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Identification and immune analysis of antimicrobial peptides from the cigarette beetle(Lasioderma serricorne)

Insect Science 2024年第4期31卷 1121-1134页

作者： Yue Zhang Yi Yan Guy Smagghe Hong yang Ren-huai Dai wen-jia yang Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region Institute of EntomologyGuizhou UniversityGuiyangChina Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region College of Biology and Environmental EngineeringGuiyang UniversityGuiyangChina

Antimicrobial peptides(AMPs)in insects are endogenous peptides that are effector components of the innate defense system of the *** may serve as antimicrobial agents because of their small molecular weight and broad-spectrum antimicrobial *** this study,we performed transcriptome analysis of cigarette beetle(Lasioderma serricorne)larvae,parasitized by the ectoparasitic wasp,Anisopteromalus *** AMP genes were significantly upregulated following *** parasitism,postulating the hypothesis that the parasitization enhanced the host's resistance against pathogenic microorganisms through the regulation of host AMP ***,3 AMP genes(LsDef1,LsDef2,and LsCole)were significantly upregulated and we studied their immune function in *** challenge and functional analysis showed that LsCole was responsible for the immune response against Gram-negative and Gram-positive bacteria,while LsDef1 and LsDef2 were involved in insect defense against Gram-positive *** recombinant LsCole exhibited antimicrobial activities against the Gram-negative bacterium Escherichia coli and the Gram-positive bacterium Staphylococcus ***2 showed an antibacterial effect against *** and LsDef2 exhibited antibiofilm activity against *** 2 AMPs disrupted cell membranes and caused leakage of *** cell *** results indicated that the 3 AMPs in *** are involved in the innate immunity of this pest *** AMPs may have potential as antimicrobial agents for bacterial infection ***,data are discussed in relation to new control strategies with greater biosafety against pest insects with use of microbial biocontrol agents in combination with RNA interference against the insect's defensive AMP genes.

关键词： antimicrobial activity antimicrobial peptides innate immune response Lasioderma serricorne RNA interference

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MicroR-9c-5p and novel-mir50 co-target Akt to regulate Lasioderma serricorne reproduction

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Insect Science 2024年第1期31卷 106-118页

作者： Yi Yan Dong-Dong Qin Hong yang Kang-Kang Xu Can Li wen-jia yang Provincial Key Laboratory for Agricultural Pest Management of Mountainous Region Institute of EntomologyGuizhou UniversityGuiyangChina Guizhou Provincial Key Laboratory for Rare Animal and Economic Insect of the Mountainous Region College of Biology and Environmental EngineeringGuiyang UniversityGuiyangChina

High fecundity is a common characteristic of insect pests which increases the difficulty of population ***/threonine kinase Akt is an indispensable component of the insulin signaling *** of LsAkt severely hinders reproduction in Lasioderma serricorne,a stored product insect ***,the post-transcriptional pathway of LsAkt in *** remains *** study identified 2 binding sites of miR-9c-5p and novel-mir50 in the coding sequences of *** expression profiles of 2 microRNAs(miRNAs)and LsAkt displayed an opposite pattern during the adult *** reporter assay showed that novel-mir50 and miR-9c-5p could downregulate the expression of *** of miR-9c-5p and novel-mir50 by injection of mimics inhibited the expression of LsAkt and reduced oviposition,decreased egg hatchability,and blocked ovarian *** also decreased the expression of genes involved in ovarian development(LsVg and LsVgR)and the nutritional signaling pathway(LsTOR,LsS6K,and Ls4EBP),and reduced the phosphorylation of ***,injection of miR-9c-5p and novel-mir50 inhibitors induced the expressions of LsAkt,LsVg,LsVgR,LsTOR,LsS6K,and Ls4EBP,enhanced Akt phosphorylation level,and accelerated ovarian *** of bovine insulin downregulated the expression of miR-9c-5p and novel-mir50 and upregulated the LsAkt *** also rescued the reproductive development defects associated with miR-9c-5p/novel-mir50 overexpression,forming a positive regulatory loop of insulin *** results indicate that miR-9c-5p/novel-mir50 regulates the female reproduction of *** by targeting Akt in response to insulin *** data also demonstrate the effects of the insulin/miRNA/Akt regulatory axis in insect reproduction.

关键词： Akt insulin Lasioderma serricorne microRNA reproduction

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Pig H3K4me3,H3K27ac,and gene expression profiles reveal reproductive tissue-specific activity of transposable elements

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Zoological Research 2024年第1期45卷 138-151页

作者： Tao jiang Zhi-Min Zhou Zi-Qi Ling Qing Zhang Zhong-Zi Wu jia-wen yang Si-Yu yang Bin yang Lu-Sheng Huang National Key Laboratory of Pig Genetic Improvement and Germplasm Innovation Jiangxi Agricultural UniversityNanchangJiangxi 330045China

Regulatory sequences and transposable elements(TEs)account for a large proportion of the genomic sequences of species;however,their roles in gene transcription,especially tissue-specific expression,remain largely *** serve as an excellent animal model for studying genomic sequence biology due to the extensive diversity among their wild and domesticated ***,we conducted an integrated analysis using H3K27ac ChIP-seq,H3K4me3 ChIP-seq,and RNA-seq data from 10 different tissues of seven fetuses and eight closely related adult *** aimed to annotate the regulatory elements and TEs to elucidate their associations with histone modifications and mRNA expression across different tissues and developmental *** on correlation analysis between mRNA expression and H3K27ac and H3K4me3 peak activity,results indicated that H3K27ac exhibited stronger associations with gene expression than ***,1.45%of TEs overlapped with either the H3K27ac or H3K4me3 peaks,with the majority displaying tissue-specific ***,a TE subfamily(LTR4C_SS),containing binding motifs for SIX1 and SIX4,showed specific enrichment in the H3K27ac peaks of the adult and fetal ***-seq analysis also revealed widespread expression of TEs in the exons or promoters of genes,including 4688 TE-containing transcripts with distinct development stage-specific and tissue-specific *** note,1967 TE-containing transcripts were enriched in the *** identified a long terminal repeat(LTR),MLT1F1,acting as a testis-specific alternative promoter in SRPK2(a cell cycle-related protein kinase)in our pig *** element was also conserved in humans and mice,suggesting either an ancient integration of TEs in genes specifically expressed in the testes or parallel evolutionary ***,our findings demonstrate that TEs are deeply embedded in the genome and exhibit important tissue-specific biological functions,particularly in the reproductive

关键词： Transposable elements Porcine Histone modification Alternative promoter TE-containing transcript

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Residual tumor and central lymph node metastasis after thermal ablation of papillary thyroid carcinoma: A case report and review of literature

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World Journal of Clinical Cases 2021年第1期9卷 252-261页

作者： Yu Hua jia-wen yang Liu He Hua Xu Hai-Zhong Huo Chen-Fang Zhu Department of General Surgery Shanghai Ninth People's HospitalShanghai Jiao Tong University School of MedicineDiscipline Construction Research Center of China Hospital Development InstituteShanghai Jiao Tong UniversityShanghai 200011China

BACKGROUND Debate exists regarding the use of thermal ablation(TA)to treat papillary thyroid carcinoma(PTC).Some studies have recommended TA as a new,efcient and safe technology for *** this article,we report one case of a residual tumor and central lymph node metastasis(CLNM)after TA for *** SUMMARY A 63-year-old female underwent bilateral ultrasound(US)-guided radiofrequency ablation for *** months later,she was diagnosed as thyroid cancer with suspected CLNM by US and contrast-enhanced computed *** subsequent fine-needle aspiration(FNA)biopsies were *** to her strong personal preference,she underwent total thyroidectomy and central lymph node *** tissue adhesion and a difficult dissection were noted during the *** pathology of the frozen sections during the operation was still *** final pathology results of paraffin-embedded sections revealed residual tumor cells at the edge of the PTC and *** TA may lead to a residual tumor in patients with ***-up using US and FNA biopsy may not be adequate to evaluate the residual *** should be carefully considered in PTC treatment.

关键词： Papillary thyroid carcinoma Thermal ablation Residual tumor Central lymph node metastasis Follow-up Case report

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Treatment of leiomyomatosis peritonealis disseminata with goserelin acetate: A case report and review of the literature

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World Journal of Clinical Cases 2021年第19期9卷 5217-5225页

作者： jia-wen yang Yu Hua Hua Xu Liu He Hai-Zhong Huo Chen-Fang Zhu Department of General Surgery Shanghai Ninth People's HospitalShanghai Jiao Tong University School of MedicineDiscipline Construction Research Center of China Hospital Development InstituteShanghai Jiao Tong UniversityShanghai 200011China

BACKGROUND Leiomyomatosis peritonealis disseminata(LPD)is a rare condition characterized by multiple pelvic and abdominal nodules,which are composed of smoothmuscle *** date,no more than 200 cases have been *** diagnosis of LPD is difficult and there are no guidelines on the treatment of ***,surgical excision is the ***,hormone blockade therapy can be an alternative *** SUMMARY A 33-year-old female patient with abdominal discomfort and palpable abdominal masses was admitted to our *** had undergone four surgeries related to uterine leiomyoma in the past 8 *** tomography revealed multiple nodules scattered within the abdominal wall and peritoneal *** symptoms and the result of the core-needle biopsy were consistent with *** patient refused surgery and was then treated with tamoxifen,ulipristal acetate(a selective progesterone receptor modulator),and goserelin acetate(a gonadotropin-releasing hormone agonist).Both tamoxifen and ulipristal acetate were not effective in controlling the disease ***,the patient achieved an excellent response when goserelin acetate was attempted with relieved syndromes and obvious shrinkage of *** largest nodule showed a 25%decrease in the sum of the longest diameters from pretreatment to *** to now,2 years have elapsed and the patient remains asymptomatic and there is no development of further *** Goserelin acetate is effective for the management of *** long-term use of goserelin acetate is thought to be safe and *** blockade therapy can replace repeated surgical excision in recurrent patients.

关键词： Goserelin acetate Leiomyomatosis peritonealis disseminata Hormone blockade therapy Conservative treatment Disseminated peritoneal leiomyomatosis Case report

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Single-cell profiling of the pig cecum at various developmental stages

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Zoological Research 2024年第1期45卷 55-68页

作者： Yan-Yuan Xiao Qing Zhang Fei Huang Lin Rao Tian-Xiong Yao Si-Yu yang Lei Xie Xiao-Xiao Zou Li-Ping Cai jia-wen yang Bin yang Lu-Sheng Huang National Key Laboratory for Swine Genetic Improvement and Germplasm Innovation Ministry of Science and Technology of ChinaJiangxi Agricultural UniversityNanchangJiangxi 330045China

The gastrointestinal tract is essential for food digestion,nutrient absorption,waste elimination,and microbial ***-cell transcriptome profiling of the intestinal tract has greatly enriched our understanding of cellular diversity,functional heterogeneity,and their importance in intestinal tract development and *** such profiling has been extensively conducted in humans and mice,the single-cell gene expression landscape of the pig cecum remains ***,single-cell RNA sequencing was performed on 45572 cells obtained from seven cecal samples in pigs at four different developmental stages(days(D)30,42,150,and 730).Analysis revealed 12 major cell types and 38 subtypes,as well as their distinctive genes,transcription factors,and regulons,many of which were conserved in *** increase in the relative proportions of CD8^(+)T and Granzyme A(low expression)natural killer T cells(GZMA^(low)NKT)cells and a decrease in the relative proportions of epithelial stem cells,Tregs,RHEX^(+)T cells,and plasmacytoid dendritic cells(pDCs)were noted across the developmental ***,the post-weaning period exhibited an up-regulation in mitochondrial genes,COX2 and ND2,as well as genes involved in immune activation in multiple cell ***-cell crosstalk analysis indicated that IBP6^(+)fibroblasts were the main signal senders at D30,whereas IBP6^(−)fibroblasts assumed this role at the other *** cells established interactions with epithelial cells and IBP6^(+)fibroblasts in the D730 cecum through mediation of GZMA-F2RL1/F2RL2 *** study provides valuable insights into cellular heterogeneity and function in the pig cecum at different development stages.

关键词： Single-cell RNA-seq Cecum Bama Xiang pigs Various developmental stages Cellular heterogeneity

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Influence of various stressors on the expression of core genes of the small interfering RNA pathway in the oriental fruit fly, Bactrocera dorsalis

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Insect Science 2017年第3期24卷 418-430页

作者： Yi-Fei Xie Jin-Zhi Niu Xuan-Zhao jiang wen-jia yang Guang-Mao Shen Dong Wei Guy Smagghe Jin-Jun Wang Key Laboratory of Entomology and Pest Control Engineering College of Plant Protection Southwest University Chongqing China Department of Crop Protection Faculty of Bioscience Engineering Ghent University Ghent Belgium Hunan Academy of Forestry Changsha China

RNA interference （RNAi）-based technology has emerged as a potential tool for controlling insect pests, however, previous studies found that the efficiency of RNAi in Bactrocera dorsalis was variable. In nature, insects often meet various challenges, such as pathogen infections, extreme temperatures, lack of nutrition and heavy metals. To better understand the association of the stressors with efficiency of RNAi, in the current study we tested the expression of three core genes, dicer2 （Bddcr2）, r2d2 （Bdr2d2） and argonaute2 （Bdago2）, of the small interfering RNA （siRNA） pathway of B. dorsalis upon various stressors. Our results showed that all three genes were upregulated by the infection of invertebrate iridescent virus 6, which suggested a function of the siRNA pathway against viral infection. The loading of FeC13 could also increase the expression of Bddcr2. The treatments ofEscherichia coli, extremely high （40~C） and low （0~C） temperatures, as well as starvation, could negatively influence the expression of Bddcr2 and/or Bdago2. In total, our results showed that various stressors could influence the expression of core components ofB. dorsalis siRNA pathway. This highlights further speculation on the RNAi efficiency upon these stressors. Considering the complexity and variation of RNAi efficiency in different conditions, these results provide initial aspects in possible environmental stressors to influence the activity of the siRNA pathway, but the real impact of RNAi efficiency posed by these stressors requires further studies.

关键词： argonaute Bactrocera dorsalis dicer RNA interference R2D2 stressor

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Novel glucose-lowering drugs for non-alcoholic fatty liver disease

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World Journal of Diabetes 2021年第1期12卷 84-97页

作者： Zuo-Di Fu Xiao-Ling Cai wen-jia yang Ming-Ming Zhao Ran Li Yu-Feng Li Department of Endocrinology Beijing Friendship Hospital Pinggu CampusBeijing 101200China Department of Endocrinology and Metabolism Peking University People’s HospitalBeijing 100044China The Institute of Cardiovascular Sciences School of Basic Medical SciencesHealth Science CenterPeking UniversityBeijing 100079China Sport Science School Beijing Sport UniversityBeijing 100078China Department of Endocrinology Beijing Friendship Hospital Pinggu CampusCapital Medical UniversityBeijing 101200China

BACKGROUND The efficacy of novel glucose-lowering drugs in treating non-alcoholic fatty liver disease(NAFLD)is *** To evaluate the efficacy of glucose-lowering drugs dipeptidyl peptidase-4(DPP-4)inhibitors,glucagon-like peptide-1 receptor agonists(GLP-1 RAs),and sodiumglucose cotransporter 2(SGLT2)inhibitors in treating NAFLD and to perform a comparison between these *** Electronic databases were systematically *** inclusion criteria were:Randomized controlled trials comparing DPP-4 inhibitors,GLP-1 RAs,or SGLT2 inhibitors against placebo or other active glucose-lowering drugs in NAFLD patients,with outcomes of changes in liver enzyme[alanine aminotransferase(ALT)and/or aspartate aminotransferase(AST)]from *** Nineteen studies were finally included in this *** with placebo or other active glucose-lowering drug treatment,treatment with DPP-4 inhibitors,GLP-1 RAs,and SGLT2 inhibitors all led to a significant decrease in ALT change and AST change from *** difference between the DPP-4 inhibitor and SGLT2 inhibitor groups in ALT change was significant in favor of DPP-4 inhibitor treatment(P<0.05).The trends of reduction in magnetic resonance imaging proton density fat fraction and visceral fat area changes were also observed in all the novel glucose-lowering agent treatment *** Treatment with DPP-4 inhibitors,GLP-1 RAs,and SGLT2 inhibitors resulted in improvements in serum ALT and AST levels and body fat composition,indicating a beneficial effect in improving liver injury and reducing liver fat in NAFLD patients.

关键词： Non-alcoholic fatty liver disease Glucose-lowering drug Meta-analysis Dipeptidyl peptidase-4 inhibitor Sodium-glucose cotransporter 2 inhibitor Glucagon-like peptide-1 receptor agonist

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Indirect comparison of efficacy and safety of chiglitazar and thiazolidinedione in patients with type 2 diabetes:A meta-analysis

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World Journal of Diabetes 2023年第10期14卷 1573-1584页

作者： Chu Lin Zong-Lin Li Xiao-Ling Cai Sui-Yuan Hu Fang Lv wen-jia yang Li-Nong Ji Department of Endocrinology and Metabolism Peking University People's HospitalBeijing 100044China

BACKGROUND Chiglitazar is an emerging pan-agonist of all peroxisome proliferator activated receptors(PPAR)-α,δandγ,and has therapeutic potential for type 2 diabetes(T2D).However,to date,no clinical studies or meta-analyses have compared the efficacy and safety of chiglitazar and traditional PPAR-γagonist thiazolidinediones(TZDs).A meta-analysis concerning this topic is therefore *** To compare the efficacy and safety of chiglitazar and TZD in patients with *** PubMed,Medline,Embase,the Cochrane Central Register of Controlled Trials,Reference Citation Analysis and *** websites were searched from August 1994 to March *** controlled trials(RCTs)of chiglitazar or TZD vs placebo in patients with T2D were *** comparisons and sensitivity analyses were implemented to evaluate multiple efficacy and safety endpoints of *** We included 93 RCTs that compared TZD with placebo and one that compared chiglitazar with *** efficacy endpoints,the augmented dose of chiglitazar resulted in greater reductions in hemoglobin(Hb)A1c[weighted mean difference(WMD)=-0.15%,95%confidence interval(CI):-0.27 to-0.04%],triglycerides(WMD=-0.17 mmol/L,95%CI:-0.24 to-0.11 mmol/L)and alanine aminotransferase(WMD=-5.25 U/L,95%CI:-8.50 to-1.99 U/L),and a greater increase in homeostasis model assessment-β(HOMA-β)(WMD=17.75,95%CI:10.73-24.77)when compared with TZD *** safety endpoints,the risks of hypoglycemia,edema,bone fractures,upper respiratory tract infection,urinary tract infection,and weight gain were all comparable between the augmented dose of chiglitazar and *** patients with baseline HbA1c≥8.5%,body mass index≥30 kg/m^(2)or diabetes duration<10 years,the HbA1c reduction and HOMA-βincrease were more conspicuous for the augmented dose of chiglitazar compared with *** Augmented dose of chiglitazar,a pan-activator of PPARs,may serve as an antidiabetic agent with preferable glycemic and lipid control

关键词： Chiglitazar Thiazolidinedione Glycemic control β-cell function Drug safety

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Distribution of Major Pathogens from Sputum and Bronchoalveolar Lavage Fluid in Patients with Noncystic Fibrosis Bronchiectasis： A Systematic Review

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Chinese Medical Journal 2015年第20期128卷 2792-2797页

作者： Xia-Yi Miao Xiao-Bin Ji Hai-wen Lu jia-Wei yang Jin-Fu Xu Department of Respiratory Medicine Shanghai Pulmonary Hospital Tongji University School of Medicine Shanghai 200433 China

Objective： Noncystic fibrosis （non-CF） bronchiectasis remains as a common health problem in Asia. Pathogens＇ distribution in airways of patients with non-CF bronchiectasis is important for doctors to make right decision. Data Sources： We performed this systematic review on the English language literatures from 1966 to July 2014, using various search terms included ＂pathogens＂ or ＂bacteria＂ or ＂microbiology＂ and ＂bronchiectasis＂ or ＂non-cystic fibrosis bronchiectasis＂ or ＂non-CF bronchiectasis＂ or ＂NCFB.＂ Study Selection： We included studies of patients with the confirmed non-CF bronchiectasis for which culture methods were required to sputum or bronchoalveolar lavage fluid （BALF）. Weighted mean isolation rates for Haemophilus influenzae, Pseudomonas aeruginosa, Streptococcus pneumoniae, Stapylococcus aureus, Moxarella catarrhails were compared according to different methodology. Results： The total mean bacterial culture positive rates were 63%. For studies using sputum samples, the mean positive culture rates were 74%. For studies using BALF alone or BALF and sputum, it was 48%. The distributions of main bacterial strains were 29% for 1-1. influenzae, 28% for P. aeruginosa, 11% for S. pneumoniae, 12% for S. aureus, and 8% for M. catarrhails with methodology of sputum. Meanwhile, the bacterial distributions were 37% for H. influenzae, 8% for P aeruginosa, 14% for S. pneumoniae, 5% for S. attretts, and 10% for M. catarrhails with methodology of BALF alone or BALF and sputum. Analysis of the effect of different methodology on the isolation rates revealed some statistically significant differences. Conclusions： H. influenzae accounted for the highest percentage in different methodology. Our results suggested that the total positive culture rates and the proportion of P. aeruginosa from sputum and BALF specimens had significant differences, which can be used in further appropriate recommendations for the treatment of non-CF bronchiectasis.

关键词： Bronchiectasis Bronchoalveolar Lavage Fluid Pathogens Sputum

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