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Genes & Diseases 2024年第2期11卷 575-578页

作者： Qianling Wang Yiyin Zhang wenxi ding Cheng Feng Yuyan Wang Xiaoli Wei Ziting Qu Hui Wang Xiaoying Liu Hua Wang Kangsheng Gu Department of Oncology The First Affiliated Hospital of Anhui Medical UniversityHefeiAnhui 230022China School of Life Sciences Anhui Medical UniversityHefeiAnhui 230032China

Neutrophils constitute a significant portion of the immune cells present within the tumor *** generated by our group has confirmed neutrophils to be an adverse independent prognostic factor affecting the disease-free survival of gastric carcinoma(GC)patients.1 Neutrophil extracellular traps(NETs),protein-covered DNA webs that interact with tumor cells in the tumor microenvironment,were detected in GC tissues and found to clinically associated with disease progression in our previous study.2 NETosis could be mediated by a series of agonists,such as interleukin 8(IL-8).3 However,the mechanism of IL-8 in the progression of GC occurs via NETs remains *** this study,we identified that IL-8-mediated NETosis can promote the proliferation,migration,and invasion of GC cells in vitro,which can be abrogated by NET degradation through deoxyribonucleaseI(DNase I)or IL-8 suppression through neutralizing *** of NETs by DNase I or blockade of the IL-8-CXC chemokine receptor 2(CXCR2)axis could result in growth retardation in mouse *** also confirmed that NETs may influence the biological behavior of GC cells by up-regulating the expression of transcription factor IB-related factor 1(BRF1)and cyclin p21/p27.

关键词： invasion gastric progression

胸膜肺炎放线杆菌ApxⅠ蛋白单克隆抗体制备和抗原表位初步鉴定

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畜牧与兽医 2024年第5期56卷 119-126页

作者：蔡金双耿琰张宝戈车巧林丁文傒李玉峰南京农业大学/农业部动物细菌学重点开放实验室江苏南京210095 江苏南农高科技股份有限公司江苏无锡214400

为了制备胸膜肺炎放线杆菌(Actinobacillus pleuropneumoniae,APP)Ⅰ型ApxⅠ毒素(ApxⅠ)AI2蛋白特异性单克隆抗体,进而建立用于评估亚单位疫苗免疫效果的阻断ELISA方法,以实验室前期筛选得到的ApxⅠ抗原优势决定簇AI2重组蛋白为免疫原,... 详细信息

为了制备胸膜肺炎放线杆菌(Actinobacillus pleuropneumoniae,APP)Ⅰ型ApxⅠ毒素(ApxⅠ)AI2蛋白特异性单克隆抗体,进而建立用于评估亚单位疫苗免疫效果的阻断ELISA方法,以实验室前期筛选得到的ApxⅠ抗原优势决定簇AI2重组蛋白为免疫原,免疫6周龄BALB/c雌鼠。利用常规细胞融合和间接ELISA筛选阳性杂交瘤细胞,获得4株单克隆抗体,命名为2C2、4E4、5E7和6F2,上清液效价分别为1∶6400、1∶6400、1∶12800和1∶6400。ELISA与Western blot结果表明,制备的4株单克隆抗体均与重组AI2蛋白有良好的反应性。其中5E7可与APP天然ApxⅠ毒素蛋白特异性结合,同时4株单克隆抗体均不与多杀性巴氏杆菌、支气管败血波氏菌、猪丹毒丝菌、副猪格拉菌和猪链球菌2型反应。亚类分型结果表明,2C2、4E4、5E7属于IgG1亚类,6F2属IgG2a亚类,4株单抗轻链均为κ链。通过构建AI2重组蛋白截短体,初步确定线性表位在74~93 aa之间。本研究成功制备了4株针对AI2蛋白单克隆抗体,为APP亚单位疫苗免疫效果评估方法的建立奠定了基础。

关键词：胸膜肺炎放线杆菌 AI2蛋白单克隆抗体抗原表位

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High VHL Expression Reverses Warburg Phenotype and Enhances Immunogenicity in Kidney Tumor Cells

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Genomics, Proteomics & Bioinformatics 2022年第4期20卷 657-669页

作者： Songbiao Zhu wenxi ding Yuling Chen Weixuan Wang Renhua Xu Chongdong Liu Xiaohui Liu Haiteng Deng MOE Key Laboratory of Bioinformatics Center for Synthetic and Systematic BiologySchool of Life SciencesTsinghua UniversityBeijing 100084China Guangdong Metabolic Diseases Research Center of Integrated Chinese and Western Medicine Guangdong Pharmaceutical UniversityGuangzhou 510006China School of Nursing Binzhou Medical UniversityYantai 264003China Department of Obstetrics and Gynecology Beijing Chao-Yang HospitalCapital Medical UniversityBeijing 100020China

Clear cell renal cell carcinoma(ccRCC)is a frequently occurring renal *** Von Hippel-Lindau disease tumor suppressor VHL,a known tumor suppressor gene,is frequently mutated in about 50%of patients with ***,it is unclear whether VHL influences the progression of ccRCC tumors expressing wild-type *** the present study,we found that higher expression of VHL was correlated with the better disease-free survival(DFS)in ccRCC patients using The Cancer Genome Atlas(TCGA)*** revealed that VHL overexpression in ccRCC cells inhibited epithelial-mesenchymal transition(EMT),sterol regulatory elementbinding protein 1(SREBP1)-regulated triglyceride synthesis,and cell *** analysis provided us a global view that VHL regulated four biological processes,including metabolism,immune regulation,apoptosis,and cell ***,we found that VHL overexpression led to up-regulated expression of proteins associated with antigen processing and interferonresponsive proteins,thus rendering ccRCC cells more sensitive to interferon *** defined an interferon-responsive signature(IRS)composed of ten interferon-responsive proteins,whose m RNA expression levels were positively correlated with DFS in ccRCC *** together,our results propose that the subset of ccRCC patients with high VHL expression benefit from immunotherapy.

关键词： Renal cancer VHL Immunogenicity Multi-omics Interferon

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中国水资源可持续发展的对策与建议

中国农学通报 2011年第14期27卷 221-226页

作者：丁文喜中共郑州市委党校郑州450042

为进一步促进水资源可持续发展,笔者通过对中国水资源现状的调研分析,借鉴发达国家经验和前人研究成果,以系统性、前瞻性和综合性为视角,将水资源的开发、保护、管理和应用等与水资源立法、行政管理、科技创新、理念转变、全民行为等有... 详细信息

为进一步促进水资源可持续发展,笔者通过对中国水资源现状的调研分析,借鉴发达国家经验和前人研究成果,以系统性、前瞻性和综合性为视角,将水资源的开发、保护、管理和应用等与水资源立法、行政管理、科技创新、理念转变、全民行为等有机融合,提出了促进水资源可持续发展的对策和建议。笔者认为,进一步加快水资源立法进程、规范和完善管理机制,依靠科技进步,把握科学发展理念,促进水资源开发与管理水平升级,是实现水资源可持续利用,支撑社会经济可持续发展的基本要求和制度保证;不断改善水环境条件和用水理念,倡导"建设节约型社会",以"节流、控污、开源"应对水资源发展,采取一系列措施,实现全民行动,营造一个全社会对水资源高效利用与科学管理的氛围,这是促进水资源可持续利用,长期惠及世界生灵的有效手段和根本保证;当制度和行为变为高度统一,综合手段同步发挥作用时,水资源才会真正富有涵养,水资源才会体现出人们科学开发、高效利用、系统治理和有效保护的真正效能。这是中国治水策略重大调整的核心和关键。

关键词：水资源可持续发展对策建议

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现代农业与可持续发展的生态农业

中国农学通报 2010年第15期26卷 328-331页

作者：丁文喜郑州市委党校工商管理教研室郑州450042

现代农业的发展并不仅仅是追求经济效益,还要建设良好的生态系统,实现人与自然的和谐发展。中国正面临来自人口、资源、生态环境的恶化等多重挑战,必须按照科学发展观的思想,坚持走可持续发展道路;必须正确处理经济与人口、资源、环境... 详细信息

现代农业的发展并不仅仅是追求经济效益,还要建设良好的生态系统,实现人与自然的和谐发展。中国正面临来自人口、资源、生态环境的恶化等多重挑战,必须按照科学发展观的思想,坚持走可持续发展道路;必须正确处理经济与人口、资源、环境的关系,达到经济、社会和生态的协调发展。因此,吸收借鉴国外生态农业发展的经验,根据新农村建设可持续发展的要求,形成适合中国国情的现代生态农业运行模式显得尤为重要。

关键词：现代农业可持续发展生态农业

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构建有中国特色的企业文化

中共郑州市委党校学报 2002年第5期 32-33页

作者：丁文喜中共郑州市委党校

文化是人类在社会经济活动中长期积累起来的精神财富。企业管理活动与特定的文化相融合而形成企业文化。要构建有中国特色的企业文化,必须继承和弘扬中华民族传统文化中的优秀遗产,选择适合中国国情的企业文化模式,以使中国企业的发展... 详细信息

文化是人类在社会经济活动中长期积累起来的精神财富。企业管理活动与特定的文化相融合而形成企业文化。要构建有中国特色的企业文化,必须继承和弘扬中华民族传统文化中的优秀遗产,选择适合中国国情的企业文化模式,以使中国企业的发展跟上世界步伐。

关键词：企业文化构建企业模式中国特色

Protective immunity induced by Eimeria common antigen 14-3-3 against Eimeria tenella,Eimeria acervulina and Eimeria maxima

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Protective immunity induced by Eimeria common antigen 14-3-3...

Toxoplasma gondii Proteasome Subunit Alpha Type 1 with Chitosan:A Promising Alternative to Traditional Adjuvant

中国畜牧兽医学会兽医寄生虫学分会第一届青年科学家学术论坛

作者： Jianhua Liu wenxi ding Lianrui Liu Lingjuan Li Di Tian Wenyu Li Lixin Xu Ruofeng Yan Xiangrui Li 宋小凯 College of Veterinary Medicine Nanjing Agricultural University

Background:Avian coccidiosis is often caused by co-infection with several species of Eimeria *** a multivalent vaccine with an antigen common to multiple Eimeria species is a promising strategy for controlling clinical common co-infection of *** the previous study,14-3-3 was identified as one of the immunogenic common antigen in ***,E acervulina and *** aim of the present study was to evaluate the immunogenicity and protective efficacy of Ea14-3-3 in the form of DNA vaccine against infection with three species of Eimeria both individually and ***:After vaccination with pVAX-Ea14-3-3,the Ea14-3-3 gene was transcribed and expressed in the injected *** with pVAX-Ea14-3-3 significantly increased the proportion of CD4and CD8T lymphocytes and produced a strong IgY response in immunized ***,pVAX-Ea14-3-3 stimulated the chicken’s splenocytes to produce high levels of Th1-type(IFN-γ,IL-2) and Th2-type(IL-4)*** vaccine-induced immune response was responsible to increase weight gain,decreased the oocyst output,and alleviated enteric lesions significantly in immunized chickens as compared to control group,in addition to induce moderate anti-coccidial index(ACI).Conclusion:These results indicate that Ea14-3-3 is highly immunogenic and capable to induce significant immune ***,Ea14-3-3 antigen can provide effective protection against infection with Eimeria tenella,Eimeria acervulina,Eimeria maxima both individually and in combination with three Eimeria *** outcomes of our study provide an effective candidate antigen for developing a multivalent Eimeria vaccine against mixed infection with various Eimeria species under natural conditions.

关键词： Chicken coccidian Common antigen 14-3-3 Mixed infection Immunogenicity DNA vaccine

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Toxoplasma gondii Proteasome Subunit Alpha Type 1 with Chito...

2021中国畜牧兽医学会兽医寄生虫学分会第二届青年科学家学术论坛

作者： ZhengQing Yu wenxi ding Muhammad Tahir Aleem JunZhi Su JunLong Liu JianXun Luo RuoFeng Yan LiXin Xu XiaoKai Song XiangRui Li MOE Joint International Research Laboratory of Animal Health and Food Safety College of Veterinary MedicineNanjing Agricultural University State Key Laboratory of Veterinary Etiological Biology Key Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences

As an important zoonotic protozoan,Toxoplasma gondii(***) has spread around the world,leading to infections in one-third of the *** is still no effective vaccine or medicine against *** recombinant antigens entrapped within nanospheres have benefits over traditional *** the present study,we first expressed and purified *** proteasome subunit alpha type 1(TgPSAl),then encapsulated the recombinant TgPSAl(rTgPSA1) in chitosan nanospheres(CS nanospheres,rTgPSA1/CS nanospheres) and incomplete Freund’s adjuvant(IFA,rTgPSA1/IFA emulsion).Antigens entrapped in CS nanospheres reached an encapsulation efficiency of 67.39%,and rTgPSA1/CS nanospheres showed a more stable release profile compared to rTgPSA1/IFA emulsion in *** vivo,Thl-biased cellular and humoral immune responses were induced in mice and chickens immunized with rTgPSA1/CS nanospheres and rTgPSA1/IFA emulsion,accompanied by promoted production of antibodies,IFN-γ,IL-4,and IL-17,and modulated production of *** with rTgPSA1/CS nanospheres and rTgPSA1/IFA emulsion conferred significant protection,with prolonged survival time in mice and significantly decreased parasite burden in ***,our results also indicate that rTgPSA1/CS nanospheres could be used as a substitute for rTgPSA1/IFA emulsion,with the optimal administration route being intramuscular in mass ***,the results of this study indicate that rTgPSA1/CS nanospheres represent a promising vaccine to protect animals against acute toxoplasmosis.

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水煤浆管道爆裂事故管道失效原因分析

工业安全与环保 2011年第1期37卷 49-50页

作者：杨一张礼敬丁文溪南京工业大学城市建设与安全工程学院南京21009 兰州石化职业技术学院兰州730060

针对一起20G水煤浆管道爆裂事故,采用宏观形貌分析、化学成分分析、金相显微组织分析、SEM微观形貌分析、力学性能测试等试验方法,分析了水煤浆管道失效的原因。结果表明,管道破裂是由于管内压力突跃导致,薄膜方程估算得爆炸压力应在200... 详细信息

针对一起20G水煤浆管道爆裂事故,采用宏观形貌分析、化学成分分析、金相显微组织分析、SEM微观形貌分析、力学性能测试等试验方法,分析了水煤浆管道失效的原因。结果表明,管道破裂是由于管内压力突跃导致,薄膜方程估算得爆炸压力应在200MPa以上,推测为工艺过程中物理化学变化引起的。

关键词：水煤浆管道失效分析压力突跃薄膜理论

T300/AG80复合材料U形结构件成型残余应力的有限元模拟

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机械工程材料 2021年第2期45卷 85-89页

作者：杨康闫照为梁宇王吉丁文喜沈阳航空航天大学辽宁省通用航空重点实验室沈阳110136 辽宁通用航空研究院设计部沈阳110136

利用ABAQUS有限元软件建立T300碳纤维增强AG80环氧树脂(T300/AG80)复合材料U形薄壁结构件模型,研究了热压固化成型过程中的应力和回弹变形量(同步模拟脱模)变化以及脱模后的残余应力分布,通过小孔试验法对残余应力模拟结果进行了验证。... 详细信息

利用ABAQUS有限元软件建立T300碳纤维增强AG80环氧树脂(T300/AG80)复合材料U形薄壁结构件模型,研究了热压固化成型过程中的应力和回弹变形量(同步模拟脱模)变化以及脱模后的残余应力分布,通过小孔试验法对残余应力模拟结果进行了验证。结果表明:成型过程中,U形结构件侧壁和底面的应力和回弹变形量均随时间延长而增加;随着距底面距离或距对称中心距离的增大,应力和回弹变形量增大;脱模后,U形结构件对称中心处的残余应力最小,残余应力随着距底面距离或距对称中心距离的增大而增大;脱模前后释放的残余应力越大,回弹变形量越大;小孔法测得的残余应力与模拟结果的相对误差小于10%,说明模拟结果较准确。

关键词：树脂基复合材料固化成型残余应力 ABAQUS软件小孔法