky wing yip Lam Rong-hua Zheng An-jun Qin Ben-zhong Tang Department of Chemistry The Hong Kong University of Science ＆ Technology Clear Water Bay Kowloon Hong Kong China Department of Polymer Science and Engineering Zhejiang University Hangzhou 310027 China

Polycyclotrimerization and polycoupling of acetylenic monomers respectively furnish hyperbranched polyarylenes and polyynes with high molecular weights （up to 1 × 10^6） in high yields （up to 99.9%）. The polymers possess low intrinsic viscosities and high thermal stabilities, losing little of their weights when heated to 〉 400℃. Upon pyrolysis at 〉 800℃, the polymers graphitize with high char yields （up to 86%）. Hyperbranched polyarylenes efficiently emit deep-blue to blue-green lights with fluorescence quantum yields up to 98% and strongly attenuate intense laser pulses with optical power-limiting performances superior to that of C60, a well-known optical limiter. Poly（alkenephenylenes）, poly（aroylarylenes） and polyynes are readily cross-linkable by UV irradiation, serving as excellent photoresist materials for the generation of patterns with nanometer resolution. Thin films of hyperbranched polyynes exhibit very high refractive indexes （n up to 1.86）. The internal and terminal acetylene moieties of the polyynes readily form complexes with cobalt carbonyls, which can be transformed into soft ferromagnetic ceramics with high magnetic susceptibilities （Ms up to ca. 118 emu/g） and near-zero magnetic losses.

关键词： Hyperbranched polymers Alkyne polycyclotrimerization Alkyne polycoupling Polyarylenes Polyynes Optical materials Photoresists Soft ferromagnetic ceramics