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Knock-in of exogenous sequences based on CRISPR/Cas9 targeting autosomal genes and sex chromosomes in the diamondback moth,Plutella xylostella

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Journal of Integrative Agriculture 2024年第9期23卷 3089-3103页

作者： Shanyu Li Guifang Lin Haoqi Wen Haiyan Lu Anyuan Yin Chanqin Zheng Feifei Li Qingxuan Qiao Lu Jiao Ling Lin Yi Yan Xiujuan Xiang Huang Liao Huiting Feng Yussuf Mohamed Salum Minsheng You Wei Chen weiyi he State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops/Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops/International Joint Research Laboratory of Ecological Pest Control Ministry of EducationInstitute of Applied EcologyFujian Agriculture and Forestry UniversityFuzhou 350002China Ganzhou Key Laboratory of Greenhouse Vegetable Gannan Normal UniversityGanzhou 341000China

Genetic pest control strategies based on precise sex separation and only releasing sterile males can be accomplished by site-specific genome *** the current study,we showed that the mutation of single-allele Pxfl(2)d can significantly impair the normal mating behavior and testis development in male adults of the notorious cruciferous insect pest Plutella xylostella,in addition to its known functions in the ovarian development in female adults and egg *** CRISPR/Cas9-based knock-in experiments revealed that site-specific integration of an exogenous green fluorescent protein(GFP)gene into autosomal Pxfl(2)d for labelling mutants could be ***,this gene is not a suitable target for GFP insertion to establish a genetically stable knock-in strain because of the severe decline in reproductive *** further screened for the W-chromosome-linked and Z-chromosome-linked regions to test the knock-in efficiency mediated by CRISPR/*** results verified that both types of chromosomes can be targeted for the site-specific insertion of exogenous *** ultimately obtained a homozygous knock-in strain with the integration of both Cas9 and cyan fluorescent protein(CFP)expression cassettes on a Z-linked region in ***,which can also be used for early sex *** injecting the sgRNA targeting Pxfl(2)d alone into the eggs laid by female adults of the Z-Cas9-CFP strain,the gene editing efficiency reached 29.73%,confirming the success of expressing a functional Cas9 *** together,we demonstrated the feasibility of the knock-in of an exogenous gene to different genomic regions in ***,while the establishment of a heritable strain required the positioning of appropriate *** study provides an important working basis and technical support for further developing genetic strategies for insect pest control.

关键词： cruciferous specialist fl(2)d CRISPR/Cas9 knock-in sex chromosome

基于环境DNA宏条形码技术的克拉里昂-克利珀顿区鱼类多样性

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应用海洋学学报 2024年第3期43卷 429-441页

作者：何韦宜黄浩王磊欧丹云张延续李伟文自然资源部第三海洋研究所海洋生态保护与修复重点实验室福建厦门361005

海洋鱼类长期遭受过度捕捞的影响,导致生物多样性丧失。在公海区域鱼类多样性研究常用传统的调查方法,该方法主要用于商业物种的调查。环境DNA(eDNA)已成功用于监测水生环境中的生物多样性,本研究通过eDNA宏条形码技术,监测采矿前东太... 详细信息

海洋鱼类长期遭受过度捕捞的影响,导致生物多样性丧失。在公海区域鱼类多样性研究常用传统的调查方法,该方法主要用于商业物种的调查。环境DNA(eDNA)已成功用于监测水生环境中的生物多样性,本研究通过eDNA宏条形码技术,监测采矿前东太平洋克拉里昂-克利珀顿区(CC区)的鱼类多样性信息。通过使用两种鱼类通用引物(MiFish、COⅠ)获取CC区的鱼类多样性,共检测出140属、132种鱼类,随水深增加,物种检出率逐渐降低。其中,基于MiFish引物的eDNA宏条形码技术鉴定出2纲、35目、62科、88属、57种鱼类。基于COⅠ引物的共鉴定出2纲、29目、57科、72属、87种鱼类。基于COⅠ引物的检测出濒临灭绝物种有日本鳗鲡(Anguilla japonica)、美洲鳗鲡(Anguilla rostrata)、豹纹窄尾魟(Himantura uarnak)、褐背蝠鲼(Mobula tarapacana);极度濒危物种有欧洲鳗鲡(Anguilla anguilla);易危物种有大西洋蓝枪鱼(Makaira nigricans)、扁鲹(Pomatomus saltatrix);近危物种有大青鲨(Prionace glauca)。基于MiFish引物的检测出近危物种有大青鲨、龙头鱼(Harpadon nehereus)、尖头斜齿鲨(Scoliodon laticaudus),易危物种有褐点石斑(Epinephelus fuscoguttatus)。MiFish引物检测到的鱼类科、属数量高于COⅠ引物的检测结果,针对OTU序列的属及以上的分类具有高效的覆盖率和分辨率。研究结果表明,eDNA宏条形码技术可以有效鉴定鱼类物种,在海洋鱼类多样性监测中具有良好的应用前景。eDNA宏条形码技术作为一种非侵入性方法,监测到的鱼类多样性远高于深海生物诱捕观测器捕获的鱼类多样性,同时基于MiFish引物的eDNA宏条形码技术监测鱼类物种更具优势。

关键词：海洋生物学鱼类多样性环境DNA 克拉里昂-克利珀顿区东太平洋

风湿性心脏瓣膜病患者术后自我感受负担与心理脆弱程度的相关性及预后的影响因素

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中国临床研究 2024年第10期37卷 1593-1597页

作者：白娅娜徐宝玲唐嘉佑何微谊空军军医大学第一附属医院心血管外科陕西西安710032

目的调查风湿性心脏瓣膜病患者术后自我感受负担及心理脆弱程度,分析二者之间的关系及患者预后的影响因素。方法选取2019年6月至2022年6月于西京医院手术治疗的88例风湿性心脏瓣膜病患者作为研究对象。采用一般资料调查表以及自我感受... 详细信息

目的调查风湿性心脏瓣膜病患者术后自我感受负担及心理脆弱程度,分析二者之间的关系及患者预后的影响因素。方法选取2019年6月至2022年6月于西京医院手术治疗的88例风湿性心脏瓣膜病患者作为研究对象。采用一般资料调查表以及自我感受负担量表(SPBS)、心理脆弱量表(MVQ)调查患者一般情况、自我感受负担与心理脆弱程度。采用门诊复诊或电话进行随访,了解患者术后全因死亡、因不良心血管事件住院或死亡情况,随访时间截至2023年6月。根据术后全因死亡、因不良心血管事件住院或死亡与否分为预后良好组(n=65)和预后不良组(n=23)。Pearson相关性分析风湿性心脏瓣膜病患者术后自我感受负担与心理脆弱程度的关系,对比两组相关临床资料、SPBS评分和MVQ评分,logistic多因素回归分析风湿性心脏瓣膜病患者术后预后不良的影响因素。结果两组年龄、术前心功能分级、SPBS评分、MVQ评分对比,差异均有统计学意义(P<0.05)。Logistic多因素回归分析显示,年龄≥60岁(OR=1.487,P=0.002)、术前心功能分级为Ⅲ~Ⅳ级(OR=1.709,P=0.039)、SPBS评分(OR=1.180,P=0.016)和MVQ评分升高(OR=1.110,P=0.043)均是风湿性心脏瓣膜病患者术后预后不良的独立影响因素。Pearson相关性分析显示,风湿性心脏瓣膜病患者SPBS评分与MVQ评分呈正相关(r=0.589,P<0.01)。结论风湿性心脏瓣膜病患者术后SPBS评分与MVQ评分具有相关性,年龄大、术前心功能分级高、SPBS评分及MVQ评分高均是其预后不良的独立危险因素。

关键词：风湿性心脏瓣膜病心脏瓣膜置换术自我感受负担心理脆弱程度预后

A Fast Insertion Tabu Search with Conflict-Avoidance heuristic for the Multisatellite Multimode Crosslink Scheduling Problem

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Tsinghua Science and Technology 2024年第3期29卷 843-862页

作者： weiyi Yang Lei he Xiaolu Liu Weican Meng Yingwu Chen College of Systems Engineering National University of Defense TechnologyChangsha 410000China Beijing Institude of Tranking and Telecommunication Technology Beijing 100000China

An agile earth-observing satellite equipped with multimode cameras capable of transmitting observation data to other satellites is developed to rapidly respond to requests with multiple observation *** gives rise to the Multisatellite Multimode Crosslink Scheduling(MMCS)problem,which involves allocating observation requests to agile satellites,selecting appropriate timing and observation modes for the requests,and transmitting the data to the ground station via the satellite communication ***,a mixed integer programming model is introduced to include all complex time and operation *** solve the MMCS problem,a two-stage heuristic method,called Fast insertion Tabu Search with Conflict-avoidance(FTS-C)heuristic,is *** the first stage,a conflict-avoidance insertion algorithm is designed to generate a high-quality initial solution by considering the requests transmission and ***,the tabu search-based second stage optimizes the initial ***,an extensive empirical study based on a real-world situation demonstrates that FTS-C can generate a solution with higher quality in less time than other state-of-the-art algorithms and the CPLEX solver.

关键词： earth observation satellites scheduling tabu search heuristic data transmission and download mixedinteger programming model

Genomic Variations in the Tea Leafhopper Reveal the Basis of Its Adaptive Evolution

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Genomics, Proteomics & Bioinformatics 2022年第6期20卷 1092-1105页

作者： Qian Zhao Longqing Shi weiyi he Jinyu Li Shijun You Shuai Chen Jing Lin Yibin Wang Liwen Zhang Guang Yang Liette Vasseur Minsheng You State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops Institute of Applied EcologyFujian Agriculture and Forestry UniversityFuzhou 350002China Institute of Rice Fujian Academy of Agricultural SciencesFuzhou 350018China Tea Research Institute Fujian Academy of Agricultural SciencesFuzhou 350001China Center for Genomics and Biotechnology Fujian Agriculture and Forestry UniversityFuzhou 350002China Department of Biological Sciences Brock UniversitySt.CatharinesON L2S 3A1Canada

Tea green leafhopper(TGL),Empoasca onukii,is of biological and economic *** numerous studies,the mechanisms underlying its adaptation and evolution remain ***,we use previously untapped genome and population genetics approaches to examine how the pest adapted to different environmental variables and thus has expanded *** complete a chromosome-level assembly and annotation of the *** genome,showing notable expansions of gene families associated with adaptation to chemoreception and *** signals indicating balancing selection highlight metabolic pathways involved in adaptation to a wide range of tea varieties grown across ecologically diverse *** of genetic variations among 54 *** samples unveil the population structure and evolutionary history across different tea-growing regions in *** results demonstrate that the genomic changes in key pathways,including those linked to metabolism,circadian rhythms,and immune system functions,may underlie the successful spread and adaptation of *** work highlights the genetic and molecular basis underlying the evolutionary success of a species with broad economic impacts,and provides insights into insect adaptation to host plants,which will ultimately facilitate more sustainable pest management.

关键词： Tea green leafhopper Genomic variation Population genetics Local adaptation Evolutionary history

Data Driven Vibration Control:A Review

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IEEE/CAA Journal of Automatica Sinica 2024年第9期11卷 1898-1917页

作者： weiyi Yang Shuai Li Xin Luo Chongqing Institute of Green and Intelligent Technology Chinese Academy of SciencesChongqing 400714 Chongqing School University of Chinese Academy of SciencesChongqing 400714China Faculty of Information Technology and Electrical Engineering University of OuluOulu 90570 VTT-Technology Research Center of Finland Oulu 90570Finlan College of Computer and Information Science Southwest UniversityChongqing 400715China

With the ongoing advancements in sensor networks and data acquisition technologies across various systems like manufacturing,aviation,and healthcare,the data driven vibration control(DDVC)has attracted broad interests from both the industrial and academic *** shaping(IS),as a simple and effective feedforward method,is greatly demanded in DDVC *** convolves the desired input command with impulse sequence without requiring parametric dynamics and the closed-loop system structure,thereby suppressing the residual vibration *** on a thorough investigation into the state-of-the-art DDVC methods,this survey has made the following efforts:1)Introducing the IS theory and typical input shapers;2)Categorizing recent progress of DDVC methods;3)Summarizing commonly adopted metrics for DDVC;and 4)Discussing the engineering applications and future trends of *** doing so,this study provides a systematic and comprehensive overview of existing DDVC methods from designing to optimizing perspectives,aiming at promoting future research regarding this emerging and vital issue.

关键词： Data driven vibration control(DDVC) data science designing method feedforward control industrial robot input shaping optimizing method residual vibration

Comparison of Adaptive Simulation Observation Experiments of the heavy Rainfall in South China and Sichuan Basin

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Advances in Atmospheric Sciences 2024年第11期41卷 2173-2191页

作者： Linbin he weiyi PENG Yu ZHANG Shiguang MIAO Siqi CheN Jiajing LI Duanzhou SHAO Xutao ZHANG College of Ocean and Meteorology Guangdong Ocean UniversityZhanjiang 524088China Chinese Academy of Meteorology Sciences Beijing 100081China Institute of Urban Meteorology China Meteorological AdministrationBeijing 100089China School of Marine Sciences Nanjing University of Information Science and TechnologyNanjing 210044China Dalian Marine Center Ministry of Nature Resources of the People's Republic of ChinaDalian 116001China

This study examines the effectiveness of adaptive observation experiments using the ensemble transformation sensitivity(ETS) method to improve precipitation forecasts during heavy rainfall events in South China and the Sichuan Basin. High-resolution numerical models are employed to simulate adaptive observations. By identifying the sensitive areas of key weather system positions 42 hours before heavy rainfall events, the adaptive observations improve the prediction of jet streams, strong winds, and shear lines, which are essential for accurate heavy rainfall forecasting. This improvement is reflected in both the precipitation structure and location accuracy within the verification region. In South China, targeted observations enhance rainfall predictions by improving water vapor transport. In the Sichuan Basin, adaptive observations refine water vapor transport and adjust vortex dynamics. This research highlights the importance of accurately predicting shear lines and jet streams for forecasting heavy rainfall in these areas. Overall, this study found that adaptive observation enhances the precipitation forecast skills of the structure and location for heavy rainfall in South China and the Sichuan Basin, emphasizing their potential utility in operational numerical weather prediction.

关键词： adaptive observation ensemble transform sensitivity data assimilation rainfall

微塑料污染对东太平洋鲣肌肉组织代谢的潜在影响

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渔业研究 2024年第1期46卷 49-59页

作者：吴丽军潘钟李伟文戴小杰何韦宜黄浩欧丹云王磊上海海洋大学海洋科学学院上海201306 自然资源部第三海洋研究所福建厦门361005

微塑料污染遍布全球,其可随摄食或呼吸等方式进入生物体。本研究设置实验组和对照组,采用超高效液相色谱-质谱联用技术对东太平洋鲣(Katsuwonus pelamis)肌肉组织进行了非靶向代谢组学分析,以研究微塑料污染对其代谢的潜在影响。结果显... 详细信息

微塑料污染遍布全球,其可随摄食或呼吸等方式进入生物体。本研究设置实验组和对照组,采用超高效液相色谱-质谱联用技术对东太平洋鲣(Katsuwonus pelamis)肌肉组织进行了非靶向代谢组学分析,以研究微塑料污染对其代谢的潜在影响。结果显示,共有34个代谢产物发生显著性差异,其中19个差异代谢物上调、15个差异代谢物下调。差异代谢物分析表明,甲基丙二酸和丙酸上调可诱发神经疾病,可能是造成鲣游泳模式异常的原因;苹果酸下调可能使鲣面临细菌的感染风险;相反,甘油-3-磷酸、磷酸肌酸和羟基积雪草苷上调则是鲣应对微塑料积累的一种自身保护机制。代谢通路富集分析表明,差异代谢物主要富集于新陈代谢、胆汁分泌、丙酸酯代谢、近端小管碳酸氢盐回收、乙苯降解、甘油磷脂代谢和胆固醇代谢。研究结果可为微塑料污染对海洋鱼类的毒理学研究提供一定的参考。

关键词：鲣微塑料代谢产物东太平洋

Three-dimensional(3D)parametric measurements of individual gravels in the Gobi region using point cloud technique

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Journal of Arid Land 2024年第4期16卷 500-517页

作者： JING Xiangyu HUANG weiyi KAN Jiangming College of Engineering Beijing Forestry UniversityBeijing 100083China Key Laboratory of State Forestry Administration on Forestry Equipment and Automation Beijing 100083China

Gobi spans a large area of China,surpassing the combined expanse of mobile dunes and semi-fixed *** presence significantly influences the movement of sand and ***,the complex origins and diverse materials constituting the Gobi result in notable differences in saltation processes across various Gobi *** is challenging to describe these processes according to a uniform ***,it becomes imperative to articulate surface characteristics through parameters such as the three-dimensional(3D)size and shape of *** morphology information for Gobi gravels is essential for studying its genesis and sand *** enhance the efficiency and information yield of gravel parameter measurements,this study conducted field experiments in the Gobi region across Dunhuang City,Guazhou County,and Yumen City(administrated by Jiuquan City),Gansu Province,China in March 2023.A research framework and methodology for measuring 3D parameters of gravel using point cloud were developed,alongside improved calculation formulas for 3D parameters including gravel grain size,volume,flatness,roundness,sphericity,and equivalent grain *** multi-view geometry technology for 3D reconstruction allowed for establishing an optimal data acquisition scheme characterized by high point cloud reconstruction efficiency and clear ***,the proposed methodology incorporated point cloud clustering,segmentation,and filtering techniques to isolate individual gravel point *** point cloud algorithms,including the Oriented Bounding Box(OBB),point cloud slicing method,and point cloud triangulation,were then deployed to calculate the 3D parameters of individual *** systematic processes allow precise and detailed characterization of individual *** gravel grain size and volume,the correlation coefficients between point cloud and manual measurements all exceeded 0.9000,confirming the feasibility of the proposed methodol

关键词： Gobi gravels three-dimensional(3D)parameters point cloud 3D reconstruction Random Sample Consensus(RANSAC)algorithm Density-Based Spatial Clustering of Applications with Noise(DBSCAN)