Toxoplasma gondii Proteasome Subunit Alpha Type 1 with Chitosan:A Promising Alternative to Traditional Adjuvant
作者单位：MOE Joint International Research Laboratory of Animal Health and Food SafetyCollege of Veterinary MedicineNanjing Agricultural University State Key Laboratory of Veterinary Etiological BiologyKey Laboratory of Veterinary Parasitology of Gansu ProvinceLanzhou Veterinary Research InstituteChinese Academy of Agricultural Sciences
会议名称：《2021中国畜牧兽医学会兽医寄生虫学分会第二届青年科学家学术论坛》
会议日期：2021年
学科分类：090601[农学-基础兽医学] 09[农学] 0906[农学-兽医学]
摘 要：As an important zoonotic protozoan,Toxoplasma gondii(***) has spread around the world,leading to infections in one-third of the *** is still no effective vaccine or medicine against *** recombinant antigens entrapped within nanospheres have benefits over traditional *** the present study,we first expressed and purified *** proteasome subunit alpha type 1(TgPSAl),then encapsulated the recombinant TgPSAl(rTgPSA1) in chitosan nanospheres(CS nanospheres,rTgPSA1/CS nanospheres) and incomplete Freund’s adjuvant(IFA,rTgPSA1/IFA emulsion).Antigens entrapped in CS nanospheres reached an encapsulation efficiency of 67.39%,and rTgPSA1/CS nanospheres showed a more stable release profile compared to rTgPSA1/IFA emulsion in *** vivo,Thl-biased cellular and humoral immune responses were induced in mice and chickens immunized with rTgPSA1/CS nanospheres and rTgPSA1/IFA emulsion,accompanied by promoted production of antibodies,IFN-γ,IL-4,and IL-17,and modulated production of *** with rTgPSA1/CS nanospheres and rTgPSA1/IFA emulsion conferred significant protection,with prolonged survival time in mice and significantly decreased parasite burden in ***,our results also indicate that rTgPSA1/CS nanospheres could be used as a substitute for rTgPSA1/IFA emulsion,with the optimal administration route being intramuscular in mass ***,the results of this study indicate that rTgPSA1/CS nanospheres represent a promising vaccine to protect animals against acute toxoplasmosis.