Disparities of conjugating protective enzyme activities in the colon of patients with adenomas and carcinomas
Disparities of conjugating protective enzyme activities in the colon of patients with adenomas and carcinomas作者机构：Internal Medicine Marien Hospital Department of Gastroenterology Radboud University Nijmegen Medical Center Division of Biostatistics German Cancer Research Center Department of Clinical Pharmacology Technical University of Dresden
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2013年第19卷第36期
页 面：6020-6025页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：Supported by The Technical University of Dresden/Germany
主 题：Glutathione S-transferase UDP-glucuronosyltransferase Detoxification Colon adenoma Colon carcinoma
摘 要：AIM:To investigate the metabolic enzymatic capacity of the colon mucosa to detoxify noxious carcinogenic ***:We investigated the activity of 2 conjugating enzymes-the microsomal uridine glucuronosyltransferase(UGT)and the cytosomal glutathione S-transferase(GST)in the uninvolved mucosa of the colon transversum and sigmoideum in patients with adenomatous polyps and colorectal *** were taken from the mucosa during colonoscopies which were done for clinical(diagnostic)*** storage,the biopsy material was homogenized and after differential centrifugation the enzyme assays were performed with 4-nitrophenol(UGT)and 1-chloro 2,4-dinitrobenzene(GST)as ***:About 48 patients were included of which28 had adenomas and 20 had colorectal carcinomas confirmed by *** activities were expressed as nmol/mg per minute protein for the GST and as pmol/mg per minute protein for the *** of variance(F-test)indicated that both enzymes were more widely distributed in adenoma than in cancer *** means±SD were smaller for cancer patients:GST for adenomas 268±152 vs 241±69 for carcinomas and UGT for adenomas 197±200 vs 150±86 for ***:Compared to patients with adenomatous colon polyps those with colorectal carcinoma exhibited a lower capacity of detoxifying enzyme metabolism and their activities clustered over a smaller range.
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