Chronic treatment with anesthetic propofol attenuates β-amyloid protein levels in brain tissues of aged mice

作     者：Yiying Zhang Haijun Shao Yuanlin Dong Celeste A Swain Buwei Yu Weiming Xia Zhongcong Xie 

作者机构：Geriatric Anesthesia Research UnitDepartment of AnesthesiaCritical Care and Pain MedicineMassachusetts General Hospital and Harvard Medical School14913th St.Room 4310CharlestownMA 02129-2060USA Department of AnesthesiologyRuijin HospitalShanghai Jiao Tong University School of Medicine200025 ShanghaiP.R.China Department of Veterans AffairsMedical Research and Development Service and Geriatric ResearchEducation and Clinical CenterBedfordMA 01730UK 

出 版 物：《Translational Neurodegeneration》 (转化神经变性病（英文）)

年 卷 期：2014年第3卷第1期

页      面：46-52页

学科分类：1002[医学-临床医学] 10[医学] 

基　　金：This research was supported by R21AG038994 RO1 GM088801 and RO1AG041274 from National lnstitutes of Health Bethesda Maryland Investigator-initiated Research grant from Alzheimer's Association Chicago llinois and Cure Alzheimer's Fund Wellesley Massachusetts to Zhongcong Xie 

主　　题：Aging Alzheimer’s disease Neurodegeneration β-amyloid peptide Anesthesia Propofol 

摘      要：Alzheimer’s disease(AD)is the most common form of *** the present time,however,AD still lacks effective *** recent studies showed that chronic treatment with anesthetic propofol attenuated brain caspase-3 activation and improved cognitive function in aged *** ofβ-amyloid protein(Aβ)is a major component of the neuropathogenesis of AD dementia and cognitive *** therefore set out to determine the effects of chronic treatment with propofol on Aβlevels in brain tissues of aged ***(50 mg/kg)was administrated to aged(18 month-old)wild-type mice once a week for 8 *** brain tissues of mice were harvested one day after the final propofol *** harvested brain tissues were then subjected to enzyme-linked immunosorbent assay(ELISA)and Western blot *** we report that the propofol treatment reduced Aβ(Aβ40 and Aβ42)levels in the brain tissues of the aged ***,the propofol treatment decreased the levels ofβ-site amyloid precursor protein cleaving enzyme(the enzyme for Aβgeneration),and increased the levels of neprilysin(the enzyme for Aβdegradation)in the brain tissues of the aged *** results suggested that the chronic treatment with propofol might reduce brain Aβlevels potentially via decreasing brain levels ofβ-site amyloid precursor protein cleaving enzyme,thus decreasing Aβgeneration;and via increasing brain neprilysin levels,thus increasing Aβ*** preliminary findings from our pilot studies have established a system and postulated a new hypothesis for future research.