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The R2R3-MYB transcription factor GaPC controls petal coloration in cotton

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The Crop Journal 2023年第5期11卷 1319-1330页

作者： Caiping Cai Fan Zhou weixi li Yujia Yu Zhihan Guan Baohong Zhang Wangzhen Guo State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization Nanjing Agricultural UniversityNanjing 210095JiangsuChina Engineering Research Center of Ministry of Education for Cotton Germplasm Enhancement and Application Nanjing Agricultural UniversityNanjing 210095JiangsuChina Department of Biology East Carolina UniversityGreenvilleNC 27858USA

Although a few cases of genetic epistasis in plants have been reported, the combined analysis of genetically phenotypic segregation and the related molecular mechanism remains rarely studied. Here, we have identified a gene(named GaPC) controlling petal coloration in Gossypium arboreum and following a heritable recessive epistatic genetic model. Petal coloration is controlled by a single dominant gene,GaPC. A loss-of-function mutation of GaPC leads to a recessive gene Gapc that masks the phenotype of other color genes and shows recessive epistatic interactions. Map-based cloning showed that GaPC encodes an R2R3-MYB transcription factor. A 4814-bp long terminal repeat retrotransposon insertion at the second exon led to GaPC loss of function and disabled petal coloration. GaPC controlled petal coloration by regulating the anthocyanin and flavone biosynthesis pathways. Expression of core genes in the phenylpropanoid and anthocyanin pathways was higher in colored than in white petals. Petal color was conferred by flavonoids and anthocyanins, with red and yellow petals rich in anthocyanin and flavonol glycosides, respectively. This study provides new insight on molecular mechanism of recessive epistasis,also has potential breeding value by engineering GaPC to develop colored petals or fibers for multifunctional utilization of cotton.

关键词： Cotton Petal color R2R3-MYB transcription factor LTR-RT insertion Flavonoid/anthocyanin biosynthesis Recessive epistasis

Suppressing a mitochondrial calcium uniporter activates the calcium signaling pathway and promotes cell elongation in cotton

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The Crop Journal 2024年第2期12卷 411-421页

作者： Yujia Duan Xiaoguang Shang Ruiping Tian weixi li Xiaohui Song Dayong Zhang Wangzhen Guo State Key Laboratory of Crop Genetics&Germplasm Enhancement and Utilization Nanjing Agricultural UniversityNanjing 210095JiangsuChina Engineering Research Center of Ministry of Education for Cotton Germplasm Enhancement and Application Nanjing Agricultural UniversityNanjing 210095JiangsuChina

Mitochondrial calcium uniporter(MCU)is a conserved calcium ion(Ca^(2+))transporter in the mitochondrial inner membrane of eukaryotic *** MCU proteins regulate Ca^(2+)flow and modulate plant cell development remain largely ***,we identified the gene GhMCU4 encoding a MCU protein that negatively regulates plant development and fiber elongation in cotton(Gossypium hirsutum).GhMCU4expressed constitutively in various tissues with the higher transcripts in elongating fiber *** of GhMCU4 in cotton significantly elevated the plant height and root *** calcium signaling pathway was significantly activated and calcium sensor genes,including Ca^(2+)dependent modulator of interactor of constitutively active ROP(GhCMI1),calmodulin like protein(GhCML46),calciumdependent protein kinases(GhCPKs),calcineurin B-like protein(GhCBLs),and CBL-interacting protein kinases(GhCIPKs),were dramatically upregulated in GhMCU4-silenced *** processes were preferentially enriched,and genes related to regulation of transcription were upregulated in GhMCU4-silenced *** contents of Ca^(2+)and H_(2)O_(2)were significantly increased in roots and leaves of GhMCU4-silenced *** length and Ca^(2+)and H_(2)O_(2)contents in fibers were significantly increased in GhMCU4-silenced *** study indicated that GhMCU4 plays a negative role in regulating cell elongation in cotton,thus expanding understanding in the role of MCU proteins in plant growth and development.

关键词： Calcium signaling Hydrogen peroxide Metabolic processed Gossypium hirsutum

Enhancement and recovery of photoluminescence and stability by multifunctional etching ligands treatment for perovskite nanocrystals

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Journal of Energy Chemistry 2023年第1期76卷 495-502,I0013页

作者： Yang Zhao Haipeng Zeng Xueqing Cai Yingshan Zhou Yangming Jiang Xin Zheng lin li Shujing Zhang Long Luo weixi li Xiaoru Zhang Ranran liu Wenxi liang Rui Guo Xiong li Michael Gratzel Center for Mesoscopic Solar Cells Wuhan National Laboratory for OptoelectronicsHuazhong University of Science and TechnologyWuhan 430074HubeiChina State Key Laboratory of New Textile Materials and Advanced Processing Technologies Wuhan Textile UniversityWuhan 430073HubeiChina State Key Laboratory of Functions and Applications of Medicinal Plants Guizhou Medical University Guiyang 550014GuizhouChina

Perovskite nanocrystals(PNCs)have recently become promising optoelectronic materials due to their excellent photophysical ***,the highly dynamic binding state between ligands and the surface of PNCs has severely restricted their luminescent properties and *** this work,1,3-bisbenzyl-2-oxoimidazolidine-4,5-dicarboxylic acid(cycle acid,CA)is introduced as both an etchant and a ligand upon post-synthetic surface treatment of *** removing the imperfect octahedrons[Pb X_(6)]^(4-)and passivating the surface defects synergistically,this treatment improves photoluminescence quantum yields from 76%to 95%and enhances the stability of PNCs against polar solvent,moisture,heat,and ***,CA can effectively and instantly recover the luminescence emission for aged *** a result,the CA-Cs Pb Br_(3)PNCs and CA-Cs Pb IxBr_(3-x)PNCs are applied as color-converting layers on a blue LED chip for warm white light-emitting diodes(WLEDs)with a color coordinate of(0.41,0.40).Importantly,the CA-based WLED device exhibits superior stability in operational conditions.

关键词： Perovskite nanocrystals Synergistic effect Emission recovery White light emitting diodes Stability

Fokker-Planck算子和Witten Laplacian的谱分析

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中国科学：数学 2019年第2期49卷 161-174页

作者：李维喜武汉大学数学与统计学院武汉430072

本文主要介绍如何应用经典以及最新发展的亚椭圆工具来分析来源于数学物理问题的一类算子的谱性质.特别地,本文将重点分析Fokker-Planck算子和Witten Laplacian这两个算子预解式紧性的判别法则以及两者之间的关系.

关键词：谱理论亚椭圆技巧 Fokker-Planck Witten Laplacian 预解式紧性判别法则

Screening and Verification of Genes Specifically Responding to Continuous Cropping Obstacle in Rehmannia glutinosa L.

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Agricultural Biotechnology 2019年第5期8卷 12-18页

作者： Xiaoran WANG Zheng li Fangming liU weixi li Yuhong WANG Xinjian CHEN College of life Sciences Henan Agricultural UniversityZhengzhou 450002China College of Forestry Henan Agricultural UniversityZhengzhou 450002China

Rehmannia glutinosa *** one of the important medicinal crops in *** cropping obstacle severely restricts the yield and quality of ***,but its molecular mechanism is still *** this study,with widely-planted "Wen 85-5" as an experiment material,based on the digital gene expression profiling (DGE) data of previous five stress treatments (continuous cropping,phenolic acid,salt,drought and waterlogging) and the first cropping and continuous cropping treatments of *** in five different periods (seedling period,elongation period,early expanding period,middle expanding period and later expanding period),80 candidate genes (|log 2 ratio|≥1,FDR lic acid and p-hydroxybenzonic acid were used to treat aseptic plantlets of ***,respectively,and it was confirmed through qRT-PCR that the expression levels of some genes under phenolic acid treatment changed more severely than that under the continuous cropping soil extract treatment,and four key genes involved in the response of *** to continuous cropping were finally *** study lays a foundation for further exploration of the molecular mechanism of continuous cropping obstacle.

关键词： Rehmannia glutinosa L. Continuous cropping obstacle Response gene Soil extract Phenolic acid treatment

Advances of Allelopathic Autotoxicity in Rehmannia glutinosa L.

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Agricultural Biotechnology 2019年第3期8卷 46-50,53页

作者： Xiaoran WANG weixi li Zhen li Yuhong WANG Zhongyi ZHANG Xinjian CHEN College of life Sciences Henan Agricultural UniversityZhengzhou 450002China College of Crop Sciences Fujian Agriculture and Forestry UniversityFuzhou 350002China Key Laboratory for Genetics Breeding and Multiple Utilization of Crops Ministry of Education Fujian Agriculture and Forest UniversityFuzhou 350002China

Allelopathic autotoxicity occurs when a plant releases toxic chemical substances into the environment which inhibits development and growth of the same plant *** glutinosa L.( *** ) is one of the most common traditional Chinese medicines,whose productivity and quality,however,are seriously impacted by consecutive monoculture *** autotoxicity is one reason for consecutive monoculture *** this paper,we reviewed the categories of allelochemicals,the methods of allelochemicals identification,and the mechanisms of allelopathic autotoxicity,which provides clues for further study of the molecular mechanisms of allelopathic autotoxicity and consecutive monoculture obstacle.

关键词： Rehmannia glutinosa Allelopathic autotoxicity Consecutive monoculture obstacle Autotoxins Self-DNA

19.59%Efficiency from Rb_(0.04)-Cs_(0.14)FA_(0.86)Pb(Br_(y)I_(1−y))_(3) perovskite solar cells made by vapor-solid reaction technique

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Science Bulletin 2021年第10期66卷 962-964,M0003页

作者： Long Luo Zhiliang Ku weixi li Xin Zheng Xiong li Fuzhi Huang Yong Peng liming Ding Yi-Bing Cheng State Key Laboratory of Advanced Technologies for Materials Synthesis and Processing Wuhan University of TechnologyWuhan 430070China Wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhan 430074China Foshan Xianhu Laboratory of the Advanced Energy Science and Technology Guangdong Laboratory Foshan 528216China Center for Excellence in Nanoscience(CAS) Key Laboratory of Nanosystem and Hierarchical Fabrication(CAS)National Center for Nanoscience and TechnologyBeijing 100190China

Organic-inorganic hybrid perovskite materials have recently attracted extensive attentions because of their intriguing optoelectronic properties for photovoltaics[1],[2],[3].The power conversion efficiency(PCE)of pero... 详细信息

关键词：太阳电池离子迁移钙钛矿薄膜气相反应迁移速率组件效率反应工艺

气相色谱电子捕获检测器检测蟑螂头部痕量章鱼胺的衍生物

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分析化学 2006年第1期34卷 62-64页

作者：潘灿平李维喜汪泳三江树人中国农业大学理学院中国农业大学理学院北京100094

章鱼胺（OA）是昆虫体内的痕量神经活性物质。本实验研究了蟑螂头部基质中OA的提取、章鱼胺与五氟丙酸酐的衍生化反应,优化了最佳衍生反应条件。建立了适合于昆虫样本中OA痕量测定的衍生化气相色谱-电子捕获检测方法。昆虫头部匀浆样本... 详细信息

章鱼胺（OA）是昆虫体内的痕量神经活性物质。本实验研究了蟑螂头部基质中OA的提取、章鱼胺与五氟丙酸酐的衍生化反应,优化了最佳衍生反应条件。建立了适合于昆虫样本中OA痕量测定的衍生化气相色谱-电子捕获检测方法。昆虫头部匀浆样本在10 ng/g OA添加浓度时回收率在89%;18%;该方法的最低检出浓度为1.7ng/g。采用GC-MS对衍生化产物进行了定性确证分析。

关键词：章鱼胺痕量分析衍生化气相色谱电子捕获检测

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提高矿井主排水系统管网运行效率的探讨

给水排水 2009年第3期35卷 110-112页

作者：张猛李维熙中国矿业大学徐州221008 焦作煤业(集团)有限责任公司焦作454159

矿井排水系统是矿井安全生产的重要保证。在保证安全和高效运行的同时,应最大限度提高综合效率,达到降低排水电耗的目的。以经济流速为基础,使管网的造价和运行费用之和最小。通过大量的试验和研究,对不同的管径和排水模式进行了详细对... 详细信息

矿井排水系统是矿井安全生产的重要保证。在保证安全和高效运行的同时,应最大限度提高综合效率,达到降低排水电耗的目的。以经济流速为基础,使管网的造价和运行费用之和最小。通过大量的试验和研究,对不同的管径和排水模式进行了详细对比和分析,得出多管并联排水时,水泵效率、管网效率、系统效率均明显提高的结论。

关键词：矿井排水系统管网特性经济流速多管并联排水效率