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Rare Metals 2015年第9期34卷 632-637页

作者： Yu-Meng Luo Jin-Xu Liu Xing-Wang Cheng Shu-Kui Li Fu-Chi Wang wen-wen guo China National Key Laboratory of Science and Technology on Materials Under Shock and Impact School of Materials Science and Engineering Beijing Institute of Technology

Effect of stress state including dynamic shearing and uniaxial dynamic compression on adiabatic shear banding(ASBing) of hot-rolling Ti–6Al–4V(TC4) alloy was investigated. The absorbed energy of specimen before failure was calculated to evaluate the susceptibility to adiabatic shear band(ASB) of TC4 alloy *** show that the susceptibility to ASB of hot-rolling TC4 alloy exhibits obvious anisotropy under both dynamic shearing and uniaxial dynamic compression conditions, but the anisotropy of susceptibility to ASB under dynamic shearing condition exhibits an opposite tendency with that under uniaxial dynamic compression condition. Under the condition of uniaxial dynamic compression, material shows the highest susceptibility to ASB when loaded along transverse direction(TD) of the hot-rolling TC4, while the lowest susceptibility when loaded along rolling direction(RD). However, under the condition of dynamic shearing,the material behaves in the opposite way, demonstrating the lowest susceptibility when loaded along TD of the hotrolling TC4, while the highest susceptibility when loaded along RD.

关键词： Adiabatic shear banding Ti alloy Dynamic compressi

Germline promoter hypermethylation of tumor suppressor genes in gastric cancer

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World Journal of Gastroenterology 2012年第1期18卷 70-78页

作者： Pu-Yuan Wu Zheng Zhang Jing-Mei Wang wen-wen guo Nong Xiao Qiong He Ya-Ping Wang Yi-Mei Fan Department of Medical Genetics Medical SchoolNanjing University Nanjing 210093 Jiangsu Province China Jiangsu Key Laboratory of Molecular MedicineNanjing 210093 Jiangsu Province China Department of Pathology Drum Tower Hos-pital Affiliated to Medical School Nanjing University Nanjing210093 Jiangsu Province China Departmem of Pathology Lujiang Hos-pital Lujiang Chaohu 231500 Anhui Province China

AIM: To explore germline hypermethylation of the tumor suppressor genes MLH1, CDH1 and P16INK4a in suspected cases of hereditary gastric cancer (GC). METHODS: A group of 140 Chinese GC patients in whom the primary cancer had developed before the age of 60 or who had a familial history of cancer were screened for germline hypermethylation of the MLH1, CDH1 and P16INK4a tumor suppressor genes. GenomicDNA was extracted from peripheral blood leukocytes and modified by sodium bisulfite. The treated DNA was then subjected to bisulfi te DNA sequencing for a specif ic region of the MLH1 promoter. The methylation status of CDH1 or P16INK4a was assayed using methylation-specif ic PCR. Clonal bisulf ite allelic sequencing in positive samples was performed to obtain a comprehensive analysis of the CpG island methylation status of these promoter regions. RESULTS: Methylation of the MLH1 gene promoter was detected in the peripheral blood DNA of only 1/140 (0.7%) of the GC patient group. However, this methylation pattern was mosaic rather than the allelic pattern which has previously been reported for MLH1 in hereditary non-polyposis colorectal cancer (HNPCC) patients. We found that 10% of the MLH1 alleles in the peripheral blood DNA of this patient were methylated, consistent with 20% of cells having one methylated allele. No germline promoter methylation of the CDH1 or P16INK4a genes was detected. CONCLUSION: Mosaic germline epimutation of the MLH1 gene is present in suspected hereditary GC patients in China but at a very low level. Germline epimutation of the CDH1 or P16INK4a gene is not a frequent event.

关键词： Gastric cancer Germline promoter methylation MLH1 CDH1 P16INK4a

Cancer-testis Antigen OY-TES-1 Expression and Immunogenicity in Hepatocellular Carcinoma

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Current Medical Science 2020年第4期40卷 719-728页

作者： Bin LUO Xiang YUN Jing LI Rong FAN wen-wen guo Chang LIU Yong-da LIN Ying-ying GE Xia ZENG Shui-qing BI Wei-xia NONG Qing-mei ZHANG Xiao-xun XIE School of Basic Medical Sciences Guangxi Medical UniversityNanning 530021China Department of Surgery the First Affiliated HospitalGuangxi Medical UniversityNanning 530021China Clinical Laboratory Chinese Medicine HospitalQuanzhou 362000China School of Basic Medical Sciences Guangxi University of Chinese MedicineNanning 530021China Department of Pathology the People's Hospital of Guangxi Zhuang Autonomous RegionNanning 530021China Guangxi Key Laboratory of Early Prevention and Treatmentfor Regional High Frequency Tumor Guangxi Medical UniversityNanning 530021China Key Laboratory Research on Preclinical Medicine Guangxi Medical UniversityNanning 530021China

Summary:Cancer testis(CT)antigens have received particular attention in cancer ***-TES-1 is a member of CT *** study was to evaluate OY-TES-1 expression and immunogenicity in hepatocelluar carcinoma(HCC).OY-TES-1 mRNA expression was detected in 56 HCC tissues and 5 normal liver tissues by reverse transcriptase PCR(RT-PCR).Of the 56 cases of HCC tissues tested,37 cases had tumor and matched adjacent non-cancer tissues and were subjected to both RT-PCR and quantitative real-time ***-TES-1 protein was subsequently observed on a panel of tissue *** from patients were tested for OY-TES-1 antibody by *** identify OY-TES-1 capable of inducing cellular immune response,OY-TES-1 protein was used to sensitize dentritic cells and the cytotoxicity effect was measured in *** results showed that OY-TES-1 mRNA was highly expressed in 41 of the 56(73.21%)HCC tissues,whereas none in 5 normal liver ***-TES-1 mRNA was frequently expressed not only in HCC tissues(72.97%,27/37),but also in paired adjacent non-cancer tissues(64.86%,24/37).But the mean expression level of OY-TES-1 mRNA in HCC tissues was significantly higher than that in adjacent non-cancer tissues(0.76854 vs.0.09834,P=0.021).Immunohistochemistry showed that OY-TES-1 protein expression was detected in 6 of the 49 cases of HCC tissues,and absent in 9 cases of normal liver and 6 cases of cirrhosis *** was detected in 10 of the 45 HCC patients,but not detected in 17 cirrhosis patients and 76 healthy *** specific cytotoxic T cells elicited by OY-TES-1 could kill HLA-A2^+HCC cell line which expressed *** target lysis was mainly HLA class I-dependent and could be blocked by antibodies against monomorphic HLA class I but not HLA class II *** summary,OY-TES-1 expression is upregulated in HCC tissues and can be recognized by humoral and cellular responses,which suggests that OY-TES-1 is an attractive target for tumor immunotherapy in

关键词： cancer testis antigen hepatocelluar cancer tumor immunotherapy

Rhinocerebral mucormycosis caused by Rhizopus oryzae in a patient with acute myeloid leukemia: A case report

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World Journal of Dermatology 2020年第1期8卷 1-9页

作者： Ya-Hui Feng wen-wen guo Ya-Ru Wang wen-Xia Shi Chen Liu Dong-Mei Li Ying Qiu Dong-Mei Shi Department of Clinical Medicine Jining Medical UniversityJining 272067Shandong ProvinceChina Department of Hematology Jining No.1 People’s HospitalJining 272067Shandong ProvinceChina Department of Dermatology Jining No.1 People’s HospitalJining 272067Shandong ProvinceChina Laboratory of Clinical Mycology Jining No.1 People’s HospitalJining 272067Shandong ProvinceChina Medical Center Georgetown UniversityWashingtonDC 20057United States Laboratory of Medical Mycology Department of DermatologyJining No.1 People’s HospitalJining 272067Shandong ProvinceChina

BACKGROUND Rhinocerebral mucormycosis(RCM)is a rare fatal fungal infection which is on the increase among immunocompromised hosts such as patients who have had hematological cancers,or have received immunosuppressive drugs,corticosteroids,or other T cell suppressing *** SUMMARY We report a case of RCM caused by Rhizopus oryzae,one of the most common opportunistic pathogens,in a patient suffering from a fourth relapse of acute myeloid *** patient developed RCM after he had received long-term antibiotic agents and *** pathogen was isolated three times from nasal secretions collected from the deep parts of the nasal cavity and was identified by morphology and internal transcribed spacer *** infection was excluded by droplet digital polymerase chain reaction and blood *** patient was empirically treated with caspofungin and voriconazole for several days while the lesions continued to *** patient was given amphotericin B in combination with caspofungin after RCM was suspected,and the lesions improved over the course of treatment,which lasted several ***,the patient eventually died of the primary *** This case indicates that immunosuppressive drugs,including corticosteroids and antimetabolites in hematological tumor,do increase the risk of infections of this *** diagnosis,prompt and frequent surgical debridement,and treatment with amphotericin B without delay are all essential in combatting RCM.

关键词： Mucormycosis Rhinocerebral mucormycosis Rhizopus oryzae Acute myeloid leukemia Amphotericin B Droplet digital polymerase chain reaction Case report

The detection, extraction and parameter estimation of extreme-mass-ratio inspirals with deep learning

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Science China(Physics,Mechanics & Astronomy) 2025年第1期Mechanics & Astronomy) .卷 86-96页

作者： Qianyun Yun wen-Biao Han Yi-Yang guo He Wang Minghui Du Hangzhou Institute for Advanced Study University of Chinese Academy of Sciences Shanghai Astronomical Observatory Chinese Academy of Sciences School of Physics and Astronomy Shanghai Jiao Tong University School of Astronomy and Space Science University of Chinese Academy of Sciences Taiji Laboratory for Gravitational Wave Universe (Beijing/Hangzhou) University of Chinese Academy of Sciences Lanzhou Center for Theoretical Physics Key Laboratory of Theoretical Physics of Gansu Province Key Laboratory of Quantum Theory and Applications of MoE Lanzhou University Institute of Theoretical Physics & Research Center of Gravitation Lanzhou University International Centre for Theoretical Physics Asia-Pacific (ICTP-AP) University of Chinese Academy of Sciences (UCAS) Center for Gravitational Wave Experiment National Microgravity Laboratory Institute of Mechanics Chinese Academy of Sciences

One of the primary goals of space-borne gravitational wave detectors is to detect and analyze extreme-mass-ratio inspirals(EMRIs). This task is particularly challenging because EMRI signals are complex, lengthy, and faint. In this work, we introduce a2-layer convolutional neural network(CNN) approach to detect EMRI signals for space-borne detectors, achieving a true positive rate(TPR) of 96.9% at a 1% false positive rate(FPR) for signal-to-noise ratio(SNR) from 50 to 100. Especially, the key intrinsic parameters of EMRIs such as the mass, spin of the supermassive black hole(SMBH) and the initial eccentricity of the orbit can also be inferred directly by employing a neural network. The mass and spin of the SMBH can be determined at 99% and 92% respectively. This will greatly reduce the parameter spaces and computing cost for the following Bayesian parameter *** model also has a low dependency on the accuracy of the waveform model. This study underscores the potential of deep learning methods in EMRI data analysis, enabling the rapid detection of EMRI signals and efficient parameter estimation.

关键词： gravitational wave EMRIs deep-learing

Computed tomography-based nomogram of Siewert type Ⅱ/Ⅲ adenocarcinoma of esophagogastric junction to predict response to docetaxel, oxaliplatin and S-1

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World Journal of Radiology 2024年第1期16卷 9-19页

作者： Chuan-Qinyuan Zhou Dan Gao Yan Gui Ning-Pu Li wen-wen guo Hai-Ying Zhou Rui Li Jing Chen Xiao-Ming Zhang Tian-Wu Chen Department of Radiology Affiliated Hospital of North Sichuan Medical CollegeNanchong 637000Sichuan ProvinceChina Department of Oncology Affiliated Hospital of North Sichuan Medical CollegeNanchong 637000Sichuan ProvinceChina Department of Radiology Sichuan Provincial People's HospitalUniversity of Electronic Science and Technology of ChinaChengdu 610072Sichuan ProvinceChina Department of Radiology The Second Affiliated Hospital of Chongqing Medical UniversityChongqing 400010China

BACKGROUND Neoadjuvant chemotherapy(NAC)has become the standard care for advanced adenocarcinoma of esophagogastric junction(AEG),although a part of the patients cannot benefit from *** are no models based on baseline computed tomography(CT)to predict response of Siewert type II or III AEG to NAC with docetaxel,oxaliplatin and S-1(DOS).AIM To develop a CT-based nomogram to predict response of Siewert type II/III AEG to NAC with *** One hundred and twenty-eight consecutive patients with confirmed Siewert type II/III AEG underwent CT before and after three cycles of NAC with DOS,and were randomly and consecutively assigned to the training cohort(TC)(n=94)and the validation cohort(VC)(n=34).Therapeutic effect was assessed by disease-control rate and progressive disease according to the Response Evaluation Criteria in Solid Tumors(version 1.1)*** prognostic factors associated with responses after DOS treatment including Siewert classification,gross tumor volume(GTV),and cT and cN stages were evaluated using pretherapeutic CT data in addition to sex and *** and multivariate analyses of CT and clinical features in the TC were performed to determine independent factors associated with response to DOS.A nomogram was established based on independent factors to predict the *** predictive performance of the nomogram was evaluated by Concordance index(C-index),calibration and receiver operating characteristics curve in the TC and *** Univariate analysis showed that Siewert type(52/55 vs 29/39,P=0.005),pretherapeutic cT stage(57/62 vs 24/32,P=0.028),GTV(47.3±27.4 vs 73.2±54.3,P=0.040)were significantly associated with response to DOS in the *** analysis of the TC also showed that the pretherapeutic cT stage,GTV and Siewert type were independent predictive factors related to response to DOS(odds ratio=4.631,1.027 and 7.639,respectively;all P<0.05).The nomogram developed with these independent factors showed an exc

关键词： Esophagogastric junction Adenocarcinoma Neoadjuvant chemotherapy Response Tomography,X-ray computed Predictor

The potential mechanism and clinical application value of remote ischemic conditioning in stroke

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Neural Regeneration Research 2025年第6期20卷 1613-1627页

作者： Yajun Zhu Xiaoguo Li Xingwei Lei Liuyang Tang Daochen wen Bo Zeng Xiaofeng Zhang Zichao Huang Zongduo guo The First Affiliated Hospital of Chongqing Medical University ChongqingChina

Some studies have confirmed the neuroprotective effect of remote ischemic conditioning against stroke. Although numerous animal researches have shown that the neuroprotective effect of remote ischemic conditioning may be related to neuroinflammation, cellular immunity, apoptosis, and autophagy, the exact underlying molecular mechanisms are unclear. This review summarizes the current status of different types of remote ischemic conditioning methods in animal and clinical studies and analyzes their commonalities and differences in neuroprotective mechanisms and signaling pathways. Remote ischemic conditioning has emerged as a potential therapeutic approach for improving stroke-induced brain injury owing to its simplicity, non-invasiveness, safety, and patient tolerability. Different forms of remote ischemic conditioning exhibit distinct intervention patterns, timing, and application range. Mechanistically, remote ischemic conditioning can exert neuroprotective effects by activating the Notch1/phosphatidylinositol 3-kinase/Akt signaling pathway, improving cerebral perfusion, suppressing neuroinflammation, inhibiting cell apoptosis, activating autophagy, and promoting neural regeneration. While remote ischemic conditioning has shown potential in improving stroke outcomes, its full clinical translation has not yet been achieved.

关键词： Akt apoptosis autophagy cerebral perfusion cerebral vascular stenosis clinical transformation hemorrhagic stroke ischemic stroke neuroinflammation neuroprotection Notch1 PI3K remote ischemic conditioning stroke

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内质网应激对胃癌细胞迁移与侵袭的影响

世界华人消化杂志 2016年第10期24卷 1485-1491页

作者：董凯楠黄忻邢文英郭文文冯若郑州大学基础医学院组织学与胚胎学教研室河南省郑州市450001 郑州大学第三附属医院检验科河南省郑州市450052

目的:探讨内质网应激(endoplasmic reticulum stress,ERS)对胃癌细胞迁移及侵袭能力的影响及其机制.方法:5μg/mL衣霉素处理胃癌细胞系SGC7901或BGC823 0、12、24及36 h,Western blot检测各时间点葡萄糖调节蛋白78(glucoseregulated protein 78,GRP78)、金属基质蛋白酶9(matrix metalloproteinase 9,MMP9)及血管内皮细胞生长因子(vascular endothelial growth factor,VEGF)的表达;划痕实验检测衣霉素处理不同时间后对胃癌细胞迁移能力的影响,各时间点均设未加药对照组;Transwell侵袭实验检测衣霉素处理不同时间后对胃癌细胞侵袭能力的影响,各时间点均设未加药对照组.结果:与相应的0 h组相比,衣霉素处理胃癌细胞系SGC7901或BGC823 12、24及36 h后,GRP78、MMP9及VEGF的蛋白表达量均明显增加(P<0.05);衣霉素处理12、24及36 h后,胃癌细胞的划痕愈合度及胃癌细胞侵袭到Transwell下室的细胞数均明显高于0 h组(P<0.05).结论:ERS可上调MMP9及VEGF,促进胃癌细胞的迁移与侵袭.

关键词：内质网应激胃癌迁移侵袭

大鼠Shh-N融合蛋白的表达、纯化及功能研究

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生物化学与生物物理进展 2003年第6期30卷 969-973页

作者：孙朝晖赖燕来曾文文郭雅南左焕琮谢佐平清华大学生物科学与技术系生物膜与膜生物工程国家重点实验室北京100084

为了获得重组SonichedgehogN端蛋白 (Shh N)并研究其功能 ,应用PCR技术扩增Shh NcDNA ,然后克隆至原核表达质粒中 ,转化E .coli后获得表达菌株 ,经 1mmol/LIPTG诱导高效表达出带有His tag的融合蛋白 ,其中大部分为可溶性蛋白 ,少量为包... 详细信息

为了获得重组SonichedgehogN端蛋白 (Shh N)并研究其功能 ,应用PCR技术扩增Shh NcDNA ,然后克隆至原核表达质粒中 ,转化E .coli后获得表达菌株 ,经 1mmol/LIPTG诱导高效表达出带有His tag的融合蛋白 ,其中大部分为可溶性蛋白 ,少量为包涵体 .用His tag特异性结合树脂纯化可溶性融合蛋白 ,经SDS 聚丙烯酰胺凝胶电泳鉴定为单一区带 ,凝胶自动扫描分析表明 ,Shh N的纯度达 85%以上 .纯化后的Shh N在成纤维生长因子 8(FGF8)的协同作用下 ,能诱导神经前体细胞 (NPC)向酪氨酸羟化酶阳性神经元 (TH+ )发育 .在此基础上可以诱导不同类型的人类干细胞定向分化为多巴胺 (DA)神经元 ,从而为临床治疗帕金森病 (PD)

关键词：重组融合蛋白蛋白质纯化神经前体细胞 TH免疫阳性神经元

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有限长度编码的误比特率性能极限

通信学报 2001年第8期22卷 118-121页

作者：杨鸿文郭文彬庞沁华北京邮电大学北京100876

本文从码空间子集分解的角度出发，得到了加性高斯信道中二进制有限长度编码的误比特率下界。此极限表明码长大约在１０００ｂｉｔ以上时，好码的编码增益随码长增加的速度极为缓慢，并且在编码效率大约高于０．８时，编码增益也将急剧... 详细信息

本文从码空间子集分解的角度出发，得到了加性高斯信道中二进制有限长度编码的误比特率下界。此极限表明码长大约在１０００ｂｉｔ以上时，好码的编码增益随码长增加的速度极为缓慢，并且在编码效率大约高于０．８时，编码增益也将急剧下降。

关键词：有限长度编码信道编码误比特率性能极限