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Polycyclic aromatic hydrocarbons in benthos of the northern Bering Sea Shelf and Chukchi Sea Shelf

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Journal of Environmental Sciences 2020年第11期32卷 194-199页

作者： Yuxin Ma Yurong Sun Yunkai Li Hongyuan zh.ng wenying Mi School of Oceanography Shanghai Jiao Tong UniversityShanghai 200030China College of Marine Sciences Shanghai Ocean UniversityShanghai 201306China State Key Laboratory of Pollution Control and Resource Reuse College of Environmental Science and EngineeringTongji UniversityShanghai 200092China MINJIE Institute of Environmental Science and Health Research Max-Plank Street 2Geesthacht 21502Germany

Eighteen polycyclic aromatic hydrocarbons(PAHs)were detected in benthos collected onboard the‘Snow Dragon’in the Northern Bering Sea Shelf and Chukchi Sea Shelf during the 6 th Chinese National Arctic Research Expedition(CHINARE 2014).18 PAHs for all biota samples ranged from 34.2 to 128.1 ng/g dry weight(dw),with the highest concentration observed in fish muscle(Boreogadus saida)samples close to *** *** PAH composition pattern was dominated by the presence of lighter 3 ring(57%)and 2 ring(28%)PAHs,indicating oil-related or petrogenic sources as important origins of PAH *** of alkyl-PAHs(1-methylnaphthalene and 2-methylnaphthalene)were lower than their parent PAH(naphthalene)in all biological tissue,and their percentage also decreased significantly(png *** were no significant relationships between PAH concentrations and trophic levels,which is possibly due to the combined results of the complex benthic foodweb in the subarctic/Arctic shelf region,as well as a low assimilation/effective metabolism for *** to toxic potency evaluation results from TCDD toxic equivalents(TEQs)and Ba P-equivalent(Ba PE)values,whelk(Neptunea heros)and starfish(Ctenodiscus crispatus)are two macroinvertebrate species showing relatively higher dioxin-like toxicity and carcinogenic risk.

关键词： Polycyclic aromatic hydrocarbons Arctic shelf Benthos Toxic potency Bioaccumulation

Organophosphate Esters in Air and Seawater of the South China Sea:Spatial Distribution,Transport,and Air−Sea Exchange

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Environment & Health 2023年第3期1卷 191-202页

作者： Lijie Mi zhiyong Xie Lulu zh.ng Joanna J.Waniek Thomas Pohlmann wenying Mi Weihai Xu Institute of Coastal Environmental Chemistry Helmholtz-Zentrum HereonGeesthacht 21502Germany Institute of Oceanography University of HamburgHamburg 20146Germany Key Laboratory of Ocean and Marginal Sea Geology South China Sea Institute of OceanologyChinese Academy of SciencesGuangzhou 510301China Sanya Institute of Ocean Eco-Environmental Engineering Sanya 572000China University of Chinese Academy of Sciences Beijing 100049China Department of Marine Chemistry Leibniz Institute for Baltic Sea Research WarnemündeRostock 18119Germany MINJIE Institute of Environmental Science and Health Research Geesthacht 21502Germany

Organophosphate esters(OPEs)have become one group of chemicals with emerging concern in the marine *** this work,we investigated OPEs in the air and seawater of the South China Sea in summer *** concentrations of∑_(10)OPEs in the atmosphere ranged from 66 to 550 pg/m^(3),with TCIPP,TNBP,TPhP,and TEP predominating in the *** total dissolved OPE concentrations(∑_(10)OPEs without TEP)measured in high-volume water samples ranged from 300 to 3600 pg/L,with a mean concentration of 1180±910 pg/*** was measured with liquid−liquid extraction(LLE),and it showed the highest concentration(average 2000±1450 pg/L)among the selected *** suspended matter associated OPEs accounted for less than 4.7%of the sum of OPE concentrations in *** fractions and air−sea exchange fluxes showed that TCEP,TCIPP,TIBP,TEHP,TPhP,and EHDPP were favored to volatilize,TEP dominated the deposition,while TPrP and TNBP varied between volatilization and *** particle deposition fluxes ranged from 5 to 71 ng/m^(2)/day with an average of 17±15 ng/m^(2)/*** input of∑OPEs to the entire South China Sea via atmospheric particle deposition was estimated to be 22±19 tons/year,while the net air−sea exchange fluxes of OPEs were volatilization from seawater to air with an average of 44±33 tons/*** work suggests that air−sea exchange and atmospheric particle deposition are significant processes interfering with the transport of OPEs in the marine environment.

关键词： Organophosphate esters South China Sea Air−sea exchange Atmospheric deposition Long-range transport

Expression of biologically active human clotting factor Ⅸ(hFⅨ) in the mammary gland of transgenic mice

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Chinese Science Bulletin 1998年第15期43卷 1294-1298页

作者： HUAng Ying zh.ng Kezh.ng HUAng wenying LU Daru HUAng Ying MA zhanlu REN zhaorui QIU Xinfang XUE Jinglun ZEng Yitao HUAng Shuzhen Shanghai Institute of Medical Genetics Shanghai Children’s HospitalShanghai 200040China Institute of Genetics Fudan UniversityShanghai 200433China.

The DNA of human factor Ⅸ (hFⅨ) gene vector pMCⅨm, which had been proven to be able to express in in vitro and living cells, was introduced into 586 zygotes of Kunming White Mice by positive pressure microinjection technique with manual operation. The 499 survival embryos after microinjection were then transferred into pseudopregnant recipient mice and 216 F 0 pups were born. The analysis of PCR and Southern blot hybridization showed that, of the 216, 6 (2 females and 4 males) were integrated with foreign DNA in their genomes, giving an integration frequency of 3% (6/216). Two F\-0 female transgenic mice could express hFⅨ protein in their milk and the content was over 100 ng/mL as measured with ELISA. The biological activities of hFⅨ in the milk of two F\-0 mice were 44 67% and 79 43%, respectively.

关键词： human factorⅨ(hFⅨ) transgenic mice mammary gland expression.

High expression of FABP4 in colorectal cancer and its clinical significance

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Journal of zhejiang University-Science B(Biomedicine & Biotechnology) 2021年第2期22卷 136-145页

作者： Yan zh.ng Wenjia zh.ng Min XIA zhujun XIE Fangmei AN Qiang zhAN wenying TIAN Tianyue zhU Department of Gastroenterology the Second Affiliated Hospital of Nantong UniversityNantong 226001China Department of Gastroenterology Wuxi People's Hospital Affiliated to Nanjing Medical UniversityWuxi 214023China

Objective:To investigate the relationship between the fatty acid-binding protein 4(FABP4)and colorectal cancer(CRC).Methods:Using an enzyme-linked immunosorbent assay(ELISA),we measured the expression of FABP4 in plasma of50 patients who underwent surgery for CRC from October 2017 to May 2018 and 50 healthy *** content of the visceral fat area(VFA)as seen with abdominal computed tomography(CT)scanning was measured by ImageJ *** expression levels of FABP4,E-cadherin,and Snail proteins in CRC and adjacent tissues were determined by ***:The mean concentration of plasma FABP4 of CRC patients was higher than that of the control group(22.46 vs.9.82 ng/m L;Png that FABP4 may promote CRC progression related to epithelial-mesenchymal transition(EMT).

关键词： Fatty acid-binding protein 4(FABP4) Colorectal cancer(CRC) Epithelial-mesenchymal transition

Role of JAK2/STAT3 Pathway in IL-6-Induced Activation of Human Proximal Tubular Epithelial Cells

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器官移植内科学杂志 2009年第4期4卷 181-186页

作者： Linda zhan Hequn Zou Lixin Wei Lin Chen wenying zhou Department of Nephrology The 5th Affiliated Hospital of Sun Yat-sen University Zhuhai 519000 China Department of Nephrology and Rheumatology The 3rd Affiliated Hospital of Southern Medical UniversityGuangzhou 510630 China

Role of JAK2/STAT3 Pathway in IL-6-Induced Activation of Human Proximal Tubular Epithelial Cells

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器官移植内科学杂志 2008年第2期3卷 59-63页

作者： Hequn Zou Linda zhan Lixin Wei Lin Chen wenying zhou Department of Nephrology The 5th Affiliated Hospital of Sun Yat-sen University Zhuhai 519000 China

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垄作玉米免耕播种机破茬装置设计与试验

农业机械学报 2008年第8期39卷 48-53页

作者：孙茸茸李问盈李洪文中国农业大学工学院

针对东北一年一熟垄作区玉米根茬地免耕播种时存在根茬不易破除且易挂茬等问题,设计了一种驱动破茬装置,该装置在3个平面内安装破茬刀具,其中心与开沟器前后对齐。为确定破茬装置的合理结构,设计了3种轴套,同时设计了3种刀具组合方式。... 详细信息

针对东北一年一熟垄作区玉米根茬地免耕播种时存在根茬不易破除且易挂茬等问题,设计了一种驱动破茬装置,该装置在3个平面内安装破茬刀具,其中心与开沟器前后对齐。为确定破茬装置的合理结构,设计了3种轴套,同时设计了3种刀具组合方式。在土槽上进行了三因素三水平的正交试验,通过分析各因素对功率消耗和根茬切断率2个指标的影响,得出较好的参考组合:机具前进速度为0.75km/h,刀轴旋转速度为150r/min,刀具组合方式为zh2。对轴套进行选择的试验表明,当产生相同切土节距S=2cm时,轴套2的功率消耗较低,根茬切断率达到92%。

关键词：玉米免耕播种机垄作破茬机构设计试验

红细胞生成素在新生血管性青光眼中的表达及体外血管诱导形成作用

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中华医学杂志 2016年第46期96卷 3729-3734页

作者：孙怡邢怡桥关文英武汉大学人民医院眼科 430060 内蒙古医科大学附属医院眼科

目的观察红细胞生成素（EPO）在新生血管性青光眼（NVG）中的表达及体外血管诱导形成作用。方法采用酶联免疫吸附法（ELISA）检测2014年6至10月在内蒙古医科大学附属医院眼科就诊的10例NVG患者及10例非NVG患者房水及玻璃体中的EPO水平... 详细信息

目的观察红细胞生成素（EPO）在新生血管性青光眼（NVG）中的表达及体外血管诱导形成作用。方法采用酶联免疫吸附法（ELISA）检测2014年6至10月在内蒙古医科大学附属医院眼科就诊的10例NVG患者及10例非NVG患者房水及玻璃体中的EPO水平。将人脐静脉内皮细胞（HUVECs）作为研究对象，依据EPO浓度分为0.1、1、10 U/ml EPO组及10 ng/ml血管内皮生长因子（VEGF）组处理HUVECs细胞，观察不同浓度的重组人EPO及VEGF对体外HUVECs增殖，血管管腔样结构生成以及毛细血管样结构生成作用的影响，同时采用Western印迹及实时定量PCR（RT－PCR）检测细胞中低氧诱导因子－1α（HIF－1α）蛋白及mRNA表达水平。结果本研究中未见中止试验的患者，非NVG患者眼房水EPO水平为（29.4±0.6）ng/L，而NVG患者房水中EPO含量高达（307.5±7.6）ng/L，二者比较差异有统计学意义（t＝2.186，P＝0.039）；非NVG患者眼玻璃体中EPO水平为（16.7±1.2）ng/L，而NVG患者玻璃体中EPO含量异常增高达到（267.5±11.4）ng/L，二者比较差异有统计学意义（t＝2.517，P＝0.019）。与对照组比较，0.1、1、10 U/ml EPO及10 ng/ml VEGF能显著促进HUVECs增殖，0.1、1、10 U/ml EPO及10 ng/ml VEGF能促进HUVECs体外血管管腔样结构生成以及毛细血管样结构生成，同时降低HIF－1α蛋白及mRNA表达，差异均有统计学意义（均P〈0.05）。结论EPO在NVG中高表达，且外源性EPO能显著促进HUVECs增殖，血管管腔样结构及毛细血管样结构生成，可能与提高HIF－1α表达有关。

关键词：红细胞生成素青光眼,新生血管性血管内皮生长因子血管诱导形成缺氧诱导因子1,α亚基

靶向MAdCAM-1超声造影剂的制备及其体外寻靶实验研究

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南京医科大学学报（自然科学版） 2014年第6期34卷 745-749页

作者：陈文颖郑凯黄敏南京医科大学附属苏州医院超声科江苏苏州215001

目的:制备适用于炎症性肠病超声诊断并靶向MAdCAM-1的长循环脂膜超声造影剂,并鉴定其物理性状及体外寻靶能力。方法:采用超声破碎法制备长循环脂膜超声微泡,通过"生物素-亲和素"法桥接超声微泡与抗MAdCAM-1单克隆抗体(MECA-367)。对制... 详细信息

目的:制备适用于炎症性肠病超声诊断并靶向MAdCAM-1的长循环脂膜超声造影剂,并鉴定其物理性状及体外寻靶能力。方法:采用超声破碎法制备长循环脂膜超声微泡,通过"生物素-亲和素"法桥接超声微泡与抗MAdCAM-1单克隆抗体(MECA-367)。对制备出的靶向微泡造影剂进行物理性状检测;通过光镜和激光共聚焦显微镜观察该靶向造影剂对TNF-α诱导的炎症性肠病细胞模型的结合能力。同法制备携同型对照抗体IgG2a微泡作对照。结果:制备的靶向超声微泡形态好且粒径较均匀,平均粒径(464.5±85.7)nm,Zeta电位(-19.3±2.5)mV。SVEC4-10细胞MAdCAM-1的表达与TNF-α刺激时间成正相关。并且,当TNF-α浓度达到20 ng/ml时,MAdCAM-1的表达达到峰值。体外寻靶试验表明,该靶向造影剂较多并牢固地黏附到表达MAdCAM-1的细胞周围;携IgG2a的同型对照组未见明显结合。结论:成功制备出桥连MECA-367的靶向长循环脂膜超声造影剂,该造影剂在体外对高表达MAdCAM-1的炎性细胞模型有较强的特异性亲和力;TNF-α诱导SVEC4-10细胞建立炎症性肠病细胞模型的方法简便易行。

关键词：靶向超声造影剂 MAdCAM-1 炎症性肠病细胞模型