Single-cell profiling of the pig cecum at various developmental stages

作     者：Yan-Yuan Xiao Qing Zhang Fei Huang Lin Rao Tian-Xiong Yao Si-Yu Yang Lei Xie Xiao-Xiao Zou Li-Ping Cai Jia-Wen Yang Bin Yang Lu-Sheng Huang Yan-Yuan Xiao;Qing Zhang;Fei Huang;Lin Rao;Tian-Xiong Yao;Si-Yu Yang;Lei Xie;Xiao-Xiao Zou;Li-Ping Cai;Jia-Wen Yang;Bin Yang;Lu-Sheng Huang

作者机构：National Key Laboratory for Swine Genetic Improvement and Germplasm InnovationMinistry of Science and Technology of ChinaJiangxi Agricultural UniversityNanchangJiangxi 330045China 

出 版 物：《Zoological Research》 (动物学研究（英文）)

年 卷 期：2024年第45卷第1期

页      面：55-68页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：supported by the National Natural Science Foundation of China(31790410 32160781) 

主　　题：Single-cell RNA-seq Cecum Bama Xiang pigs Various developmental stages Cellular heterogeneity 

摘      要：The gastrointestinal tract is essential for food digestion,nutrient absorption,waste elimination,and microbial ***-cell transcriptome profiling of the intestinal tract has greatly enriched our understanding of cellular diversity,functional heterogeneity,and their importance in intestinal tract development and *** such profiling has been extensively conducted in humans and mice,the single-cell gene expression landscape of the pig cecum remains ***,single-cell RNA sequencing was performed on 45572 cells obtained from seven cecal samples in pigs at four different developmental stages(days(D)30,42,150,and 730).Analysis revealed 12 major cell types and 38 subtypes,as well as their distinctive genes,transcription factors,and regulons,many of which were conserved in *** increase in the relative proportions of CD8^(+)T and Granzyme A(low expression)natural killer T cells(GZMA^(low)NKT)cells and a decrease in the relative proportions of epithelial stem cells,Tregs,RHEX^(+)T cells,and plasmacytoid dendritic cells(pDCs)were noted across the developmental ***,the post-weaning period exhibited an up-regulation in mitochondrial genes,COX2 and ND2,as well as genes involved in immune activation in multiple cell ***-cell crosstalk analysis indicated that IBP6^(+)fibroblasts were the main signal senders at D30,whereas IBP6^(−)fibroblasts assumed this role at the other *** cells established interactions with epithelial cells and IBP6^(+)fibroblasts in the D730 cecum through mediation of GZMA-F2RL1/F2RL2 *** study provides valuable insights into cellular heterogeneity and function in the pig cecum at different development stages.