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Regulatory role of electroacupuncture on satellite glial cell activity in the colon and dorsal root ganglion of rats with irritable bowel syndrome

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Journal of Traditional Chinese Medicine 2024年第5期44卷 981-990页

作者： ZHANG Fang YAN Cuina weng zhijun WU Luyi QI Li ZHAO Min XIN Yuhu WU Huangan LIU Huirong Key Laboratory of Acupuncture and Immunological Effects Yueyang Hospital of Integrated Traditional Chinese and Western MedicineShanghai University of Traditional Chinese Medicine Shanghai Research Institute of Acupuncture and Meridian Shanghai University of Traditional Chinese Medicine Cancer Hospital Fudan University

OBJECTIVE: To investigate the role of satellite glial cells in irritable bowel syndrome(IBS) and the effect of electroacupuncture(EA) at the Tianshu(ST25) and Shangjuxu(ST37) combination. METHODS: A model for visceral hypersensitivity in IBS was induced through colorectal distension(CRD) stimulation. Clean-grade male Sprague-Dawley(SD) rats were randomly divided into four groups: a normal group(NG), a model group(MG), an electroacupuncture group(EA), and a glial cell inhibitor group(FCA). Bilateral EA(2/100 Hz, 1 mA, 30 min) was administered at the Tianshu(ST25) and Shangjuxu(ST37) in week 6. Abdominal withdrawal reflex(AWR) scores were used to assess the behavioral response associated with visceral hyperalgesia, while hematoxylin-eosin staining was employed to evaluate pathological changes in the colon. The protein and mRNA levels of glial fibrillary acidic protein(GFAP) in the colon and colon-related dorsal root ganglion(DRG) were analyzed using immun-ofluorescence, immunohistochemistry, Western blotting, real-time polymerase chain reaction. The impact of EA on electrophysiological properties of colon-related DRG neurons was observed through whole-cell patch clamp analysis. RESULTS: EA significantly reduced the visceral pain behavior scores in rats with IBS in response to graded(20, 40, 60, 80 mm Hg) CRD stimulation. Additionally, EA downregulated the protein and mRNA expression levels of GFAP in the colon and colon-related DRG of rats with IBS. EA also regulated the resting membrane potential, rheobase and action potential of colon-related DRG neurons in rats with IBS. CONCLUSIONS: EA can regulate the excitatory properties of colon-related DRG neurons by downregulating the protein and mRNA expression of GFAP in the colon and colon-related DRG, indicating a potential neurobiological mechanism by which EA relieves visceral hypersensitivity in rats with IBS.

关键词： electroacupuncture glial fibrillary acidic proteinSupporting information irritable bowel syndrome satellite glial cell visceral hypersensitivity

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Effect of herb-partitioned moxibustion on dopamine levels and dopamine receptor 1 expression in the colon and central nervous system in rats with Crohn’s disease

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Journal of Traditional Chinese Medicine 2019年第3期39卷 356-363页

作者： Lu Yuan Zheng Handan Lü Tingting Ma Zhe Wu Huangan Xu Manwen weng zhijun Feng Hui Zhang Fang Ding Guanghong Wu Luyi Liu Huirong Department of Mechanics and Engineering Science Fudan University Shanghai 200433China Shanghai Research Institute of Acupuncture and Meridian Shanghai 200030 China Department of Acupuncture and Moxibustion Huashan Hospital Shanghai 200040 China Department of Acupuncture and Moxibustion and Tuina Guanghua Hospital Shanghai 200052 China

OBJECTIVE:To observe the effect of herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints in rats with Crohn's disease,and explore the underlying mechanism from dopamine (DA) and dopamine receptor 1 (D1 R) in the colon,spinal dorsal horn and ***:The rats were randomly divided into the normal,model (CD),herb-partitioned moxibustion (Mox) and mesalazine (Mesa) *** in the colons was scored and observed by hematoxylin and eosin *** and D1R protein expression in the colonic mucosa were detected by *** concentrations of DA and D1R in the spinal dorsal horn and hypothalamus were measured by enzyme-linked immunosorbent assay,and D1R mRNA expression was evaluated by quantitative real-time polymerase chain ***:In the colon,compared with the normal group,DA,D1 R protein expressions and D1 R mRNA expression were significantly higher in the model group,while decreased in the Mox group and the Mesa *** the spinal dorsal horn and hypothalamus,compared with the normal group,the concentrations of DA and D1 R,and the D1R mRNA expressions were significantly higher in the model group,and decreased in the Mox group and the Mesa ***:Herb-partitioned moxibustion at the Tianshu (ST 25) and Qihai (CV 6) acupoints relieved ulceration in CD rats,the underlying mechanism maybe relative with the regulation of DA and D1R in the colon,spinal dorsal horn and hypothalamus by moxibustion.

关键词： Crohn disease Dopamine Receptors,dopamine Neuropeptides Point ST25 (Tianshu) Point CV6 (Qihai) Herb-partitioned moxibustion

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Acupuncture and Moxibustion Inhibited Intestinal Epithelial-Mesenchymal Transition in Patients with Crohn's Disease Induced by TGF-β1/Smad3/Snail Pathway:A Clinical Trial Study

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Chinese Journal of Integrative Medicine 2022年第9期28卷 823-832页

作者： GUO Sen ZHOU Jing ZHANG Liang BAO Chun-hui ZHAO Ji-meng GAO Yan-ling WU Pin weng zhijun SHI Yin Graduate School Shanghai University of Traditional Chinese MedicineShanghai201203China Department of Acupuncture and Moxibustion Shanghai Shanghai Traditional Chinese Medicine Integrated HospitalShanghai University of Traditional Chinese MedicineShanghai200082China Department of Acupuncture and Moxibustion Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese MedicineShanghai200437China Key Laboratory of Acupuncture and Immunological Effects Shanghai Research Institute of Acupuncture and MeridianShanghai200030China Outpatient Department Shanghai Research Institute of Acupuncture and MeridianShanghai200030China

Objective:To explore whether acupuncture combined with moxibustion could inhibit epithelialmesenchymal transition in Crohn’s disease by affecting the transforming growth factorβ1(TGF-β1)/Smad3/Snail ***:Sixty-three patients with Crohn’s disease were randomly divided into an observation group(31 cases)receiving moxibustion at 43℃ combined with acupuncture,and a control group(32 cases)receiving moxibustion at 37℃combined with sham acupuncture using a random number *** were treated for12 ***’s Disease Activity Index(CDAI)was used to evaluate disease ***-eosin staining and transmission electron microscopy were utilized to observe the morphological and ultrastructural *** was used to detect the expression of transforming growth factorβ1(TGF-β1),TβR1,TβR2,Smad3,Snail,E-cadherin and fibronectin in intestinal mucosal ***:The decrease of the CDAI score,morphological and ultrastructural changes were more significant in observation *** expression levels of TGF-β1,TβR2,Smad3,and Snail in the observation group were significantly lower than those before the treatment(P<0.05 or P<0.01).After treatment,the expression levels of TGF-β1,TβR2,and Snail in the observation group were significantly lower than those in the control group(all P<0.05);compared with the control group,the expression of fibronectin in the observation group was significantly decreased,and the expression of E-cadherin was significantly increased(all P<0.05).Conclusions:Moxibustion at 43℃combined with acupuncture may suppress TGF-β1/Smad3/Snail pathway-mediated epithelial-mesenchymal transition of intestinal epithelial cells in Crohn’s disease patients by inhibiting the expression levels of TGF-β1,TβR2,Smad3,and Snail.(Registration ***-IIR-16007751).

关键词： Crohn’s disease moxibustion acupuncture transforming growth factorβ1/Smad3/Snail pathway intestinal epithelial-mesenchymal transition

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Electroacupuncture diminishes P2X_2 and P2X_3 purinergic receptor expression in dorsal root ganglia of rats with visceral hypersensitivity

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Neural Regeneration Research 2013年第9期8卷 802-808页

作者： zhijun weng Luyi Wu Yuan Lu Lidong Wang Linying Tan Ming Dong Yuhu Xin Shanghai Academy of Traditional Chinese Medicine Fudan University Shanghai Cancer Center

Electroacupuncture at Shangjuxu （ST37） and Tianshu （ST25） can improve visceral hypersensitivity in rats. Colorectal distension was used to establish a rat model of chronic visceral hypersensitivity. Immunohistochemistry was used to detect P2X2 and P2X3 receptor expression in dorsal root ganglia from rats with chronic visceral hypersensitivity. Results demonstrated that abdominal withdrawal reflex scores obviously increased following establishment of the model, indicating visceral hypersensitivity. Simultaneously, P2X2 and P2X3 receptor expression increased in dorsal root ganglia. After bilateral electroacupuncture at Shangjuxu and Tianshu, abdominal withdrawal reflex scores and P2X2 and P2X3 receptor expression decreased in rats with visceral hypersensitivity. These results indicated that electroacupuncture treatment improved visceral hypersensitivity in rats with irritable bowel syndrome by reducing P2X2 and P2X3 receptor expression in dorsal root ganglia.

关键词： neural regeneration acupuncture and moxibustion P2X2 P2X3 visceral hypersensitivity irritablebowel syndrome electroacupuncture P2 purinergic receptors abdominal withdrawal reflex scoresacupuncture and moxibustion peripheral nerve injury grants-supported paper photographscontaining paper neuroregeneration

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Electroacupuncture at He-Mu points reduces P2X_4 receptor expression in visceral hypersensitivity

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Neural Regeneration Research 2013年第22期8卷 2069-2077页

作者： Xinxin Guo Jifei Chen Yuan Lu Luyi Wu zhijun weng Ling Yang Yuhu Xin Xianming Lin Yi Liang Jianqiao Fang Third Affiliated Hospital of Zhejiang Chinese Medical University Department of Orthopedics Zhongshan Hospital of Fudan University Shanghai Institute for Traditional Chinese Medicine Cancer Hospital Fudan University

Electroacupuncture at Shangjuxu （ST37） and Tianshu （ST25） was reported to improve visceral hypersensitivity in rats, Colorectal distension was utilized to generate a rat model of chronic visceral hypersensitivity in irritable bowel syndrome, Results showed that abdominal withdrawal reflex scores noticeably increased after model establishment. Simultaneously, P2X4 receptor immunore- activity significantly increased in the colon and spinal cord. Electroacupuncture and pinaverium bromide therapy both markedly decreased abdominal withdrawal reflex scores in rats with visceral hypersensitivity, and significantly decreased P2X4 receptor immunoreactivity in the colon and spinal cord. These data suggest that electroacupuncture treatment can improve visceral hypersensitivity in rats with irritable bowel syndrome by diminishing P2X4 receptor immunoreactivity in the colon and spinal cord,

关键词： neural regeneration traditional Chineseture irritable bowel syndrome visceralported paper neuroregenerationmedicine combination of He-Mu points electroacupunc-hypersensitivity P2X4 receptor acupuncture grants-sup-

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Mechanism of dorsal root ganglion SERT in electroacupuncture regulation of P2X3 receptor-mediated visceral hypersensitivity in IBS rats

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Journal of Acupuncture and Tuina Science 2024年第6期22卷 435-446页

作者： HUANG Rong CHAI Jing ZHOU Yun QIAO Yu weng zhijun WU Huangan LIU Huirong ZHU Lu MA Jindan ZHU Yi ZHANG Fang Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Shanghai University of Traditional Chinese MedicineShanghai 200437China Shanghai Research Institute of Acupuncture and Meridian Shanghai 200030China

Objective:To investigate the role of serotonin reuptake transporter(SERT)and P2X3 receptor of dorsal root ganglion(DRG)in regulating visceral hypersensitivity of rats with irritable bowel syndrome(IBS)by electroacupuncture(EA).Methods:Male Sprague-Dawley and SERT^(-/-)rats were subjected to preparing IBS visceral hypersensitivity models with 2,4,6-trinitrobenzene sulfonic acid(TNBS)*** weeks post-modeling,interventions including EA,intrathecal injection,and EA plus intrathecal injection were applied,***-eosin staining and abdominal withdrawal reflex(AWR)score were used to confirm the successful establishment of the IBS *** score,whole-cell patch clamp technique,and Western blotting assay were used to evaluate the changes in visceral pain sensitivity,electrophysiological properties of DRG neurons,and the expression of DRG P2X3 receptor and SERT in IBS ***:Compared to the model group,the AWR score in the EA group decreased significantly(P<0.05),the resting membrane potential(P<0.05)and the number of action potentials(P<0.05)of DRG neurons reduced,and the baseline intensity increased(P<0.05);additionally,the expression of P2X3 receptor in DRG decreased(P<0.01),and the SERT expression increased(P<0.05).Compared to the P2X3 receptor agonist group,the SERT protein expression in DRG was higher in the EA *** SERT^(-/-)rats,the P2X3 receptor expression in DRG increased in the EA group compared to the model group(P<0.01).Conclusion:EA modulates the electrophysiological characteristics of intestinal primary sensory neurons by regulating the expression of SERT and P2X3 receptor in DRG of IBS *** modulation may contribute to the mechanism by which EA alleviates peripheral sensitization of visceral pain in IBS rats.

关键词： Acupuncture Therapy Electroacupuncture Irritable Bowel Syndrome Visceral Hypersensitivity Visceral Pain Receptors,Purinergic P2X3 Serotonin Reuptake Transporter Rats

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ICU肺炎克雷伯菌分布及耐药性分析

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实用检验医师杂志 2019年第2期11卷 79-81页

作者：翁志骏上海长征医院实验诊断科

目的了解三级甲等综合医院重症加强治疗病房(ICU)肺炎克雷伯菌的分布及耐药趋势,为临床控制感染及合理选择抗菌药物提供依据。方法收集2016年1月-2018年12月上海长征医院ICU分离出的419株肺炎克雷伯菌,采用纸片扩散法(K-B法)和微量肉汤... 详细信息

目的了解三级甲等综合医院重症加强治疗病房(ICU)肺炎克雷伯菌的分布及耐药趋势,为临床控制感染及合理选择抗菌药物提供依据。方法收集2016年1月-2018年12月上海长征医院ICU分离出的419株肺炎克雷伯菌,采用纸片扩散法(K-B法)和微量肉汤稀释法测定细菌的最低抑菌浓度(MIC),参照2018版美国临床和实验室标准化委员会(CLSI)标准判断结果,采用WHONET 5.6软件统计分析肺炎克雷伯菌的耐药性。结果 3年间本院ICU送检标本中肺炎克雷伯菌年平均检出率为17.79%,主要标本来源于痰液(占67.54%)。肺炎克雷伯菌对亚胺培南和美罗培南的耐药率分别为56.56%和54.18%;对阿米卡星和米诺环素的耐药率较低,分别为38.42%和41.05%。肺炎克雷伯菌产超广谱β-内酰胺酶(ESBLs)检出率为19.09%,耐碳青霉烯菌(CRE)检出率为59.19%。结论上海长征医院ICU肺炎克雷伯菌的耐药率较严重,特别是CRE的检出率较高,建议临床加强细菌耐药性监测,对指导临床合理用药具有重要意义。

关键词：重症加强治疗病房肺炎克雷伯菌耐药性

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LNG外罐施工DOKA模板的特点及应用分析

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上海煤气 2019年第6期 5-9页

作者：翁之君上海燃气市北销售有限公司

DOKA模板在LNG储罐外罐施工应用广泛,具有操作简便、施工迅速、安全可靠、承载力强等优点,取得了良好的施工效果。以五号沟LNG扩建项目为例,介绍了DOKA模板的组成、操作工艺、特点及改良等内容,分析其施工中的优势,为该模板体系在LNG工... 详细信息

DOKA模板在LNG储罐外罐施工应用广泛,具有操作简便、施工迅速、安全可靠、承载力强等优点,取得了良好的施工效果。以五号沟LNG扩建项目为例,介绍了DOKA模板的组成、操作工艺、特点及改良等内容,分析其施工中的优势,为该模板体系在LNG工程中能得到更好的应用提供参考。

关键词： LNG外罐 DOKA模板模板应用分析

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P2X3 in primary afferent neurons mediates the relief of visceral hypersensitivity by electroacupuncture in an irritable bowel syndrome rat model

P2X3 in primary afferent neurons mediates the relief of visc...

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2019中国生理学会学术年会暨张锡钧基金第十五届全国青年优秀生理学学术论文交流会及第十三届全国青年生理学工作者学术会议

作者：张方 Zhe Ma zhijun weng Min Zhao Huangan Wu HANDan Zheng Luyi Wu Yuan Lu Chunhui Bao Yanan Liu Huirong Liu Shanghai Research Institute of Acupuncture and Meridian Shanghai University of Traditional Chinese Medicine Shanghai University of Traditional Chinese Medicine

Objective: To reveal the neurobiological mechanism by which the P2 X3 receptor of colonic primary sensory neurons in the dorsal root ganglia(DRG) of the lumbosacral segment(L6-S2)is involved in the alleviation of visceral hypersensitivity by electroacupuncture(EA) at the HeMu points in an irritable bowel syndrome(IBS) rat ***: The IBS chronic visceral pain model was established in clean-grade male Sprague-Dawley(SD) rats according to the method of AL-chaer et *** the 6 th week, bilateral EA at the He-Mu points(2/100 Hz, 1 mA,30 min) was conducted for *** acupoints included the abdominal Tianshu point(ST25) and the Shangjuxu point(ST37).We conducted behavioral studies, histopathology of the colon, electrophysiology, immunofluorescence histochemistry, and real-time polymerase chain reaction(PCR) assays to observe the role of the P2 X3 receptor in the colon and related DRG in relieving visceral hypersensitivity by ***: 1) EA at the He-Mu points significantly reduced the visceral pain behavior scores of the IBS rats under different levels(20,40, 60, 80 mmHg) of colorectal distention(CRD) stimulation.2) EA downregulated the expression levels of the P2 X3 protein and its messenger RNA(mRNA) in the colon and related DRG of the IBS rats.3) EA regulated the electrical properties of the membranes [the resting membrane potential(RMP), rheobase and action potential(AP)] of colon-associated DRG neurons in the IBS rats with visceral ***: EA can regulate the excitatory properties of DRG neurons by regulating the abnormally elevated P2 X3 receptor protein and mRNA expression levels in the colon and related DRG of IBS rats with visceral *** regulation of P2 X3 may be the important neurobiological mechanism by which EA at the He-Mu points relieves visceral hypersensitivity in IBS rats.

关键词： irritable bowel syndrome visceral hypersensitivity peripheral sensitization P2X3 receptor electroacupuncture

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Th17/Treg细胞在健脾方防治克罗恩大鼠中的作用机制

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世界中医药 2017年第6期12卷 1407-1412,1416页

作者：吴璐一翁志军季光上海市气功研究所上海200030 上海中医药大学上海201203 上海中医药大学附属龙华医院上海200032

目的:观察Th17/Treg细胞平衡在健脾方防治TNBS诱导的克罗恩大鼠中的作用机制;方法:24只清洁级雄性SD大鼠随机分为:1)正常对照组NG(n=8):从造模第2周起双蒸水灌胃,1次/d,灌胃量按1 mL/100 g,共3周;2)TNBS模型组MG(n=8):从第1周开始TNBS灌... 详细信息

目的:观察Th17/Treg细胞平衡在健脾方防治TNBS诱导的克罗恩大鼠中的作用机制;方法:24只清洁级雄性SD大鼠随机分为:1)正常对照组NG(n=8):从造模第2周起双蒸水灌胃,1次/d,灌胃量按1 mL/100 g,共3周;2)TNBS模型组MG(n=8):从第1周开始TNBS灌肠,灌肠剂量(m L)=体重(g)×0.003 m L/g,1次/周,造模持续共4周;3)模型+健脾方防治组JP(n=8):从造模第2周开始,在TNBS灌肠后的第2天新增中药健脾方灌胃治疗,灌胃量按1 m L/100 g,1次/d,灌胃持续3周;采用ELISA、免疫组织化学、real-time PCR等技术观察各组大鼠血液、结肠黏膜中与Th17、Treg分化和功能密切相关的IFN-γ、IL-17、RORγt、FoxP3蛋白与基因的表达差异;结果:健脾方能通过下调Th1细胞释放的IFN-γ,进而来调节Th1/Th2的平衡,同时也能通过下调Th17细胞分泌IL-17、RORγt炎性反应因子的分泌和促进Treg细胞分泌的抗炎因子FoxP3来调节Th17/Treg的平衡,进而达到防治TNBS诱导的大鼠克罗恩病的作用。结论:健脾方能够通过调节Th17/Treg细胞平衡中多个关键细胞因子和转录因子的方式,达到预防和治疗TNBS诱导的大鼠克罗恩病炎性反应程度之目的。

关键词：克罗恩病健脾方 IFN-γ IL-17 RORγt FoxP3

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