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Comparative analysis of metabolite changes in two contrasting rice genotypes in response to low-nitrogen stress

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The Crop Journal 2018年第5期6卷 464-474页

作者： Xiuqin Zhao wensheng wang Ziyan Xie Yongming Gao Chunchao wang Muhammed Mahbubur Rashid Mohammad Rafiqul Islam Binying Fu Zhikang Li Institute of Crop Sciences National Key Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural Sciences Shenzhen Institute for Innovative Breeding Chinese Academy of Agricultural Sciences

Identification of metabolites responsible for tolerance to low nitrogen availability(low-N)will aid in the genetic improvement of rice yield under nitrogen *** this study,a backcross introgression line(G9)and its recurrent parent Shuhui 527(SH527),which show differential responses to low-N stress,were used to identify metabolites associated with lowN tolerance in *** in metabolite contents in the leaves of G9 and SH527 at three growth stages under low-N stress were assessed by gas chromatography–mass *** metabolites,including amino acids and derivatives,were highly enriched in G9 compared with SH527 under the control condition,suggesting that the two genotypes had basal metabolite ***-N stress induced genotype-specific as well as growth stagedependent metabolite *** induced specifically in G9 that were involved in glycolysis and tricarboxylic acid metabolism were enriched at the tillering and grain filling stages,and metabolites involved in nitrogen and proline metabolism were enriched at the booting *** of pyroglutamate,glutamate,2-oxoglutarate,sorbose,glycerate-2-P,and phosphoenolpyruvic acid in G9 suggests that these metabolites could be involved in low-N stress *** results presented here provide valuable information for further elucidation of the molecular mechanisms of low-N tolerance in crops.

关键词： Rice Low-nitrogen stress Metabolite Nitrogen metabolism

Finite Element Model Updating of Complicated Beam-Type Structures Based on Reduced Super Beam Model

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Journal of Harbin Institute of Technology(New Series) 2019年第3期26卷 68-78页

作者： wensheng wang Haojie Wei Zhonghua Hou Qun Mei Yifan Li Department of Engineering Mechanics Henan University of Science and Technology

A finite element model updating technique for complicated beam-type structures is presented in this ***, a complicated beam-type structure is reduced to a reduced super beam model with a much smaller degree of freedom by using the reduced super beam method, which is based on the classic plane cross-section assumption and displacement interpolation function of beam *** based on the reduced super beam, the analysis of eigensolutions and eigensensitivities from the reduced eigenequation are processed for model updating, which will greatly reduce the computational effort when compared to the traditional model updating methods performed on the global *** techniques are adopted for updating the difference of modal dynamic properties, resulting in optimal values of the structural ***, a complicated stiffened cylindrical shell model and a practical missile structure, served as the illustrative examples, are employed for model updating application, which demonstrate that the reduced super beam-based method is both effective and highly efficient.

关键词： reduced super beam method model updating complicated beam-type structures eigensolution eigensensitivity

Parallels and Connections： Consumption, Environment, and State Formation at Both Ends of Eurasia, 1500-1900

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World History Studies 2015年第1期2卷 100-120页

作者： wang wensheng Department of History University of Hawaii Manoa

The general narration of world history after 1500 is divided into two successivestages： the early modern and modern periods. Demarcated roughly by 1800, they areusually portrayed in subtly different ways and with different emphases. Most studieson the early modern era tend to highlight parallel developments in various parts ofthe world, each of which is assumed to have its own motor of change leading to thecommon fate of modernity. The modern period, by contrast, is often portrayed as amuch more integrated system based on heightened levels of international connectionand exchange. This analytical distinction between parallel and integrating processesrepresents the two major approaches of world history： comparison and *** did this distinction play out in the actual development of the early modern andmodern world history？

关键词：消费结构环境保护历史研究

A Novel Integrated Method for Large-Scale Detection, Identification, and Quantification of Widely Targeted Metabolites： Application in the Study of Rice Metabolomics

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Molecular Plant 2013年第6期6卷 1769-1780页

作者： Wei Chen Liang Gong Zilong GUO wensheng wang Hongyan Zhang Xianqing Liu SibinYu Lizhong Xiong Jie Luo National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University Wuhan430070 China Key Laboratory of Horticultural Plant Biology (Ministry of Education) Huazhong Agricultural University Wuhan 430070 China College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 China

Liquid chromatography-mass spectrometry （LC-MS）-based metabolomics has been facilitated by the con- struction of MSz spectral tag （MS2T） library from the total scan ESI MS/MS data, and the development of widely targeted metabolomics method using MS/MS data gathered from authentic standards. In this report, a novel strategy called step- wise multiple ion monitoring-enhanced product ions （stepwise MIM-EPI） was developed to construct the MS2T library, in which stepwise MIM was used as survey scans to trigger the acquisition of EPI. A total number of 698 （almost） non- redundant metabolites with MS2 spectra were obtained, of which 135 metabolites were identified/annotated. Integrating the data gathered from our MS2T library and other available multiple reaction monitoring （MRM） information, a widely targeted metabolomics method was developed to quantify 277 metabolites, including some phytohormones. Evaluation of the dehydration responses and natural variations of these metabolites in rice leaf not only suggested the coordinated regulation of abscisic acid （ABA） with metabolites such as serotonin derivative（s）, polyamine conjugates under drought stress, but also revealed some C-glycosylated flavones as the potential markers for the discrimination of indica and japonica rice subspecies. The new MS2T library construction and widely targeted metabolomics strategy could be used as a tool for rice functional genomics.

关键词： MS2T library widely targeted metabolic profiling flavonoid rice.

Expanding the Scope of CRISPR/Cpfl-Mediatec Senome Editing in Rice

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Molecular Plant 2018年第7期11卷 995-998页

作者： Shaoya Li Xin Zhang wensheng wang Xiuping Guo Zhichao wu Wenming Du Yunde Zhao Lanqin Xia Institute of Crop Sciences (ICS) Chinese Academy of Agricultural Sciences (CAAS) Beijing 100081 China National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan) Huazhong Agricultural University Wuhan 430070 China Section of Ceil and Developmental Biology University of California San Diego La Jolla CA 92093-0116 USA These authors contributed equally to this article.

Dear Editor The CRISPR/Cas gene editing system offers great potential for functional genomics in plants and crop improvement. The spec- ificity of Cas-directed DNA cleavage is strictly determined by a chimeric single guide RNA （sgRNA） and a short protospacer adjacent motif （PAM） in the genome （Cong et al., 2013; Zetsche et al., 2015）. The widely used SpCas9 and its variants have been shown to recognize PAM sequences in the canonical form NGG and non-canonical NGA, NAG, or NGCG in plants （Miao et al., 2013; Ma et al., 2015; Hu et al., 2016）. CRISPR/Cpfl, a new class 2 CRISPR/Cas system, was recently exploited as an alternative tool for genome editing in various organisms, including plants （Zetsche et al., 2015; Kim et al., 2017; Tang et at., 2017; wang et al., 2017; Xu et al., 2017）. Cpfl utilizes a thymidine-rich PAM site, TTTN, and is guided by a single CRISPR RNA （crRNA）（Zetsche et al., 2015）.

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Dominant early heading without yield drag in a sister-line BC breeding progeny DEH_229 is controlled by multiple genetic factors with maineffect loci

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The Crop Journal 2021年第2期9卷 400-411页

作者： Muhiuddin Faruquee Qiang Zhang Lubiao Zhang Linyun Xu wensheng wang Jiansan Chen Jianlong Xu Zhikang Li Binying Fu Tianqing Zheng Institute of Crop Sciences/National Key Facility for Crop Gene Resources and Genetic Improvement Chinese Academy of Agricultural SciencesBeijing 100081China Shenzhen Institute of Breeding&Innovation Chinese Academy of Agricultural SciencesShenzhen 518120GuangdongChina Institute of Rice Research Guangdong Academy of Agricultural SciencesGuangzhou 510640GuangdongChina

Dominant early heading(DEH)in rice(Oryza sativa L.)is of interest in both breeding and *** genetic mechanisms underlying DEH have remained largely *** have developed a near-isogenic DEH line without yield drag named DEH_229 by sister-line backcross(BC)breeding with MH63,a restorer,as the genetic *** conducted a pilot genetic investigation under both short-day(SD)and long-day(LD)*** DEH line harbored only 1.06%variation in the genome sequence relative to *** variants were distributed throughout the *** QTL mapping by sequencing(QTL-seq)on an F_(2) population derived from a cross of MH63×DEH_229,57 loci were detected under the SD *** mapping employing a genome-wide association study with accessions from the 3000 rice genome sequencing project(3K-RG),reduced the number of QTL by 43.9%.Using Rice Functional Genomics&Breeding(RFGB)database,the number of SNP cluster regions within the QTL regions reduced by 27.3%.Further comparison of the genome variation between DEH_229 and MH63 in addition to gene annotation information revealed a new DEH allele of DTH3 with multiple variable sites as a possible major factor underlying the early-heading phenotype of DEH_*** InDel marker,ZMEH_1,was designed based on the variation between DEH_229 and MH63 within this *** accounted for 86.0%of heading date variation under both SD and LD conditions in 109 randomly chosen progeny derived from extreme lines of the MH63×DEH_229 *** study reveals the genetic complexity of DEH in the near-isogenic line and may provide useful material and marker information for plant molecular breeding.

关键词： Dominant early heading Yield drag Sister-line BC breeding Multiple genetic factors Cryptic genetic variation(CGV) Rice(Oryza sativa L.)

Application of Ultimate Oncoplastic Surgery in Breast-Conserving Surgery

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Chinese Journal of Plastic and Reconstructive Surgery 2020年第3期2卷 137-141页

作者： Zhiqiang MA Mengyuan CHEN Haibin WU Jianxin HE Ruonan wang Wei GUO wensheng wang Department of Breast and Thyroid Plastic Surgery First Affiliated Hospital of Henan UniversityKaifengHenan 475000China

Objective To investigate the application of oncoplastic surgery in breast-conserving *** We retrospectively analyzed the clinical data of 103 breast cancer patients who underwent breast-conserving surgery in the First Affiliated Hospital of Henan *** the patients were female whose tumor volume-to-breast volume ratio was greater than 20%.Fifty-two patients were treated with oncoplastic breast-conversing surgery(observation group),and 51 patients were treated with traditional breast-conserving surgery(control group).The volume of resected tissue,subjective satisfaction with breast shape,objective score of breast shape,and follow-up were compared between the two *** In the observation group,the weight of resected breast tissue was 64.2–172.1 g,with a median of 98.7 *** the control group,the weight of resected breast tissue was 67.5–175.7 g,with a median of 102.3 *** 12 months of follow-up,the subjective satisfaction rate and objective score of breast shape in the observation group were significantly better than those in the traditional breast-conserving surgery group(P0.05).Conclusion Oncoplastic breast-conserving surgery leads to better cosmetic results and a more satisfactory clinical results.

关键词： Breast cancer Oncoplastic surgery Breast-conserving surgery

Working for Harmonious Community——Government role in promoting harmony in community

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The Journal of Human Rights 2010年第5期9卷 28-29页

作者： wang XIAOBING & wang wensheng

The Wannianchang Neighborhood Community in the Chenghua Distric.t, Chengdu City in Sichuan Province,covers an area of about 5.2 square kilometers and has a population of about 58,000. The area is noted for the largest number of factories, floating population and low-income groups. The office of the community, which is the grassroots governmental organ, has done a great deal of solid work to promote social stability and harmony.

关键词： Working for Harmonious Community Government role in promoting harmony in community

Performance Analysis of a Coupled System based on Organic Rankine Cycle and Double Effect Absorption Refrigeration for Waste Heat Recovery in Data Center

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Journal of Thermal Science 2025年第1期34卷 188-205页

作者： LI Peng XU Jiaqi wang Binbin LIU Jianyang ZHAO wensheng HAN Zhonghe School of Energy Power and Mechanical Engineering North China Electric Power University Hebei Key Laboratory of Low Carbon and High Efficiency Power Generation Technology North China Electric Power University

During data center operation,it generates a significant volume of low-grade waste *** recover waste heat,a coupled system including solar collector,double effect absorption refrigeration and organic Rankine cycle is *** system performance is analyzed in *** the organic Rankine cycle,five organic working fluids(R245fa,R245ca,R123,R11,and R113) are selected.R245fa,R113 and R245ca obtain the maximum net power output,thermal efficiency and exergy efficiency,*** the double effect absorption refrigeration system,the evaporation temperature,condensation temperature,and generation pressure affect the COP and exergy *** the generator pressure is unchanged,the COP increases with increasing evaporation temperature and decreasing condensation *** the COP reaches 1.3,the COP slightly decreases as the evaporation temperature or condensation temperature ***,the exergy efficiency of refrigeration systems exhibits the same trend as the COP,and the exergy efficiency maximum value appears at approximately 0.32.A new performance indicator,rPUE,was defined to evaluate the data center power utilization *** flow distribution ratio and heat source temperature were optimized with multi-objective *** the mass flow distribution rate is 0.6 and the heat source temperature is 441.5 K,rPUE and the total unit production costs of the system obtain the optimal solution.

关键词： Exergy