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Metabolic Response of Pakchoi Leaves to Amino Acid Nitrogen

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Journal of Integrative Agriculture 2014年第4期13卷 778-788页

作者： WANG Xiao-li YU wen-juan ZHOU Qian HAN Rui-feng huang Dan-feng Department of Horticulture School of Agriculture and BiologyShanghai Jiao Tong University Instrumental Analysis Center Shanghai Jiao Tong University

Different nitrogen （N） forms may cause changes in the metabolic profiles of plants. However, few studies have been conducted on the effects of amino acid-N on plant metabolic profiles. The main objective of this study was to identify primary metabolites associated with amino acid-N （Gly, Gln and Ala） through metabolic profile analysis using gas chromatography- mass spectrometry （GC-MS）. Plants of pakchoi （Brassica campestris L. ssp. chinensis L.）, Huawang and Wuyueman cultivars, were grown with different nitrogen forms （i.e., Gly, Gln, Ala, NO3-N, and N starvation） applied under sterile hydroponic conditions. The fresh weight and plant N accumulation of Huawang were greater than those of Wuyueman, which indicates that the former exhibited better N-use efficiency than the latter. The physiological performances of the applied N forms were generally in the order of NO3-N〉Gln〉Gly〉Ala. The metabolic analysis of leaf polar extracts revealed 30 amino acid N-responsive metabolites in the two pakchoi cultivars, mainly consisting of sugars, amino acids, and organic acids. Changes in the carbon metabolism of pakchoi leaves under amino acid treatments occurred via the accumulation of fructose, glucose, xylose, and arabinose. Disruption of amino acid metabolism resulted in accumulation of endogenous Gly in Gly treatment, Pro in Ala treatment, and Asn in three amino acid （Gly, Gln and Ala） treatments. By contrast, the levels of endogenous Gln and Leu decreased. However, this reduction varied among cultivars and amino acid types. Amino acid-N supply also affected the citric acid cycle, namely, the second stage of respiration, where leaves in Gly, Gln and Ala treatments contained low levels of malic, citric and succinic acids compared with leaves in NO3-N treatments. No significant difference in the metabolic responses was observed between the two cultivars which differed in their capability to use N. The response of primary metabolites in pakchoi leaves to amino acid-N supply may

关键词： amino acid metabolite profile pakchoi

Identifying the dynamic behaviors in complete reconstruction of Co-based complex precatalysts during electrocatalytic oxygen evolution

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Journal of Energy Chemistry 2025年第1期100卷 226-233页

作者： Jingfang Zhang Danyang Wu Linke Cai Youluan Lu Fanpeng Cheng Lijuan Shi Qun Yi Yao Liu Yi huang Key Laboratory of Green Chemical Engineering Process of Ministry of Education Engineering Research Center of Phosphorus Resources Development and Utilization of Ministry of Education Hubei Key Laboratory of Novel Reactor and Green Chemical Technology School of Chemical Engineering & Pharmacy Wuhan Institute of Technology Key Laboratory of Pesticide & Chemical Biology of Ministry of Education Engineering Research Center of Photoenergy Utilization for Pollution Control and Carbon Reduction of Ministry of Education College of Chemistry Central China Normal University College of Materials Science and Engineering Fuzhou University

Transition metal-based nanomaterials have emerged as promising electrocatalysts for oxygen evolution reaction（OER）. Considerable research efforts have shown that self-reconstruction occurs on these nanomaterials under operating conditions of OER process. However, most of them undergo incomplete reconstruction with limited thickness of reconstruction layer, leading to low component utilization and arduous exploration of real catalytic mechanism. Herein, we identify the dynamic behaviors in complete reconstruction of Co-based complexes during OER. The hollow phytic acid（PA） cross-linked CoFe-based complex nanoboxes with porous nanowalls are designed because of their good electrolyte penetration and mass transport ability, in favor of the fast and complete reconstruction. A series of experiment characterizations demonstrate that the reconstruction process includes the fast substitution of PA by OH-to form Co（Fe）(OH)xand subsequent potential-driven oxidation to Co（Fe）OOH. The obtained CoFeOOH delivers a low overpotential of 290 mV at a current density of 10 mA cm-2and a long-term *** experiment results together with theory calculations reveal that the Fe incorporation can result in the electron rearrangement of reconstructed CoFeOOH and optimization of their electronic structure,accounting for the enhanced OER activity. The work provides new insights into complete reconstruction of metal-based complexes during OER and offers guidelines for rational design of high-performance electrocatalysts.

关键词： Oxygen evolution reaction

Effect of Ti Additions on Mechanical and Thermodynamic Properties of W-Ti Alloys: A First-principles Study

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Journal of Wuhan University of Technology(Materials Science) 2025年第1期40卷 246-257页

作者： ZHANG Jian NIE Wei huang Jin ZHU Ke LIU Ruxia ZHANG Ruizhi LUO Guoqiang SHEN Qiang State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070China Institute for Carbon Neutrality University of Science and Technology BeijingBeijing 100083China

The mechanical and thermodynamic properties of W-Ti alloys(including W_(15)Ti_(1),W_(14)Ti_(2),W_(12)Ti_(4) and W_(8)Ti_(8) alloys)were investigated by the first-principles approach based on density functional *** results indicate that W-Ti alloys except W_(8)Ti_(8) are thermodynamically *** modulus and hardness of W-Ti alloys are smaller than those of pure tungsten and gradually decrease with increasing Ti ***,their B/G ratios and Poisson's ratios exceed those of pure tungsten,suggesting that the introduction of Ti decreases the mechanical strength while enhancing the ductility of W-Ti *** thermal expansion coefficients for W-Ti alloys all surpass those of pure tungsten,indicating that the introduction of titanium exacerbates the thermal expansion behavior of W-Ti ***,elevated pressure has the capacity to suppress the thermal expansion tendencies in titanium-doped tungsten *** study offers theoretical insights for the design of nuclear materials by exploring the mechanical and thermodynamic properties of W-Ti alloys.

关键词： first-principles mechanical properties thermodynamic properties tungsten-titanium alloys

Ωω States in a Chiral Quark Model

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Communications in Theoretical Physics 2007年第4X期48卷 695-698页

作者： WANG wen-Ling huang Fei ZHANG Zong-Ye YU You-wen LIU feng Institute of Particle Physics Huazhong Normal University Wuhan 430079 China CCAST (World Laboratory) P.O. Box 8730 Beijing 100080 China Institute of High Energy Physics P.O. Box 918-4 Beijing 100049 China

The structures of Ωω states with spin-parity JP= 5/2^-, 3/2^-, and 1/2^- are dynamically studied in both the chlral SU（3） quark model and the extended chiral SU（3） quark model by solving a resonating group method （RGM） equation. The model parameters are taken from our previous work, which gave a satisfactory description of the energies of the baryon ground states, the binding energy of the deuteron, the nucleon-nucleon （NN） scattering phase shifts, and the hyperon-nucleon （YN） cross sections. The calculated results show that the Ωω state has an attractive interaction, and in the extended chiral SU（3） quark model such attraction can make for a Ωω quasi-bound state with spin-parity JP = 3/2^- or 5/2^- and the binding energy of about several MeV.

关键词： Ωω quasi-bound states quark model chiral symmetry

基于轻量化卷积神经网络的桥梁斜拉索PE护套损伤识别方法

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土木与环境工程学报（中英文） 2025年第1期47卷 167-178页

作者：刘啸宇黄永徐峰李惠上海交通大学船舶海洋与建筑工程学院上海200240 哈尔滨工业大学土木工程学院哈尔滨150090

深度神经网络和计算机视觉技术近年来在结构健康监测中发挥了越来越重要的作用。利用无人机航拍采集的桥梁斜拉索损伤图像数据,研究基于深度学习技术的斜拉索PE护套损伤识别方法。为实现在较低运算能力设备上对大跨度桥梁斜拉索表面局... 详细信息

深度神经网络和计算机视觉技术近年来在结构健康监测中发挥了越来越重要的作用。利用无人机航拍采集的桥梁斜拉索损伤图像数据,研究基于深度学习技术的斜拉索PE护套损伤识别方法。为实现在较低运算能力设备上对大跨度桥梁斜拉索表面局部损伤的智能快速识别,解决传统深度卷积神经网络的运算效率相对较低、模型参数规模较大的问题,提出轻量化处理的区域推荐型卷积神经网络模型。介绍区域推荐网络与其轻量化改进方法的理论基础,分析轻量化模型处理的必要性,其能在保证识别精度的前提下降低模型训练与预测的设备性能需求,达到节约计算资源与时间的目的;通过数据增广等多手段解决损伤样本数据量不足的问题,设置对比试验,统计分析结果,验证了轻量化神经网络模型的优越性。结果表明,轻量化网络在牺牲少量识别准确度的前提下,能够在较大程度上实现对模型复杂度与计算量的改进,在工程应用中能有效拓展神经网络的实用性。

关键词：桥梁斜拉索智能损伤识别轻量化神经网络计算机视觉深度学习

Vasculature-on-a-chip with stretchable sensor for recapitulating hemodynamics and electrochemical monitoring of endothelium

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Science China Chemistry 2023年第11期66卷 3314-3322页

作者： wen-Ting Fan Yi Zhao feng Hong Yan-Ling Liu Wei-Hua huang College of Chemistry and Molecular Sciences Wuhan UniversityWuhan 430072China

Vascular endothelium can perceive fluid shear stress(FSS)and cyclic circumferential stretch(CCS)caused by the pulsatile blood flow,and translate the hemodynamics into biochemical signals to regulate vascular ***,existing methods provide little information about the real-time biochemical responses of endothelium when exposed to dynamic FSS and ***,a vasculature-on-a-chip integrated with stretchable sensing is engineered for recapitulating the hemodynamic milieus and in-situ monitoring biochemical responses of endothelial *** integrated device is developed by sandwiching a robust stretchable electrode between an upper fluidic channel and a lower pneumatic *** fluidic and pneumatic channels enable the simultaneous recapitulation of both FSS and CCS,and the integrated sensor exhibits excellent cell-adhesive capacity and electrochemical sensing stability even after long-term hemodynamic *** allow real-time monitoring of hemodynamic form-and duration-dependent endothelium responses,and further efficacy investigation about a recommended drug for COVID-19,demonstrating the great potential in vascular disease and drug screening.

关键词： vasculature-on-a-chip stretchable sensor hemodynamic forces electrochemical monitoring endothelium responses

Synthesis and Biological Activity of New Pyrethroid Acid Oxime-esters Containing Pyrazole Ring

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Chemical Research in Chinese Universities 2003年第3期19卷 297-301页

作者： MA Jun-an huang Run-qiu feng Lei SONG Jian QIU De-wen Institute and State Key Laboratory of Elemento-organic Chemistry Nankai UniversityTianjin 300071P.R.China

A series of compounds containing oxime-ester linkage and pyrazole ring(in place of the ester linkage and the alcohol moiety in pyrethroid ester) was designed and synthesized. The structures of all the compounds prepared were confirmed by 1H NMR and MS spectroscopy as well as elemental analyses. The bioassay data of those compounds against tobacco mosaic virus(TMV), cucumber mosaic virus(CMV), potato virus X(PVX) and potato virus Y(PVY) were presented. Among them compound 6i was found to possess significant plant antiviral activities. But all the compounds showed low insecticidal and acaricidal activities.

关键词： Pyrethroid Oxime-ester Synthesis Pyrazole Plant antiviral activity

Statistical extremes and peak factors in wind-induced vibration of tall buildings

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2012年第1期13卷 18-32页

作者： Ming-feng huang Chun-man CHAN wen-juan LOU Kenny Chung-Siu KWOK Institute of Structural Engineering Zhejiang University Hangzhou 310058 China Department of Civil and Environmental Engineering Hong Kong University of Science and Technology Hong Kong School of Engineering University of Western Sydney NSW Australia

In the structural design of tall buildings, peak factors have been widely used to predict mean extreme responses of tall buildings under wind excitations. Vanmarcke＇s peak factor is directly related to an explicit measure of structural reliability against a Gaussian response process. We review the use of this factor for time-variant reliability design by comparing it to the conven- tional Davenport＇s peak factor. Based on the asymptotic theory of statistical extremes, a new closed-form peak factor, the so-called Gamma peak factor, can be obtained for a non-Gaussian resultant response characterized by a Rayleigh distribution process. Using the Gamma peak factor, a combined peak factor method was developed for predicting the expected maximum resultant responses of a building undergoing lateral-torsional vibration. The effects of the standard deviation ratio of two sway components and the inter-component correlation on the evaluation of peak resultant response were also investigated. Utilizing wind tunnel data derived from synchronous multi-pressure measurements, we carried out a wind-induced time history response analysis of the Common- wealth Advisory Aeronautical Research Council （CAARC） standard tall building to validate the applicability of the Gamma peak factor to the prediction of the peak resultant acceleration. Results from the building example indicated that the use of the Gamma peak factor enables accurate predictions to be made of the mean extreme resultant acceleration responses for dynamic service- ability performance design of modem tall buildings.

关键词： Level-crossing rate (LCR) Wind-induced vibration Mean extreme response Combined resultant process Peakfactor method

Comprehensive Suitability Evaluation of Tea Crops Using GIS and a Modified Land Ecological Suitability Evaluation Model

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Pedosphere 2012年第1期22卷 122-130页

作者： LI Bo ZHANG feng ZHANG Li-wen huang Jing-feng JIN Zhi-feng D.K.GUPTA College of Public Administration Zhejiang UniversityHangzhou 310058(China) Institute of Agricultural Remote Sensing and Information Technology Zhejiang UniversityHangzhou 310058(China) Zhejiang Climatic Centre Zhejiang Meteorological BureauHangzhou 310017(China) Departamet of Biochemistry Molecular Cell Biology of Plant Experimental InstitutionGranada(Spain)

Tea （Camellia sinensis） is one of the most valuable cash crops in southern China; however, the planting distribution of tea crops is not optimal and the production and cultivation regions of tea crops are restricted by law and custom. In order to evaluate the suitability of tea crops in Zhejiang Province, the annual mean temperature, the annual accumulated temperature above 10 ℃, the frequency of extremely low temperature below -13 ℃, the mean humidity from April to October, slope, aspect, altitude, soil type, and soil texture were selected from climate, topography, and soil factors as factors for land ecological evaluation by the Delphi method based on the ecological characteristics of tea crops. These nine factors were quantitatively analyzed using a geographic information system （GIS）. The grey relational analysis （GRA） was combined with the analytic hierarchy process （AHP） to address the uncertainties during the process of evaluating the traditional land ecological suitability, and a modified land ecological suitability evaluation （LESE） model was built. Based on the land-use map of Zhejiang Province, the regions that were completely unsuitable for tea cultivation in the province were eliminated and then the spatial distribution of the ecological suitability of tea crops was generated using the modified LESE model and GIS. The results demonstrated that the highly, moderately, and non-suitable regions for the cultivation of tea crops in Zhejiang Province were 27552.66, 42 724.64, and 26507.97 km2, and accounted for 28.47%, 44.14%, and 27.39% of the total evaluation area, respectively. Validation of the method showed a high degree of coincidence with the current planting distribution of tea crops in Zhejiang Province. The modified LESE model combined with GIS could be useful in quickly and accurately evaluating the land ecological suitability of tea crops, providing a scientific basis for the rational distribution of tea crops and acting as a reference to land pol

关键词： analytic hierarchy process (AHP) grey relational analysis (GRA) soil temperature topography