检索结果-南通市图书馆

COX-2 polymorphisms-765G→C and-1195A→G and colorectal cancer risk

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2009年第36期15卷 4561-4565页

作者： Juliёt H Hoff Rene hm te Morsche Hennie MJ Roelofs Elise MJ van der Logt Fokko M Nagengast wilbert hm peters Department of Gastroenterology Radboud University Nijmegen Medical Center6500 HB NijmegenThe Netherlands

AIM： TO determine the possible modulating effect of the COX-2 polymorphisms, -765G→C and -1195A→G, on the risk of colorectal cancer （CRC） in a Dutch population. METHODS： This case-control study includes 326 patients with CRC and 369 age- and gender-matched controls. Genotypes of the COX-2 polymorphisms -7dEG→C and -1195A→G were determined by polymerase chain reaction-based restriction fragment length polymorphism. COX-2 genotypes and haplotypes were analyzed and odds ratios with 95% confidence intervals were estimated by logistic regression. RESULTS： The -765GG genotype was associated with an increased risk of developing CRC （OR, 1.45; 95% CI, 1.03-2.04）. No significant difference was observed in the genotype distribution of the -1195A→G polymorphism between patients and controls. The GG/AC haplotype was present significantly less often in patients than in controls （OR 0.44; 95% CI, 0.22-0.85）. When the AC, AG and GG haplotypes were investigated separately, the AC haplotype showed a tendency to be less frequent in patients than in controls （OR（AG/AC） 0.78; 95% CI, 0.57-1.06）. CONCLUSION： The -765GG genotype is associated with an increased risk of developing CRC and the G6/ AC haplotype seems to protect against CRC. These findings suggest a modulating role for the COX-2 polymorphisms -765G→C and -1195A→G in the development of CRC in a Dutch population.

关键词： Colorectal carcinoma Cyclooxygenase-2 Genetic polymorphism

Assessment of oxidative stress in chronic pancreatitis patients

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2006年第35期12卷 5705-5710页

作者： Mariette Verlaan Hennie MJ Roelofs Annie van Schaik Geert JA Wanten Jan BMJ Jansen wilbert hm peters Joost PH Drenth Department of Gastroenterology Radboud University Nijmegen Medical Centre The Netherlands

AIM: To assess the levels of antioxidant capacity and oxidative damage in blood of chronic pancreatitis (CP) patients in comparison with those in healthy control sub- jects, by using several different analytical techniques. METHODS: Thirty-five CP patients and 35 healthy con- trol subjects were investigated prospectively with re- spect to plasma levels of thiols, ferric reducing ability of plasma (FRAP, i.e. antioxidant capacity), levels of protein carbonyls and thiobarbituric acid reactive substances (TBARS). Additionally, we evaluated the production of reactive oxygen species (ROS) in whole blood. RESULTS: The antioxidative thiols including cysteine, cysteinylglycine and glutathione were significantly lower in CP patients. In addition, the non-enzymatic antioxi- dant capacity was significantly lower in CP patients, which correlated with the amount of oxidative protein (protein carbonyls) and the extent of lipid damage (TBARS), both were significantly higher in CP patients. The ROS production in whole blood after stimulation with phorbol 12-myritate 13-acetaat, demonstrated a strong tendency to produce more ROS in CP patients. CONCLUSION: Oxidative stress may contribute to the pathogenesis of chronic pancreatitis by decreasing anti- oxidant capacity and increasing oxidative damage in CP patients may be a rationale for intervention with antioxi- dant therapy.

关键词： Chronic pancreatitis Oxidative stress Thiols Ferric reducing ability of plasma Protein carbonyls Thiobarbituric acid reactive substances Reactive oxygen species

Cyclooxygenase-2 polymorphisms and the risk of esophageal adeno-or squamous cell carcinoma

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2009年第28期15卷 3493-3497页

作者： Jón O Kristinsson Paul van Westerveld Rene hm te Morsche Hennie MJ Roelofs T Wobbes Ben JM Witteman Adriaan CITL Tan Martijn GH van Oijen Jan BMJ Jansen wilbert hm peters Department of Gastroenterolog Radboud University Nijmegen Medical Center PO Box 9106500 HB Nijmegen The Netherlands Department of Surgery Radboud UniversityNijmegen Medical Center PO Box 9101 6500 HB NijmegenThe Netherlands Department of Gastroenterology Hospital Gelderse Vallei PO Box 9025 6710 HN Ede The Netherlands Department of Gastroenterology Canisius Wilhelmina Hospital Weg door Jonkerbos 100 6532 SZ Nijmegen The Netherlands

AIM： TO determine whether -1195 A→G and/or -765 G→C polymorphisms in Cyclooxygenase-2 CCOX-2） may have a risk modifying effect on the development of esophageal carcinoma in a Dutch Caucasian population. METHODS： Two study groups were recruited, 252 patients with esophageal carcinoma and 240 healthy controls, matched for race, age, gender and recruiting area. DNA was isolated from whole blood and used for genotyping. PCR products were digested with restriction enzymes and products were analyzed by agarose gel electrophoresis. Odds ratios （OR） and 95% confidence intervals （CI） were estimated. RESULTS： The distribution of the -1195A→G polymorphism was significantly different in esophageal cancer patients compared to controls. The -1195 GG genotype resulted in a higher risk of developing esophageal adenocarcinoma （OR = 3.85, 95% CI： 1.45-10.3） compared with the -1195AA genotype as a reference. The -765 G→C genotype distribution was not different between the two groups. The GG/ GG haplotype was present more often in esophageal adenocarcinoma patients than in controls （OR = 3.45, 95% CI： 1.24-9.58; with AG/AG as a reference）. The same trends were observed in patients with squamous cell carcinomas, however, the results did not reach statistical significance. CONCLUSION： Presence of the COX-2 -1195 GG genotype and of the GG/GG haplotype may result in a higher risk of developing esophageal carcinoma.

关键词： Adenocarcinoma Cyclooxygenase-2 Esophagus Genetic polymorphism Squamous cellcarcinoma

<i>STAT</i>1 gene mutation is not implicated in upper aerodigestive cancers

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Open Journal of Gastroenterology 2012年第2期2卷 68-71页

作者： Polat Dura Rene H. M. te Morsche Martin Lacko Mihai G. Netea Jos W. M. van der Meer Joost P. H. Drenth wilbert H. M. peters Department of Otorhinolaryngology Maastricht University Medical Center Maastricht the Netherlands Departments of Gastroenterology and Internal Medicine Radboud University Nijmegen Medical Center Nijmegen the Netherlands

Autosomal Dominant Chronic Mucocutaneous Candidiasis (AD-CMC) is characterized by defective T cell immunity, leading to fungal infections limited to mucosal surfaces. Recently it was discovered that mutations in the coiled-coil (CC) domain of STAT1 are the cause of AD-CMC. STAT1 deficiency has been implicated in experimental models of oesophageal cancer (EC) and head and neck carcinoma (HNC). Both carcinoma types are prevalent among CMC patients. Consequently, we postulated that the same mutation in the STAT1 gene triggering AD-CMC, could also be involved in oesophageal- or head and neck carcinogenesis. However we failed to identify the c.820C>T mutation in the STAT1 CC domain in 3 cohorts of Dutch Caucasian origin: being 351 EC patients, 325 HNC patients and 309 controls. Although it seems valuable to investigate the relationship between AD-CMC and upper aerodigestive neo- plasms, the c.820C>T mutation in the STAT1 gene does not seem implicated in EC and HNC aetiology.

关键词： STAT1 Signal Transducers and Activators of Transcription Chronic Mucocutaneous Candidiasis Genetic Mutation Esophageal Carcinoma Head and Neck Carcinoma