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CD137 costimulation enhances the antiviral activity of Vγ9Vδ2-T cells against influenza virus

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Signal Transduction and Targeted Therapy 2020年第1期5卷 1746-1755页

作者： Yujun Pei Kun wen Zheng Xiang Chunyu Huang Xiwei Wang Xiaofeng Mu liyan wen Yinping Liu wenwei Tu Department of Paediatrics and Adolescent Medicine Li Ka Shing Faculty of MedicineThe University of Hong KongHong KongChina

Influenza epidemics and pandemics are constant threats to global public *** strategies including vaccines and antiviral drugs have achieved great advances in controlling influenza virus infection,the efficacy of these strategies is limited by the highly frequent mutations in the viral genome and the emergence of drug-resistant *** previous study indicated that boosting the immunity of human Vγ9Vδ2-T cells with the phosphoantigen pamidronate could be a therapeutic strategy to treat seasonal and avian influenza virus ***,one notable drawback ofγδ-T cell-based immunotherapy is the rapid exhaustion of proliferation and effector responses due to repeated treatments with ***,we found that the expression of CD137 was inducible in Vγ9Vδ2-T cells following antigenic ***137^(+)Vγ9Vδ2-T cells displayed more potent antiviral activity against influenza virus than their CD137−counterparts in vitro and in Rag2^(-/-)γc^(-/-)*** further demonstrated that CD137 costimulation was essential for Vγ9Vδ2-T cell activation,proliferation,survival and effector *** humanized mice reconstituted with human peripheral blood mononuclear cells,CD137 costimulation with a recombinant human CD137L protein boosted the therapeutic effects of pamidronate against influenza *** study provides a novel strategy of targeting CD137 to improve the efficacy of Vγ9Vδ2-T cell-based immunotherapy.

关键词： CD137 influenza drugs

Uncompromised NK cell activation is essential for virus-specific CTL activity during acute influenza virus infection

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Cellular & Molecular Immunology 2018年第9期15卷 827-837页

作者： Yuan Liu Jian Zheng Yinping Liu liyan wen Lei Huang Zheng Xiang Kwok-Tai Lam Aizhen Lv Huawei Mao Yu-Lung Lau wenwei Tu Department of Pediatrics and Adolescent Medicine Li Ka Shing Faculty of MedicineThe University of Hong KongHong KongChina Medical Genetic Center Guangdong Women and Children HospitalGuangzhou 511400China Department of Radiology Georgia Regents UniversityAugustaGA 30912USA GRU Cancer Center Georgia Regents UniversityAugustaGA 30912USA Department of Pediatrics The University of Hong Kong—Shenzhen HospitalShenzhen 518053China

Natural killer (NK) cells are indispensable components of both the innate and adaptive immune response. However,their precise roles in the cross-talk between innate and adaptive immunity during influenza virus infection remaincontroversial. By comparing NK cell dynamics and activity under a sub-lethal dose and high dose of influenza virusinfection, we showed that influenza virus PR8 directly infected NK cells during natural infection, which wasconsistent with our previous findings obtained from an in vitro investigation of human NK cells. The impairments incytotoxicity and IFN-γ production by spleen NK cells following high-dose infection were accompanied by decreasedvirus-specific killing mediated by cytotoxic T lymphocytes (CTLs). Importantly, the weakened CTL activity could bereversed by adoptive transfer of spleen NK cells harvested from low-dose-infected mice but not healthy *** together, our data provide direct evidence supporting the contribution of NK cells to antiviral T-cellresponses. This study also indicates that a novel NK-targeted immune evasion strategy is used by influenza virusto shrink both innate and adaptive immune responses.

关键词： CTL influenza NK cell

Detection of herpes simplex virus type 1 in rheumatic valvular tissue

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Chinese Medical Journal 2005年第5期118卷 370-376页

作者： PANZhi-gang WANGXiu-nan liyan-wen ZHANGHong-yi LeonardC.Archard DivisionofGeneralPractice ZhongshanHospitalandDepartmentofGeneralPracticeShanghaiMedicalCollegeFudanUniversityShanghai200032China DepartmentofPathology ZhongshanHospitalFudanUniversityShanghai200032China DivisionofBiomedicalSciences ImperialCollegeFacultyofMedicineLondonSW72AZU.K.

Background Rheumatic heart disease (RHD) is the most important sequela of rheumatic fever (RF): evidence that streptococcal infection is aetiological is prominent, but sometimes contradictory. Acute HSV-1 infection in mouse leads to carditis and valvulitis whereas recurrent infection results in inflammatory granulomatous lesions that resemble Aschoff bodies. Cells containing HSV-1 inclusions or virus infected giant cells appear similar to Anitschkow cells or Aschoff cells respectively. We hypothesized that HSV-1 infection also may be involved in RHD. Methods Formalin-fixed, paraffin-embedded valvular tissue samples from 32 patients with RHD were investigated for evidence of HSV-1 infection. HSV-1 antigen was detected by immunohistochemistry, using HSV-1-specific monoclonal and polyclonal antibodies. HSV-1 glycoprotein D gene sequences were amplified by nPCR, using β-globin gene amplification in the same samples as internal control. Valvular tissue from 5 cases of sudden death and 3 cases died of neisseria meningitis without a history of valvular disease was used for comparison. HSV-1-infected lung tissue was used as positive control. Results HSV-1 antigens were detected in valvular tissues from 21 of 32 (65.6%) patients. Fifteen of these 21 (46.9% of cases), but no antigen-negative sample, were positive also for HSV DNA. Nucleotide sequence of PCR products was homologous to the targeted region of the HSV-1 glycoprotein D gene. HSV-1 antigen was present also in one case of sudden death but viral DNA was not found in any tissue sample from the comparison group. Results from reagent and positive controls were as *** This is the first study to show the presence of HSV-1 antigen and genomic DNA in valvular tissues from patients with RHD and provides evidence for an association of HSV-1 infection with some cases of rheumatic valvular disease.

关键词： rheumatic heart disease · herpes simplex virus type 1 · immunohistochemistry · polymerase chain reaction

Dynamic Modeling and Flight Simulation of a Folding Wing-Tip UAV

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Dynamic Modeling and Flight Simulation of a Folding Wing-Tip...

对称折叠翼尖变体飞行器的纵向建模与气动分析

第32届中国控制与决策会议

作者： Jie Yang liyan wen Bin Jiang Zhaoyu Wang Department of Automation Engineering Nanjing University of Aeronautics and Astronautics Jiangsu Key Laboratory of Internet of Things and Control Technologies

In this paper,a novel modeling method and the aerodynamic analysis of folding wing-tip UAVs are *** folding wing-tip UAV models are established by treating the aircraft as a single body but relaxing the condition of *** a typical kind of folding wing-tip UAVs,NASA spanwise adaptive wing morphing UAV（SAW） is modeled and analyzed in this *** missile datcom is also applied to analyze the aerodynamic performance during the folding process of wing-tip by establishing the 3 D model of the SAW and conducting numeric simulations.

关键词： Morphing Aircraft Aerodynamic Modeling Missile DATCOM

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Transactions of Nanjing University of Aeronautics and Astronautics 2023年第4期40卷 382-400页

作者：文利燕任秋萍杨杰向政超南京航空航天大学自动化学院南京211106 中国船舶工业集团第七〇八研究所民船部电气科上海200011 南京航空航天大学航空学院南京210016

为研究展向自适应机翼的气动特性随翼尖折叠角的变化规律,本文以一种新型形状记忆合金驱动的折叠翼尖变体飞行器-改进型Cessna 550为研究对象,提出了一种基于翼尖折叠角度的可变气动参数的纵向非线性动力学建模方法。首先,为了探索翼尖... 详细信息

为研究展向自适应机翼的气动特性随翼尖折叠角的变化规律,本文以一种新型形状记忆合金驱动的折叠翼尖变体飞行器-改进型Cessna 550为研究对象,提出了一种基于翼尖折叠角度的可变气动参数的纵向非线性动力学建模方法。首先,为了探索翼尖折叠运动如何影响飞行器的气动性能,本文采用计算流体动力学建立了变体飞行器的三维模型,并通过气动仿真获得不同翼尖折叠角度下的气动系数。通过曲线拟合得到了各气动参数关于翼尖折叠角度的函数关系式,建立了关于翼尖折叠角度的变体飞行器非线性动力学模型。进而,基于所得到的气动参数,对飞行器的起飞、机动和着陆3个阶段以及折叠过程中的纵向静稳定性进行了分析,得到了折叠翼尖变体飞行器在不同的飞行状态下的最佳变体策略。最后,通过纵向稳定分析验证了所提变形策略的可行性。

关键词：变体飞行器气动评估动态建模纵向建模对称折叠翼尖

Pipelining Design Controlled by Stepper Motor Based on SCM

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Pipelining Design Controlled by Stepper Motor Based on SCM

切换拓扑下异构多智能体系统自适应输出一致性

2010 Chinese Control and Decision Conference

作者： LingXi Pei,Chen Peng,Jing Guo,liyan wen School of Electrical and Automation Engineering,Nanjing Normal University,Nanjing,Jiangsu,210042

＜正＞By employing electronics and intelligent technology,this paper realizes an effective integration of mechanics and electronics in pipelining packing *** achieve this aim,stepper motor is controlled by a single-chip microcomputer（SCM）type of STC89C516RD+.Once the photoelectric switch type of LJ-JT02 detects products passing, signals are given out to SCM to direct the stepper ***,the scissors are used to cut off the package tape, which are realized by a relay type of *** experimental results show-the effectiveness of above design.

关键词： Single-chip Microcomputer(SCM) Stepper Motor Driving-chip Relay

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上海交通大学学报 2024年第11期58卷 1805-1815,I0001,I0002页

作者：刘宇文利燕姜斌马亚杰崔玉康南京航空航天大学自动化学院南京210016

针对通信拓扑突变的不确定异构多智能体系统,将拓扑突变等效为切换拓扑问题,进而设计一种基于领导者-模型-跟随者匹配的分布式自适应协同控制方法,实现领导者-跟随者输出一致性.首先,提出局部输出跟踪误差概念,将领导者-跟随者输出一致... 详细信息

针对通信拓扑突变的不确定异构多智能体系统,将拓扑突变等效为切换拓扑问题,进而设计一种基于领导者-模型-跟随者匹配的分布式自适应协同控制方法,实现领导者-跟随者输出一致性.首先,提出局部输出跟踪误差概念,将领导者-跟随者输出一致性问题转化为相邻智能体局部输出一致性问题;然后,针对系统参数已知的情况,进行分布式标称协同控制器设计,实现参考模型-领导者匹配和跟随者-参考模型匹配,以保证通信拓扑突变下领导者-跟随者输出一致性;进而,针对系统参数未知的情况,进行分布式自适应协同控制器设计,实现通信拓扑突变下跟随者输出对领导者输出的渐近跟踪.所设计的控制方法不依靠全局信息即可保证所有智能体系统闭环稳定及跟随者对领导者的输出一致性.最后,通过一个仿真案例验证了所设计的控制方案的有效性.

关键词：异构多智能体系统输出一致性自适应控制切换拓扑

Characterization of humoral immune responses against SARS-CoV-2 accessory proteins in infected patients and mouse model

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Virologica Sinica 2024年第3期39卷 414-421页

作者： Yuming Li Yanhong Tang Xiaoqian Wang Airu Zhu Dongdong Liu Yiyun He Hu Guo Jie Zheng Xinzhuo Liu Fengyu Chi Yanqun Wang Zhen Zhuang Zhaoyong Zhang Donglan Liu Zhao Chen Fang Li Wei Ran Kuai Yu Dong Wang liyan wen Jianfen Zhuo Yanjun Zhang Yin Xi Jingxian Zhao Jincun Zhao Jing Sun School of Public Health Shandong First Medical University&Shandong Academy of Medical SciencesJi'nan250117China Key Laboratory of Emerging Infectious Diseases in Universities of Shandong Shandong First Medical University&Shandong Academy of Medical SciencesJi'nan250117China State Key Laboratory of Respiratory Disease National Clinical Research Center for Respiratory DiseaseNational Center for Respiratory MedicineGuangzhou Institute of Respiratory HealthThe First Affiliated Hospital of Guangzhou Medical UniversityGuangzhouGuangdong510182China Hunan Provincial People's Hospital The First Affiliated Hospital of Hunan Normal UniversityHunan Normal UniversityChangsha410005China Guangzhou National Laboratory GuangzhouGuangdong510005China Shanghai Institute for Advanced Immunochemical Studies School of Life Science and TechnologyShanghai Tech UniversityShanghai201210China Institute for Hepatology National Clinical Research Center for Infectious DiseaseShenzhen Third People's HospitalThe Second Affiliated HospitalSchool of MedicineSouthern University of Science and TechnologyShenzhen518005China

Severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),the causative agent of COVID-19,encodes several accessory proteins that have been shown to play crucial roles in regulating the innate immune ***,their expressions in infected cells and immunogenicity in infected humans and mice are still not fully *** study utilized various techniques such as luciferase immunoprecipitation system(LIPS),immunofluorescence assay(IFA),and western blot(WB)to detect accessory protein-specific antibodies in sera of COVID-19 *** antibodies to proteins 3a,3b,7b,8 and 9c can be detected by LIPS,but only protein 3a antibody was detected by IFA or *** against proteins 3a and 7b were only detected in ICU patients,which may serve as a marker for predicting disease ***,we investigated the expression of accessory proteins in SARS-CoV-2-infected cells and identified the expressions of proteins 3a,6,7a,8,and *** also analyzed their ability to induce antibodies in immunized mice and found that only proteins 3a,6,7a,8,9b and 9c were able to induce measurable antibody productions,but these antibodies lacked neutralizing activities and did not protect mice from SARS-CoV-2 *** findings validate the expression of SARS-CoV-2 accessory proteins and elucidate their humoral immune response,providing a basis for protein detection assays and their role in pathogenesis.

关键词： SARS-CoV-2 Accessory protein Humoral immune responses COVID-19 Mouse model

“小小建筑师”职业角色体验中的劳动能力培养

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当代家庭教育 2022年第7期 16-19页

作者：闻丽燕江苏省张家港市北庄幼儿园

幼儿教育的方式与内容均存在独特之处,大班幼儿可用独特方式与周边环境展开互动并从中感悟何为劳动。基于上述背景,教师可引入"小小建筑师"职业角色体验活动,让幼儿能够在活动中体验"建筑师"这一职业角色,逐步引导幼儿具有劳动意识,培... 详细信息

幼儿教育的方式与内容均存在独特之处,大班幼儿可用独特方式与周边环境展开互动并从中感悟何为劳动。基于上述背景,教师可引入"小小建筑师"职业角色体验活动,让幼儿能够在活动中体验"建筑师"这一职业角色,逐步引导幼儿具有劳动意识,培养幼儿的劳动能力、劳动习惯。本文将从多角度探寻结合"小小建筑师"角色体验活动对幼儿的劳动能力进行培养的有效策略,以期强化幼儿的劳动素养。

关键词：职业角色体验小小建筑师幼儿劳动