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Comprehensive Characteristics of MicroRNA Expression Profile Conferring to Rhizoctonia solani in Rice

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Rice science 2020年第2期27卷 101-112页

作者： cao wenlei cao Xinxin ZHAO Jianhua ZHANG Zhaoyang FENG Zhiming OUYANG Shouqiang ZUO Shimin Jiangsu Key Laboratory of Crop Genetics and Physiology/Key Laboratory of Plant Functional Genomics of the Ministry of Education/Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding Agricultural CollegeYangzhou UniversityYangzhou 225009China College of Horticulture and Plant Protection Yangzhou UniversityYangzhou 225009China Joint International Research Laboratory of Agriculture and Agri-Product Safety Yangzhou UniversityYangzhou 225009China Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou 225009China

MicroRNAs(miRNAs)are about 22 nucleotides regulatory non-coding RNAs that play versatile roles in reprogramming plant responses to biotic and abiotic ***,it remains unknown whether miRNAs confer the resistance to necrotrophic fungus Rhizoctonia solani in *** investigate whether miRNAs regulate the resistance to ***,we constructed 12 small RNA libraries from susceptible and resistant rice cultivars treated with water/pathogen at 5 h post inoculation(hpi),10 hpi and 20 hpi,*** taking the advantage of next-generation sequencing,we totally collected 400–450 known miRNAs and 450–620 novel miRNAs from the *** analysis of miRNAs demonstrated different patterns for known and novel miRNAs upon *** ***-four miRNA families were identified to be expressed specifically in rice,and most of them were involved in plant disease resistance.A particular Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis result revealed that a great majority of target genes of regulated miRNAs belonged to the pathway of plant-pathogen ***,miR444b.2,miR531a,mir1861i,novel_miR1956 and novel_miR135 conferred response to *** infection confirmed by Northern *** global understanding of miRNA profiling revealed that the regulation of miRNAs may be implicated in the control of rice immunity to *** of the expression of miRNAs will offer the community with a direction to generate appropriate strategies for controlling rice sheath blight disease.

关键词： rice microRNA sheath blight disease resistance transcriptomics

S Zorb装置长周期生产低硫含量汽油的影响因素及对策

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石油炼制与化工 2014年第2期45卷 74-78页

作者：曹文磊中国石化上海高桥分公司上海200434

比较了中国石化上海高桥分公司S Zorb装置正常生产满足沪Ⅵ排放标准的汽油和生产满足相当于欧Ⅴ排放标准的汽油(硫质量分数不大于10μg/g)时操作参数的调整以及运行情况,总结了装置长周期生产低硫含量汽油的影响因素,并提出了相应的对... 详细信息

比较了中国石化上海高桥分公司S Zorb装置正常生产满足沪Ⅵ排放标准的汽油和生产满足相当于欧Ⅴ排放标准的汽油(硫质量分数不大于10μg/g)时操作参数的调整以及运行情况,总结了装置长周期生产低硫含量汽油的影响因素,并提出了相应的对策。影响长周期生产低硫含量汽油的主要因素有原料性质和进料量的波动、吸附剂循环不畅、阀门设备故障以及反应器过滤器寿命等。在严格控制原料性质并做好设备维护的情况下,S Zorb装置可长周期生产低硫含量汽油。

关键词：吸附脱硫汽油硫含量欧Ⅴ排放标准辛烷值

Integrated Transcriptome and Metabolome Analysis of Oryza sative *** Infection with Rhizoctonia solani,the Causal Agent of Rice Sheath Blight

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Integrated Transcriptome and Metabolome Analysis of Oryza sa...

中国植物病理学会2017年学术年会

作者： cao wenlei Xu Bin Yuan Limin Chen Xijun Pan Xuebiao Zuo Shimin Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou University Testing Center of Yangzhou University

Sheath blight（SB）,caused by necrotrophic fungus Rhizoctonia solani Kiihn（***）,gives rise to severely grain losses in *** this study,an integrated approach of transcriptomics and metabolomics was employed to try to uncover potential mechanism and to mine the candidate genes/pathways regulating rice SB *** is a novel germplasm with stable and high resistance to SB,which has been identified by using greenhouse and field *** analysis by SEM showed that the growth of *** mycelium was apparently suppressed in YSBRl but not in Lemont（susceptible） after 60 HAL *** infection was able to trigger substantialmetabolic reprogramming in rice,particularly in primary metabolismand energy *** and tyrosine,which have been confirmed to involve in plant-pathogen interaction via the biosynthesis of secondary metabolites,were specially increased in YSBRl *** of 332/62（Lemont/YSBR1） and 2606/748（Lemont/YSBRl） DEGsat 10 and 20 HAI were identified,respectively.A total of 247 unique DEGs were identified specifically in YSBR1,which were annotated to involve in several pathways,like amino acid metabolism,secondary metabolic,energy metabolism,photosynthesis,and sugar metabolism *** combining genetic analysis and omics evidence,6 candidate genes localized within the known SBR QTL region（Lemont/YSBR1）,encoding cellulose synthase-like family A,transcription factors TF Ⅱ S,elongin A,EF hand family protein,calmodulin-related calcium sensor protein,ERF transcription factor,and thaumatin,have been identified.

关键词： Rhizoctonia solani Rice Metabolome transcrptome QTLs Candidate genes/pathways

Integrated transcriptome and metabolome analysis of Oryza sative *** infection with Rhizoctonia solani,the causal agent of rice sheath blight

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Integrated transcriptome and metabolome analysis of Oryza sa...

江苏省遗传学会2016年学术年会

作者： wenlei cao Bin Xu Limin Yuan Xijun Chen Xuebiao Pan Shimin Zuo Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou University Testing center of Yangzhou University

Sheath blight（SB）,caused by necrotrophic fungus Rhizoctonia solani Kühn（***）,gives rise to severely grain losses in *** various resistance levels of rice varieties to SB have been reported,the underlying resistance mechanism remains largely unknown due to lack of enough information on candidate genes/*** this study,an integrated approach was employed to try to uncover potential mechanism of rice resistance to *** reveal the molecular mechanism of riceresponse to *** and mine the candidate genes/pathways regulating rice sheath blight（SB） resistance through transcription and metabolite profiling and genetic ***1 is a novel germplasm with stable and high resistance to SB,which has been identified by using greenhouse and field *** analysis by SEM（scanning electronic microscopy） showed that the growth of *** mycelium was apparently suppressed in YSBR1 but not in the susceptible variety Lemont after 60 HAI（hours after inoculation）.*** infection was able to trigger substantial metabolic reprogramming in rice,particularly in primary metabolism and energy *** and tyrosine,which have been confirmed to involve in plant-pathogen interaction via the biosynthesis of secondary metabolites,were specially increased in YSBR1 *** of 332/62（Lemont/YSBR1） and 2606/748（Lemont/YSBR1） DEGs（differential expression genes） at 10 and 20 HAI were identified,*** varieties,24 and 538 DEGs were found with same expression pattern in response to *** 10 and 20 HAI.A total of 247 unique DEGs were identified specifically in YSBR1,which were annotated to involve in several pathways,like amino acid metabolism,secondary metabolic,energy metabolism,photosynthesis,and sugar metabolism *** combining genetic analysis and omics evidence,a few candidate genes/pathways regulating rice SB resistance were identified,among them 6 genes localized within the known SBR QTL region（Lemont/

关键词： Rhizoctonia solani rice metabolome transcrptome QTLs candidate genes/pathways

A Receptor Like Kinase Gene SBRR1 Positively Regulates Resistance to Sheath Blight in Rice

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A Receptor Like Kinase Gene SBRR1 Positively Regulates Resis...

中国植物病理学会2017年学术年会

作者： Feng Zhiming Zhang Yafang Wang Yu Zhang Huimin cao wenlei Chen Zongxiang Pan Xuebiao Zuo Shimin Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops Key Laboratory of Plant Functional Genomics of the Ministry of EducationYangzhou University

Sheath blight（SB）,caused by the necrotrophic fungal pathogen Rhizoctonia solani Kühn,poses a great threat to the rice grain yield as one of the most serious rice *** like kinases play important roles in plant defense responses to ***,receptor like kinases associated with rice SB have not been *** analyzed the differential gene expression profiles of the rice variety YSBR1 with high resistance to SB before and after inoculation with ***,and isolated a rice receptor like kinase gene SBRR1（sheath blight-related RLK gene 1） induced by ***1 is preferentially expressed in leaf sheaths and leaves,which is consistent with the main infective site of ***,implying its role in regulating rice resistance to *** SBRR1 loss-of-function mutant sbrrl is more susceptible to SB compared with its wild ***,knockdown of SBRR1 expression by RNAi reduces the resistance of rice to *** results demonstrated that SBRR1 positively regulates resistance to *** further identified an SBRRl-interaction protein SIP1（SBRR1 interaction protein 1） that encodes an ankyrin repeat protein via yeast two hybrid ***1 is also induced by *** and preferentiaUy expressed in leaf sheaths and *** results enrich the knowledge of the molecular mechanism of resistance to rice SB,which will provide the theories basis for establishing a new strategy to prevent and control this disease.

关键词： Rice sheath blight Receptor like kinase SBRR1 Resistance mechanism

A receptor like kinase gene SBRR1 positively regulates resistance to sheath blight in rice

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A receptor like kinase gene SBRR1 positively regulates resis...

江苏省遗传学会2017年学术研讨会——“技术创新与遗传学发展”

作者： Zhiming Feng Yafang Zhang Yu Wang Huimin Zhang wenlei cao Zongxiang Chen Xuebiao Pan Shimin Zuo Yangzhou University

Sheath blight(SB), caused by the necrotrophic fungal pathogen Rhizoctonia solani Kühn, poses a great threat to the rice grain yield as one of the most serious rice diseases. Receptor like kinases play important roles in plant defense responses to disease. However, receptor like kinases associated with rice SB have not been reported. We analyzed the differential gene expression profiles of the rice variety YSBR1 with high resistance to SB before and after inoculation with R. solani, and isolated a rice receptor like kinase gene SBRR1(sheath blight-related RLK gene 1) induced by R. solani. SBRR1 is preferentially expressed in leaf sheaths and leaves, which is consistent with the main infective site of R. solani, implying its role in regulating rice resistance to SB. The SBRR1 loss-of-function mutant sbrr1 is more susceptible to SB compared with its wild type. Moreover, knockdown of SBRR1 expression by RNAi reduces the resistance of rice to SB. These results demonstrated that SBRR1 positively regulates resistance to SB. We further identified an SBRR1-interaction protein SIP1(SBRR1 interaction protein 1) that encodes an ankyrin repeat protein via yeast two hybrid screening. SIP1 is also induced by R. solani and preferentially expressed in leaf sheaths and leaves. Our results enrich the knowledge of the molecular mechanism of resistance to rice SB, which will provide the theories basis for establishing a new strategy to prevent and control this disease.

关键词： rice sheath blight receptor like kinase SBRR1 resistance mechanism

《美食研究》的特色栏目《饮食文化》建设实践与成效

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编辑学报 2021年第5期33卷 567-570页

作者：王芙蓉赵勇曹文磊扬州大学《美食研究》编辑部江苏扬州225127

"特色栏目"是期刊中富有生命力、感染力的单元。扬州大学专业期刊《美食研究》的《饮食文化》栏目坚持办栏30多年,形成自己稳定的风格和特色。总结探讨特色栏目创办背景、办栏实践和建设成效,探讨其充分利用个刊的内容优势,反哺专业建... 详细信息

"特色栏目"是期刊中富有生命力、感染力的单元。扬州大学专业期刊《美食研究》的《饮食文化》栏目坚持办栏30多年,形成自己稳定的风格和特色。总结探讨特色栏目创办背景、办栏实践和建设成效,探讨其充分利用个刊的内容优势,反哺专业建设、服务地方、弘扬中华优秀传统文化,进而在中外交流过程中以"食"为"媒",起到塑造积极正面国家形象的积极作用。希冀为其他依托民族、传统文化内容资源打造特色栏目的中文期刊提供参考,也为我国期刊内容的特色化传播与期刊国际传播能力提升提供一种思路。

关键词：特色栏目栏目建设国际传播

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连续重整装置节能的途径与方法

石化技术 2002年第4期9卷 220-223页

作者：曹文磊中国石油化工股份有限公司上海高桥分公司炼油厂 200137

连续重整装置是炼油厂的一个用能大户。从标定的数据出发 ,结合装置的用能 ,对连续重整装置的实际用能进行分析 ,针对用能大的问题。

关键词：连续重整装置节能方法炼油能耗

A Sheath Blight Resistance QTL qSB-11LE Encodes a Receptor Like Protein Involved in Rice Early Immune Response to Rhizoctonia solani

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A Sheath Blight Resistance QTL qSB-11LE Encodes a Receptor L...

中国植物病理学会2017年学术年会

作者： Xue Xiang Wang Yu Feng Zhiming Zhang Huimin Shan Wenfeng cao wenlei Zhang Yafang Chen Zongxiang Pan Xuebiao Zuo Shimin Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops Key Laboratory of Plant Functional Genomics of the Ministry of EducationYangzhou University

Sheath blight(SB) is one of the three most serious diseases,which leads to severe grain loss *** resistance to SB disease is a typical of quantitative trait controlled by polygenes or quantitative trait loci(QTLs).Lots of QTLs for SB resistance have been reported while none of them was isolated so far,which significantly hinders the elucidation of resistant ***,we have mapped an SB resistance QTL on chromosome 11 into a 78 kb region,in which the resistant allele(here after called qSB-11) is from Lemont(LE).Here,through a series of experiments like RNA interference,overexpression and complementation tests,we confirmed that a receptor-like protein lack of intracellular domain in the fine mapping region is ***-PCR data showed that qSB-lltranscription was significantly induced by SB pathogen Rhizoctonia solani but not by rice blight pathogen *** qSB-11is highly expressed in leaf sheath at the booting *** localization assay indicated that qSB-11protein localizes in both nucleus and *** the treatments of known pathogen associated molecular patterns(PAMPs),chitin and fig22,we found that knock down of qSB-11significantly affected rice innate immune response to *** known receptor/co-receptor like kinase proteins were found no interaction with qSB-11protein in *** the infection of SB fungus,the synthesis of hormone ethylene(ET) was apparently induced in wild plants,while it was only weakly increased in qSB-11RNAi plants as well as in susceptible near isogenic line(NIL).Spray of ET was able to recover the resistance phenotype of qSB-11 RNAi and NIL ***,we conclude that qSB-11is involved in rice early immune response to SB fungus and regulates ET synthesis upon SB pathogen infection to activate ET-dependent defense response.

关键词： Rice sheath blight QTL qSB-11LE Receptor like protein Early immune response Ethylene