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Simultaneous determination of 14 nitroimidazoles using thin-layer chromatography combined with surface-enhanced Raman spectroscopy(TLC-SERS)

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Food Bioscience 2022年第4期48卷 310-319页

作者： Siqian Shi Hang Yu Fangwei Yang weirong yao Yunfei Xie State Key Laboratory of Food Science and Technology Jiangnan UniversityChina School of Food Science and Technology Jiangnan UniversityChina Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina

Nitroimidazoles are a family of veterinary drugs containing a 5-nitroimidazole ring structure,which are commonly used *** view of their potential genotoxicity and possible coexistence in actual samples,it is necessary to establish a rapid and effective method for the simultaneous determination of multiple *** this study,a rapid thin-layer chromatography combined with surface-enhanced Raman spectroscopy(TLC-SER)detection method for 14 nitroimidazole compounds has been *** theoretical Raman spectra of the nitroimidazoles were calculated using the B3LYP/6-311+G(d)basis set in density functional theory(DFT)and the theoretical Raman spectra of TNZ,NBZ,ONZ,SCZ,DMZ,MNI,2-HMZ,4-HMZ,CMNI,IPZ,MNZ-OH and HMDMZ have been obtained for the first *** detection was carried out on a gold nanoparticles(AuNPs)substrate,which exhibited strong SERS signals and a limit of detection limit of 0.1 mg/*** optimizing the conditions used for the developing agent in TLC,12 nitroimidazoles were successfully separated,while MNZ and RNZ still could not be *** combined with the chemometrics analysis method,the TLC-SERS method can realize the simultaneous detection of 14 *** pork samples were spiked with nitroimidazoles with a recovery rate of 80%-110%and RSD of 0.74%-16.70%,which showed that SERS and TLC-SERS methods are also effective for detection in actual *** detection time of this method is<10 min,which is suitable for the rapid detection of nitroimidazoles,especially for the rapid qualitative identification of nitroimidazoles in situ.

关键词： Nitroimidazoles SERS TLC-SERS PCA

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Establishment of the thin-layer chromatography-surface-enhanced Raman spectroscopy and chemometrics method for simultaneous identification of eleven illegal drugs in anti-rheumatic health food

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Food Bioscience 2022年第5期49卷 556-568页

作者： Fangwei Yang Cheng Wang Hang Yu Yahui Guo Yuliang Cheng weirong yao Yunfei Xie State Key Laboratory of Food Science and Technology Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina School of Food Science and Technology Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina Joint International Research Laboratory of Food Safety Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina

The risk of the illegal addition of anti-inflammatory and analgesic chemical drugs in anti-rheumatic health foods should not be *** supervision and rapid on-site detection technology need to be ***-layer chromatography-surface-enhanced Raman spectroscopy(TLC-SERS),which has the advantages of simple operation,fast separation,and qualitative and quantitative detection,was used in this *** these eleven chemical drugs(acetaminophen,acetylsalicylic acid,aminophenazone,dexamethasone,diclofenac sodium,hydrocortisone,indometacin,naproxen,phenylbutazone,piroxicam,prednisone 21-acetate)that may be added to anti-rheumatic health foods have been simultaneously identified by TLC-SERS combined with chemometrics *** characteristic signals of the separated drug spots were collected by SERS,which were optimized by the gold colloidal nanoparticles’volume and integration *** SERS was subjected to principal component analysis(PCA)to reduce dimensionality and combined with the pattern recognition methods in chemometrics,such as PCA-Linear Discriminant Analysis(LDA),PCA-K Nearest Neighbor and PCA-Support Vector Machine,and eleven drug components were judged and ***,the predictive performances of different models were also analyzed and *** results showed that the TLC plate and four organic solvents of petroleum ether,chloroform,ethyl acetate and acetic acid were selected as the developing *** dropping amount of gold colloidal nanoparticles and the integration time were set and *** limit of detection of the simultaneous detection method of SERS was 10-100 mg/***,SERS was preprocessed by Gap-Segment 2nd Derivative,and then the PCA-LDA model was established,and the model’s prediction accuracy can reach 100%.The method is simple,rapid,sensitive and accurate,and has low experimental instruments and equipment *** is suitable for the on-site simultaneous detection of various anti-inflammato

关键词： TLC-SERS Health food Illegal drugs Chemometrics Simultaneous identification Rapid detection

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Trans-cinnamaldehyde inhibits Penicillium italicum by damaging mitochondria and inducing apoptosis mechanisms

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Food Science and Human Wellness 2022年第4期11卷 975-981页

作者： Fangwei Yang Jiaqi Mi Fei Huang Prompong Pienpinijtham Yahui Guo Yuliang Cheng weirong yao Yunfei Xie School of Food Science and Technology Jiangnan UniversityWuxi 214122China State Key Laboratory of Food Science and Technology Jiangnan UniversityWuxi 214122China Qingdao Special Food Research Institute Qingdao 266109ShandongChina

Plant-derived essential oils have excellent antifungal effects and can be used for the preservation of fresh foods such as fruits and vegetables, but the detailed mechanism has not been fully elucidated. In this study, we investigated the inhibitory effects of trans-cinnamaldehyde on Penicillium italicum, a common pollution fungus in citrus, and explored the antifungal mechanism of trans-cinnamaldehyde by detecting fungal oxidative damage, mitochondrial metabolism, and cell apoptosis. These results showed that transcinnamaldehyde made the carboxylic acid cycle deregulated by altering the related enzyme activities(succinate dehydrogenase, malate dehydrogenase) and mid product. Moreover, the level of reactive oxygen species rose sharply while the redox level was out of regulation. The mitochondrial membrane potential collapsed, leading to the leakage of cytochrome c, and then triggering the activation of apoptotic protease, which was further confirmed by the significant increase in caspase-3 activity from(3.6 ± 0.6) U to(8.8 ± 1.1) U(P < 0.05). The cytochrome c in mitochondria was detected by confocal Raman microspectroscopy, the characteristic intensity index(I750/I2944) was decreased, indicating that the cytochrome c in mitochondria was reduced and leakage. Besides, the strong negative correlation between Raman intensity and the amount of cytochrome c leakage was established with the correlation coefficient of-0.981 7. This study revealed that destroying the integrity of the mitochondrial membrane, activating the mitochondrial-mediated apoptosis pathway was the in-depth antifungal mechanism of trans-cinnamaldehyde;and Raman spectroscopy technology provided new ideas to study this process with high sensitivity determination of cytochrome c.

关键词： trans-cinnamaldehyde Penicillium italicum Raman microspectroscopy Inhibition mechanism Cytochrome c Apoptosis

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Study of the anti-fatigue properties of macamide,a key component in maca water extract,through foodomics and gut microbial genomics

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Food Bioscience 2022年第5期49卷 1265-1275页

作者： Chang Liu Hanyi Hua Hongkang Zhu Wenqian Xu Yahui Guo weirong yao He Qian Yuliang Cheng State Key Laboratory of Food Science and Technology Jiangnan UniversityNo.1800 Lihu AvenueWuxiJiangsu Province214122China School of Food Science and Technology Jiangnan UniversityNo.1800 Lihu AvenueWuxiJiangsu Province214122China Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province Jiangnan UniversityNo.1800 Lihu AvenueWuxiJiangsu Province214122China

Lepidium meyenii *** a type of food used to fight fatigue,and macamides is the key anti-fatigue component in Lepidium meyenii *** extract(Maca).The mechanisms of action of several foods with anti-fatigue properties lie in the oxidative balance of tissues;however,little attention has been given to the *** gut microbial community is closely related to dietary choices,as well as exercise-induced skeletal muscle *** such,this study used a foodomics approach to analyze the anti-fatigue properties of maca amide,a key component in maca,on the gut *** key components in maca were identified,and after validation in a mouse model,an analysis of the gut microbiome was *** results of database searches and network analyses showed that the key anti-fatigue component of maca was N-benzyl-9Z,12Z-octadecadienamide(Mm).Further analyses revealed that the key microbes metabolizing Mm belonged to Alistipes,Alloprevotella,Parabacteroides,and Butyricimonas genera and the Prevotellaceae in *** results of microbial analysis showed that Mm could improve exercise-induced skeletal muscle fatigue in mice by modulating the L-glutamate-ornithine-proline axis through the breakdown of histidine,arginine,and ***,from foodomics and gut microbial genomics perspectives,this study demonstrates that macamide is the key anti-fatigue component in maca,and these findings provide new insights for the development of foods that fight fatigue.

关键词： Maca N-Benzyl-9Z 12Z-octadecadienamide Foodmics Gut microbial genomics Anti-fatigue

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Protein corona formed on the TiO_(2)nanoparticles promotes the hydrolysis of collagen in simulated gastrointestinal fluids

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Food Bioscience 2023年第3期53卷 2616-2625页

作者： Yulun Chen Qingrun Liu Hang Yu Yahui Guo Yuliang Cheng He Qian Yunfei Xie weirong yao State Key Laboratory of Food Science and Technology Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina Engineering Research Center of Dairy Quality and Safety Control Technology Ministry of EducationInner Mongolia UniversityNo.235 Daxue West RoadHohhot010021Inner Mongolia Autonomous RegionChina School of Food Science and Technology Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina Joint International Research Laboratory of Food Safety Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina

Titanium dioxide nanoparticles(TiO_(2) NPs)could interact with proteins in foods and gastrointestinal fluids,thus changing the properties and functions of *** research articles have only studied the interactions between TiO_(2) NPs and digestive enzymes or the gastrointestinal fate of TiO_(2) NPs without considering the effect of food ***,the interactions between TiO_(2) NPs and trypsin and its effects on the digestion of collagen in vitro were *** showed that the functionalization of amino acids(Asp,and Ser)significantly harmed the adsorption of trypsin by TiO_(2) *** fluorescence quenching and isothermal titration calorimetry confirmed that the hydrogen bond and van der Waals force played an essential role in the interactions between TiO_(2) NPs and ***β-sheet content of trypsin bound to TiO_(2) NPs decreased from 52.40%to 50.73%while the random coil content increased from 20.20%to 22.63%.Binding sites were identified by molecular ***,the digestibility of collagen was improved in vitro in the presence of TiO_(2) NPs analyzed by SDS-PAGE,hydroxyproline quantification,and the antioxidation of digested *** concentra-tion increased by nearly 25%and ABTS radical scavenging rate increased by 50%.This study provides a new insight into the impact of nanoparticles on gastrointestinal digestion.

关键词： TiO_(2)nanoparticles Protein corona Gastrointestinal digestion

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淀粉性质及预处理对多孔淀粉形成的影响

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中国粮油学报 2005年第5期20卷 51-56页

作者：姚卫蓉姚惠源江南大学食品学院无锡214036

本文主要考察原料淀粉的性质及其预处理方式对多孔淀粉形成的影响。首先考察了不同来源的淀粉对形成多孔淀粉的影响,发现玉米和稻米淀粉适合制备多孔淀粉;接着考察了7种不同直链淀粉含量的稻米淀粉对制备多孔淀粉的影响,发现直链淀粉含... 详细信息

本文主要考察原料淀粉的性质及其预处理方式对多孔淀粉形成的影响。首先考察了不同来源的淀粉对形成多孔淀粉的影响,发现玉米和稻米淀粉适合制备多孔淀粉;接着考察了7种不同直链淀粉含量的稻米淀粉对制备多孔淀粉的影响,发现直链淀粉含量与水解率在1%水平上呈显著负相关。原料粒径越小,溶解度越大,多孔淀粉的吸油率越大,得率越低。淀粉中蛋白质含量高,酶解速度慢,但差别不显著,形成多孔淀粉的吸油率低;干法粉碎样品的起始反应速度要高于湿法粉碎样品的,但酶解后期,酶解速度与粉碎方式无关。

关键词：多孔淀粉直链淀粉粒径蛋白粉碎淀粉形成预处理方式淀粉性质直链淀粉含量反应速度

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多酚复合材料的种类及应用研究进展

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食品科学 2021年第13期42卷 300-308页

作者：姜楠姚卫蓉高媛王蒙北京农业质量标准与检测技术研究中心农业农村部农产品质量安全风险评估实验室(北京)北京100097 江南大学食品学院江苏无锡214122

多酚复合材料是多酚与某种配合物通过共价键或非共价键相互作用合成的新型复合体。与多酚结合的配合物种类多样,尤以多糖、蛋白质、磷脂等生物大分子以及金属元素居多。通过合成多酚复合材料可显著改善某些天然多酚水溶性差、稳定性差... 详细信息

多酚复合材料是多酚与某种配合物通过共价键或非共价键相互作用合成的新型复合体。与多酚结合的配合物种类多样,尤以多糖、蛋白质、磷脂等生物大分子以及金属元素居多。通过合成多酚复合材料可显著改善某些天然多酚水溶性差、稳定性差、生物利用率低等问题,不仅可以增强多酚作为口服营养素或药物的靶向释放,还可以用作体外植物源抑菌剂,很大程度上扩大了多酚在食品、药品、农产品质量安全等领域的应用范围。本文在总结近年来多酚复合材料最新研究结果的基础上,综述了多酚复合材料的不同类型、结构表征和功能性质以及应用现状,最后针对多酚复合材料在目前研究中存在的问题以及今后的重点研究方向提出了合理建议。

关键词：多酚物质复合材料功能特性应用现状

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A novel malachite green staining approach for evaluating the internal freshness of egg by detecting the quality of egg cuticle

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Food Bioscience 2022年第6期50卷 814-822页

作者： Zixuan Xu Yuliang Liu Fangwei Yang Lijun Huang Hang Yu weirong yao Yunfei Xie State Key Laboratory of Food Science and Technology Jiangnan University1800 Lihu AvenueWuxiJiangsu214122China School of Food Science and Technology Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province Jiangnan UniversityNo.1800 Lihu AvenueWuxi214122Jiangsu ProvinceChina Wuxi Food Safety Inspection and Test Center 35-210 Changjiang South RoadWuxi214142Jiangsu ProvinceChina

The cuticle is the outermost protective film of eggs, whose quality will affect internal freshness. At present, the undamaged detection of eggs freshness relies on hyperspectral imaging technology, which is relatively complicated. In this study, a novel simple approach for evaluating internal freshness of eggs was further explored by detecting the quality of egg cuticle. Malachite green selected as the alternative dye for quality evaluation of adhesive film, the correlation analysis was carried out by collecting the physical and chemical indexes of eggs with different degrees of freshness. It is found that there was a strong positive correlation between the quality of all parts of the cuticle and internal freshness (p < 0.01), and the Pearson correlation coefficient was over 0.85. The Haugh unit and pH of albumen were most strongly correlated with cuticle quality at the top, with the correlation coefficient of 0.919 and −0.906, respectively. The cuticle quality of eggs with contents got worse than that of eggs without contents, and the holes increased when they were stored for 28 days. In this way, malachite green staining method can be used to identify the freshness of egg cuticle, and the quality of egg cuticle can be used to distinguish eggs with different freshness. This study provided a new direction for the study of egg freshness and assisted to distinguish the quality and safety of eggs nondestructively.

关键词： Cuticle Egg freshness Bidirectional effect Quality evaluation Correlation analysis

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蜂蜜对苹果酶促褐变的抑制及有效成分分析

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食品与生物技术学报 2024年第2期43卷 55-62页

作者：黄欣莹姚卫蓉江南大学食品学院江苏无锡214122

作者以防止新鲜苹果切口的褐变为目标,比对不同食品配料组成的复配成分,发现蜂蜜作为涂膜材料可以有效延缓鲜切苹果片的褐变。将鲜切苹果片浸泡在质量分数0.1 g/g的椴树蜂蜜水溶液中,8 d后其仍能维持新鲜的色泽;探究了蜂蜜对苹果多酚氧... 详细信息

作者以防止新鲜苹果切口的褐变为目标,比对不同食品配料组成的复配成分,发现蜂蜜作为涂膜材料可以有效延缓鲜切苹果片的褐变。将鲜切苹果片浸泡在质量分数0.1 g/g的椴树蜂蜜水溶液中,8 d后其仍能维持新鲜的色泽;探究了蜂蜜对苹果多酚氧化酶(polyphenol oxidase,PPO)抑制的动力学,发现蜂蜜对苹果PPO起非竞争性抑制作用,酶活抑制能力来自蜂蜜的抗氧化、清除自由基(相关系数r>0.6)能力;进一步分析蜂蜜成分与酶活抑制率的关系,发现蜂蜜中蛋白质与多酚(r值分别为0.79、0.63)是蜂蜜发挥抗褐变作用的重要成分。

关键词：蜂蜜鲜切苹果片多酚氧化酶抗褐变

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多孔淀粉研究 Ⅱ淀粉开孔后的物性变化

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中国粮油学报 2005年第1期20卷 17-21页

作者：姚卫蓉姚惠源江南大学食品学院无锡214036

本文详细研究了玉米和籼米淀粉在形成多孔结构后的物性变化,发现淀粉开孔后,除了吸油率提高,堆积密度下降以外;平均粒径降低;比表面积增大;糊化性能基本与原淀粉一致,糊化温度、吸热焓没有明显变化,但糊化温度范围变窄;多孔淀粉基本... 详细信息

本文详细研究了玉米和籼米淀粉在形成多孔结构后的物性变化,发现淀粉开孔后,除了吸油率提高,堆积密度下降以外;平均粒径降低;比表面积增大;糊化性能基本与原淀粉一致,糊化温度、吸热焓没有明显变化,但糊化温度范围变窄;多孔淀粉基本仍保持原淀粉的结晶形态,但结晶度下降;另外,直链淀粉含量下降。这些特性数据的获得将有助于有效应用多孔淀粉。

关键词：多孔淀粉原淀粉糊化温度籼米淀粉堆积密度结晶度物性吸油率开孔平均粒径

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