Suppressing a mitochondrial calcium uniporter activates the calcium signaling pathway and promotes cell elongation in cotton

作     者：Yujia Duan Xiaoguang Shang Ruiping Tian Weixi Li Xiaohui Song Dayong Zhang Wangzhen Guo Yujia Duan;Xiaoguang Shang;Ruiping Tian;Weixi Li;Xiaohui Song;Dayong Zhang;Wangzhen Guo

作者机构：State Key Laboratory of Crop Genetics&Germplasm Enhancement and UtilizationNanjing Agricultural UniversityNanjing 210095JiangsuChina Engineering Research Center of Ministry of Education for Cotton Germplasm Enhancement and ApplicationNanjing Agricultural UniversityNanjing 210095JiangsuChina 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2024年第12卷第2期

页      面：411-421页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by National Key Research and Development Program of China(2022YFD1200300) Jiangsu Key R&D Program(BE2022384) the Collaborative Innovation Center for Modern Crop Production co-sponsored by Province and Ministry(CIC-MCP)(No.10) 

主　　题：Calcium signaling Hydrogen peroxide Metabolic processed Gossypium hirsutum 

摘      要：Mitochondrial calcium uniporter(MCU)is a conserved calcium ion(Ca^(2+))transporter in the mitochondrial inner membrane of eukaryotic *** MCU proteins regulate Ca^(2+)flow and modulate plant cell development remain largely ***,we identified the gene GhMCU4 encoding a MCU protein that negatively regulates plant development and fiber elongation in cotton(Gossypium hirsutum).GhMCU4expressed constitutively in various tissues with the higher transcripts in elongating fiber *** of GhMCU4 in cotton significantly elevated the plant height and root *** calcium signaling pathway was significantly activated and calcium sensor genes,including Ca^(2+)dependent modulator of interactor of constitutively active ROP(GhCMI1),calmodulin like protein(GhCML46),calciumdependent protein kinases(GhCPKs),calcineurin B-like protein(GhCBLs),and CBL-interacting protein kinases(GhCIPKs),were dramatically upregulated in GhMCU4-silenced *** processes were preferentially enriched,and genes related to regulation of transcription were upregulated in GhMCU4-silenced *** contents of Ca^(2+)and H_(2)O_(2)were significantly increased in roots and leaves of GhMCU4-silenced *** length and Ca^(2+)and H_(2)O_(2)contents in fibers were significantly increased in GhMCU4-silenced *** study indicated that GhMCU4 plays a negative role in regulating cell elongation in cotton,thus expanding understanding in the role of MCU proteins in plant growth and development.