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Autographa Californica Multiple Nucleopolyhedrovirus P48(Ac103) Is Required for the Efficient Formation of Virus-Induced Intranuclear Microvesicles

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Virologica Sinica 2019年第6期34卷 712-721页

作者： Yan Wang Qingyun Cai Jiannan Chen Zhihong Huang wenbi wu Meijin Yuan Kai Yang State Key Laboratory of Biocontrol Sun Yat-sen University

Our previous study has shown that the Autographa californica multiple nucleopolyhedrovirus(AcMNPV) p48(ac103)gene is essential for the nuclear egress of nucleocapsids and the formation of occlusion-derived virions(ODVs). However,the exact role of p48 in the morphogenesis of ODVs remains unknown. In this study, we demonstrated that p48 was required for the efficient formation of intranuclear microvesicles. To further understand its functional role in intranuclear microvesicle formation, we characterized the distribution of the P48 protein, which was found to be associated with the nucleocapsid and envelope fractions of both budded virions and ODVs. In Ac MNPV-infected cells, P48 was predominantly localized to nucleocapsids in the virogenic stroma and the nucleocapsids enveloped in ODVs, with a limited but discernible distribution in the plasma membrane, nuclear envelope, intranuclear microvesicles, and ODV envelope. Furthermore,coimmunoprecipitation assays showed that among the viral proteins required for intranuclear microvesicle formation, P48 associated with Ac93 in the absence of viral infection.

关键词： P48 Baculovirus Intranuclear microvesicle formation Protein association Ac93

3D printing of functional nerve guide conduits

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Burns & Trauma 2021年第1期9卷 464-474页

作者： Yulan Huang wenbi wu Haofan Liu Yuwen Chen Bo Li Zhiyuan Gou Xun Li Maling Gou State Key Laboratory of Biotherapy and Cancer Center West China HospitalSichuan UniversityChengdu610041China Department of Ophthalmology West China HospitalSichuan UniversityChengdu610041China

Nerve guide conduits(NGCs),as alternatives to nerve autografts and allografts,have been widely explored as an advanced tool for the treatment of peripheral nerve ***,the repairing efficiency of NGCs still needs significant *** NGCs that provide a more favorable microenvironment for promoting axonal elongation and myelination are of great *** recent years,3D printing technologies have been widely applied in the fabrication of customized and complex constructs,exhibiting great potential for tissue engineering applications,especially for the construction of functional *** this review,we introduce the 3D printing technologies for manufacturing functional NGCs,including inkjet printing,extrusion printing,stereolithographybased printing and indirect ***,we summarize the current methods and strategies for constructing functional NGCs,such as designing special conduit architectures,using appropriate materials and co-printing with different biological ***,the challenges and prospects for construction of functional NGCs are also presented.

关键词： Nerve guide conduits Functionalization Peripheral nerve repair 3D printing

Nerve transfer with 3D-printed branch nerve conduits

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Burns & Trauma 2022年第1期10卷 558-569页

作者： Jing Zhang Jie Tao Hao Cheng Haofan Liu wenbi wu Yinchu Dong Xuesong Liu Maling Gou Siming Yang Jianguo Xu Department of Neurosurgery National Clinical Research Center for GeriatricsWest China HospitalSichuan UniversityChengdu 610041P.R.China State Key Laboratory of Biotherapy and Cancer Center West China HospitalSichuan UniversityChengdu 610041P.R.China Department of Stomatology Daping HospitalThird Military Medical UniversityChongqing 400042P.R.China Key Laboratory of Wound Repair and Regeneration of PLA Chinese PLA General HospitalMedical College of PLABeijing 100853P.R.China

Background:Nerve transfer is an important clinical surgical procedure for nerve repair by the coaptation of a healthy donor nerve to an injured ***,nerve transfer is performed in an end-to-end manner,which will lead to functional loss of the donor *** this study,we aimed to evaluate the efficacy of 3D-printed branch nerve conduits in nerve ***:Customized branch conduits were constructed using gelatine-methacryloyl by 3D *** nerve conduits were characterized both in vitro and in *** efficacy of 3D-printed branch nerve conduits in nerve transfer was evaluated in rats through electrophysiology testing and histological ***:The results obtained showed that a single nerve stump could form a complex nerve network in the 3D-printed multibranch conduit.A two-branch conduit was 3D printed for transferring the tibial nerve to the peroneal nerve in *** this process,the two branches were connected to the distal tibial nerve and peroneal *** was found that the two nerves were successfully repaired with functional ***:It is implied that the two-branch conduit could not only repair the peroneal nerve but also preserve partial function of the donor tibial *** work demonstrated that 3D-printed branch nerve conduits provide a potential method for nerve transfer.

关键词： Branch nerve conduit Nerve transfer 3D printing Nerve regeneration

Introduction of temperature-sensitive helper and donor plasmids into Bac-to-Bac baculovirus expression systems

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Virologica Sinica 2015年第5期30卷 379-385页

作者： Zhihong Huang Ao Li Mengjia Pan wenbi wu Meijin Yuan Kai Yang State Key Laboratory of Biocontrol Sun Yat-sen University

In the baculovirus shuttle vector(bacmid) system, a helper plasmid and a donor plasmid are employed to insert heterologous genes into a cloned baculovirus genome via Tn7 transposition in Escherichia coli. The helper and donor plasmids are usually cotransfected with constructed bacmids into insect cells, which will lead to integration of these plasmids into the viral genome,and hence to the production of defective virions. In this study, to facilitate the preparation of plasmid-free recombinant bacmids, we modified a set of helper and donor plasmids by replacing their replication origins with that of a temperature-sensitive(ts) plasmid, p SIM6. Using the resulting ts helper plasmid p MON7124 ts and the ts donor plasmid p FB1ts-PH-GFP, a recombinant bacmid,b Ac WT-PG(-), was constructed, and the transposition efficiency was found to be 33.1%. The plasmids were then removed by culturing at 37 °C. For b Ac WT-PG(-), the infectious progeny virus titer and the protein expression level under the control of the polyhedrin promoter were similar to those of a bacmid constructed with unmodified helper and donor plasmids. These ts plasmids will be useful for obtaining plasmid-free bacmids for both heterologous protein production and fundamental studies of baculovirus biology.

关键词： baculovirus Bac-to-Bac baculovirus expression syst

ALGOR FEAS软件在铁道车辆结构分析中的应用

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铁道车辆 1996年第5期34卷 37-41页

作者：张文笔吴波长沙铁道学院长沙410075

介绍了 ALGOR FEAS 软件的特点及其在铁道车辆结构分析中的应用。

关键词：车辆结构分析有限元法软件铁路

结直肠癌生存者治疗结束3个月后重返正常生活现状调查

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护理研究 2024年第10期38卷 1833-1838页

作者：付练丁彩艳孙志琴吴文碧南京医科大学附属常州第二人民医院江苏213000 大连医科大学护理学院

目的:调查结直肠癌生存者治疗结束3个月后重返正常生活水平,并分析其影响因素。方法:采用便利抽样法,选取2022年11月—2023年6月在常州市2所三级甲等综合医院肿瘤科、胃肠外科住院及门诊复查的130例结直肠癌生存者为研究对象,采用一般... 详细信息

目的:调查结直肠癌生存者治疗结束3个月后重返正常生活水平,并分析其影响因素。方法:采用便利抽样法,选取2022年11月—2023年6月在常州市2所三级甲等综合医院肿瘤科、胃肠外科住院及门诊复查的130例结直肠癌生存者为研究对象,采用一般资料调查表、重返正常生活指数、社会支持评定量表、医院焦虑抑郁量表进行调查。结果:结直肠癌生存者治疗结束3个月后重返正常生活原始总分为(80.40±11.10)分,校正总分为(73.09±10.09)分。是否保肛、主观支持、焦虑、抑郁是结直肠癌生存者日常功能的影响因素,性别、客观支持、抑郁是结直肠癌生存者自我感知的影响因素。结论:结直肠癌生存者治疗结束3个月后重返正常生活受到中度限制,医护人员应重点关注未保肛、社会支持度较低、心理状况较差及女性结直肠癌生存者,实施个性化指导,促进结直肠癌生存者重返正常生活。

关键词：结肠癌直肠癌重返正常生活社会支持焦虑抑郁影响因素

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经皮肾镜联合输尿管软镜治疗复杂肾结石

中国微创外科杂志 2021年第10期21卷 875-878页

作者：张宝勋蒋胜利吴文弼赵永录费建功吴迪贾沛鹏吴旭甘肃省平凉市第二人民医院泌尿外科平凉744000 甘肃省武威市人民医院泌尿外科武威733000

目的探讨单通道经皮肾镜联合输尿管软镜治疗复杂肾结石的疗效。方法2019年10月~2020年12月,对37例复杂肾结石行单通道经皮肾镜EMS-Vario碎石清石联合输尿管软镜钬激光碎石,观察清石率、并发症发生率。结果37例手术均顺利完成,31例顺行碎... 详细信息

目的探讨单通道经皮肾镜联合输尿管软镜治疗复杂肾结石的疗效。方法2019年10月~2020年12月,对37例复杂肾结石行单通道经皮肾镜EMS-Vario碎石清石联合输尿管软镜钬激光碎石,观察清石率、并发症发生率。结果37例手术均顺利完成,31例顺行碎石,6例顺行+逆行碎石。手术时间50~140 min,平均100 min。出血量10~300 ml,平均70 ml。一期清石率91.9%(34/37),二期清石率97.3%(36/37),1例结石残留经体外冲击波碎石排出。术中并发症4例,包括集合系统黏膜损伤2例,大出血1例,冲洗液外渗1例。术后体温>38.5℃4例。随访3~6个月,无结石残留和并发症发生。结论经皮肾镜联合输尿管软镜治疗复杂性肾结石安全可行,可在有条件的医院推广使用。

关键词：经皮肾镜碎石输尿管软镜碎石复杂性肾结石清石率

网约护士执业过程风险感知现状及影响因素分析

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护理学杂志 2024年第3期39卷 95-99页

作者：吴文碧丁彩艳孙志琴朱青付练大连医科大学护理学院辽宁大连116000 南京医科大学附属常州第二人民医院护理教研室南京医科大学附属常州第二人民医院教育教学科南京医科大学附属常州第二人民医院护理部

目的了解江苏省网约护士风险感知现状及影响因素,为针对性干预提供参考。方法采用一般资料调查表、网约护士风险感知问卷、正负情绪量表、一般自我效能感量表、五大人格特质测量表对江苏省不同地区21所医院的219名网约护士进行调查。结... 详细信息

目的了解江苏省网约护士风险感知现状及影响因素,为针对性干预提供参考。方法采用一般资料调查表、网约护士风险感知问卷、正负情绪量表、一般自我效能感量表、五大人格特质测量表对江苏省不同地区21所医院的219名网约护士进行调查。结果网约护士风险感知总分为58.33±17.75分,得分率为41.66%;风险感知总分与负性情绪得分呈正相关,与一般自我效能得分、大五人格中的外向性、宜人性、严谨性、神经质得分均呈负相关(均P<0.05)。多元线性回归分析显示,外科、门诊岗位、近半年每单派出人数、近1年接单次数、风险熟悉程度、负性情绪、神经质为网约护士风险感知的影响因素(均P<0.05),可解释总变异的36.4%。结论江苏省网约护士风险感知处于偏低水平,受多种因素影响,管理者可针对影响因素实施干预,以提高网约护士风险感知水平,从而提高安全性。

关键词：网约护士风险感知正负情绪一般自我效能大五人格影响因素调查分析

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钢筋混凝土结构有限元网格剖分的一种方法

微机发展 2000年第3期10卷 64-66页

作者：张莉饶文碧吴锡琪武汉工业大学信息工程学院湖北省武汉市430070

本文将选用分离式模型对钢筋混凝土平面结构进行有限元网格剖分 ,其中对混凝土结构采用四边形网格的自动剖分方法 ,对钢筋则采用一维杆单元的生成方法。本文所述方法能保证所产生网格的质量 ,有利于钢筋混凝土有限元分析。

关键词：钢筋混凝土结构有限元网格剖分