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牛泡沫病毒BBet抑制BTas反式激活功能负调控病毒复制

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南开大学学报（自然科学版） 2024年第1期57卷 45-49页

作者：崔金刚拜晓勃张其程邵鹏谈娟乔文涛南开大学生命科学学院分子微生物与技术教育部重点实验室天津300071

泡沫病毒(foamy virus,FV)属于反转录病毒科中的泡沫病毒亚科、泡沫病毒属.其基因组除编码结构蛋白Gag、Pol及Env外,同时还编码2个非结构蛋白Tas和***是泡沫病毒的正调控蛋白,通过结合在病毒启动子上游的DNA调控元件上正调控病毒基因表... 详细信息

泡沫病毒(foamy virus,FV)属于反转录病毒科中的泡沫病毒亚科、泡沫病毒属.其基因组除编码结构蛋白Gag、Pol及Env外,同时还编码2个非结构蛋白Tas和***是泡沫病毒的正调控蛋白,通过结合在病毒启动子上游的DNA调控元件上正调控病毒基因表达.Bet对病毒复制具有一定的负调控作用,但作用机制尚无报道.利用实验室筛选得到的牛泡沫病毒(bovine fomay virus,BFV)3026可释放株(BFV-Z1),采用不同感染复数(0.01及0.1)感染BFV Bet(BBet)稳定表达细胞系及对照细胞系,分析传代后病毒滴度,确证BBet抑制BFV复制;分析BBet对病毒启动子活性的影响,发现Bet对BFV的2个启动子LTR和IP本底活性影响较小,但BBet可抑制BFV Tas(BTas)对BFV的LTR和IP启动子的反式激活.进一步机制分析发现BBet未显著影响BTas的细胞定位,也不能直接结合启动子上游BTas相关应答序列,推测BBet可能通过抑制BTas结合启动子应答元件之后的分子事件,如募集转录复合物等过程负调控病毒复制.

关键词：牛泡沫病毒 BBet BTas

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复杂场景下集成协作式木结构智能建造技术

粉煤灰综合利用 2024年第2期38卷 140-147页

作者：张隆谢佳硕乔文涛孟丽军石家庄铁道大学河北石家庄050043 河北比智智能科技有限公司河北石家庄050000 道路与铁道工程安全保障省部共建教育部重点实验室(石家庄铁道大学) 河北石家庄050043

为提高木结构建造技术数字化水平,将已有工艺与机械臂运动系统相结合,形成数字化建造工具系统。以切割、开榫、铣削及开孔四种工艺为例,对木结构数字建造技术进行剖析与设计,辅以建造装备系统、控制系统形成高度集成化的加工系统,借助... 详细信息

为提高木结构建造技术数字化水平,将已有工艺与机械臂运动系统相结合,形成数字化建造工具系统。以切割、开榫、铣削及开孔四种工艺为例,对木结构数字建造技术进行剖析与设计,辅以建造装备系统、控制系统形成高度集成化的加工系统,借助可视化编程技术手段对工具系统运动轨迹、几何数据分析、自动适配工具及路径自动规划进行标准程序开发,选取斗拱结构进行建造实践。结果表明:所提出的加工系统可精确、高效完成非标准化、定制化异形结构建造任务,开发的标准程序实现了建构逻辑集成化应用,输入构件模型可自动生成机械臂可识别路径,通过实际建造验证了加工系统和标准程序的可行性及适用性。

关键词：数字建造古建筑榫卯节点机械臂参数化设计

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轻钢密肋墙体竖向极限承载力分析及试验研究

新型建筑材料 2024年第1期51卷 14-20页

作者：柴虹宇巴盼锋乔文涛王骞天津城建大学天津300384 石家庄铁道大学河北石家庄050043

采用有限元软件ABAQUS对不同立柱截面尺寸、立柱间距、墙体高度的轻钢密肋墙体进行有限元数值模拟,研究不同因素对轻钢密肋墙体竖向极限承载力的影响。结合分析结果,设计了4片密肋墙体并进行静载荷试验。结果表明:轻钢密肋墙体数值模拟... 详细信息

采用有限元软件ABAQUS对不同立柱截面尺寸、立柱间距、墙体高度的轻钢密肋墙体进行有限元数值模拟,研究不同因素对轻钢密肋墙体竖向极限承载力的影响。结合分析结果,设计了4片密肋墙体并进行静载荷试验。结果表明:轻钢密肋墙体数值模拟与试验的破坏情况较为一致,并且其竖向极限承载力的模拟值、试验值吻合度较好。依据GB 50017—2017《钢结构设计标准》中轻钢密肋墙体竖向极限承载力计算方法,将计算所得公式值与有限元模拟值、试验值进行对比,最大偏离度0.109,三者的比值均在0.95~1.11,极限承载力公式计算结果吻合较好。

关键词：轻钢密肋墙体极限承载力静载荷试验参数分析数值模拟

Crystal structures of catalytic core domain of BIV integrase: implications for the interaction between integrase and target DNA

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Protein & Cell 2010年第4期1卷 363-370页

作者： Xue Yao Shasha Fang wentao qiao Yunqi Geng Yuequan Shen Tianjin Key Laboratory of Protein Science College of Life SciencesNankai University94 Weijin RoadTianjin 300071China Key Laboratory of Molecular Microbiology and Biotechnology(Ministry of Education) the College of Life ScienceNankai University94 Weijin RoadTianjin 300071China

Integrase plays a critical role in the recombination of viral DNA into the host ***,over the past decade,it has been a hot target of drug design in the fight against type 1 human immunodeficiency virus(HIV-1).Bovine immunodeficiency virus(BIV)integrase has the same function as HIV-1 *** have determined crystal structures of the BIV integrase catalytic core domain(CCD)in two different crystal forms at a resolution of 2.45Åand 2.2Å,*** crystal form I,BIV integrase CCD forms a back-to-back dimer,in which the two active sites are on opposite *** has also been seen in many of the CCD structures of HIV-1 integrase that were determined ***,in crystal form II,BIV integrase CCD forms a novel face-toface dimer in which the two active sites are close to each ***,the distance separating the two active sites is approximately 20Å,a distance that perfectly matches a 5-base pair *** on these data,we propose a model for the interaction of integrase with its target DNA,which is also supported by many published biochemical *** results provide important clues for designing new inhibitors against HIV-1.

关键词： bovine immunodeficiency virus integrase catalytic core domain crystal structure dimerization

HIV-1 Vpr protein activates the NF-κB pathway to promote G2/M cell cycle arrest

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Virologica Sinica 2015年第6期30卷 441-448页

作者： Zhibin Liang Ruikang Liu Yongquan Lin Chen Liang Juan Tan wentao qiao Key Laboratory of Molecular Microbiology and Biotechnology (Ministry of Education) and Key Laboratory of Microbial Functional Genomics (Tianjin) College of Life Sciences Nankai University Laboratory of Viral Diseases NIAID National Institutes of Health Lady Davis Institute Jewish General Hospital Departments of Medicine Mc Gill University Department of Microbiology and Immunology Mc Gill University

Viral protein R(Vpr) plays an important role in the replication and pathogenesis of Human immunodeficiency virus type 1(HIV-1). Some of the various functions attributed to Vpr, including the induction of G2/M cell cycle arrest, activating the NF-κB pathway, and promoting viral reverse transcription, might be interrelated. To test this hypothesis, a panel of Vpr mutants were investigated for their ability to induce G2/M arrest and to activate the NF-κB pathway. The results showed that the Vpr mutants that failed to activate NF-κB also lost the activity to induce G2/M arrest, which suggests that inducing G2/M arrest via Vpr depends at least partially on the activation of NF-κB. This latter possibility is supported by data showing that knocking down the key factors in the NF-κB pathway – p65, Rel B, IKKα, or IKKβ– partially rescued the G2/M arrest induced by *** results suggest that the NF-κB pathway is probably involved in Vpr-induced G2/M cell cycle arrest.

关键词： Human immunodeficiency virus type 1(HIV-1) viral protein R(Vpr) NF-κB G2/M arrest

Lysine residues K66, K109, and K110 in the bovine foamy virus transactivator protein are required for transactivation and viral replication

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Virologica Sinica 2016年第2期31卷 142-149页

作者： Suzhen Zhang Xiaoxu Cui Jing Li Zhibin Liang wentao qiao Juan Tan Key Laboratory of Molecular Microbiology and Technology Ministry of Education College of Life SciencesNankai University Central Lab Tianjin 4th Center Hospital

Bovine foamy virus(BFV) is a complex retrovirus that infects cattle. Like all retroviruses, BFV encodes a transactivator Tas protein(BTas) that increases gene transcription from viral *** encodes two promoters that can interact with BTas, a conserved promoter in the 5' long terminal repeat(LTR) and a unique internal promoter(IP). Our previous study showed that BTas is acetylated by p300 at residues K66, K109, and K110, which markedly enhanced the ability of BTas to bind to DNA. However, whether these residues are important for BFV replication was not determined. Therefore, in this study we provide direct evidence that BTas is required for BFV replication and demonstrate that residues K66, K109, and K110 are critical for BTas function and BFV replication. Full-length infectious clones were generated, which were BTas deficient or contained lysine to arginine(K→R) mutations at position 66, 109, and/or 110. In vivo data indicated that K→R mutations at positions 66, 109, and 110 in BTas impaired transactivation of both the LTR and IP promoters. In addition, the K→R mutations in full-length infectious clones reduced expression of viral proteins, and the triple mutant and BTas deletion completely abrogated viral replication. Taken together, these results indicate that lysine residues at positions 66, 109, and 110 in the BTas protein are crucial for BFV replication and suggest a potential role for BTas acetylation in regulating the viral life cycle.

关键词：转录激活蛋白牛泡沫病毒病毒复制赖氨酸残基内部启动子感染性克隆长末端重复序列

MicroRNAs and their role in viral infection

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Frontiers in Biology 2007年第1期2卷 15-20页

作者： MU Junjie YAO Xue CHEN Qimin GENG Yunqi qiao wentao College of Life Science and Tianjin State Laboratory of Microbial Functional Genomics Nankai UniversityTianjin 300071China

Recently,a class of about 22 nucleotides(nt)small RNA has been discovered in many eukaryotes,termed microRNAs(miRNAs),which have a variety of *** recent findings have demonstrated that viruses can also encode their own ***,other findings reveal a relationship between host miRNA and viral *** findings suggest a tight relationship between host and viral infection via miRNA *** article introduces the miRNAs encoded by viruses and reviews the advances of the interaction of the mammalian host miRNAs and viral infection.

关键词： microRNA(miRNA) viral infection small RNA

cis-acting element located in the bovine foamy virus internal promoter possesses the properties of a transcrip-tional enhancer

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Chinese Science Bulletin 2002年第13期47卷 1108-1112页

作者： wentao qiao Chunguang Guo Shuhui Wang Jinzhong Wang Qimin Chen Yunqi Geng Nankai Univ Coll Life Sci Tianjin 300071 Peoples R China

Bovine foamy virus encodes a transcriptional transactivitor, Tas or Borf-1, which governs the level of viral transcripts initiated by both the promoter in the long terminal repeat (LTR) and the internal promoter (IP) located in the env gene through their cis-acting targets. We have identified and characterized a 72 bp TBS (Borf-1) responsive element located in BFV3026, internal promoter (TREIP) by deletion mutant and transient expression assay. This cis-acting target element in the internal promoter has the properties of a transcriptional enhancer which functions independently of its orientation, position and also in heterologous promoters (BFV LTR and bovine immunodeficiency virus, BIV LTR). Alignments reveal that there are positional similarity and sequence homology among BFV TREIP, SFV-1 TREIP proximal element and SFV-3 TREIPH, which suggests that this kind of cis-acting elements possesses some common functional character.

关键词： bovine foamy virus (BFV) internal promoter (IP) Tas responsive element (TRE) enhancer.

A visible-light-assisted Pd/TiO_(2)gas sensor with carbon nanotubes electrodes for trace formaldehyde detection

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Rare Metals 2024年第1期43卷 257-266页

作者： Can Liu qiao-qiao Zou Bin Liu Yong Zhang School of Physics and Optoelectronics Xiangtan UniversityXiangtan 411105China Hunan Institute of Advanced Sensing and Information Technology Xiangtan UniversityXiangtan 411105China School of Materials Science and Engineering Xiangtan UniversityXiangtan 411105China

Owing to the ppb-level detection standard toward the toxic and harmful gas,the detection of trace gases has become an important subject in the field of indoor environment ***,the traditional resistive gas sensors hardly meet the requirement due to the weak signal generated by trace gas molecules that are difficult to ***,a visible-light-assisted Pd/TiO_(2)gas sensor is proposed to endow the effective detection of trace formaldehyde(HCHO)gas without heating *** from the enhanced photocatalytic properties of TiO_(2)by Pd decoration,the visible-light-assisted Pd/TiO_(2)gas sensor can detect the HCHO gas as low as80×10^(–9)at room *** successful preparation of nanoscale TiO_(2)sensing layer is facilitated by the ultrathin carbon nanotube interdigital electrode in the gas sensor,which avoids the discontinuity of the sensing layer caused by the excessive thickness of the traditional metal *** addition,the whole preparation process of the Pd/TiO_(2)gas sensor with carbon nanotube electrodes is compatible with mainstream CMOS fabrication technology,which is expected to realize the batch fabrication and micro-integrated application of gas *** is expected that our work can provide a new strategy for the batch preparation of high-performance trace HCHO gas sensors and their future applications in portable electronic devices such as smartphones.

关键词： Visible-light-assisted gas sensor Pd/TiO_(2) Trace HCHO detection Carbon nanotubes electrodes