TRIM35 mediates protection against influenza infection by activating TRAF3 and degrading viral PB2

作     者：Nan Sun Li Jiang Miaomiao Ye Yihan Wang Guangwen Wang Xiaopeng Wan Yuhui Zhao Xia Wen Libin Liang Shujie Ma Liling Liu Zhigao Bu Hualan Chen Chengjun Li Nan Sun;Li Jiang;Miaomiao Ye;Yihan Wang;Guangwen Wang;Xiaopeng Wan;Yuhui Zhao;Xia Wen;Libin Liang;Shujie Ma;Liling Liu;Zhigao Bu;Hualan Chen;Chengjun Li

作者机构：State Key Laboratory of Veterinary BiotechnologyHarbin Veterinary Research InstituteChinese Academy of Agricultural SciencesHarbin 150069China 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2020年第11卷第12期

页      面：894-914页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100401[医学-流行病与卫生统计学] 10[医学] 

基　　金：The work was supported by the National Key Research and Development Program of China(2016YFD0500205) the National Natural Science Foundation of China(NSFC)(Grant Nos.31521005,31672582,31422054,and 31472215) the Natural Science Foundation of Heilongjiang Province(JQ2019C005) by the Central Public-Interest Scientific Institution Basal Research Fund(No.Y2017JC35) 

主　　题：influenza A virus PB2 TRIM35 TRAF3 ubiquitination antiviral immunity 

摘      要：Tripartite motif(TRIM)family proteins are important effectors of innate immunity against viral *** we identified TRIM35 as a regulator of TRAF3 *** in or inhibition of TRIM35 suppressed the production of type I interferon(IFN)in response to viral infection.777m35-deficient mice were more susceptible to influenza A virus(IAV)infection than were wild-type ***35 promoted the RIG-Imediated signaling by catalyzing Lys63-linked polyubiquitination of TRAF3 and the subsequent formation of a signaling complex with VISA and *** PB2 polymerase countered the innate antiviral immune response by impeding the Lys63-linked polyubiquitination and activation of ***35 mediated Lys48-linked polyubiquitination and proteasomal degradation of IAV PB2,thereby antagonizing its suppression of TRAF3 *** in vitro and in vivo findings thus reveal novel roles of TRIM35,through catalyzing Lys63-or Lys48-linked polyubiquitination,in RIG-I antiviral immunity and mechanism of defense against IAV infection.