检索结果-南通市图书馆

在线全文

学校读者我要写书评

暂无评论

老年肌肉减少症与新型炎症标志物的相关性

中国骨质疏松杂志 2024年第1期30卷 50-55页

作者：蒋思琪黄欢欢胡吉丽罗业涛王春妮罗欣黄琪陈梅赵庆华贾雯碧重庆医科大学附属第一医院护理部重庆400016 大溪沟街道社区卫生服务中心护士站重庆400015 陆军军医大学第二附属医院感染控制科重庆400037 重庆医科大学公共卫生学院重庆400016 重庆医科大学附属璧山医院(重庆市璧山区人民医院)护理部重庆402760

目的了解炎症标志物与老年肌肉减少症之间的相关性。方法2023年1~6月在社区卫生服务中心连续招募符合纳入排除标准的老年人,根据亚洲肌少症工作组(AWGS)2019将其分为非肌肉减少症组、肌肉减少症组。分析两组临床资料,采用多因素Logisti... 详细信息

目的了解炎症标志物与老年肌肉减少症之间的相关性。方法2023年1~6月在社区卫生服务中心连续招募符合纳入排除标准的老年人,根据亚洲肌少症工作组(AWGS)2019将其分为非肌肉减少症组、肌肉减少症组。分析两组临床资料,采用多因素Logistic回归筛选老年肌肉减少症的危险因素。采用受试者工作特征(RoC)曲线分析炎性指标对老年肌肉减少症的诊断价值。采用Spearman相关性分析探讨炎性指标与肌肉减少症相关指标的相关性。结果共纳入研究对象377名,肌肉减少症的检出率为17.51%。Logistic多因素回归分析显示,年龄(oR=1.147,95%CI:1.091~1.206)、全身免疫炎症指数(SII)(oR=1.043,95%CI:1.025~1.061)和中性粒细胞/淋巴细胞比率(NLR)(oR=3.236,95%CI:1.984~5.278)、体质量指数(BMI)(oR=1.147,95%CI:1.091~1.206)是老年人发生肌肉减少症的危险因素。RoC曲线显示,SII、NLR、血小板/淋巴细胞比值(PLR)三者联合诊断能力高于3个单项指标,曲线下面积(AUC)为0.818(95%CI:0.764~0.872)。Spearman相关性分析结果显示,SII、PLR与肌肉健康指标呈负相关(P<0.05)。结论老年肌肉减少症与SII、NLR、PLR等新型炎症标志物具有一定相关性,对肌肉减少症的早期筛查和干预有重要意义。

关键词：肌肉减少症炎症全身免疫炎症指数老年人

Introduction of temperature-sensitive helper and donor plasmids into Bac-to-Bac baculovirus expression systems

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2015年第5期30卷 379-385页

作者： Zhihong huang Ao Li Mengjia Pan wenbi Wu Meijin Yuan Kai Yang State Key Laboratory of Biocontrol Sun Yat-sen University

In the baculovirus shuttle vector(bacmid) system, a helper plasmid and a donor plasmid are employed to insert heterologous genes into a cloned baculovirus genome via Tn7 transposition in Escherichia coli. The helper and donor plasmids are usually cotransfected with constructed bacmids into insect cells, which will lead to integration of these plasmids into the viral genome,and hence to the production of defective virions. In this study, to facilitate the preparation of plasmid-free recombinant bacmids, we modified a set of helper and donor plasmids by replacing their replication origins with that of a temperature-sensitive(ts) plasmid, p SIM6. Using the resulting ts helper plasmid p MoN7124 ts and the ts donor plasmid p FB1ts-PH-GFP, a recombinant bacmid,b Ac WT-PG(-), was constructed, and the transposition efficiency was found to be 33.1%. The plasmids were then removed by culturing at 37 °C. For b Ac WT-PG(-), the infectious progeny virus titer and the protein expression level under the control of the polyhedrin promoter were similar to those of a bacmid constructed with unmodified helper and donor plasmids. These ts plasmids will be useful for obtaining plasmid-free bacmids for both heterologous protein production and fundamental studies of baculovirus biology.

关键词： baculovirus Bac-to-Bac baculovirus expression syst

Autographa Californica Multiple Nucleopolyhedrovirus P48(Ac103) Is Required for the Efficient Formation of Virus-Induced Intranuclear Microvesicles

在线全文

学校读者我要写书评

暂无评论

Virologica Sinica 2019年第6期34卷 712-721页

作者： Yan Wang Qingyun Cai Jiannan Chen Zhihong huang wenbi Wu Meijin Yuan Kai Yang State Key Laboratory of Biocontrol Sun Yat-sen University

our previous study has shown that the Autographa californica multiple nucleopolyhedrovirus(AcMNPV) p48(ac103)gene is essential for the nuclear egress of nucleocapsids and the formation of occlusion-derived virions(oDVs). However,the exact role of p48 in the morphogenesis of oDVs remains unknown. In this study, we demonstrated that p48 was required for the efficient formation of intranuclear microvesicles. To further understand its functional role in intranuclear microvesicle formation, we characterized the distribution of the P48 protein, which was found to be associated with the nucleocapsid and envelope fractions of both budded virions and oDVs. In Ac MNPV-infected cells, P48 was predominantly localized to nucleocapsids in the virogenic stroma and the nucleocapsids enveloped in oDVs, with a limited but discernible distribution in the plasma membrane, nuclear envelope, intranuclear microvesicles, and oDV envelope. Furthermore,coimmunoprecipitation assays showed that among the viral proteins required for intranuclear microvesicle formation, P48 associated with Ac93 in the absence of viral infection.

关键词： P48 Baculovirus Intranuclear microvesicle formation Protein association Ac93

PCoS患者血清维生素D与同型半胱氨酸的表达及相关性

在线全文

学校读者我要写书评

暂无评论

现代医药卫生 2024年第19期40卷 3281-3284,3290页

作者：邹文毕廖美艳廖思钦池青黄光秀佛山市三水区人民医院广东佛山528100

目的探讨血清维生素D与同型半胱氨酸(Hcy)在多囊卵巢综合征(PCoS)诊断中的价值及其相关性。方法选取2022年1-12月于该院生殖中心门诊就诊或住院的18~40岁育龄期PCoS患者54例作为PCoS组,同时纳入50例18~40岁健康体检女性作为对照组。检... 详细信息

目的探讨血清维生素D与同型半胱氨酸(Hcy)在多囊卵巢综合征(PCoS)诊断中的价值及其相关性。方法选取2022年1-12月于该院生殖中心门诊就诊或住院的18~40岁育龄期PCoS患者54例作为PCoS组,同时纳入50例18~40岁健康体检女性作为对照组。检测所有受试者血清维生素D与Hcy水平,比较PCoS组和对照组的差异。采用受试者操作特征(RoC)曲线分析单独或联合维生素D和Hcy对PCoS的诊断效能。采用Pearson相关分析探究对照组、PCoS组维生素D水平低于cut-off值(47.16 nmol/L)的人群(低维生素D组)中血清维生素D与Hcy的相关性。结果PCoS组患者的体重[(66.98±11.27)kg]、身体质量指数(BMI)[(26.98±3.79)kg/m^(2)]和血清Hcy水平[(11.10±2.59)μmol/L]显著高于对照组[(61.26±6.66)kg、(24.04±4.28)kg/m^(2)、(9.16±1.75)μmol/L],而血清维生素D水平[(44.45±13.49)nmol/L]显著低于对照组[(71.57±15.71)nmol/L],差异有统计学意义(PoC曲线分析结果显示,单独应用指标进行诊断时,体重的灵敏度为44.4%,特异度为88.0%;BMI的灵敏度为59.3%,特异度为78.0%;维生素D的灵敏度为75.9%,特异度为94.0%;Hcy的灵敏度为68.9%,特异度为60.0%;4项指标进行联合诊断的灵敏度为88.9%,特异度为92.0%。此外,对照组、PCoS组及低维生素D组患者中,血清维生素D与Hcy呈负相关[相关系数(r)=-0.4291、-0.7516、-0.9182,PoS的灵敏度和特异度均优于单一指标。PCoS患者血清维生素D与Hcy呈负相关。因此,临床上可以考虑使用多项指标联合检测用于PCoS的诊断。

关键词：多囊卵巢综合征维生素D 同型半胱氨酸联合诊断

3D printing of functional nerve guide conduits

在线全文

学校读者我要写书评

暂无评论

Burns & Trauma 2021年第1期9卷 464-474页

作者： Yulan huang wenbi Wu Haofan Liu Yuwen Chen Bo Li Zhiyuan Gou Xun Li Maling Gou State Key Laboratory of Biotherapy and Cancer Center West China HospitalSichuan UniversityChengdu610041China Department of ophthalmology West China HospitalSichuan UniversityChengdu610041China

Nerve guide conduits(NGCs),as alternatives to nerve autografts and allografts,have been widely explored as an advanced tool for the treatment of peripheral nerve ***,the repairing efficiency of NGCs still needs significant *** NGCs that provide a more favorable microenvironment for promoting axonal elongation and myelination are of great *** recent years,3D printing technologies have been widely applied in the fabrication of customized and complex constructs,exhibiting great potential for tissue engineering applications,especially for the construction of functional *** this review,we introduce the 3D printing technologies for manufacturing functional NGCs,including inkjet printing,extrusion printing,stereolithographybased printing and indirect ***,we summarize the current methods and strategies for constructing functional NGCs,such as designing special conduit architectures,using appropriate materials and co-printing with different biological ***,the challenges and prospects for construction of functional NGCs are also presented.

关键词： Nerve guide conduits Functionalization Peripheral nerve repair 3D printing

血清同型半胱氨酸水平与HFpEF患者NT-proBNP水平及凝血功能的相关性

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中外医学研究 2024年第2期22卷 65-68页

作者：黄光秀廖思钦廖美艳池青罗冰邹文毕佛山市三水区人民医院广东佛山528100

目的:探讨血清同型半胱氨酸(Hcy)水平与射血分数保留的心力衰竭(HFpEF)患者N末端B型脑钠肽前体(NT-proBNP)水平及凝血功能的相关性。方法:选取2021年10月—2022年10月佛山市三水区人民医院收治的120例HFpEF患者为HFpEF组,选取同期正常... 详细信息

目的:探讨血清同型半胱氨酸(Hcy)水平与射血分数保留的心力衰竭(HFpEF)患者N末端B型脑钠肽前体(NT-proBNP)水平及凝血功能的相关性。方法:选取2021年10月—2022年10月佛山市三水区人民医院收治的120例HFpEF患者为HFpEF组,选取同期正常体检的120例患者为对照组,比较两组血清Hcy、凝血功能[凝血酶时间(TT)、活化部分凝血活酶时间(APTT)、凝血酶原时间(PT)、纤维蛋白原(FIB)]及NT-proBNP水平。采用Pearson相关性分析血清Hcy水平与HFpEF患者凝血功能、NT-proBNP水平的相关性。结果:HFpEF组NT-proBNP、Hcy、FIB、APTT、PT、TT均高于对照组,差异有统计学意义(Pon相关性分析显示,血清Hcy与HFpEF患者NT-proBNP、FIB、APTT、PT、PT呈正相关,差异有统计学意义(r=0.707、0.662、0.721、0.661、0.489,PoBNP、凝血功能水平呈正相关。

关键词：血清同型半胱氨酸 N末端B型脑钠肽前体射血分数保留的心力衰竭凝血功能相关性

维普期刊数据库博看期刊

Advances in high-performance MEMS pressure sensors:design,fabrication,and packaging

在线全文

学校读者我要写书评

暂无评论

Microsystems & Nanoengineering 2023年第6期9卷 43-76页

作者： Xiangguang Han Mimi huang Zutang Wu Yi Gao Yong Xia Ping Yang Shu Fan Xuhao Lu Xiaokai Yang Lin Liang wenbi Su Lu Wang Zeyu Cui Yihe Zhao Zhikang Li Libo Zhao Zhuangde Jiang State Key Laboratory for Manufacturing Systems Engineering Xi'an Jiaotong UniversityXi'an710049China International Joint Laboratory for Micro/Nano Manufacturing and Measurement Technologies Xi'an Jiaotong UniversityXi'an710049China School of Mechanical Engineering Xi'an Jiaotong UniversityXi'an710049China Northwest Institute of Nuclear Technology Xi'an710024China

Pressure sensors play a vital role in aerospace,automotive,medical,and consumer *** microelectromechanical system(MEMS)-based pressure sensors have been widely used for decades,new trends in pressure sensors,including higher sensitivity,higher accuracy,better multifunctionality,smaller chip size,and smaller package size,have recently *** demand for performance upgradation has led to breakthroughs in sensor materials,design,fabrication,and packaging methods,which have emerged frequently in recent *** paper reviews common new trends in MEMS pressure sensors,including minute differential pressure sensors(MDPSs),resonant pressure sensors(RPSs),integrated pressure sensors,miniaturized pressure chips,and leadless pressure *** realize an extremely sensitive MDPS with broad application potential,including in medical ventilators and fire residual pressure monitors,the“beam-membrane-island”sensor design exhibits the best performance of 66μV/V/kPa with a natural frequency of 11.3 *** high-accuracy applications,silicon and quartz RPS are analyzed,and both materials show±0.01%FS accuracy with respect to varying temperature coefficient of frequency(TCF)control *** improve MEMS sensor integration,different integrated“pressure+x”sensor designs and fabrication methods are *** this realm,the intercoupling effect still requires further *** fabrication methods for microsized pressure sensor chips are also *** date,the chip thickness size can be controlled to beous for implant ***,a leadless pressure sensor was analyzed,offering an extremely small package size and harsh environmental *** review is structured as *** background of pressure sensors is first ***,an in-depth introduction to MEMS pressure sensors based on different application scenarios is ***,their respective characteristics and significant advancem

关键词： performance breakthrough offering