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An electrochemical biosensor based on DNA“nano-bridge”for amplified detection of exosomal microRNAs

Chinese Chemical Letters 2021年第11期32卷 3474-3478页

作者： Jing Zhang Meifeng Hou Guanyu Chen Huifang Mao Wenqian Chen wenshen wang Jinghua Chen College of Life Sciences Fujian Agriculture and Forestry UniversityFuzhou 350002China Department of Pharmaceutical Analysis The School of PharmacyFujian Medical UniversityFuzhou 350108China

Exosomal miRNAs,as potential biomarkers in liquid biopsy for cancer early diagnosis,have aroused widespread ***,an electrochemical biosensor based on DNA“nano-bridge”was designed and applied to detect exosomal microRNA-21(miR-21)derived from breast cancer *** brief,the target miR-21 can specifically open the hairpin probe 1(HP1)labeled on the gold electrode(GE)surface through strand displacement *** the exposed loop region of HP1 can act as an initiator sequence to activate the hybridization chain reaction(HCR)between two kinetically trapped hairpin probes:HP2 immobilized on the GE surface and biotin labeled HP3 in *** HCR leads to the formation of DNA“nano-bridge”tethered to the GE surface with a great deal of“piers”.Upon addition of avidin-modified horseradish peroxidase(HRP),numerous HRP were bound to the formed“nano-bridge”through biotin-avidin interaction to arouse tremendous current *** theory,only a single miR-21 is able to trigger the continuous HCR between HP2 and HP3 until all of the HP2 are *** the proposed biosensor achieved ultrahigh sensitivity toward miR-21 with the detection limit down to 168 amol/L,as well as little cross-hybridization even at the single-base-mismatched *** attempts were also made in the detection of exosomal miR-21 obtained from the MCF-7 of breast cancer cell *** our knowledge,this is the first attempt to built horizontal DNA nano-structure on the electrode surface for exosomal miRNAs *** a word,the high sensitivity,selectivity,low cost make the proposed method hold great potential application for early point-of-care(POC)diagnostics of cancer.

关键词： Electrochemical biosensor DNA"nano-bridge" Hybridization chain reaction Exosomal miRNAs Signal amplification reaction

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M2 macrophage-targeted iron oxide nanoparticles for magnetic resonance image-guided magnetic hyperthermia therapy

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Journal of Materials Science & Technology 2021年第22期81卷 77-87页

作者： wenshen wang Fenfen Li Shibo Li Yi Hu Mengran Xu Yuanyuan Zhang Muhammad Imran Khan Shaozhen wang Min Wu Weiping Ding Bensheng Qiu National Lab for Physical Sciences at the Microscale and the Centers for Biomedical Engineering University of Science and Technology of ChinaHefei230027China The First Affiliated Hospital of Anhui Medical University Hefei230022China

Tumor-associated macrophages(TAMs)play an important role in tumor development and *** particular,M2 TAMs can promote tumor growth by facilitating tumor progression and malignant *** targeted elimination of M2 TAMs to inhibit tumor progression is of great significance for cancer *** oxide nanoparticles based magnetic hyperthermia therapy(MHT)is a classical approach to destroy tumor tissue with deep penetration *** this study,we developed a typical M2 macrophage-targeted peptide(M2pep)functionalized superparamagnetic iron oxide nanoparticle(SPIO)for magnetic resonance imaging(MRI)-guided MHT in an orthotopic breast cancer mouse *** obtained multifunctional SPIO-M2pep with a hydrodynamic diameter of 20 nm showed efficient targeting capability,high transverse relaxivity(149 mM^(-1) s^(-1))and satisfactory magnetic hyperthermia performance in *** vivo studies demonstrated that the SPIO-M2pep based MRI can monitor the distribution of nanoparticles in tumor and indicate the suitable timing for *** M2 macrophage-targeted MHT significantly reduced the tumor volume and the population of pro-tumoral M2 TAMs in *** addition,the SPIO-M2pep based MHT can remodel the tumor immune microenvironment(TIME).The multifunctional SPIO-M2pep with M2 macrophage-targeting ability,high magnetic hyperthermia efficiency,MR imaging capability and effective role in remodeling the TIME hold great potential to improve clinical cancer therapy outcomes.

关键词： M2 macrophages-targeted peptide Iron oxide nanoparticles Magnetic resonance imaging Magnetic hyperthermia therapy Tumor immune microenvironment

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Stable oxygen and carbon isotopes from the planktonic foraminifera Neogloboquadrina pachyderma in the Western Arctic surface sediments:Implications for water mass distribution

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Advances in Polar Science 2011年第4期22卷 205-214页

作者： XIAO wenshen wang Rujian CHENG Xinrong State key laboratory of Marine Geology Tongji University Shanghai 200092 China

Neogloboquadrina pachyderma is the most abundant planktonic foraminifera species found in the modern polar oceans. The δ^18O and δ^13C of N. pachyderma from the Western Arctic Ocean sediments were analyzed to reveal the implications of the proxies to environmental changes. The δ^18O from N. pachyderma in the Chukchi Sea reflect the water mass distribution in this area. Heavier δ^18O values were found along the Anadyr Current （AC） and lighter values in the central and eastern Chukchi Sea..These may reflect the freshwater signal from the Alaska Coastal Current （ACC） and Bering Sea Shelf Water （BSSW）. The light δ^18O signature in the high Arctic basin comes from the freshwater stored in the Arctic surface layer. The δ^18O distribution pattern in the Chukchi Sea is also influenced by the current system. High primary productivity along the AC results in heavy δ^18O. The relatively low primary productivity and the freshwater component from the BSSW and ACC may be the reason for this light δ^13C signal in the central and eastern Chukchi Sea. Our data reveal the importance of well ventilated Pacific Water through the Chukchi Sea into the Arctic Ocean.

关键词： δ^18O δ^13C Neogloboquadrina pachyderma Western Arctic Ocean

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引气剂对水工混凝土性能的影响分析

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广东建材 2024年第6期40卷 42-44页

作者：王文申贵州双源工程建设有限公司

为探究掺入引气剂后水工混凝土的性能差异,文章对掺加引气剂与不掺引气剂的混凝土性能差异进行试验分析。结果表明,混凝土在掺入引气剂后坍落度会出现明显提升,适量掺加引气剂有利于改良混凝土工作性能,同时有助于改善混凝土泌水率;泌... 详细信息

为探究掺入引气剂后水工混凝土的性能差异,文章对掺加引气剂与不掺引气剂的混凝土性能差异进行试验分析。结果表明,混凝土在掺入引气剂后坍落度会出现明显提升,适量掺加引气剂有利于改良混凝土工作性能,同时有助于改善混凝土泌水率;泌水会使混凝土抗渗性能明显下降,引气剂的掺入能够形成大量稳定、独立的微小气泡,强化混凝土抗渗性能;引气剂的掺入能够形成大量相互独立的微小气泡,能够通过调整混凝土孔道结构来实现对混凝土力学性能的改善。

关键词：水工混凝土引气剂泌水抗氯离子渗透抗冻融

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影响冰淇淋质量的因素

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中国乳品工业 1987年第1期 16-21页

作者：王文升东北农学院农业工程系畜产品加工教研室

冰淇淋是一种冷冻乳制品,属固体冷饮食品类,是最佳消暑冷饮制品。近几年来我国冰淇淋的生产发展较快,尤其在盛夏季节,大中小城市都有生产,颇受人们欢迎,可以说,冰淇淋如今已是普遍生产的大众化冷饮食品。因此,保证和提高冰淇淋产品的... 详细信息

冰淇淋是一种冷冻乳制品,属固体冷饮食品类,是最佳消暑冷饮制品。近几年来我国冰淇淋的生产发展较快,尤其在盛夏季节,大中小城市都有生产,颇受人们欢迎,可以说,冰淇淋如今已是普遍生产的大众化冷饮食品。因此,保证和提高冰淇淋产品的质量已成为冰淇淋生产和消费的重要课题。冰淇淋的质量表现在产品的营养与风味、形体和结构、外观、耐融性及卫生指

关键词：影响冰淇淋质量因素

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基于AUTOCAD液压传动原理软件的开发

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浙江工贸职业技术学院学报 2005年第3期5卷 43-47页

作者：王文深浙江工贸职业技术学院浙江温州325003

文章介绍了基于AUTOCAD液压传动原理绘图软件的开发指导思想、内容和开发原理。该系统软件在AutoCAD2000的计算机平台上,利用AutoCAD提供的下拉菜单、图像菜单及工具栏菜单等控制模块菜单程序而开发出来的。软件采用了屏幕图形可视化的... 详细信息

文章介绍了基于AUTOCAD液压传动原理绘图软件的开发指导思想、内容和开发原理。该系统软件在AutoCAD2000的计算机平台上,利用AutoCAD提供的下拉菜单、图像菜单及工具栏菜单等控制模块菜单程序而开发出来的。软件采用了屏幕图形可视化的工具栏按钮和下拉式菜单技术,并可直接调用系统图形库中的标准图形,用户因此可准确快速地绘制出符合国家标准并且大小统一的图形,从而解决了工程设计人员在绘制液压原理图中存在的重复性劳动及图形不规范等问题。相对于其它同类系统来说,该软件在使用时不用输入命令和参数即可获得所需图形,并且具有交互性好、使用方便及扩展性好等特点。

关键词： Autocad2000 二次开发模块菜单图块幻灯片控制菜单

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基于EER模型的零件制造信息模型研究

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机械制造 2007年第1期45卷 44-46页

作者：王文深浙江工贸职业技术学院汽车与机电工程系浙江温州325003

在分析现有特征建模的基础上,提出一种基于加工基元的特征加工方法,并对这种方法进行实例分析。利用这种方法可以解决制造过程中零件的几何、工艺、资源等信息的交换和统一问题。

关键词： EER模型零件信息特征

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X-Y数控精密工作台进给伺服系统摩擦误差的分析

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新技术新工艺 2008年第5期 47-49页

作者：王文深浙江工贸职业技术学院汽机系浙江温州325003

针对一个具体的X-Y数控精密工作台,建立了一种考虑摩擦影响的进给伺服系统数学模型。工作台中的摩擦力是借助二维混合摩擦模型来预测。通过MATLAB仿真模拟与采用球杆仪测量结果比较,分析了工作台在不同工况下做圆运动换象限时误差轨迹... 详细信息

针对一个具体的X-Y数控精密工作台,建立了一种考虑摩擦影响的进给伺服系统数学模型。工作台中的摩擦力是借助二维混合摩擦模型来预测。通过MATLAB仿真模拟与采用球杆仪测量结果比较,分析了工作台在不同工况下做圆运动换象限时误差轨迹出现的尖峰突起现象,进而得到了进给速度及进给半径对摩擦所造成的轮廓误差产生影响的结论。

关键词：伺服系统 X—Y工作台数学模型摩擦误差

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