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Science Bulletin 2020年第17期65卷 1436-1439,M0003页

作者： Xinjie wang Mingtian Zhong Yong Liu Peixiang Ma Lu Dang Qingzhou Meng wenwei wan Xiaodong Ma Jia Liu Guang Yang Zifeng Yang Xingxu Huang Ming Liu Shanghai Institute for Advanced Immunochemical Studies ShanghaiTech UniversityShanghai 201210China Institute for Brain Research and Rehabilitation Guangdong Key Laboratory of Mental Health and Cognitive ScienceCenter for Studies of Psychological ApplicationSouth China Normal UniversityGuangzhou 510631China State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseGuangzhou Institute of Respiratory HealthThe First Affiliated HospitalGuangzhou Medical UniversityGuangzhou 510120China Kingmed Virology Diagnostic&Translational Center Guangzhou Kingmed Center for Clinical Laboratory Co.Ltd.Guangzhou 510005China Affiliated Cancer Hospital&Institute of Guangzhou Medical University Guangzhou 510095China School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China

The outbreak of the 2019 novel coronavirus disease(COVID-19)has had a significant impact on global *** advance the diagnostic method of COVID-19,we developed a CRISPR/Cas12a-based assay with a naked eye readout,CRISPR/*** assay can detect as few as 10 copies of the virus gene in 45 min without a special instrument and has good consistency with the qPCR assay,providing a simple and reliable on-site diagnostic method suitable for a local hospital or community testing.

关键词：公共卫生安全控制疫情隔离防护灵敏检测荧光法致病病原体社区医院诊断方法

Are bone marrow regenerative cells ideal seed cells for the treatment of cerebral ischemia?

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Neural Regeneration Research 2013年第13期8卷 1201-1209页

作者： Yi Li Xuming Hua Fang Hua wenwei Mao Liang wan Shiting Li Department of Neurosurgery Xinhua Hospital Shanghai Jiao Tong University School of Medicine Department of Emergency Medicine Brain Research Laboratory Emory University School of Medicine Laboratory of Microbiology and Biochemical Pharmaceutics School of Pharmacy Shanghai Jiao Tong University

Bone marrow cells for the treatment of ischemic brain injury may depend on the secretion of a large number of neurotrophic factors. Bone marrow regenerative cells are capable of increasing the secretion of neurotrophic factors. In this study, after tail vein injection of 5-fluorouracil for 7 days, bone marrow cells and bone marrow regenerative cells were isolated from the tibias and femurs of rats, and then administered intravenously via the tail vein after focal cerebral ischemia. Immunohistological staining and reverse transcription-PCR detection showed that transplanted bone marrow cells and bone marrow regenerative cells could migrate and survive in the ischemic regions, such as the cortical and striatal infarction zone. These cells promote vascular endothelial cell growth factor mRNA expression in the ischemic marginal zone surrounding the ischemic penumbra of the cortical and striatal infarction zone, and have great advantages in promoting the recovery of neurological function, reducing infarct size and promoting angiogenesis. Bone marrow regenerative cells exhibited stronger neuroprotective effects than bone marrow cells. Our experimental findings indicate that bone marrow regenerative cells are preferable over bone marrow cells for cell therapy for neural regeneration after cerebral ischemia. Their neuroprotective effect is largely due to their ability to induce the secretion of factors that promote vascular regeneration, such as vascular endothelial growth factor.

关键词： neural regeneration brain injury cerebral ischemia seed cells bone marrow transplantation bonemarrow cells bone marrow regenerative cells vascular regeneration factor brain neuroregeneration

Effect of Wuda granule on gastrointestinal function recovery after laparoscopic intestinal resection:a randomized–controlled trial

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Gastroenterology Report 2022年第1期10卷 257-264页

作者： Haiping Zeng Wei wang Lixing Cao Yuyan Wu wenwei Ouyang Dechang Diao Jin wan Qicheng Chen Zhiqiang Chen The Second School of Clinical Medicine Guangzhou University of Chinese MedicineGuangzhouGuangdongP.R.China Center of TCM applications Perioperative Guangdong Provincial Hospital of Chinese MedicineGuangzhouGuangdongP.R.China Department of Gastrointestinal Surgery Guangdong Provincial Hospital of Chinese MedicineGuangzhouGuangdongP.R.China Key Unit of Methodology in Clinical Research The Second Affiliated Hospital of Guangzhou University of Chinese MedicineGuangzhouGuangdongP.R.China Department of Global Public Health Karolinska InstituteStockholmSweden Department of Colorectal Surgery Guangdong Provincial Hospital of Chinese MedicineGuangzhouGuangdongP.R.China

Background Previous studies have suggested that the Wuda granule(WDG)could promote the recovery of gastrointestinal(GI)function after gynecologic abdominal *** trial aimed to investigate the efficacy and safety of WDG in the rapid recovery of GI function in patients after laparoscopic intestinal resection in the setting of enhanced recovery after surgery(ERAS)-based perioperative *** We performed a randomized,double-blind,placebo-controlled pilot *** patients who met the inclusion criteria were randomly assigned to either the WDG group or the placebo group in a 1:1 *** patients received WDG or placebo twice a day in addition to ERAS-based perioperative care,starting on post-operative Day 1 until Day *** primary outcomes were time to first bowel movement and time to first tolerance of solid *** secondary outcomes were time to first flatus,length of hospital stay(LOS),and post-operative ileus-related *** events were also *** There were no statistically significant differences in baseline characteristics between the two *** median time to first bowel movement was significantly decreased in the WDG group compared with the control group(27.6 vs 50.1 h;P0.05)and LOS(5.0 vs 5.0 days;P>0.05)were not statistically *** occurrence rates of post-operative nausea,vomiting,abdominal distension,and abdominal pain were similar in the two *** adverse events occurred in any *** The addition of WDG to ERAS post-operative care after laparoscopic intestinal resection can safely promote the rapid recovery of GI function.

关键词： Wuda granule laparoscopic intestinal resection post-operative gastrointestinal function ERAS

Brain regulates weight bearing bone through PGE2 skeletal interoception: implication of ankle osteoarthritis and pain

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Bone Research 2024年第1期12卷 107-121页

作者： Feng Gao Qimiao Hu wenwei Chen Jilong Li Cheng Qi Yiwen Yan Cheng Qian Mei wan James Ficke Junying Zheng Xu Cao Department of Orthopedic Surgery Johns Hopkins University School of MedicineBaltimoreMaryland 21205USA Department of Biomedical Engineering Johns Hopkins University School of MedicineBaltimoreMaryland 21205USA

Bone is a mechanosensitive tissue and undergoes constant remodeling to adapt to the mechanical loading ***,it is unclear whether the signals of bone cells in response to mechanical stress are processed and interpreted in the *** this study,we found that the hypothalamus of the brain regulates bone remodeling and structure by perceiving bone prostaglandin E2(PGE2)concentration in response to mechanical *** PGE2 levels are in proportion to their weight *** weight bearing changes in the tail-suspension mice,the PGE2 concentrations in bones change in line with their weight bearing *** of cyclooxygenase-2(COX2)in the osteoblast lineage cells or knockout of receptor 4(EP4)in sensory nerve blunts bone formation in response to mechanical ***,knockout of TrkA in sensory nerve also significantly reduces mechanical load-induced bone ***,mechanical loading induces cAMP-response element binding protein(CREB)phosphorylation in the hypothalamic arcuate nucleus(ARC)to inhibit sympathetic tyrosine hydroxylase(TH)expression in the paraventricular nucleus(PVN)for ***,we show that elevated PGE2 is associated with ankle osteoarthritis(AOA)and ***,our data demonstrate that in response to mechanical loading,skeletal interoception occurs in the form of hypothalamic processing of PGE2-driven peripheral signaling to maintain physiologic bone homeostasis,while chronically elevated PGE2 can be sensed as pain during AOA and implication of potential treatment.

关键词： pain elevated PGE2

OsPKpa_1 encodes a plastidic pyruvate kinase that affects starch biosynthesis in the rice endosperm

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Journal of Integrative Plant Biology 2018年第11期60卷 1097-1118页

作者： Yue Cai wenwei Zhang Jie Jin Xiaoming Yang Xiaoman You Haigang Yan Liang wang Jie Chen Jiahuan Xu Weiwei Chen Xingang Chen Jing Ma Xiaojie Tang Fei Kong Xiaopin Zhu Guoxiang wang Ling Jiang William Terzaghi Chunming wang Jianmin wan State Key Laboratory for Crop Genetics and Germplasm Enhancement Jiangsu Plant Gene Engineering Research CenterNanjing AgriculturalUniversityNanjing 210095China National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop ScienceChinese Academy of AgriculturalSciencesBeijing 100081China Department of Biology Wilkes UniversityWilkes-BarrePA 18766USA

Pyruvate kinase （PK） is a key enzyme in glycolysis and carbon metabolism. Here, we isolated a rice （Oryza sativa） mutant, w59, with a white-core floury endosperm. Map-based cloning of w59 identified a mutation in OsPKpα1, which encodes a plastidic isoform of PK （PKp）. OsPKpα1 localizes to the amyloplast stroma in the developing endosperm, and the mutation of OsPKpα1 in w59 decreases the plastidic PK activity, resulting in dramatic changes to the lipid biosynthesis in seeds. The w59 grains were also characterized by a marked decrease in starch content. Consistent with a decrease in number and size of the w59 amyloplasts, large empty spaces were observed in the central region of the w59 endosperm, at the early grain-filling stage. Moreover, a phylogenetic analysis revealed four potential rice isoforms of OsPKp. We validated the in vitro PK activity of these OsPKps through reconstituting active PKp complexes derived from inactive individual OsPKps, revealing the heteromeric structure of rice PKps, which was further confirmed using a protein- protein interaction analysis. These findings suggest a functional connection between lipid and starch synthesis in rice endosperm amyloplasts.

关键词： Figure OsPKpa1 encodes a plastidic pyruvate kinase that affects starch biosynthesis in the rice endosperm

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考虑滑移的钢-混凝土组合梁有限单元法

东南大学学报（自然科学版） 2007年第2期37卷 206-212页

作者：王文炜翁昌年万水李淑琴东南大学交通学院南京210096 辽宁省大通公路工程有限公司沈阳116021

利用考虑滑移的钢-混凝土组合梁单元,建立了考虑滑移的钢-混凝土组合梁有限单元法.在粘结剪力和滑移微分控制方程的基础上,建立了关于组合梁单元杆端未知力的力法方程.在力法方程的基础上,给出了组合梁单元的刚度矩阵、杆端位移向量及... 详细信息

利用考虑滑移的钢-混凝土组合梁单元,建立了考虑滑移的钢-混凝土组合梁有限单元法.在粘结剪力和滑移微分控制方程的基础上,建立了关于组合梁单元杆端未知力的力法方程.在力法方程的基础上,给出了组合梁单元的刚度矩阵、杆端位移向量及杆端荷载向量并建立了刚度方程.为验证有限单元法的正确性,对试验梁进行了跨中挠度、沿梁高应变分布及梁端滑移的计算分析.计算结果表明,所建立的考虑滑移的钢-混凝土组合梁有限单元法与试验值吻合较好,计算结果可靠.

关键词：滑移钢-混凝土组合梁有限单元法刚度矩阵位移向量荷载向量

Effects of Slow-release Nitrogen Fertilizer on Yield and Nitrogen Accumulation of Summer Maize in Shajiang Black Soil Area

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Agricultural Biotechnology 2024年第2期13卷 72-74页

作者： Yongfeng XING Changmin WEI Guoli CHEN Weimeng XU wanyou SONG Guizhi LI wenwei ZHOU Yanwei wan Enzhong ZHOU Weifang LI Zhoukou Academy of Agricultural Sciences Zhoukou 466000China

[Objectives] This study was conducted to verify the field application effect of slow-release nitrogen fertilizer on summer maize in Shajiang black soil area by simultaneous sowing and fertilization, and explore the application scope and nitrogen metabolism mechanism, so as to lay a foundation for fertilizer reduction and efficiency improvement. [Methods] With maize variety Beiqing 340 and sulfur-coated urea as experimental materials, five nitrogen application levels were set, namely, control (C0), slow-release nitrogen 70 kg/hm^(2) (C70), slow-release nitrogen 140 kg/hm^(2) (C140), slow-release nitrogen 210 kg/hm^(2) (C210) and slow-release nitrogen 280 kg/hm^(2) (C280). The phosphorus and potassium fertilizers were all in accordance with the unified standard. [Results] With the application rate of slow-release nitrogen increasing, the nitrogen accumulation in organs increased first and then decreased after tasseling stage of maize. In order to reduce the fertilizing amount and increase efficiency, 210 kg/hm^(2) of slow-release nitrogen fertilizer was the best fertilizing amount for summer maize in Shajiang black soil area. [Conclusions] This study provides reference for fertilizer reduction, efficiency improvement and sustainable development of summer maize in Shajiang black soil area.

关键词： Slow-release nitrogen fertilizer Shajiang black soil Summer maize Nitrogen metabolism Yield

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深圳市乘用车道路行驶工况构建

深圳大学学报（理工版） 2016年第3期33卷 281-287页

作者：万霞黄文伟强明明长安大学环境科学与工程学院陕西西安710064 深圳职业技术学院汽车与交通学院广东深圳518055

选取深圳市代表性测试路线,采用平均车流法,对测试车辆车载GPS连续20 d、每天3个采样时间段进行数据采集,累计行驶里程达6 022 km.用短行程分析法构建深圳市城区乘用车行驶工况.结果显示,深圳市城区乘用车平均车速为28.63 km/h,匀速工... 详细信息

选取深圳市代表性测试路线,采用平均车流法,对测试车辆车载GPS连续20 d、每天3个采样时间段进行数据采集,累计行驶里程达6 022 km.用短行程分析法构建深圳市城区乘用车行驶工况.结果显示,深圳市城区乘用车平均车速为28.63 km/h,匀速工况占时间比例为24.4%,加速和减速工况所占时间两者之和为52.9%,与国内其他城市相比,表现出较好的车辆排放工况.与欧美日标准工况相比,深圳市的平均车速比欧洲NEDC、美国FTP75、日本Japan15标准工况分别低14.8%、16.3%和15.5%,在加速、减速和各工况所占时间比例等其他参数上也有较大的差距.

关键词：公路运输行驶工况平均车流法短行程乘用车城市道路

钢-混凝土组合梁混凝土收缩徐变的增量微分方法

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东南大学学报（自然科学版） 2010年第6期40卷 1252-1256页

作者：王文炜何初生冯竹林万水翁昌年东南大学交通学院南京210096 辽宁大通公路工程有限公司沈阳110179

为深入了解混凝土收缩徐变对钢-混凝土组合梁力学性能的影响,采用随时间变化的换算弹性模量法建立了钢-混凝土组合梁混凝土收缩徐变的增量微分模型,得到了组合梁内力、挠度和钢-混凝土界面滑移的微分控制方程.根据边界条件,给出了内力... 详细信息

为深入了解混凝土收缩徐变对钢-混凝土组合梁力学性能的影响,采用随时间变化的换算弹性模量法建立了钢-混凝土组合梁混凝土收缩徐变的增量微分模型,得到了组合梁内力、挠度和钢-混凝土界面滑移的微分控制方程.根据边界条件,给出了内力、变形及钢-混凝土界面滑移等各项力学性能指标的闭合解.为验证增量微分方法的正确性,对试验梁和算例梁进行了跨中挠度-时间曲线、沿梁高应变分布及梁端钢-混凝土界面滑移的计算分析.计算结果表明,所建立的增量微分法与试验值和其他计算方法的计算结果吻合较好,可以有效地预测钢-混凝土组合梁的长期力学性能.

关键词：钢-混凝土组合梁混凝土收缩徐变增量微分法

基于SCR系统的公交车颗粒物排放特性的道路实验研究

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环境工程学报 2016年第3期10卷 1370-1374页

作者：黄文伟万霞张强孙龙林北京交通大学机械与电子控制工程学院北京100044 深圳职业技术学院汽车与交通学院深圳518055

以装配SCR系统的国Ⅳ城市公交车为研究对象,选取典型的深圳城市公交车运行路线,在车辆实际道路状态下,测试了国Ⅳ城市公交车颗粒物的排放特性。实验结果表明：装配SCR系统的国Ⅳ城市公交车排放的颗粒物（PM）在数量上主要是细颗粒物PM1... 详细信息

以装配SCR系统的国Ⅳ城市公交车为研究对象,选取典型的深圳城市公交车运行路线,在车辆实际道路状态下,测试了国Ⅳ城市公交车颗粒物的排放特性。实验结果表明：装配SCR系统的国Ⅳ城市公交车排放的颗粒物（PM）在数量上主要是细颗粒物PM1,且细颗粒物PM1主要来源于车辆行驶在设置交通信号灯较多的城市中心路段上,在颗粒物质量排放分布上主要集中在数量较少的PM1-PM10的较大可吸入颗粒物,且与车辆行驶路况相关性不大;同时,在运行过程中排放颗粒物（PM）的数量浓度和质量浓度的相关性很小。

关键词： SCR 公交车颗粒物排放道路工况