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3,4-Dinitrobenzamide Functionalized CdTe/ZnTe Quantum Dots as a Nanoprobe for Imaging Glutathione S-Transferase in living Cells

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Chinese Journal of Chemistry 2013年第4期31卷 472-478页

作者： Jinxin Lu Yanchao Song wen shi Xiaohua li Huimin Ma Beijing National Laboratory for Molecular Sciences Key Laboratory of Analytical Chemistry for Living Biosystems Institute of Chemistry Chinese Academy of Sciences Beijing 100190 China

A novel fluorescent nanoprobe for glutathione S-transferase （GST） has been developed by incorporating 3,4-dinitrobenzamide （a specific substrate of GST） onto CdTe/ZnTe quantum dots. The probe itself displays a low background signal due to the strong quenching effect of the electron-withdrawing unit of 3,4-dinitrobenzamide on the quantum dots. However, GST can efficiently catalyze the nucleophilic substitution of reduced glutathione on the p-nitro group of the nanoprobe, leading to a large fluorescence enhancement. Most notably, this enhancement shows high selectivity and sensitivity towards GST instead of the other biological substances. With this nanoprobe, a simple fluorescence imaging method for intracellular GST has been established, and its applicability has been successfully demonstrated for imaging GST in different living cells, which reveals that A549 cells express GST about 3 times higher than NIH-3T3 and Hela cells.

关键词： glutathione S-transferase fluorescence nanoprobe quantum dots bioimaging

Note on 2-edge-colorings of complete graphs with small monochromatic k-connected subgraphs

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Applied Mathematics(A Journal of Chinese Universities) 2014年第2期29卷 249-252页

作者： JIN Ze-min WANG Yu-ling wen shi-li Department of Mathematics Zhejiang Normal University

Bollobas and Gyarfas conjectured that for n 〉 4（k - 1） every 2-edge-coloring of Kn contains a monochromatic k-connected subgraph with at least n - 2k ＋ 2 vertices. liu, et al. proved that the conjecture holds when n 〉 13k - 15. In this note, we characterize all the 2-edge-colorings of Kn where each monochromatic k-connected subgraph has at most n - 2k ＋ 2 vertices for n ≥ 13k - 15.

关键词： monochromatic subgraph k-connected subgraph 2-edge-coloring.

Cold-state spout-fluidizing characteristics of high-carbon ferromanganese powders

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International Journal of Minerals,Metallurgy and Materials 2011年第6期18卷 741-747页

作者： Jin Chen wen-li shi Jing Zhao Wan-ming lin Ke liu College of Materials Science and Engineering Taiyuan University of Technology Taiyuan 030024 China Xiangtan Science and Technology Co. Ltd. Xiangtan 411100 China

The spout-fluidizing characteristics of high-carbon ferromanganese powders with different sizes and masses were studied via a plexiglass spout-fluidized bed with an inner diameter of 30 mm and a height of 1000 *** relationships between bed voidage and such parameters as bed height,particle size,fluidizing air velocity,and air flow were *** results show that the powder material with high density can be fluidized in the spout-fluidized bed where the particle size is a key factor influencing the quality of fluidization.

关键词： ferromanganese powders fluidity density fluidized beds

Theoretical study on the reaction of VS^+(~3∑^-,~1Γ)with CO

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Chinese Chemical Letters 2008年第8期19卷 1008-1011页

作者： shi wen Yu li Qing Yin li Feng Yao Ming li Xiao Guang Xie Department of Chemistry Yunnan University Kunming 650091 China

Two possible reaction mechanisms of VS^＋（^3∑^-, 1^Г） with CO in the gas phase have been studied by using B3LYP/TZVP and CCSD（T）/6-311＋G （3df, 3pd） methods： the O/S exchange reaction （VS^＋＋CO→VO^＋＋CS） and the S-transfer reaction （VS^＋＋ CO → V^＋＋ COS）. The two reactions proceed via two-step and one-step mechanism, respectively. The barriers of the triplet and singlet PESs are 30.6 and 50.9 kcal/mol, respectively, for O/S exchange reaction and 7.3 and 50.2 kcal/mol, respectively, for the S-transfer reaction. The results indicate that the triplet ground state reaction is more favorable, and the S-transfer reaction is more favorable than the O/S exchange reaction, which is in good agreement with the experimental observation.

关键词： Vanadium sulfide ion CO Reaction CCSD(T)

Solid-Phase Decarburization of High-Carbon Ferromanganese Powders by Microwave Heating

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Journal of Iron and Steel Research International 2013年第8期20卷 34-40页

作者： GUO li-na CHEN Jin shi wen-li ZHAO Jing liU Ke liU Jin-ying College of Materials Science and Engineering Taiyuan University of Technology lian-zhi Technology Corporation of Xiangtan City No.12 Research Institute China Electronics Technology Group Corporation

The solid-phase decarburization of high-carbon ferromanganese powders （HCFPs） was investigated using calcium carbonate as the decarburizer by microwave heating and conventional heating methods to explore the differ-ences of microwave heating and conventional heating. Experimental results show that HCFPs containing ***-bonate were heated up to 900, 1000, 1 100, and 1200 ℃ and held for 60 rain for decarburization by microwave heat-ing at decarburization ratios of 76.69%, 82.90%, 84.11%, and 85. 75%, respectively. These ratios are higher than the decarburization ratios used for conventional heating under the same experimental conditipns. The microwave heat- ing can significantly improve decarburization ratio. This indicates the microwave heating field features a non-thermal effect, which in turn, visibly enhances the carbon diffusion ability of HCFPs. It also improves the kinetic conditions of solid-phase decarburization.

关键词： high-carbon ferromanganese powder solid-phase decarburization microwave heating conventional heating

Solid-phase Decarburization Kinetics of High-carbon Ferromanganese Powders by Microwave Heating

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Journal of Iron and Steel Research International 2015年第4期22卷 311-316页

作者： li-na GUO Jin CHEN Jing ZHAO Wan-ming liN wen-li shi Min liANG College of Materials Science and Engineering Taiyuan University of Technology

Solid-phase decarburization of high-carbon ferromanganese powders （HCFPs） was conducted using calcium carbonate powders （CCPs） as a decarburizer by microwave heating. Solid-phase decarburization kinelSics was investi- gated by isothermal method. The results show that the HCFPs show excellent microwave absorption at a higher av- erage heating rate of 80 ℃/min, while CCPs exhibit poor microwave absorption at a lower heating rate of 5--20 ℃/min; the heating characteristics are in-between when HCFPs and CCPs are mixed. The average heating rates of the mix- ture are 32.14, 31.25, 31.43, and 30.77 ℃/rain when the mixture is heated up to 900, 1000, 1100, and 1200 ℃, respectively. The good microwave absorption property of the mixed material lays the foundation for the solid-phase decarburization of HCFPs containing CCPs. Solid-phase decarburization of HCFPs containing CCPs is a first-order reaction by microwave heating. Apparent activation energy of solid-phase decarburization is 55.07 kJ/mol, which is far less than that of ordinary carbon gasification reaction and that of solid-phase decarburization under the same de- carburization condition by conventional heating. It indicates that microwave heating not only produces thermal effect, but also has non-thermal effect.

关键词： high-carbon ferromanganese powder solid-phase decarburization kinetics microwave heating calcium carbonate powder

Cavernous nerve reconstruction with autologous vein graft and platelet-derived growth factors

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Asian Journal of Andrology 2017年第3期19卷 298-302页

作者： Xie-Gang Ding shi-wen li Xin-Min Zheng Xing-Huan Wang Yi Luo Department of Urology Zhongnan Hospital Wuhan University Wuhan 430071 China.

In this study, we investigated the feasibility of using autologous vein graft and platelet-derived growth factors to bridge transected cavernous nerve in a rat model. A short defect in the bilateral cavernous nerve was created and repaired with vein graft from the right jugular vein or vein graft plus platelet-derived growth factors. The 32 rats were divided into four groups, namely Group 1 - no repair as a negative control, Group 2 - vein graft alone, Group 3 - vein graft plus platelet-derived growth factors, and Group 4 - sham operation as a positive control. We evaluated nerve regeneration and functional recovery using retrograde tracing study with FluoroGold, Toluidine blue staining of cavernous nerve, and the intracavernous pressure at 3 months. Three months after surgery, rich FluoroGold-positive cells were observed in the sham and vein graft plus platelet-derived growth factors group, but very few were found in the no repair group. The number of myelinated axons of regenerated cavernous nerve and intracavernous pressure were increased obviously in the two vein graft groups, especially in the vein graft plus platelet-derived growth factors group. These findings confirm the feasibility of using autologous vein as guides for cavernous nerve regeneration, and the regeneration can be further enhanced when the vein is filled with Dlatelet-derived growth factors.

关键词： cavernous nerve nerve regeneration platetet-rich plasma vein graft

CPEB1,a novel risk gene in recent-onset schizophrenia,contributes to mitochondrial complex I defect caused by a defective provirus ERVWE1

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World Journal of Psychiatry 2021年第11期11卷 1075-1094页

作者： Ya-Ru Xia Xiao-Cui Wei wen-shi li Qiu-Jin Yan Xiu-lin Wu Wei Yao Xu-Hang li Fan Zhu State Key Laboratory of Virology and Hubei Province Key Laboratory of Allergy&Immunology Department of Medical MicrobiologySchool of MedicineWuhan UniversityWuhan 430071Hubei ProvinceChina

BACKGROUND Schizophrenia afflicts 1%of the world *** studies suggest that schizophrenia patients may have an imbalance of mitochondrial energy metabolism via inhibition of mitochondrial complex I ***,recent studies have shown that ERVWE1 is also a risk factor for ***,there is no available literature concerning the relationship between complex I deficits and ERVWE1 in *** risk factors and blood-based biomarkers for schizophrenia may provide new guidelines for early interventions and prevention *** To address novel potential risk factors and the underlying mechanisms of mitochondrial complex I deficiency caused by ERVWE1 in *** Quantitative polymerase chain reaction(qPCR)and enzyme-linked immunosorbent assay were used to detect differentially expressed risk factors in blood *** statistical analyses were performed by median analyses and Mann-Whitney U ***’s rank correlation was applied to examine the correlation between different risk factors in blood ***,western blot analysis,and luciferase assay were performed to confirm the relationship among ERVWE1,cytoplasmic polyadenylation element-binding protein 1(CPEB1),NADH dehydrogenase ubiquinone flavoprotein 2(NDUFV2),and NDUFV2 pseudogene(NDUFV2P1).The complex I enzyme activity microplate assay was carried out to evaluate the complex I activity induced by *** Herein,we reported decreasing levels of CPEB1 and NDUFV2 in schizophrenia *** studies showed that ERVWE1 was negatively correlated with CPEB1 and NDUFV2 in ***,NDUFV2P1 was increased and demonstrated a significant positive correlation with ERVWE1 and a negative correlation with NDUFV2 in *** vitro experiments disclosed that ERVWE1 suppressed NDUFV2 expression and promoter activity by increasing NDUFV2P1 *** luciferase assay revealed that ERVWE1 could enhance the promoter

关键词： ERVWE1 CPEB1 NADH dehydrogenase ubiquinone flavoprotein 2 complex I Pseudogene

Research on Qualitative Model Decomposition

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International Journal of Plant Engineering and Management 2010年第1期15卷 1-12页

作者： XIA wen-jun li shi-qi liU shi-ping Department of Industrial and Manufacturing System Engineering School of Mechanical Science and Engineering Huazhong University of Science and Technology Wuhan 430074 China

Qualitative reasoning uses incomplete knowledge to compute a description of the possible behaviors for dynamic systems. A standard qualitative simulation（QSIM） algorithm frequently results in a large number of incomprehensible behavioral descriptions and the simulation for complex systems frequently is intractable. Two model de- composition methods are proposed in this paper to eliminate or decrease the insujficiency of this algorithm. Using a directed graph to represent the qualitative model, the strongly connected graph based theory and genetic algorithm based model decomposition are proposed to decompose the model. A new simple system model is reconstructed by subgraphs and causal relations when the system directed graph is decomposed completely. Each sub-graph is viewed as a separate system and will be simulated separately, and the simulation result of causally upstream subsystem is used to constrain the behavior of downstream subsystems. The model decomposition algorithm provides a promising paradigm for qualitative simulation whose complexity is driven by the complexity of the problem specification rather than the inference mechanism used.

关键词： qualitative simulation model decomposition directed graph causal relation