The APETALA2–MYBL2 module represses proanthocyanidin biosynthesis by affecting formation of the MBW complex in seeds of Arabidopsis thaliana

作     者：Wenbo Jiang Qinggang Yin Jinyue Liu Xiaojia Su Xiaoyan Han Qian Li Jin Zhang Yongzhen Pang 

作者机构：Institute of Animal ScienceChinese Academy of Agricultural SciencesBeijing 100193China Key Laboratory of Plant Resources and Beijing Botanical GardenInstitute of BotanyChinese Academy of SciencesBeijing 100093China 

出 版 物：《Plant Communications》 (植物通讯（英文）)

年 卷 期：2024年第5卷第3期

页      面：141-155页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by grants from the National Natural Science Foundation of China (31870281 to W.J.) the Agricultural Science and Technology Innovation Program (ASTIP-IAS10) 

主　　题：Arabidopsis complex thereby 

摘      要：Proanthocyanidins(PAs)are the second most abundant plant phenolic natural *** biosynthesis is regulated by the well-documented MYB/bHLH/WD40(MBW)complex,but how this complex itself is regu-lated remains ill ***,in situ hybridization and b-glucuronidase staining show that APETALA2(AP2),a well-dened regulator offlower and seed development,is strongly expressed in the seed coat endothelium,where PAs ***2 negatively regulates PA content and expression levels of key PA pathway ***2 activates MYBL2 transcription and interacts with MYBL2,a key suppressor of the PA ***2 exerts its function by directly binding to the AT-rich motifs near the promoter region of *** and biochemical analyses revealed that AP2 forms AP2–MYBL2–TT8/EGL3 com-plexes,disrupting the MBW complex and thereby repressing expression of ANR,TT12,TT19,and *** analyses revealed that AP2 functions upstream of MYBL2,TT2,and TT8 in PA *** work reveals a new role of AP2 as a key regulator of PA biosynthesis in ***,this study sheds new light on the comprehensive regulation network of PA biosynthesis as well as the dual regulatory roles of AP2 in seed development and accumulation of major secondary metabolites in Arabidopsis.