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Functionalized TiCu/TiCuN coating promotes osteoporotic fracture healing by upregulating the Wnt/β-catenin pathway

Regenerative Biomaterials 2023年第1期10卷 126-139页

作者： Jia Tan Ling Ren Kai Xie Lei Wang wenbo jiang Yu Guo Yongqiang Hao Department of Orthopaedic Surgery Shanghai Key Laboratory of Orthopaedic ImplantsShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China Clinical and Translational Research Center for 3D Printing Technology Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China Institute of Metal Research Chinese Academy of SciencesShenyang 110000China Musculoskeletal Tumor Center Peking University People’s HospitalBeijing 100044China

Osteoporosis results in decreased bone mass and insufficient osteogenic *** titanium alloy implants have insufficient osteoinductivity and delayed/incomplete fracture union can occur when used to treat osteoporotic *** ions have good osteogenic activity,but their dose-dependent cytotoxicity limits their clinical use for bone *** this study,titanium alloy implants functionalized with a TiCu/TiCuN coating by arc ion plating achieved a controlled release of copper ions in vitro for 28 *** coated alloy was co-cultured with bone marrow mesenchymal stem cells and showed excellent biocompatibility and osteoinductivity in vitro.A further exploration of the underlying mechanism by quantitative real-time polymerase chain reaction and western blotting revealed that the enhancement effects are related to the upregulation of genes and proteins(such as axin2,β-catenin,GSK-3β,p-GSK-3β,LEF1 and TCF1/TCF7)involved in the Wnt/β-catenin *** vivo experiments showed that the TiCu/TiCuN coating significantly promoted osteoporotic fracture healing in a rat femur fracture model,and has good in vivo biocompatibility based on various staining *** study confirmed that TiCu/TiCuN-coated Ti promotes osteoporotic fracture healing associated with the Wnt *** the coating effectively accelerates the healing of osteoporotic fractures and improves bone quality,it has significant clinical application prospects.

关键词： nitride-titanium-copper orthopaedic implant osteogenesis osteointegration osteoporotic fractures

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Generating 3D-cultured organoids for pre-clinical modeling and treatment of degenerative joint disease

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Signal Transduction and Targeted Therapy 2021年第12期6卷 3386-3389页

作者： Ye Sun Qiang Wu Kerong Dai Yongqing You wenbo jiang Department of Orthopaedics The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsuChina Clinical and Translational Research Center for 3D Printing Technology Shanghai Key Laboratory of Orthopaedic ImplantsDepartment of Orthopaedic SurgeryShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghaiChina Department of Nephrology Affiliated Hospital of Nanjing Medical UniversityNorth District of Suzhou Municipal HospitalSuzhouChina

Dear Editor,Human cell-based and personalized in vitro cartilage models are urgently needed for osteoarthritis treatment in pre-clinical regenerative medicine *** self-assemblies and condensations of the appropriate stem cells could initiate the formation of transient tissue structures programmed for specific organogenesis processes.1 This recapitulation of developmental events has previously been demonstrated for the formation of cardiac,epithelial and liver ***,there has been very limited progress in the development of human cartilage organoids for osteoarthritis(OA).2 Here,we describe the fabrication of functional bioengineered cartilage organoid suitable for OA ***,agarose microwell inserts for formation of a high number of synovial mesenchymal stromal cell(SMSC)organoids with homogeneous size distribution were created as previously described by Leijten et al.33D-cultured SMSC organoids were generated and phenotypically analyzed for potential applications in OA modeling and treatment(Fig.1a).

关键词： clinical treatment organo

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Automatic Extraction Method of Weld Weak Defect Features for Ultra-High Voltage Equipment

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Energy Engineering 2023年第4期120卷 985-1000页

作者： Guanghua Zheng Chaolin Luo Mengen Shen Wanzhong Lv wenbo jiang Weibo Yang jiangsu Key Laboratory of Mine Mechanical and Electrical Equipment School of Mechatronic EngineeringChina University of Mining and TechnologyXuzhou22116China Anji Chang Hong Chain Manufacturer Co. Ltd.Anji313300China Pearl RiverWater Resources Research Institute PRHRIGuangzhou510000China Henan Pinggao Electric Co. Ltd.Pinggao Group Co.Ltd.Pingdingshan467001China

To solve the problems of low precision of weak feature extraction,heavy reliance on labor and low efficiency of weak feature extraction in X-ray weld detection image of ultra-high voltage(UHV)equipment key parts,an automatic feature extraction algorithm is ***,the original weld image is denoised while retaining the characteristic information of weak defects by the proposed monostable stochastic resonance ***,binarization is achieved by combining Laplacian edge detection and Otsu threshold ***,the automatic identification of weld defect area is realized based on the sequential traversal of binary *** characteristic analysis dimensions are established for weld defects of UHV key parts,including defect area,perimeter,slenderness ratio,duty cycle,*** experiment using theweld detection image of the actual production site shows that the proposedmethod can effectively extract theweak feature information ofweld defects and further provide reference for decision-making.

关键词： UHV equipment weld nondestructive testing weak feature extraction

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Formability of Printing Ink for Melt Electrowriting

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Journal of Shanghai Jiaotong university(Science) 2021年第3期26卷 411-415页

作者： HAN Yu SUN Binbin jiang wenbo DAI Kerong Department of Orthopaedic Surgery Shanghai Key Laboratory of Orthopaedic ImplantsClinical and Translational Research Center for 3D Printing TechnologyShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai200011China

3D printing,also called additive manufacturing,is being used increasingly in tissue ***,the printing accuracy remains limited,making it difficult to prepare a tissue engineering scaffold with high precision and high *** electrowriting(MEW)technology is based on extrusion printing,in which an extruded material is pulled by the action of an electric field,thereby reducing the fiber diameter and improving the printing ***,MEW technology imposes high requirements on the material properties,and therefore,few printing materials are currently available for use in this *** present study investigates the characteristics and molding conditions of polycaprolactone,a commonly used printing material,as well as other materials such as poly(lactic-co-glycolic acid),poly(ethylene glycol)diacrylate/polyethylene oxide,gelatin methacrylate,and hyaluronic acid methacrylate for MEW applications,and develops new and suitable inks for MEW that will provide more and better choices for constructing a bioactive scaffold in future tissue engineering *** suggest that a printing ink should have low electrical conductivity,suitable viscosity,and high curing speed for realizing successful printing.

关键词： 3D printing melt electrowriting(MEW) conductivity viscosity Taylor cone

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3D Printing Bioink Preparation and Application in Cartilage Tissue Reconstruction in Vitro

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Journal of Shanghai Jiaotong university(Science) 2021年第3期26卷 267-271页

作者： SUN Binbin HAN Yu jiang wenbo DAI Kerong Department of Orthopaedic Surgery Shanghai Key Laboratory of Orthopaedic ImplantsClinical and Translational Research Center for 3D Printing TechnologyShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai200011China

Three-dimensional(3D)bioprinting technology has great potential for application in the treatment of cartilage ***,the preparation of biocompatible and stable bioinks is still a major *** this study,decellularized extracellular matrix(dECM)of soft tissue was used as the basic material to prepare the *** results showed that this novel dECM-derived bioink had good printing performance and comprised a large number of fine *** characterization revealed that the bioink was compatible with the growth of chondrocytes and that the nanofibrous structure greatly promoted cell *** and immunohistochemical analyses showed that the in vitro printed cartilage displayed the presence of characteristic cartilage ***,a new preparation method for dECM-derived bioink with potential application in generation of cartilage was developed in this study.

关键词： bioink cartilage 3D printing tissue engineering biomaterials

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3D Printing Hip Prostheses Offer Accurate Reconstruction,Stable Fixation,and Functional Recovery for Revision Total Hip Arthroplasty with Complex Acetabular Bone Defect

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Engineering 2020年第11期6卷 1285-1290页

作者： Yongqiang Hao Lei Wang wenbo jiang Wen Wu Songtao Ai Lu Shen Shuang Zhao Kerong Dai Department of Orthopedics Shanghai Ninth People's HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200011China Medical 3D Printing Innovation Research Center Shanghai Ninth People's HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200011China Department of Radiology Shanghai Ninth People's HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200011China

Complicated and large acetabular bone defects present the main challenges and difficulty in the revision of total hip arthroplasty(THA).This study aimed to explore the advantages of three-dimensional(3D)printing technology in the reconstruction of such acetabular bone *** retrospectively analyzed the prognosis of four severe bone defects around the acetabulum in three patients who were treated using 3D printing *** of bone defect by conventional methods was difficult in these *** this endeavor,we used radiographic methods,related computer software such as Materialise's interactive medical image control system and Siemens NX software,and actual surgical experience to estimate defect volume,prosthesis stability,and installation accuracy,***,a Harris hip score was obtained to evaluate limb *** was found that bone defects could be adequately reconstructed using a 3D printing prosthesis,and its stability was *** Harris hip score indicated a very good functional recovery in all three *** conclusion,3D printing technology had a good therapeutic effect on both complex and large bone defects in the revision of *** was able to achieve good curative effects in patients with large bone defects.

关键词： 3D printing Hip revision Complex and large acetabular bone defect Accurate reconstruction

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The APETALA2–MYBL2 module represses proanthocyanidin biosynthesis by affecting formation of the MBW complex in seeds of Arabidopsis thaliana

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Plant Communications 2024年第3期5卷 141-155页

作者： wenbo jiang Qinggang Yin Jinyue Liu Xiaojia Su Xiaoyan Han Qian Li Jin Zhang Yongzhen Pang Institute of Animal Science Chinese Academy of Agricultural SciencesBeijing 100193China Key Laboratory of Plant Resources and Beijing Botanical Garden Institute of BotanyChinese Academy of SciencesBeijing 100093China

Proanthocyanidins(PAs)are the second most abundant plant phenolic natural *** biosynthesis is regulated by the well-documented MYB/bHLH/WD40(MBW)complex,but how this complex itself is regu-lated remains ill ***,in situ hybridization and b-glucuronidase staining show that APETALA2(AP2),a well-dened regulator offlower and seed development,is strongly expressed in the seed coat endothelium,where PAs ***2 negatively regulates PA content and expression levels of key PA pathway ***2 activates MYBL2 transcription and interacts with MYBL2,a key suppressor of the PA ***2 exerts its function by directly binding to the AT-rich motifs near the promoter region of *** and biochemical analyses revealed that AP2 forms AP2–MYBL2–TT8/EGL3 com-plexes,disrupting the MBW complex and thereby repressing expression of ANR,TT12,TT19,and *** analyses revealed that AP2 functions upstream of MYBL2,TT2,and TT8 in PA *** work reveals a new role of AP2 as a key regulator of PA biosynthesis in ***,this study sheds new light on the comprehensive regulation network of PA biosynthesis as well as the dual regulatory roles of AP2 in seed development and accumulation of major secondary metabolites in Arabidopsis.

关键词： Arabidopsis complex thereby

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A 3D-Bioprinted dual growth factor-releasing intervertebral disc scaffold induces nucleus pulposus and annulus fibrosus reconstruction

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Bioactive Materials 2021年第1期6卷 179-190页

作者： Binbin Sun Meifei Lian Yu Han Xiumei Mo wenbo jiang Zhiguang Qiao Kerong Dai Department of Orthopaedic Surgery Shanghai Key Laboratory of Orthopaedic ImplantsShanghai Ninth People's HospitalShanghai Jiao Tong University School of MedicineShanghai200080China Clinical and Translational Research Center for 3D Printing Technology Shanghai Ninth People's HospitalShanghai Jiao Tong University School of MedicineShanghai200011China Department of Prosthodontics Shanghai Ninth People's HospitalCollege of StomatologyShanghai Jiao Tong University School of MedicineNational Clinical Research Center for Oral DiseasesShanghai Key Laboratory of Stomatology&Shanghai Research Institute of StomatologyShanghai200011China State Key Lab for Modification of Chemical Fibers&Polymer Materials College of ChemistryChemical Engineering and BiotechnologyDonghua UniversityShanghai201620China Department of Orthopaedic Surgery Renji HospitalSouth CampusShanghai Jiao Tong University School of MedicineShanghai201112China

Regeneration of Intervertebral disc(IVD)is a scientific challenge because of the complex structure and composition of tissue,as well as the difficulty in achieving bionic ***,an anatomically correct IVD scaffold composed of biomaterials,cells,and growth factors were fabricated via three-dimensional(3D)bioprinting *** tissue growth factor(CTGF)and transforming growth factor-β3(TGF-β3)were loaded onto polydopamine nanoparticles,which were mixed with bone marrow mesenchymal stem cells(BMSCs)for regenerating and simulating the structure and function of the nucleus pulposus and annular *** vitro experiments confirmed that CTGF and TGF-β3 could be released from the IVD scaffold in a spatially controlled manner,and induced the corresponding BMSCs to differentiate into nucleus pulposus like cells and annulus fibrosus like ***,the fabricated IVD scaffold was implanted into the dorsum subcutaneous of nude *** reconstructed IVD exhibited a zone-specific matrix that displayed the corresponding histological and immunological phenotypes:primarily type II collagen and glycosaminoglycan in the core zone,and type I collagen in the surrounding *** testing results demonstrated that it exhibited good biomechanical function of the reconstructed *** results presented herein reveal the clinical application potential of the dual growth factors-releasing IVD scaffold fabricated via 3D ***,the evaluation in large mammal animal models needs to be further studied.

关键词： Intervertebral disc(IVD) Regenerative medicine 3D bioprinting Mesenchymal stem cells(MSCs) Growth factor(GF)

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Fabrication of 3D matrix microenvironment by two-photon lithography for mechanobiology study

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Mechanobiology in Medicine 2023年第1期1卷 10-16页

作者： Zuyan Lu wenbo jiang Weixin Zhao Jie Zhao Kerong Dai Shanghai Key Laboratory of Orthopedic Implants Department of Orthopedic SurgeryShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghaiChina Clinical and Translational Research Center for 3D Printing Technology Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghaiChina Wake Forest Institute for Regenerative Medicine Wake Forest University Health SciencesWinston–SalemNCUSA Shanghai Frontiers Science Center of Degeneration and Regeneration in Skeletal System ShanghaiChina

Mechanobiology is the study of how mechanical forces affect biological systems,including cells and *** two-photon lithography(TPL)as a powerful 3D printing technique allows the creation of 3D complex structures at a microscopic *** applying the TPL into the mechanobiology studies,researchers could create precise structures that mimic the mechanical properties of biological system,allowing for the study of mechanobiological processes in a controlled *** implies applications in tissue engineering,drug screening,and fundamental research into the mechanisms of *** this review,we highlight recent advances in TPL for mechanobiology studies,as well as the potential future directions for this promising field.

关键词： Two-photon lithography Mechanobiology 3D printing Microenvironment

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Clinical Observation of 3D Printing Technology in Insoles for Flexible Flatfoot Patients

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Journal of Shanghai Jiaotong university(Science) 2021年第3期26卷 398-403页

作者： WANG Yitian jiang wenbo GAN Yaokai YU Yang DAI Kerong Department of Orthopaedic Surgery Shanghai Jiao Tong University School of MedicineShanghai200011China Shanghai Key Laboratory of Orthopaedic Implants Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai200011China

Flatfoot is defined as the flattening of the medial arch of the foot,and it is classified into flexible flatfoot and rigid flatfoot based on whether the flattening of the medial arch of the foot can be reset when standing on *** insole is the most basic and common treatment,which is relatively cheaper and easier to ***-dimensional(3D)printing,an emerging technology characterized by high machining accuracy and use of various materials,can be utilised in personalised insoles,which have good application *** research on the clinical effects of 3D-printed insoles is still *** this study,64 cases of 3D-printed insoles were clinically *** results showed that 3D-printed insoles had statistically positive effects in treating flatfoot(P=0.00017),and with adjustment and adaptation,their comfort and clinical effect can be *** study provides an empirical reference for further large-scale clinical control research.

关键词： flexible flatfoot orthotic insoles 3D printing

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