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玉米碳水化合物分配缺陷突变体ygl3的光合特性及遗传分析

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玉米科学 2024年第3期32卷 32-41页

作者：刘忠祥李永生王晓娟连晓荣梁根生寇向龙何海军杨彦忠周文期周玉乾甘肃省农业科学院作物研究所兰州730070 甘肃省农业科学院农业质量标准与检测技术研究所兰州730070

以快中子辐射诱变玉米自交系PH6WC筛选得到的碳水化合物分配缺陷突变体ygl3为材料,通过叶片内叶绿素含量和碳水化合物含量的测定、叶绿体结构显微观察、光合特性和遗传特性等进行分析。结果表明,与野生型相比,突变体叶绿体数目变少,形... 详细信息

以快中子辐射诱变玉米自交系PH6WC筛选得到的碳水化合物分配缺陷突变体ygl3为材料,通过叶片内叶绿素含量和碳水化合物含量的测定、叶绿体结构显微观察、光合特性和遗传特性等进行分析。结果表明,与野生型相比,突变体叶绿体数目变少,形态松散,维管束鞘叶绿体积累了大量的淀粉,淀粉颗粒排列不规则且体积增大,导致了叶绿体的膨胀和膜结构的破坏;叶片中淀粉、葡萄糖、果糖的含量都显著高于野生型,表现出玉米碳水化合物分配缺陷的典型表型特征;突变体的叶绿素a、叶绿素b及总叶绿素含量都显著低于野生型;净光合速率(Photo)、气孔导度(Cond)、蒸腾速率(Trmmol)均极显著低于野生型,胞间二氧化碳浓度(Ci)高于野生型。遗传分析表明,(ygl3×Mo17)F_(2)分离群体突变型(黄化)与野生型(绿色)植株性状分离符合1∶3分离比例,表明该突变体受单个核隐性基因控制。

关键词：玉米碳水化合物突变体ygl3 光合特性遗传分析

具有POLE致病性突变子宫内膜癌的临床病理及分子特征分析

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诊断病理学杂志 2024年第4期31卷 282-286,322页

作者：蔡宇梦周杨汪昊庞钧译吴焕文师晓华梁智勇中国医学科学院北京协和医学院北京协和医院病理科北京100730

目的探讨具有POLE致病性突变子宫内膜癌(EC)的临床病理及分子特征。方法选取179例EC患者,根据POLE基因突变、错配修复(MMR)蛋白表达及p53表达/TP53突变进行分子分型,分析POLE mut型EC的临床病理及分子特征。结果POLE mut20例,MMRd 48例,... 详细信息

目的探讨具有POLE致病性突变子宫内膜癌(EC)的临床病理及分子特征。方法选取179例EC患者,根据POLE基因突变、错配修复(MMR)蛋白表达及p53表达/TP53突变进行分子分型,分析POLE mut型EC的临床病理及分子特征。结果POLE mut20例,MMRd 48例,NSMP 90例,p53 abn 21例。POLE mut EC多为FIGOⅠ期(17/20),中-低分化子宫内膜样癌(EEC)(13/20)、局限于内膜/浅肌层(17/20)、肿瘤浸润性淋巴细胞(TILs)显著(17/20)、无/局灶(LVSI)(18/20)、无淋巴结转移(19/20)。4个亚组间临床病理特征的差异均有统计学意义(P<0.05)。POLE mut型预后最好,具有多重分类特征的病例预后更接近POLE mut型。结论POLE mut EC提示着较高的组织学分级,较早的临床分期及较好的预后;POLE mut可继发dMMR/MSI-H表型及p53/TP53异常,应归类为POLE mut型。

关键词：子宫内膜癌分子分型 POLE 错配修复缺陷 TP53

Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS

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Chinese Journal of Natural Medicines 2021年第6期19卷 473-480页

作者： WEI wen-Long WU Shi-Fei LI Hao-Jv LI Zhen-Wei QU Hua YAO Chang-liang ZHANG Jian-Qing LI Jia-Yuan WU Wan-Ying GUO De-An Shanghai Research Center for Modernization of Traditional Chinese Medicine National Engineering Laboratory for TCM Standardization TechnologyShanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China

Huashi Baidu prescription(HSBDF),recommended in the Guideline for the Diagnosis and Treatment of Novel Coronavirus(2019-nCoV)Pneumonia(On Trials,the Seventh Edition),was clinically used to treat severe corona virus disease 2019(COVID-19)with cough,blood-stained sputum,inhibited defecation,red tongue *** study was aimed to elucidate and profile the knowledge on its chemical constituents and the potential anti-inflammatory effect in *** the study,the chemical constituents in extract of HSBDF were characterized by UPLC-Q-TOF/MS in both negative and positive modes,and the pro-inﬂammatory cytokines were measured by enzyme-linked immunosorbent assays(ELISA)to determine the effects of HSBDF in lipopolysaccharide(LPS)-stimulated RAW264.7 *** results showed that a total of 217 chemical constituents were tentativedly characterized in ***,HSBDF could alleviate the expression levels of IL-6 and TNF-αin the cell models,indicating that the antiviral effects of HSBDF might be associated with regulation of the inflammatory cytokines production in RAW264.7 *** hope that the results could be served as the basic data for further study of HSBDF on anti-COVID-19 effect.

关键词： Huashi Baidu prescription Corona virus disease 2019 Characterization of chemical constituents ELISA

基于迭代中心差分卡尔曼滤波的说话人跟踪方法

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电子与信息学报 2008年第7期30卷 1684-1689页

作者：侯代文殷福亮大连理工大学电子与信息工程学院

利用状态空间方法对说话人进行语音跟踪时,观测方程的非线性会影响说话人位置的估计精度。该文将迭代滤波理论与中心差分卡尔曼滤波技术相结合,提出迭代的中心差分卡尔曼滤波方法,并应用于说话人跟踪系统。仿真实验结果表明,该文所提出... 详细信息

利用状态空间方法对说话人进行语音跟踪时,观测方程的非线性会影响说话人位置的估计精度。该文将迭代滤波理论与中心差分卡尔曼滤波技术相结合,提出迭代的中心差分卡尔曼滤波方法,并应用于说话人跟踪系统。仿真实验结果表明,该文所提出的方法减少了系统线性化误差,增强了滤波算法的鲁棒性,提高了说话人跟踪精度。

关键词：说话人跟踪中心差分卡尔曼滤波麦克风阵列 Levenberg-Marquardt方法

TRIM35 mediates protection against influenza infection by activating TRAF3 and degrading viral PB2

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Protein & Cell 2020年第12期11卷 894-914页

作者： Nan Sun Li Jiang Miaomiao Ye Yihan Wang Guangwen Wang Xiaopeng Wan Yuhui Zhao Xia wen Libin liang Shujie Ma Liling Liu Zhigao Bu Hualan Chen Chengjun Li State Key Laboratory of Veterinary Biotechnology Harbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China

Tripartite motif(TRIM)family proteins are important effectors of innate immunity against viral *** we identified TRIM35 as a regulator of TRAF3 *** in or inhibition of TRIM35 suppressed the production of type I interferon(IFN)in response to viral infection.777m35-deficient mice were more susceptible to influenza A virus(IAV)infection than were wild-type ***35 promoted the RIG-Imediated signaling by catalyzing Lys63-linked polyubiquitination of TRAF3 and the subsequent formation of a signaling complex with VISA and *** PB2 polymerase countered the innate antiviral immune response by impeding the Lys63-linked polyubiquitination and activation of ***35 mediated Lys48-linked polyubiquitination and proteasomal degradation of IAV PB2,thereby antagonizing its suppression of TRAF3 *** in vitro and in vivo findings thus reveal novel roles of TRIM35,through catalyzing Lys63-or Lys48-linked polyubiquitination,in RIG-I antiviral immunity and mechanism of defense against IAV infection.

关键词： influenza A virus PB2 TRIM35 TRAF3 ubiquitination antiviral immunity

适于配送车辆导航路径规划的遍历模型的改进型粒子群优化算法

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华南理工大学学报（自然科学版） 2011年第8期39卷 109-112,117页

作者：章权温惠英孙博长安大学公路学院陕西西安710064 广东省公路管理局广东广州510075 华南理工大学土木与交通学院广东广州510640

车辆路径规划是物流配送导航系统中的关键环节,是实现物流配送路径引导的前提条件和车辆导航的技术保障.为解决物流配送车辆导航中的路径规划问题,文中建立了物流配送车辆导航路径规划(VND)遍历模型,设计了求解该模型的改进型粒子群算法... 详细信息

车辆路径规划是物流配送导航系统中的关键环节,是实现物流配送路径引导的前提条件和车辆导航的技术保障.为解决物流配送车辆导航中的路径规划问题,文中建立了物流配送车辆导航路径规划(VND)遍历模型,设计了求解该模型的改进型粒子群算法,并对初始种群的产生方法及种群的进化策略进行改进,使原本不能直接用于求解VND模型的基本粒子群优化(PSO)算法,在求解VND问题上取得了很好的效果.通过简单算例验证模型和算法的结果表明,该算法具有快速的运算能力和较好的收敛性.

关键词：配送车辆导航路径规划遍历模型粒子群优化算法种群进化

A Novel BIST Approach for Testing Input/Output Buffers in SoCs

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Journal of Electronic Science and Technology of China 2009年第4期7卷 322-325页

作者： Lei Chen Zhi-Ping wen Zhi-quan Zhang Min Wang Beijing Microelectronics Technology Institution Beijing 100076 China

A novel built-in self-test （BIST） approach to test the configurable input/output buffers in Xilinx Virtex series SoCs （system on a chip） using hard macro has been proposed in this paper. The proposed approach can completely detect single and multiple stuck-at gate-level faults as well as associated routing resources in I/O buffers. The proposed BIST architecture has been implemented and verified on Xilinx Virtex series FPGAs （field programmable gate configurations are required array）. Only total of 10 to completely test the I/O buffers of Virtex devices.

关键词： Built-in self-test FPGA I/O buffers SoCs testing.

Identification of Heterodera schachtii on sugar beet in Xinjiang Uygur Autonomous Region of China

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Journal of Integrative Agriculture 2022年第6期21卷 1694-1702页

作者： PENG Huan LIU Hui GAO Li JIANG Ru LI Guang-kuo GAO Hai-feng WU Wei WANG Jun ZHANG Yu HUANG wen-kun KONG Ling-an PENG De-liang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193P.R.China The National Agro-Tech Extension and Service Center Beijing 100026P.R.China Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis of Ministry of Agriculture and Rural Affairs Institute of Plant ProtectionXinjiang Academy of Agricultural SciencesUrumqi 830091P.R.China Agricultural Technology Extension Service Center of Xinyuan County Yining 835800P.R.China Plant Protection Station of Xinjiang Uygur Autonomous Region Urumqi 830006P.R.China

The sugar beet cyst nematode,Heterodera schachtii,is a major parasite of sugar beet which has been recognized and listed as a quarantine nematode in China and more than 20 countries and regions worldwide.A survey for important nematodes was undertaken in the sugar beet planting area of China during 2015-2018,and numerous cysts were collected from sugar beet fields in Xinyuan County,Xinjiang Uygur Autonomous Region of *** observations of morphological and morphometric characteristics revealed that cysts,vulval cones and second-stage juveniles of the Xinjiang population were in the same range of each other and within those of other reported *** *** analysis of rDNA-ITS,28S-D2/D3 and mtDNA cytochrome c oxidase subunit 1(COI)gene sequences suggested that the Xinjiang population clustered in a branch with those foreign populations,and the sequence similarity was as high as 99.81-100%.Moreover,this result was confirmed by PCR assay with species-specific primer SHF6 and rDNA2 of ***,and the pathogenicity test confirmed successful Xinjiang population reproduction in both plant *** conclusion,based on morphological and molecular characterization,this study confirmed that the cyst nematode population collected from sugar beet fields in Xinjiang is *** far as we know,this is the first report of *** on sugar beets in Xinjiang,China.

关键词： sugar beet cyst nematode Heterodera schachtii morphological molecular identification

Molecular phylogeny and inflorescence evolution of Prunus(Rosaceae)based on RAD-seq and genome skimming analyses

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Plant Diversity 2023年第4期45卷 397-408页

作者： Na Su Richard G.J.Hodel Xi Wang Jun-Ru Wang Si-Yu Xie Chao-Xia Gui Ling Zhang Zhao-Yang Chang liang Zhao Daniel Potter Jun wen College of Life Sciences Northwest A&F UniversityYangling 712100China Herbarium of Northwest A&F University Yangling 712100China Department of Botany National Museum of Natural HistoryMRC 166Smithsonian InstitutionWashingtonDC 20013-7012USA College of Life Sciences Tarim UniversityAlaer 843300China Department of Plant Sciences MS2University of CaliforniaDavisCA 95616USA

Prunus is an economically important genus widely distributed in the temperate Northern *** studies on the genus using a variety of loci yielded conflicting phylogenetic ***,we generated nuclear reduced representation sequencing data and plastid genomes for 36 Prunus individuals and two *** nuclear and plastome data recovered a well-resolved *** species were divided into three main clades corresponding to their inflorescence types,-the racemose group,the solitary-flower group and the corymbose group-with the latter two sister to one *** was inferred to have diversified initially in the Late Cretaceous around 67.32 million years *** diversification of the three major clades began between the Paleocene and Miocene,suggesting that paleoclimatic events were an important driving force for Prunus *** state reconstructions revealed that the most recent common ancestor of Prunus had racemose inflorescences,and the solitary-flower and corymb inflorescence types were derived by reduction of flower number and suppression of the rachis,*** also tested the hybrid origin hypothesis of the racemose group proposed in previous *** has undergone extensive hybridization events,although it is difficult to identify conclusively specific instances of hybridization when using SNP data,especially deep in the *** study provides well-resolved nuclear and plastid phylogenies of Prunus,reveals substantial cytonuclear discord at shallow scales,and sheds new light on inflorescence evolution in this economically important lineage.

关键词： Prunus Rosaceae RAD-Seq Chloroplast genome Hybridization Inflorescence evolution