检索结果-南通市图书馆

RNAi-mediated knock-down of Dab and Numb attenuate Ab levels via g-secretase mediated APP processing

Translational Neurodegeneration 2012年第1期1卷 45-54页

作者： Zhongcong Xie Yuanlin Dong Uta Maeda weiming xia Rudolph E Tanzi Department of Neurology Genetics and Aging Research UnitMassGeneral Institute for Neurodegenerative DiseaseMassachusetts General Hospital and Harvard Medical SchoolCharlestownMA 02129-2060USA Department of Anesthesia and Critical Care Geriatric Anesthesia Research UnitMassachusetts General Hospital and Harvard Medical SchoolCharlestownMA 02129-2060USA Center for Neurological Diseases Harvard Institute of Medicine and Harvard Medical SchoolBostonMA 02115USA Graduate student Department of PsychologyUniversity of Southern CaliforniaLos AngelesCA 90089USA.

Amyloid-b-protein(Ab),the key component of senile plaques in Alzheimer’s disease(AD)brain,is produced from amyloid precursor protein(APP)by cleavage of b-secretase and then *** adaptor proteins with phosphotyrosine-binding(PTB)domains,including Dab(gene:DAB)and Numb(gene:NUMB),can bind to and interact with the conserved YENPTY-motif in the APP *** we describe,for the first time,the effects of RNAi knock-down of Dab and Numb expression on APP processing and Ab *** knock-down of Dab and Numb in H4 human neuroglioma cells stably transfected to express either FL-APP(H4-FL-APP cells)or APP-C99(H4-APP-C99 cells)increased levels of APP-C-terminal fragments(APP-CTFs)and lowered Ab levels in both cell lines by inhibiting g-secretase cleavage of ***,RNAi knock-down of APP also reduced levels of Numb in H4-APP *** findings suggest that pharmacologically blocking interaction of APP with Dab and Numb may provide novel therapeutic strategies of *** notion of attenuating g-secretase cleavage of APP via the APP adaptor proteins,Dab and Numb,is particularly attractive with regard to therapeutic potential,given that side effects of gsecretase inhibition owing to impaired proteolysis of other g-secretase substrates,***,might be avoided.

关键词： APP impaired attractive

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

INFLUENCE OF THE DIFFERENT GEOMETRIC CONDITIONS ON X-RAY INTENSITIES OF SAMPLES

引用

电子显微学报 1991年第2期 201-202页

作者： ZHANG Renji, xia weiming, MA xiaohua(Tsinghua University)

In order to develop the new quantitative EDS analysis programfor the rough surfaces, the influence of the different geometricconditions on the X-ray intensities of samples has been *** found this kind of influence is ... 详细信息

关键词： AIK INFLUENCE OF THE DIFFERENT GEOMETRIC CONDITIONS ON X-RAY INTENSITIES OF SAMPLES

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

Chronic treatment with anesthetic propofol attenuates β-amyloid protein levels in brain tissues of aged mice

引用

Translational Neurodegeneration 2014年第1期3卷 46-52页

作者： Yiying Zhang Haijun Shao Yuanlin Dong Celeste A Swain Buwei Yu weiming xia Zhongcong Xie Geriatric Anesthesia Research Unit Department of AnesthesiaCritical Care and Pain MedicineMassachusetts General Hospital and Harvard Medical School14913th St.Room 4310CharlestownMA 02129-2060USA Department of Anesthesiology Ruijin HospitalShanghai Jiao Tong University School of Medicine200025 ShanghaiP.R.China Department of Veterans Affairs Medical Research and Development Service and Geriatric ResearchEducation and Clinical CenterBedfordMA 01730UK

Alzheimer’s disease(AD)is the most common form of *** the present time,however,AD still lacks effective *** recent studies showed that chronic treatment with anesthetic propofol attenuated brain caspase-3 activation and improved cognitive function in aged *** ofβ-amyloid protein(Aβ)is a major component of the neuropathogenesis of AD dementia and cognitive *** therefore set out to determine the effects of chronic treatment with propofol on Aβlevels in brain tissues of aged ***(50 mg/kg)was administrated to aged(18 month-old)wild-type mice once a week for 8 *** brain tissues of mice were harvested one day after the final propofol *** harvested brain tissues were then subjected to enzyme-linked immunosorbent assay(ELISA)and Western blot *** we report that the propofol treatment reduced Aβ(Aβ40 and Aβ42)levels in the brain tissues of the aged ***,the propofol treatment decreased the levels ofβ-site amyloid precursor protein cleaving enzyme(the enzyme for Aβgeneration),and increased the levels of neprilysin(the enzyme for Aβdegradation)in the brain tissues of the aged *** results suggested that the chronic treatment with propofol might reduce brain Aβlevels potentially via decreasing brain levels ofβ-site amyloid precursor protein cleaving enzyme,thus decreasing Aβgeneration;and via increasing brain neprilysin levels,thus increasing Aβ*** preliminary findings from our pilot studies have established a system and postulated a new hypothesis for future research.

关键词： Aging Alzheimer’s disease Neurodegeneration β-amyloid peptide Anesthesia Propofol

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Multimodal comparison of plasma proteins associated with blood-brain barrier impairment in Alzheimer’s disease

引用

Aging Communications 2024年第1期6卷 1-9页

作者： Elisa Giunti Roberto Collu Joel Reisman Jong Soo Lee Noureddine Melikechi weiming xia Geriatric Research Education and Clinical Center Bedford VA Healthcare SystemBedfordMA 01730U.S.A Department of Pharmacology Physiology and BiophysicsBoston University Chobanian&Avedisian School of MedicineBostonMA 02118U.S.A Center for Healthcare Organization and Implementation Research Bedford VA Healthcare SystemBedfordMA 01730U.S.A Kennedy College of Science University of Massachusetts LowellLowellMA 01854U.S.A.

Background:Vascular impairment is one of the major contributors to *** aimed to identify blood biomarkers suggestive of potential impairment of the blood-brain barrier(BBB)in subjects with Alzheimer’s disease(AD).Methods:We used administrative data from the VA Informatics and Computing Infrastructure Resource Center to study both inpatients and outpatients with *** samples from healthy control and AD individuals were analyzed using enzyme-linked immunosorbent assay and proteomics approaches to identify differentially expressed *** analysis was applied to explore significantly enriched ***:In the same cohort of patients with AD,we found twice number of subjects with cerebral amyloid angiopathy in the two-year period after the onset of AD,compared to the number of subjects with cerebral amyloid angiopathy in the two-year period prior to AD *** pathways related to BBB,like cell adhesion,extracellular matrix organization and Wnt signaling,were activated and differentially expressed proteins such as ADAM22,PDGFR-α,DKK-4,Neucrin and RSOP-1 were ***,matrix metalloproteinase-9,which is implicated in causing degradation of basal lamina and BBB disruption,was significantly increased in the plasma of AD ***:Alteration of proteins found in AD subjects could provide new insights into biomarkers regulating permeability and BBB integrity.

关键词： Alzheimer’s disease blood-brain barrier peripheral biomarkers matrix metalloproteinases cerebral amyloid angiopathy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

在役检查方法对INCONEL-600镍基合金焊缝应力腐蚀裂纹的检出能力分析

引用

核动力工程 2015年第S1期36卷 130-134页

作者：夏炜铭何子昂许峰孙军中核核电运行管理有限公司浙江海盐314300 中国核动力研究设计院成都610213

以秦山核电厂300 MW级核电机组一回路主系统INCONEL-600镍基合金材料焊缝为研究对象,分析秦山核电厂现用在役检查检验方法和检验频度在INCONEL-600合金材料焊缝应力腐蚀裂纹检出能力方面的不足,提出针对一回路主系统INCONEL-600镍基合... 详细信息

以秦山核电厂300 MW级核电机组一回路主系统INCONEL-600镍基合金材料焊缝为研究对象,分析秦山核电厂现用在役检查检验方法和检验频度在INCONEL-600合金材料焊缝应力腐蚀裂纹检出能力方面的不足,提出针对一回路主系统INCONEL-600镍基合金材料焊缝检验的应对措施。

关键词： INCONEL-600镍基合金焊缝在役检查应力腐蚀裂纹

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Platelet function monitoring guided antiplatelet therapy in patients receiving high-risk coronary interventions

引用

Chinese Medical Journal 2014年第19期127卷 3364-3370页

作者： Xu Li Wang Lefeng Yang Xinchun Li Kuibao Sun Hao Zhang Dapeng Wang Hongshi Li weiming Ni Zhuhua xia Kun Liu Yu Heart Center Beijing Chaoyang.Hospital CapitalMedicl UniversityBeijing 100020

Background Large-scale clinical trials have shown that routine monitoring of the platelet function in patients after percutanous coronary intervention （PCI） is not necessary. However, it is still unclear whether patients received high-risk PCI would benefit from a therapy which is guided by a selective platelet function monitoring. This explanatory study sought to assess the benefit of a therapy guided by platelet function monitoring for these patients. Methods Acute coronary syndrome （ACS） patients （n=384） who received high-risk, complex PCI were randomized into two groups. PCI in the two types of lesions described below was defined as high-risk, complex PCI： lesions that could result in severe clinical outcomes if stent thrombosis occurred or lesions at high risk for stent thrombosis. The patients in the conventionally treated group received standard dual antiplatelet therapy. The patients in the platelet function monitoring guided group received an antiplated therapy guided by a modified thromboelastography （TEG） platelet mapping： If inhibition of platelet aggregation （IPA） induced by arachidonic acid （AA） was less than 50% the aspirin dosage was raised to 200 mg/d; if IPA induced by adenosine diphosphate （ADP） was less than 30% the clopidogrel dosage was raised to 150 mg/d, for three months. The primary efficacy endpoint was a composite of myocardial infarction, emergency target vessel revascularization （eTVR）, stent thrombosis, and death in six months. Results This study included 384 patients; 191 and 193 in the conventionally treated group and platelet function monitoring guided group, respectively. No significant differences were observed in the baseline clinical characteristics and interventional data between the two groups. In the platelet function monitoring guided group, the mean IPA induced by AA and ADP were （69.2＋24.5）% （range, 4.8% to 100.0%） and （51.4＋29.8）% （range, 0.2% to 100.0%）, respectively. The AA- induced IPA of forty-three （22.2%） patients wa

关键词： platelet function antiplatelet therapy, percutanous coronary intervention, acute coronary syndrome

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

美托洛尔联合曲美他嗪治疗冠心病心力衰竭的疗效观察及对患者血清炎性因子的影响

引用

中国基层医药 2020年第12期27卷 1482-1486页

作者：阮政文夏伟明余姚市人民医院心内科浙江省315400

目的观察美托洛尔联合曲美他嗪治疗冠心病心力衰竭的疗效及对患者血清炎性因子的影响。方法选择余姚市人民医院2018年7月至2019年7月收治的冠心病心力衰竭患者182例,按照随机数字表法分为观察组91例与对照组91例。对照组患者给予曲美他... 详细信息

目的观察美托洛尔联合曲美他嗪治疗冠心病心力衰竭的疗效及对患者血清炎性因子的影响。方法选择余姚市人民医院2018年7月至2019年7月收治的冠心病心力衰竭患者182例,按照随机数字表法分为观察组91例与对照组91例。对照组患者给予曲美他嗪治疗,观察组在对照组基础上联合美托洛尔治疗。两组疗程均为3个月。比较两组治疗疗效,治疗前后心功能、炎性因子、血浆B型尿钠肽(BNP)和6 min步行距离(6WMT)变化。结果观察组总有效率(91.21%)高于对照组(76.92%)(χ^2=6.933,P<0.05);观察组治疗后左室收缩末期内径(LVESD)[(45.78±4.35)mm和左室舒张末期内径(LVEDD)[(38.29±3.24)mm]低于对照组的(51.20±3.01)mm和(43.10±2.65)mm,而左室射血分数(LVEF)[(49.83±3.86)%]高于对照组的(43.25±2.69)%(t=9.774、10.962、13.341,均P<0.05);观察组治疗后血清肿瘤坏死因子α(TNF-α)[(4.35±1.30)mg/L]和超敏C反应蛋白(hs-CRP)[(2.63±0.54)mg/L]均低于对照组的(7.84±2.38)mg/L和(3.98±0.76)mg/L(t=12.276、13.813,均P<0.05);观察组治疗后血浆BNP[(317.94±24.35)pg/mL]低于对照组的(384.35±18.97)pg/mL,而6WMT[(341.32±35.41)m]高于对照组[(289.78±25.45)m](t=20.524、11.275,均P<0.05)。结论美托洛尔联合曲美他嗪治疗冠心病心力衰竭患者疗效明显,且可明显改善患者心功能,减轻炎性反应。

关键词：冠状动脉疾病心力衰竭肿瘤坏死因子α C反应蛋白质疗效比较研究美托洛尔曲美他嗪

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

等剪应力钢丝缠绕缸筒的有限元模拟与应用

引用

锻压技术 2020年第9期45卷 201-211页

作者：杨晓俊夏卫明常州刘国钧高等职业技术学校机电工程系江苏常州213025 江苏省徐州锻压机床厂集团有限公司江苏徐州221100

结合厚壁缸筒等剪应力钢丝缠绕理论,给出了厚壁缸筒等剪应力缠绕的有限元模拟方法。由于有限元模拟和实际缠绕施工中,对钢丝层按多个等厚度的台阶进行简化,使得缠绕后各钢丝台阶中的剪应力比理论值略低,实际的预紧效果略低于理论设计。... 详细信息

结合厚壁缸筒等剪应力钢丝缠绕理论,给出了厚壁缸筒等剪应力缠绕的有限元模拟方法。由于有限元模拟和实际缠绕施工中,对钢丝层按多个等厚度的台阶进行简化,使得缠绕后各钢丝台阶中的剪应力比理论值略低,实际的预紧效果略低于理论设计。根据各钢丝台阶剪应力有限元解和理论解的差值,提出了误差率的概念,并给出了误差补偿措施,经过误差补偿后的有限元模型,有限元解和理论解一致。讨论了预紧系数的意义和取值范围,并结合50 MN预应力钢丝缠绕液压缸的设计,研究了在最小预紧系数和极限预紧系数的范围内,初始缠绕应力随预紧系数的变化规律,以及预紧工况和合成工况下,芯筒内外壁径向、切向应力的变化规律。

关键词：等剪应力预应力预紧系数钢丝缠绕缸筒切向应力

来源：

维普期刊数据库

同方期刊数据库评论