Expression of biologically active human clotting factor Ⅸ(hFⅨ) in the mammary gland of transgenic mice

作     者：HUANG Ying ZHANG Kezhong HUANG Wenying LU Daru HUANG Ying MA Zhanlu REN Zhaorui QIU Xinfang XUE Jinglun ZENG Yitao HUANG Shuzhen 

作者机构：Shanghai Institute of Medical GeneticsShanghai Children’s HospitalShanghai 200040China Institute of GeneticsFudan UniversityShanghai 200433China. 

出 版 物：《Chinese Science Bulletin》 (科学通报(英文版))

年 卷 期：1998年第43卷第15期

页      面：1294-1298页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：China’s"86 3"High_TechPlan (GrantNo .10 1_05_0 4_01) ShanghaiMunicipalScientificFoundationProject(GrantNo .95 5 4 190 0 1) 

主　　题：human factorⅨ(hFⅨ) transgenic mice mammary gland expression. 

摘      要：The DNA of human factor Ⅸ (hFⅨ) gene vector pMCⅨm, which had been proven to be able to express in in vitro and living cells, was introduced into 586 zygotes of Kunming White Mice by positive pressure microinjection technique with manual operation. The 499 survival embryos after microinjection were then transferred into pseudopregnant recipient mice and 216 F 0 pups were born. The analysis of PCR and Southern blot hybridization showed that, of the 216, 6 (2 females and 4 males) were integrated with foreign DNA in their genomes, giving an integration frequency of 3% (6/216). Two F\-0 female transgenic mice could express hFⅨ protein in their milk and the content was over 100 ng/mL as measured with ELISA. The biological activities of hFⅨ in the milk of two F\-0 mice were 44 67% and 79 43%, respectively.