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Middle Devonian ostracods from Jiwozhai patch reef(Dushan,Guizhou Province,South China):Biostratigraphic and palaeoecological implication

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Journal of Palaeogeography 2024年第3期13卷 581-593页

作者： Jun-Jun Song Jia-Yuan Huang wen Guo kun Liang Yu-Cong Sun Zhi-Hong Wang wen-kun Qie State Key Laboratory of Palaeobiology and Stratigraphy Nanjing Institute of Geology and PalaeontologyChinese Academy of SciencesNanjing 210008Jiangsu ProvinceChina Hubei Key Laboratory of Paleontology and Geological Environment Evolution Wuhan Center of China Geological SurveyWuhan 430205Hubei ProvinceChina University of Chinese Academy of Sciences NanjingNanjing 211135Jiangsu ProvinceChina

The Middle Devonian ostracods from the Jiwozhai patch reef in Dushan, Guizhou Province, South China were described for the first time in this study. Twenty-nine species belonging to 20 genera were identified and figured, including a new species named Wangshangkia jiwozhaiensis sp. nov. The ostracod fauna in the Jiwozhai patch reef represents a Givetian age. The ostracod assemblage belongs to the smooth-podocopid association, which is also ecologically equivalent to the Eifelian Mega-Assemblage Ⅲ. This association is indicative of shallow open-marine environments. The Jiwozhai patch reef fauna is primarily comprised of suspension feeders, including filter-feeding ostracods, corals, brachiopods and bryozoans. Ostracods from the Jiwozhai patch reef were compared to the ostracod faunas from Laurentia-Baltica at generic level, which reveals limited faunal exchanges between South China and Laurentia-Baltica during the Middle Devonian.

关键词： Ostracods Givetian Jiwozhai patch reef Dushan Palaeoecology

A component-based aircraft instrument rapid modeling tool

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Journal of Zhejiang University-Science C(Computers and Electronics) 2010年第11期11卷 911-918页

作者： Fang-wen LI Xu-kun SHEN State Key Lab of Virtual Reality Technology and Systems School of Computer Science Beihang University Beijing 100191 China

Component-based software reuse and development is considered to be the best way to improve the efficiency and quality of *** meet the demands of aircraft instrument exhibition in an aviation virtual library,we present a modeling and simulation tool for cockpit instruments on component methods,achieving display functionality and providing a visual interaction platform in the aviation virtual *** the rapid model construction and reuse of virtual library resources,we classify entities in the cockpit into several categories by their behavior,and design a template for each category to describe its *** efficiently reduces the modeling cycle,and thus largely helps users to take advantage of the library resource.

关键词： Components Rapid modeling Simulation

Coating of microbially produced calcium carbonate onto stone materials

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Science China(Technological Sciences) 2015年第2期58卷 266-272页

作者： ZHU wen kun MU Tao ZHANG You Kui DUAN Tao LUO Xue Gang Engineering Research Center of Biomass Materials Ministry of Education Southwest University of Science and Technology China Academy of Engineering Physics Joint Laboratory for Extreme Conditions Matter Properties Southwest University of Science and Technology and Research Center of Laser FusionCAEP

The coatings of microorganism-induced calcium carbonate onto the stone surface carried out by using both of the immersion method and coating method were investigated. Various analysis and testing techniques such as scanning electron micrograph （SEM） and X-ray diffraction （XRD） were used to characterize the deposited mineral layer. The adhesive property, acid re- sistance, frost resistance, light and aging resistance, water adsorption and permeability were investigated in detail. The results showed that both immersion method and coating method could produce calcium carbonate granules with sizes ranging from 1 to 10 pm and form a layer of dense mineralization membrane which is about 50 to 100 pm thick. Immersion method was more efficient than coating method. The large cohesive force between calcium carbonate layer and stone materials could improve the acid rain resistance as well as excellent heat tolerance, frost resistance and light aging resistance. The coating process could not only help the stone materials maintain its original permeability with the aid of calcium carbonate layers but also improve the penetration resistance significantly. Therefore, this type of technology shows a great potential in the protection of stone relics.

关键词： microorganism microbially induced deposited-layer stone materials

Design and Algorithm Research of a GNSS/FOG-SINS Integrated Navigation System for Unmanned Vehicles 36

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Design and Algorithm Research of a GNSS/FOG-SINS Integrated ...

Molecular Characterization and Functional Analysis of a New Acid Phosphatase Gene(Ha-acp1) from Heterodera avenae

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作者： Han Wang Maosong Wang kun wen wenqi Wu College of Mechatronics Engineering and Automation National University of Defense Technology The University of Calgary

ISBN: (纸本)9781538629185

According to the application requirements of autonomous navigation for unmanned vehicles which mainly used for forest exploration and border patrols,this paper designs a cost effective GNSS/SINS integrated system based on the fiber optic gyro（FOG）.After that,the alignment method of SINS and the integrated navigation algorithm are also *** fusing the valid data of GNSS/FOG-SINS with Kalman filter,the error of inertial devices can be corrected and the high-precision inertial navigation results are used to locate by the satellite navigation *** the end of this paper,the simulation and the vehicle field test are done,from which we can see that the tested accuracy can meet the practical precision requirement of the integrated navigation system when the satellite signal is blocked in the complex terrain environment for one minute.

关键词： Unmanned Vehicles GNSS/FOG-SINS Integrated Navigation Kalman Filter

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Journal of Integrative Agriculture 2014年第6期13卷 1303-1310页

作者： LIU Yan-ke HUANG wen-kun LONG Hai-bo PENG Huan HE wen-ting PENG De-liang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences

For sedentary endo-parasitic nematodes, parasitism genes encoding secretory protein expressed in the subventral glands cells always play an important role during the early parasitic process. A new acid phosphatase gene （Ha-acp1） expressed in the subventral glands of the cereal cyst nematode （Heterodera avenae） was cloned and the characteristics of the gene were analyzed. Results showed that the gene had a putative signal peptide for secretion and in situ hybridization showed that the transcripts of Ha-acp1 accumulated speciifcally in the subventral gland cells of H. avenae. Southern blot analysis suggested that Ha-acp1 belonged to a multigene family. RT-PCR analysis indicated that this transcription was strong at the pre-parasitic juveniles. Knocking down Ha-acp1 using RNA interference technology could reduce nematode infectivity by 50%, and suppress the development of cyst. Results indicated that Ha-acp1 could play an important role in destroying the defense system of host plants.

关键词： Heterodera avenae acid phosphatase RNA interference

Regulation of Microstructure of Calcium Carbonate Crystals by Egg White Protein

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Chemical Research in Chinese Universities 2012年第2期28卷 180-185页

作者： ZHU wen-kun LUO Xue-gang ZHANG Chi DUAN Tao ZHOU Jian School of National Defense Science and Technology Southwest University of Science and TechnologyMianyang 621010P.R.China Engineering Research Center of Biomass Materials of Ministry of Education Southwest University of Science and TechnologyMianyang 621010P.R.China

Crystal growth of calcium carbonate in biological simulation was investigated via egg white protein with different volume fractions,during which calcium carbonate was synthesized by calcium chloride and sodium *** morphology,thermal properties and microstructure of the calcium carbonate micro-to-nanoscale crystals were characterized by scanning electron microscopy（SEM）,transmission electron microscopy（TEM）,Fourier transform infrared spectroscopy（FTIR）,thermogravimetric analysis（TG） and X-ray diffraction（XRD） *** results show that the volume fraction of egg white protein has great influence on the shape,size and morphology of calcium carbonate *** calcium carbonate crystals were the mixtures of calcite-vaterite-like crystals including spherical and rough surface,which are different from that formed in pure *** the increase of egg white protein concentration,the diameter of calcium carbonate crystals changed,the amount of formed spherical calcium carbonate particles decreased and that of vaterite *** results indicate that the coordination and electrostatic interaction between egg white protein and Ca2＋ significantly affect the calcium carbonate crystalization.

关键词： Egg white protein Spherical calcium carbonate Vaterite Calcite

CD137 costimulation enhances the antiviral activity of Vγ9Vδ2-T cells against influenza virus

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Signal Transduction and Targeted Therapy 2020年第1期5卷 1746-1755页

作者： Yujun Pei kun wen Zheng Xiang Chunyu Huang Xiwei Wang Xiaofeng Mu Liyan wen Yinping Liu wenwei Tu Department of Paediatrics and Adolescent Medicine Li Ka Shing Faculty of MedicineThe University of Hong KongHong KongChina

Influenza epidemics and pandemics are constant threats to global public *** strategies including vaccines and antiviral drugs have achieved great advances in controlling influenza virus infection,the efficacy of these strategies is limited by the highly frequent mutations in the viral genome and the emergence of drug-resistant *** previous study indicated that boosting the immunity of human Vγ9Vδ2-T cells with the phosphoantigen pamidronate could be a therapeutic strategy to treat seasonal and avian influenza virus ***,one notable drawback ofγδ-T cell-based immunotherapy is the rapid exhaustion of proliferation and effector responses due to repeated treatments with ***,we found that the expression of CD137 was inducible in Vγ9Vδ2-T cells following antigenic ***137^(+)Vγ9Vδ2-T cells displayed more potent antiviral activity against influenza virus than their CD137−counterparts in vitro and in Rag2^(-/-)γc^(-/-)*** further demonstrated that CD137 costimulation was essential for Vγ9Vδ2-T cell activation,proliferation,survival and effector *** humanized mice reconstituted with human peripheral blood mononuclear cells,CD137 costimulation with a recombinant human CD137L protein boosted the therapeutic effects of pamidronate against influenza *** study provides a novel strategy of targeting CD137 to improve the efficacy of Vγ9Vδ2-T cell-based immunotherapy.

关键词： CD137 influenza drugs

Assaying the potential of twenty-one legume plants in Medicago truncatula and M. sativa for candidate model plants for investigation the interactions with Heterodera glycines

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Journal of Integrative Agriculture 2016年第3期15卷 702-704页

作者： KONG Ling-an WU Du-qing HUANG wen-kun PENG Huan HE wen-ting PENG De-liang State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences

Soybean cyst nematode Heterodera glycines is one of the most serious soil-borne pathogens in soybean production. However, the researches were limited in China due to lack of an effective pathosystem. In this study, we screened 21 legume Medicago plants in both Medicago truncatula and Medicago sativa to obtain candidate model plants for establishing a new pathosystem for legume-H. glycines interactions. The nematode infection of tested plants was assayed with Race 3 and 4 respectively, which were two dominant H. glycines inbred races in China soybean producing areas. The results showed that the model legume plant M. truncatula A17 failed to allow Race 3 of H. glycines to complete its life cycle, in contrast, it provided the Race 4 population to form several cyst nematodes, however, the female index（FI） value was approximately 1.6. Three M. sativa cultivars, including Xunlu, Aergangjin and Junren, provided either Race 3 or 4 of H. glycines to develop into mature cysts with their FI value below 5 as well. Our results demonstrated that legume plants in both M. truncatula and M. sativa were not likely to be a model plant for H. glycines because of an extreme high resistance.

关键词： soybean cyst nematode alfalfa life cycle female index pathosystem

Correlations of β-catenin,Ki67 and Her-2/neu with gastric cancer

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Asian Pacific Journal of Tropical Medicine 2014年第4期7卷 257-261页

作者： Hong-wen Wu Cheng-Yong Qin Ji-Lai Huang Xian-Yi Kong wen-Ji Wang wen-kun Bai School of Medicine Shandong University Digestive Internal Medicine The First Hospital of Zibo City Digestive Internal Medicine Shandong Provincial Hospital Digestive Internal Medicine Shandong Qianfoshan Hospital

Objective:To study the clinical pathologic characteristics ofβ-catenin,Ki67 and Her-2/neu in gastric cancer and the correlation ofβ-catenin and Ki67 to the protein expression and gene conditions of Her-2/***:The protein expression ofβ-catenin,Ki67 and Her-2/Neu was detected by immunohistochemistry in 101 cases of gastric cancer and the gene conditions of Her-2/Neu by fluorescence in situ hybridization(FISH).Results:The protein expression ofβ-catenin,Ki67 and Her-2/Neu had close relationship with the clinical pathologic characteristics of gastric ***β-catenin and Ki67 had obvious correlation to the differentiation,infiltration and lymphatic metastasis of the gastric cancer(P<0.05).The Ki67 had close relationship with the tumor-node-metastasis staging staging of gastric cancer(P<0.05).Her-2/Neu had close relationship with the differentiation and tumor-node-metastasis staging of gastric cancer(P<0.05)but had no relationship with the infiltration and lymphatic metastasis of the gastric cancer(P<0.05).The protein expression of Ki67 had significantly positive correlation to the protein expression and gene amplification conditions of Her-2/Neu(r=0.567,P<0.05 for protein;r=0.304,P<0.05 for gene).Conclusions:Combined detection ofβ-catenin,Ki67 and Her-2/Neu can be used as a reliable method to help the observation of biological behavior,diagnosis and prognosis of gastric cancer,and Ki67 can be used to serve the preliminary screening of Her-2/Neu gene state.

关键词： Gastric cancer β-catenin Ki67 Her-2/neu Immunohistochemistry Fluorescence in situ hybridization Tumor marker